• Korean Journal of Pharmacognosy
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• v.50 no.3
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• pp.175-184
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• 2019

Mast cells are crucial as effector cells in the immediate-type allergic reaction. Lentinus edodes has been the popular edible mushroom in oriental countries and reported to have immunomodulatory, anti-tumor, anti-atherogenic, anti-viral, and anti-allergic activities. However, the roles of L. edodes in mast cell-mediated anaphylactic reaction have not been fully elucidated. In this research, we have demonstrated the effects of the methanol extract of L. edodes (MELE) on mast cell-mediated anaphylaxis-like and anaphylactic reactions. MELE suppressed systemic anaphylaxis-like reaction, plasma histamine levels, and ear swelling response in mice treated with compound 48/80. MELE also suppressed passive systemic and cutaneous anaphylaxis mediated by anti-dinitrophenyl IgE. In accordance with these findings, MELE dose-dependently decreased histamine release from RPMC evoked by compound 48/80 or the antigen-antibody reaction. To clarify the mechanism of degranulation system, intracellular cAMP levels as well as calcium influx in RPMC was evaluated. In compound 48/80-treated RPMC, MELE blocked calcium uptake into the cells. In addition, MELE elevated the intracellular cAMP content and significantly attenuated compound 48/80-induced cAMP reduction in RPMC. Taken together, we propose the clinical use of MELE in mast cell-mediated immediate-type allergic diseases.

• Biomolecules & Therapeutics
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• v.4 no.4
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• pp.334-338
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• 1996

Antigenicity of DA-3285, human recombinant erythropoietin which was produced from mammalian cells, was examined in guinea pigs and mice. In active systemic anaphylactic test, mild anaphylactic signs were observed in guinea pigs sensitized subcutaneously with DA-3285 or DA-3285 incorporated with complete Freund's adjuvant(CFA) when challenged with high dose(1000 IU/Kg) of DA-3285. Other groups showed negative responses. In mouse-rat passive cutaneous anaphylaxis test, 20% sera of mice immunized with DA-3285 or DA-3285 mixed with aluminum hydroxide(alum) showed mild positive responses. In the case of indirect haemagglutination reaction(IHA) test, when sheep red blood cells coated with DA-3285 was incubated with mouse serum, all the serum samples were showed negative responses. These results suggest that DA-3285 has a very weak antigenic potential and probably would not induce systemic allergic reactions in clinical uses.

• The Journal of Internal Korean Medicine
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• v.25 no.2
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• pp.159-166
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• 2004

Objective : Mast cells are a potent source of mediators that regulate inflammatory response in allergies and asthma. The author studied the effect of Bojungikgitanggamibang(BITB) on mast cell-mediated anaphylactic reaction. Method : When BITB was given as pre-treatment at concentrations ranging from 0.01 to 1 mg/ml, the histamine release from rat peritoneal mast cells induced by compound 48/80 was reduced in a dose-dependent manner. Result : BITB dose-dependently inhibited compound 48/80-induced systemic anaphylactic shock. BITB also inhibited passive cutaneous anaphylaxis activated by anti-dinitrophenyl IgE. In addition, BITB inhibited phorbol 12-myristate 13-acetate and A23187-induced interleukin-6 secretion from human mast cell line HMC-1 cells. Conclusion : These results indicate that BITB may be actively anti-allergic.

• The Journal of Korean Medicine
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• v.19 no.2
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• pp.439-449
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• 1998

Anal Therapy is another way of taking medicine. It is a traditional pathway but not available in common situation. Nevertheless, It has many benifect and usefulness, it has not treated so much. Through Anal Therapy, the valid compound of Herb med can be reach to the desination in theory of the organism and loca1 medical action. The former is called Jung-Chei Theory(整體論), which is the one of the most important basements in building traditional Korean medicine. As there are many kinds of Anal therapy, this study use reservation type. Sosihotang(SSHT) is one of the well-known korean medicines for a long time. It is used for the treatment of such dieases as infectious diseases, hepatic diseases and gastroenteritis and so on. In this study, the author investigated the effect of an aqueous extract of SSHT by Anal therapy(Reservative Enema) in anaphylactic shock. The following results were obtained 1. SSHT inhibited anaphylactic shock 100% with a dose of 1.0 g/kg 1 hr before intraperitoneal injection of compound 48/80. SSHT significantly reduced serum histamine contents induced by compound 48/80. 2. SSHT (0.1 g/kg) also inhibited to 30.9% (P<0.05)) local cutaneous anaphylactic reaction activated by anti-dinitrophenyl (DNP) IgE. 3. The validity rate of reservative enema is as much as oral pathway. 4. In addition, SSHT dose-dependently inhibited the histamine release from the peritoneal mast cells by compound 48/80 or anti-DNP IgE. These results provide evidence that Anal Therapy(Reservative enema) of SSHT may be beneficial in the treatment of systemic and local anaphylactic reaction. Moreover, I wish another much sincere study of Anal Therapy (Reservative enema) would be obtained.

