• Development and Reproduction
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• v.20 no.2
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• pp.65-79
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• 2016

We observed the osteological development of larval and juvenile red spotted grouper (Epinephelus akaara) in order to generate data for the assessment of skeletal deformities and to inform phylogenetic systematics research. Larvae and juveniles were obtained from a aquafarm in Muan-gun, Jeolla-namdo Province, Korea. The average water temperature at the time of breeding was $23.0^{\circ}C$ and average water salinity was 33.0 psu. Freshly hatched fish larvae had not undergone any ossification, but ossification of the parasphenoid bone, which forms the base of the cranium, occurred as the juveniles reached an average body length (BL) of 2.49 mm. At the same time, ossification of the preopercle and opercle occurred in the operculum, and ossification of the maxilla, which forms the upper jaw, and the dentary bones, which form the lower jaw, began. In addition, ossification of the vertebra occurred by formation of 7 vertebral centra and the neural spine in the abdominal vertebra. When the juveniles reached an average (BL) of 5.22 mm, ossification of the nasal, lateral ethmoid, and alisphenoid bones occurred in the cranium; ossification of the endopterygoid and metapterygoid bones began in the palatine region; and ossification of the hypohyal and interhyal bones occurred in the hyoid arch. At an average (BL) of 20.9 mm, ossification of the basisphenoid bone in the cranium and the suborbital bone in the orbital region occurred. Ossification of the vertebra then occurred by the formation of long pairs of ribs from the third to the ninth abdominal vertebrae, completing osteological development.

• Animal Systematics, Evolution and Diversity
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• v.28 no.4
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• pp.279-290
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• 2012

Plecoglossus altivelis (ayu) is an amphidromous fish widely distributed in Northeastern Asia from the East China Sea to the northern Japanese coastal waters, encompassing the Korean Peninsula within its range. The shore lines of northeastern region in Asia have severely fluctuated following glaciations in the Quaternary. In the present study, we investigate the population genetic structure and historical demographic change of P. altivelis at a population level in East Asia. Analysis of molecular variance (AMOVA) based on 244 mitochondrial control region DNA sequences clearly showed that as the sampling scope extended to a larger geographic area, genetic differentiation began to become significant, particularly among Northeastern populations. A series of hierarchical AMOVA could detect the genetic relationship of three closely located islands between Korea and Japan that might have been tightly connected by the regional Tsushima current. Neutrality and mismatch distribution analyses revealed a strong signature of a recent population expansion of P. altivelis in East Asia, estimated at 126 to 391 thousand years ago during the late Pleistocene. Therefore it suggests that the present population of P. altivelis traces back to its approximate demographic change long before the last glacial maximum. This contrasts our a priori expectation that the most recent glacial event might have the most crucial effect on the present day demography of marine organisms through bottleneck and subsequent increase of effective population size in this region.

• Korean Journal of Microbiology
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• v.35 no.3
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• pp.173-179
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• 1999

As a result of PCR-RFLP, the isolates used in this study were classified into five groups. Isolates 1 and 3 were included in AG-5 with 97% genetic similarity. Isolates 12 and 13 were included in AG-1 wilh 100% genetic similarily. Isolates 10 and AG-2-2 showed 97% similarity Isolates 7, 8, 11. 13, and 15 were included in AG-1. When isolates of 4, 5, 7 and 8 were restricted with Hae I. there was a single 700 bp fragment matched with AG-1. A 517 bp restriction fragment of isolate 9 was matched with AG-2-1. Based on the result of southem hybridization of genomic DNAs, all isolates restricted with Msp I showed more variable restriction differences than those restricted with Hae Ill. Isolates AG-2-1 and 9 showed 200 bp restriction fragment, and isolates 3 and AG-1 showed 1 kb restriction fragments.

• Animal Systematics, Evolution and Diversity
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• v.29 no.2
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• pp.136-143
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• 2013

The collared scops owl that occurs in Korea is a protected species but its exact specific status has been questioned. To resolve the species status, a molecular phylogenetic analysis was conducted using two fragments of mitochondrial DNA, cytochrome b (cyt b, 891 bp) and NADH dehydrogenase subunit 2 (ND2, 627 bp) genes. Phylogenetic trees of cyt b revealed that all Korean specimens formed a monophyletic group with Japanese scops owl Otus semitorques with very low sequence divergence (d=0.008). We obtained a similar ND2 tree as well (d=0.003); however, the genetic distance between Korean individuals and O. lempiji from GenBank (AJ004026-7, EU348987, and EU601036) was very high and sufficient enough to separate them as species (cyt b, d=0.118; ND2, d=0.113). We also found that Korean species showed high differentiation from O. bakkamoena (AJ004018-20 and EU601034; cyt b, d=0.106; ND2, d=0.113) and O. lettia (EU601109 and EU601033, cyt b, d=0.110; ND2, d=0.117) as well. Therefore, we suggest that the Korean collared scops owl should be designated as Otus semitorques.

