• Pediatric Infection and Vaccine
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• v.12 no.1
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• pp.52-60
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• 2005

Purpose : The aim of this study is to know seasonal distribution of group A streptococci obtained from one center using emm genotyping and T serotyping in Masan from 2003 through 2004. Methods : Among children who visited the Changwon Fatima Hospital at Masan, Korea from June 2003 through February 2004, 100 patients who had clinical findings of acute pharyngitis, scarlet fever, and cellulitis were confirmed as GAS by culture, and were enrolled in our study. All obtained GAS were sent to the WHO Collaborative Center for Reference and Research on Streptococci, University of Minnesota, Minneapolis for T serotyping and emm genotyping. We classified these results again according to seasonal and disease's entities. Results : 19 different T serotypes was typed. T4(27.5%), T1(17.6%), T6(13.7%), and T12(13.7%) serotypes were relatively common in summer, while T4(28.3%), T12(15.2%), and T12/B3264(8.7%) were common in winter. T4 and T12 were persistent all year around. Distribution of T serotypes in 89 patients with pharyngotonsillitis were T4(26.7%), T12(14.0%), T1(12.8%), and T6(11.6%) in order of frequency. 15 different emm genotypes was typed. The number of emm 1, emm 6, emm 9, and emm 44 genotypes decreased or disappeared in winter, and the number of emm 3, emm 12, and emm 89 genotypes increased or reappeared in winter. Conclusion : Because T serotyping and emm genotyping are useful tools for evaluating epidemiology and pathogenesis of group A streptococci, we should monitor these strains every year, and should serotype and genotype GAS obtained from the invasive streptococcal infections.

• Horticultural Science & Technology
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• v.28 no.4
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• pp.664-671
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• 2010

A genetic linkage map was constructed using 188 $F_9$ RILs derived from a cross between $Solanum$ $lycopersicum$ H7996 (resistant to bacterial wilt) and $S.$ $pimpinellifolium$ WVa700 (highly susceptible to bacterial wilt). The map consisted of 361 markers including 260 DArTs, 74 AFLPs, 4 RFLPs, 1 SNP, and 22 SSRs. The resulting linkage map was comprised of 13 linkage groups covering 2042.7 cM. The genetic linkage map had an average map distance between markers of 5.7 cM, with an average DArT marker density of 1/7.9 cM. Based on the distribution of anchor SSR markers, 11 linkage groups were assigned to 10 chromosomes of tomato except chromosomes 5 and 12. The DArT markers were distributed across the genome in a similar way as other markers and showed the highest frequency of clustering (38.8%) at ${\leq}$ 0.5 cM intervals between adjacent markers, which is 3 times higher than AFLPs (13.5%). The present study is the first utilization of DArT markers in tomato linkage map construction.

• Investigative Magnetic Resonance Imaging
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• v.16 no.2
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• pp.103-114
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• 2012

Purpose : In an attempt to further improve the radiofrequency (RF) magnetic ($B_1$) field strength in temporomandibular joint (TMJ) imaging, a 4-channel spiral-loop coil array with RF circuitry was designed and compared with a 4-channel single-loop coil array in terms of $B_1$ field, RF transmit (${B_1}^+$), signal-to-noise ratio (SNR), and applicability to TMJ imaging in 7T MRI. Materials and Methods: The single- and 4-channel spiral-loop coil arrays were constructed based on the electromagnetic (EM) simulation for the investigation of $B_1$ field. To evaluate the computer simulation results, the $B_1$ field and ${B_1}^+$ maps were measured in 7T. Results: In the EM simulation result and MRI study at 7T, the 4-channel spiral-loop coil array found a superior $B_1$ performance and a higher ${B_1}^+$ profile inside the human head as well as a slightly better SNR than the 4-channel single-loop coil array. Conclusion: Although $B_1$ fields are produced under the influence of the dielectric properties of the subject rather than the coil configuration alone at 7T, each RF coil exhibited not only special but also specific characteristics that could make it suited for specific application such as TMJ imaging.

• The Journal of Dong Guk Oriental Medicine
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• v.6 no.2
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• pp.99-107
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• 1998

Cyclosporin A(CsA) is a selective immunosuppressive agent that has been credited with improved survival of solid organ allografts. Lymph node of BALB/C mouse administered CsA immunohistochemically observed to understand immunosuppressive effects of CsA on T lymphocytes, IL-2 receptors, and natural killer NK cells in lymph node. CsA orally administered daily for 10days at the dose 45mg/kg/day/. The lymph node were obtained at day 3, 7, and 14 after CsA administration and embedded with paraffin, and then stained by following ABC method that used monoclonal antibody including L3T4(CD4), Ly2(CD8), IL-2R(CD25), and NK-1.1(CD56). There were little changes of reactive degree and number of helper T lymphocytes, cytotoxic T lymphocytes, IL-2 receptors, and NK cells at day 3 after CsA administration, but they began to decrease at day 7. These decrease were greatest at day 14. The helper T lymphocytes. cytotoxic T lymphocytes, IL-2 receptors, and NK cells distributed in paracortex and medullary sinus. These results indicated that the secretion of IL-2 began to decrease at day 7 after CsA administration and subsequently to suppress T lymphocytes and NK cell as components of cell-mediated immunity.