• Advances in Traditional Medicine
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• v.4 no.3
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• pp.125-136
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• 2004

The pharmacological mechanisms of most Oriental medicines have not been clearly defined in spite of their effective use in treating many diseases throughout the world. Many Oriental medicines have been used against various allergic diseases for generations, and still occupy an important place in traditional medicine in far eastern countries including Korea. It is also still unclear how Oriental drugs prevent allergic disease in vivo or in vitro models. Some Korean folk medicine inhibited the mast cell-mediated allergic reaction. This review summarizes the effective folk medicine in experimental effect on systemic or local anaphylaxis reaction. Potential anti-anaphylactic folk medicines include: Poncirus trifoliata; Siegesbeckia glabrescence; Solanum lyratum; Aquilaria agallocha; Ulmi radicis; Polygonum tinctorium; Hwanglyun-Haedok-Tang; Rehmannia glutinosa; Kum-Hwag-San; Syzygium aromaticm; Spirulina platensis; Sosiho-Tang; Sinomenium acutum; Schizonepta tenuifolia; Shini-San; Magnoliae flos; Sochungryong-Tang; Oryza sativa; Cryptotympana atrata; Salviae radix; Rosa davurica; Asiasari radix; Chung-Dae-San; Cichorium intybus; Perilla frutescens; Vitex rotundifolia; Terminalia chebula; Siberian Ginseng; Solanum melongena; Gahmi-Shini-San; Alpinia oxyphylla; Acanthopanax senticosus root; Prunella vulgaris; Allergina; Ixeris dentate; Acanthopanax senticosus stem; Tongkyutang; Salvia plebeia; Rubus coreanus; Sinpo- Tang; Dodutang; Forsythia fructus; Xanthii fructus; and Purple bamboo slat. Ensuring the effects and understanding the mechanisms of action for these Oriental medicines can permit drug development and laying of the ground-work for evaluating potential synergistic effects by addition and subtraction of prescriptions.

• Advances in Traditional Medicine
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• v.2 no.1
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• pp.58-63
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• 2002

The effect of the aqueous extract of Rubus coreanus Miq. (Rosaceae) fruits (AERC) on anaphylactic reaction was investigated. AERC completely inhibited compound 48/80-induced systemic anaphylactic shock at dose of 0.1 to 1.0 g/kg. When AERC was pretreated at concentrations ranging from 0.01 to 1.0 g/kg, the plasma histamine levels induced by compound 48/80 were reduced in a dose-dependent manner. AERC also inhibited the histamine release from rat peritoneal mast cells (RPMC) by compound 48/80. The level of cAMP in RPMC, when AERC (1.0 mg/ml) was added, transiently and significantly increased about 6-fold compared with that of basal cells. These results indicate that AERC may be beneficial in the treatment of non-specific anaphylactic reactions.

• Kosin Medical Journal
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• v.33 no.3
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• pp.468-476
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• 2018

Perioperative anaphylaxis, although rare, is a severe, life-threatening unexpected systemic hypersensitivity reaction. Simultaneous administration of various drugs during anesthesia, the difficulty of communicate with patients in sedation and anesthesia, and coverage of the patient with surgical drapes are considered to be factors that impede early recognition of anaphylactic reactions. It is very important to perform an intradermal skin test because antibiotics are the most common cause of perioperative anaphylaxis. We report a case of negative-intradermal skin test antibiotic anaphylaxis mistaken for local aesthetic systemic toxicity without increase of serum tryptase for confirmative diagnostic biomaker during surgery under brachial plexus block. It is not possible to exclude the danger of anaphylaxis completely, even if it is negative-intradermal skin test and normal tryptase level. Therefore, anesthesiologists should be closely monitored and treated early for antibiotics related hypersensitive reaction, like other medicines during anesthesia.