• Animal cells and systems
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• v.4 no.1
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• pp.1-7
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• 2000

The genus Dendronephthya, generally known as soft corals, is reported as an abundant and variable taxon. They mostly distribute in warmer waters of the Undo-Pacific Ocean region including Korea. In spite of their abundance and ecological importance as habitats of marine organisms, there are difficulties in the study of their identification and systematics because they have morphological variabilities and limited taxonomec characters. To resolve the problems, we attempted to elucidate the genetic relationships in the genus Dendronephthya by using random amplified polymorphic DNA (RAPD) analysis. This study was based on eight dendronephthian species and one Alcyoniidae species, Alcyonium gracillimum, as an outgroup. The results from all analysis suggest that they could be classified into four groups by the growth form and the anthocodial grades as follows: the first one,D. putteri and D. suensoni with the divaricate form and VI grade; the second one,D. sp.1 and D. sp.2 with the divaricate form and III or IV grade; the third one, D. gigantea and D. aurea being closer than D. spinifera with the glomerate form and III grade; the last one, D. castanea related to D. gigantea rather than D. putteri with the umbellate form and IV grade. Moreover, the divaricate form was separated from the group of the glomerate and umbellate form. At the intraspecies level, the types of the D. castanea, D. gigantea and D. spinifera were separated depending on the feature of spicules in the polyp head, and the coloration could not influence genetic variation. From this study, we can confirm that their morphological characters are compatible with the genetic variation, also RAPD analysis is a very useful method for resolving the systematic relationships of den-deonephthians.

• Animal Systematics, Evolution and Diversity
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• v.18 no.1
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• pp.75-84
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• 2002

MtDNA cyt b gene was used to investigate the geographic variation of 11 populations (106 individuals) of the planktonic developing, periwinkle Littorina brevicula, throughout Eastern, Western, and Southern coastal regions in Korea. The sequence of 500 base pairs and 13 different haplotypes were determined. Haplotype LbA was predominated through the populations studied with frequence of 0.877. Haplotypes were shown different frequencies in each coastal region (0.82, 0.90, and 1.00, respectively). enetic analysis of the 61 individuals of L. brevicula from the polluted and unpolluted sites yielded 8 distinct haplotypes. Haplotype LbA also was most common, and it was shared by 0.872 of frequency among specimens.

• Animal Systematics, Evolution and Diversity
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• v.2 no.2
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• pp.67-78
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• 1986

한국해역에서 알려진 집게류는 12과 57종, 게류는 20과 183종이다. 집게류는 집게과 (Paguridae) (19종)와 넓적왼손집게 과 (Diogenidae) (12종) 의 종들이 많고 게류에서는 물맞이게 과 (Maidae) (33종) 의 종이 가장 asg고 다음이 바위게 과(Grapsidae) (29종), 부채게 과 (Xanthidae) (22종), 꽃게 과 (Portunidae) (16종) , 달랑게 과(Ocypodidae) (16종)의 순이다. 저자는 한국 해역을 동해, 대한해협, 제주도해역, 황해의 4구역으로 나누고, 집게류와 게류를 북방형, 온대형, 남방형의 3분지형으로 나누었다. 집게류의 경우 북방형 9종, 온대형 40종, 남방형 8종이고, 게류의 경우 북방형 7종, 온대형 87종, 남방형 89종이다. 집게류의 경우 동행 29종, 대한해협에 30종, 제주도해역에 30종, 황해에 21종이 게류의 경우 위의 순서대로 62종, 121 종, 104 종, 74종이 분포하며, 각 해역에서의 분포형도 고찰했다. 각 해역에만 분포하는 종의 수는 집게의 경우 동해에 8종, 대한해협에 3종, 제주도해역에 16종, 황해에 0종, 게류의 경우 위의 순서로 8종, 20종 40종, 12종이다. 집게류는 북방형종이, 게류는 남방형종이 비교적 많으며 동해에는 북방형종들이 제주도에는 남방형종들이 비교적 많다. 4구역 중 2 구역 (6조합) 사이의 유사계수(r=a/a+b+c)를 계산한 바 양 분류군 모두 대한 해협과 황해 사이의 유사성이 가장 크고 (집게류 ; 0.55, 게류 : 0.59), 제주도해역과 황해 사이의 유사성이 가장 적다. (집게류 ; 0.20, 게류 : 0.19). 분포상을 이해하기 위하여 각 해역의 환경조건도 알아보았는바, 분포의 양상과 해황과 잘 부합된다.

• Animal Systematics, Evolution and Diversity
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• v.12 no.4
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• pp.331-347
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• 1996

The geographic distribution and variation for Rhinogobius brunneus were surveyed by means of allozymic and morphological analyses and it was revealed that the Korean populations of R. brunneus comprise three distinct types, Type-A, Type-B, and Type-C, which show considerable differentiation to a degree of interspecific level(Rogers' S(1972): $S_{A-B}$=0.631, $S_{A-C}$=0.628, $S_{B-C}$=0.661). In addition, no evidence of gene flow among the types was found at sympatric area and it is assumed that reproductive isolation is completed. Moreover there is microhabitat segregation according to the distance from river mouth among each types and which segregation was regarded as a factor to facilitate reproductive isolating mechanism. Therefore, based on the evidence presented above, these three types of R. brunneus are considered as typical discrete species.

• Korean Journal of Plant Taxonomy
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• v.39 no.2
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• pp.89-99
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• 2009

We used Maximum parsimony and Neighbor-joining to investigate phylogenetic relationship of the genus Thalictrum in Korea with 39 morphological characters. The plant materials were used for ingroup 21 taxa and outgroup 1 taxon including a Korean endemic plant. This study confirmed that Sect. Thalictrum and Sect. Camptonotum formed monophyletic group by 100% and 83% bootstrap values respectively, and Sect. Camptonotum appeared to be Sect. Thalictrum's sister group. Sect. Erythrandra and Sect. Physocarpum formed paraphyletic groups. Sect. Physocarpum except T. osmorhizoides appeared to be polytomy. Sect. Tripterium and Sect. Omalophysa formed an independent clade. The shape of stamen is the important character representing the phylogenetic pattern of the genus Thalictrum; the bat and filiform types might be derived from clavate type. The pollination patterns (entomophily, anemophily), and presence of calyx at flowering time may also be related to the evolution of the Thalictrum flowers.