• Journal of the Korean Magnetics Society
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• v.9 no.6
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• pp.271-277
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• 1999

Amorphous $Fe_{83}B_9Nb_7Cu_1$ alloy has been studied by M$\"{o}$ssbauer spectroscopy. Revised Vincze method was used and distributions of hyperfine field, isomer shift, and quadrupole line broadening of the sample at various temperatures have been evaluated and Curie temperature and $H_{hf}\;(0)$ were calculated to be 393 K and 231 kOe, respectively. Temperature variation of reduced average hyperfine field shows a flattered curvein comparison with the Brillouin curve for S=1. This behavior can be explained on the basis of Handrich molecular field model, in which the parameter Δ, which is a measure of fluctuation in exchange interactions, is assumed to have the temperature dependence ${Delta}=0.75-0.64{\tau}+0.47{\tau}^2$ where $\tau$ is $T/T_C$. At low temperature, the average hyperfine field can be fitted to $H_{hf}\;(T)=H_{hf}\;(0)\;[1-0.44\;(T/T_C)^{3/2}-0.28(T/T_C)^{5/2}-… ]$, which indicates the presence long wave length spin wave excitations. At temperature near TC, reduced average hyperfine field varies as $1.00\;[1-T/T_C]^{0.39}$. It is also found that half-width of the hyperfine field distribution was 102 kOe (3.29 mm/s) at 13 K and decreased monotonically as temperature increased. Above the Curie temperature, an average quadrupole splitting value of 0.43 mm/s was found. Average line broadening due to quadrupole splitting distribution was 0.31 mm/s at 13 K and decreases monotonically to 0.23 mm/s at 320 K, whereas that due to the isomer shift distribution is 0.1 mm/s at 13 K and 0.072 mm/s at 320 K, which is much smaller than that of both hyperfine field and quadrupole splitting. The temperature dependence of the isomer shift can be fitted within the harmonic approximation to a Deybe model with a Debye temperature ${Theta}_D=424{\pm}5K$.TEX>.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.3 no.3
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• pp.142-148
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• 1998

Environmental baseline information is necessary in order to assess the potential environmental impact of future manganese-nodule mining on the deep-seabed ecosystem. Total ATP (T-ATP), dissolved ATP (D-ATP) and particulate ATP (P-ATP) were measured to estimate total microbial biomass and to elucidate their vertical distribution patterns in the seabed of KODOS (Korea Deep Ocean Study) area, northeast equatorial Pacific Ocean. Within the upper 6 cm depth of sediment, the concentrations of T-ATP, D-ATP and P-ATP ranged from 4.4 to 40.6, from 0.6 to 16.1, and from 3.0 to 29.2 ng/g dry sediment, respectively. Approximately 84% of T-ATP, 81% of D-ATP, and 74% of P-ATP were present within the topmost 2 cm depth of sediment, and the distributions of ATP were well correlated with water content in the sediment. These results indicate that the distribution of total microbial biomass was largely determined by the supply of organic matter from surface water column. Fine-scale vertical variations of ATP were detected within 1-cm thick veneer of the sediment samples collected by multiple corer, while no apparent vertical changes were observed in the box-cored samples. It is evident that the box-core samples were disturbed extensively during sampling, which suggests that the multiple corer is a more appropriate sampling gear for measuring fine-scale vertical distribution pattern of ATP within thin sediment veneer. Overall results suggest that the concentrations of ATP, given their clear changes in vertical distribution pattern within 6 cm depth of sediment, are a suitable environmental baseline parameter in evaluating the variations of benthic microbial biomass that are likely to be caused by deep-seabed mining operation.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.13 no.3
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• pp.145-152
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• 2001

Low Rayleigh number thermal convection in a fluid layer confined between two-infinite horizontal walls kept at spatially sinusoidal temperature distributions, T_L=T_m+\Delta T\sin \kappax,\;T_U=T_m+\Delta T\sin(\kappax-\beta)$, is theoretically investigated by a regular perturbation expansion method. For small wave numbers, an upright cell is formed between the two walls at $\beta$=0. The cell is tilted, as the phase difference increases, and a flow with tow counter-rotating eddies occurs at $\beta=\pi$. when the wave number is large, isolated eddies are formed near the lower and upper walls, for all the phase differences. There exists a wave number at which maximum heat transfer rate at the walls occurs, at each of the phase differences. And the wave number increases with increase of the phase difference. for a fixed wave number, the heat transfer rate decrease with increase of the phase difference.