• Korean Journal of Clinical Pharmacy
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• v.22 no.3
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• pp.268-273
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• 2012

The definition of anaphylaxis is 'a serious, life-threatening generalized or systemic hypersensitivity reaction' and is considered as the life threatening adverse drug reaction. We experienced a case of cefotetan induced anaphylaxis with negative pre-skin test, used for surgical prophylaxis. A 82-year-old female was scheduled for total knee replacement therapy. She had no previous history of allergy and her skin test results were also negative. On her right knee surgery, she underwent cefotetan therapy as a surgical prophylaxis for a week with no problems identified. Next left knee surgery, she also received the prophylaxis of intravenous cefotetan. However, a few minutes later, anaphylactic reaction developed with vomiting, severe hypotension, bronchospasm, and dyspnea. After immediate intensive care treatment, she recovered without significant complications. Though commonly used laboratory data in case reports, such as the specific IgE, tryptase, histamine, or allergic skin prick test were limited, we successfully confirmed anaphylaxis based on clinical criteria for diagnosing anaphylaxis based on WAO 2011 guideline with through concurrent patient°Øs medical history review and the process of identifying the causes.

• The Journal of Internal Korean Medicine
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• v.26 no.1
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• pp.119-128
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• 2005

모든 알레르기 반응의 중심축이 되는 비만세포는 주로 피부, 위장관 및 호흡기관의 점막에 분포하고 있다. 활성화된 비만세포는 즉각형 알레르기 반응을 일으키는 여러 인자들을 방출시키게 된다. 方藥合編(방약합편)에 따르면 蔘蘇飮(삼소음)은 알레르기 鼻炎(비염), 發熱(발열), 風寒(풍한), 頭痛(두통), 기침에 效能(효능)이 있는 處方(처방)이다. 본 硏究(연구)는 蔘蘇飮(삼소음)의 肥滿細胞(비만세포) 의존성 아나필락시 반응(anaphylactic reaction)에 대한 藥理(약리) 效果(효과)를 조사하기 위한 것이다. 蔘蘇飮(삼소음)은 compound 48/80으로 유발되는 전신성 아나필락시 쇼크(systemic anaphylactic shock)와 耳介(이개) 浮腫(부종) 反應(반응)(ear swelling response)을 농도 의존적으로 억제하였다. 蔘蘇飮(삼소음)을 0.1, 1 mg/ml로 전처리 하였을 때, 흰쥐 복강 肥滿細胞(비만세포)(rat peritoneal mast cells, RPMCs)에서 compound 40/80에 의해 유발되는 히스타민 분비는 감소하는 것으로 나타났다. 또한 蔘蘇飮(삼소음)은 anti-dinitrophenyl IgE에 의해 활성화 된 수동 피부 아나필락시(passive cutaneous anaphylaxis, PCA)를 농도 의존적으로 抑制(억제)하였다. 결론적으로 蔘蘇飮(삼소음)은 肥滿細胞(비만세포) 의존성 즉각형 알레르기 反應(반응)을 抑制(억제)하여, 항 아나필락시 활성(anti-anaphylactic activity)을 가지는 것으로 보여 진다.

• Applied Microscopy
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• v.40 no.4
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• pp.201-209
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• 2010

Korean traditional herbs, especially Anemarrhena asphodeloides (A. asphodeloides), Salvia miltiorrhiza (S. miltiorrhiza), and Terminalia chebula (T. chebula) have been known to have the immunomodulatory, anti-tumor, and anti-inflammatory effects. However, direct cellular mechanism underlying the mast cell-mediated anaphylaxis-like reaction has poorly been understood. In the present study, the effects of the methanol extracts of A. asphodeloides (MEAA), S. miltiorrhiza (MESM), and T. chebula (METC) on anaphylactic reaction were investigated. Using in vitro and in vivo experiments, the influences of MEAA, MESM, and METC on the compound 48/80-induced anaphylaxis-like reaction and mast cell activation, and IgEmediated PCA were examined. Results are below; 1) MEAA, MESM, and METC significantly inhibited systemic anaphylaxis-like reaction, ear swelling response, and IgE-mediated PCA. 2) the compound 48/80-induced mast cell degranulation, histamine release of rat peritoneal mast cells (RPMC) were significantly inhibited by the pretreatment with MEAA, MESM, and METC, and 3) the compound 48/80-induced calcium influx in RPMC was significantly inhibited by the pretreatment with MEAA, MESM, and METC. These results suggest that MEAA, MESM, and METC may be an activity to inhibit the compound 48/80-induced or anti-DNP IgE-mediated anaphylactic reactions by blocking histamine release and calcium uptake into RPMC. MEAA, MESM, and METC potentially may serve as an effective therapeutic tool for allergic diseases.