• Proceedings of the Korea Information Processing Society Conference
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• 2003.05a
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• pp.405-408
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• 2003

영상의 구성물질에 따른 정확한 분할은 질병의 유무를 판단하는데 매우 중요하다. 그러나 영상에서 구성물질들을 정확하게 분할하기란 쉬운 문제가 아니다. 그리고 많은 연구들이 뇌의 실질적인 량을 고려하지 못한 상태서 분할이 이루어지고 있다. 따라서 뇌의 실질적인 량과 비교할 때 가장 근접한 방법 의 개발이 필요하다고 볼 수 있다. 본 논문은 fat을 소거한 T2 영상과 T1 영상을 이용하여 조직에 따르는 명암 분포가 각각 다르게 분포되어 있는 것을 이용하여 평활화한 후 두 영상의 차로 백질, 회백질, 뇌척수액을 분리하는 방법을 제안한다. 이 방법을 이용하여 정상이의 뇌 MR 영상 이용하여 (19 Slice) 백질, 회백질, 뇌척수액을 분리하는 방법을 제시하였다.

• The Korean Journal of Nuclear Medicine
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• v.32 no.4
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• pp.325-331
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• 1998

Purpose: We evaluated the importance of redistribution and 24 hour reinjection images in T1-201 SPECT assessment of myocardial viability after acute myocardial infarction (AMI). Materials and Methods: We performed dipyridamole stress-4 hour redistribution-24 hour reinjection T1-201 SPECT in 43 patients with recent AMI (4-16 days). The myocardium was divided into 16 segments and perfusion grade was measured visually with 4 point score from 0 to 3 (absent uptake to normal uptake). A perfusion defect with stress score 2 was considered moderate. A defect was considered severe if the stress score was 0 or 1 (absent uptake or severe perfusion decrease). Moderate defect on stress image were considered viable and segments with severe defect were considered viable if they showed improvement of 1 score or more on redistribution or reinjection images. We compared the results of viability assessment in stress-redistribution and stress-reinjection images. Results: On visual analysis, 344 of 688 segments (50%) had abnormal perfusion. Fifty two (15%) had moderate perfusion defects and 292 (85%) had severe perfusion defects on stress image. Of 292 severe stress defects, 53 were irreversible on redistribution and reversible on reinjection images, and 15 were reversible on redistribution and irreversible on reinjection images. Two hundred twenty four of 292 segments (76.7%) showed concordant results on stress-redistribution and stress-reinjection images. Therefore 24 hour reinjection image changed viability status from necrotic to viable in 53 segments of 292 severe stress defect (18%). However, myocardial viability was underestimated in only 5% (15/292) of severe defects by 24 hour reinjection. Conclusion: The 24 hour reinjection imaging is useful in the assessment of myocardial viability. It is more sensitive than 4 hour redistribution imaging. However, both redistribution and reinjection images are needed since they complement each other.

• The Korean Journal of Nuclear Medicine
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• v.30 no.4
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• pp.493-501
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• 1996

To assess contribution of T1-201 rest-24 hour delay redistribution in detection of viable myocardium, we studied the predictive value of this redistribution in 17 patients who peformed rest-24 hour delay perfusion SPECT before bypass surgery. Regional wall motion was compared with gated SPECT in 10 patients and echocardiography in 7 patients before and after bypass surgery. Rest and 24 hour delayed uptakes were scored from 0 (normal perfusion) to 3 (defect). In rest SPECT, 56 segments showed perfusion decrease. Thirty four segments(61%) improved after surgery and were defined as viable Nineteen(34%) segments had more uptake of T1-201 at 24 hour delay, and the other 37 segments did not. In 81%(25/31) of segments with mildly decreased perfusion, wall motion after bypass surgery improved, 57% (8/14) of segments with severely decreased perfusion improved, and 9%(1/11) of segments with defects improved. In 14 among 19 segments which had more T1-201 uptakes at 24 hour delay, wall motion was improved(positive predictive value of redistribution: 74%). 20 among 37 segments which had persistent decreases in rest-24 hour redistribution improved and 17 did not(negative predictive value: 46%). Segments having severe perfusion decrease or defects showed improved wall motion after surgery in 64%(7/11), if it had redistribution at delay. Segments with either mildly decreased uptake in resting or rest-delayed redistribution showed improved wall motion in 76%(32/42). Among the 14 segments which showed improvement in wall motion, 10 had partial reversibility in stress-rest images and the other 4 had persistent perfusion defects in stress-rest images. These 4 segments were found viable only with rest-24 hour delayed perfusion SPECT. We concluded that rest T1-201 uptake or redistribution at 24 hour delay should be referred as an evidence to warrant postoperative improvement of abnormal wall motion and we could predict myocardial viability with preoperative rest-24 hour delay perfusion SPECT in the segments with rest perfusion decreases.