• KSBB Journal
• /
• v.22 no.4
• /
• pp.179-184
• /
• 2007

This study introduces the characteristics and some applications of repetitive polypeptides, especially to the biomaterial, tissue engineering scaffolds, drug delivery system, and DNA separation systems. Since some fibrous proteins, which consist of repeating peptide monomers, have been reported that their physical properties are changed dramatically by means of temperature alteration or pH shifting. For that reason, fibrous protein-mimetic polypeptides, which are produced by the recombinant technology, can be applied to the diverse biological fields. Repetitive polypeptides can also be used in the bioseparation area such as DNA sequencing, because they make DNA separation possible in free-solution electrophoresis by conjugating DNA fragments to them. Moreover, artificial synthesis of repetitive polypeptides helps to demonstrate the correlations between mechanical properties and structures of natural protein polymer, which have been proven that repetitive domains are affected by the sequence of the repeating domains and the number of repeating subunits. Repetitive polypeptides can be biologically synthesized using some special cloning methods, which are represented here. Recursive directional ligation (RDL) and controlled cloning method (CCM) have been proposed as excellent cloning methods in that we can control the number of repetition in the multimerization of polypeptides and the components of repetitive polypeptides by either method.

• Journal of Hydrogen and New Energy
• /
• v.22 no.6
• /
• pp.759-764
• /
• 2011

Mixed-conducting oxide powders, $BaCe_{0.9}Y_{0.1}O_{2.95}$ (BCY) and $SrCe_{0.9}Y_{0.1}O_{2.95}$ (SCY) powders have been prepared by a solid-state reaction method. Xray diffraction patterns of the prepared powders showed the sharp peaks of the $BaCe_{0.9}Y_{0.1}O_{2.95}$ and $SrCe_{0.9}Y_{0.1}O_{2.95}$ phases. The oxide powders that were prepared by attrition milling showed rather large particles and severe necking between particles in FE-SEM images as well as residual reactant ($BaCO_3$) and secondary phases ($SrCeO_3$ and $CeO_2$) in XRD patterns. The oxide powders prepared using ball milling showed particles under approximately 500 nm and typical XRD patterns of the $BaCe_{0.9}Y_{0.1}O_{2.95}$ and $SrCe_{0.9}Y_{0.1}O_{2.95}$ phases. Ceramic membranes of the $BaCe_{0.9}Y_{0.1}O_{2.95}$ and $SrCe_{0.9}Y_{0.1}O_{2.95}$ phases were fabricated by the aerosol deposition method using the oxide powders synthesized.

• Membrane Journal
• /
• v.22 no.1
• /
• pp.62-71
• /
• 2012

In this study, for preparation of gas separation membrane with high permeability, hydroxy polyimide was synthesized using 6FDA and APAF. Synthesis of HPI was confirmed by H-NMR and FT-IR, thermal property of membrane was characterized by Differential scanning calorimetric (DSC) and thermogravimetric analyzer (TGA). Especially, the synthesized HPI can possible to search conversion to PBO at $450^{\circ}C$. To obtain the membrane having high permeability, ternary system consist of polymer, solvent and non-solvent additive was introduced, asymmetric HPI flat sheet membrane was prepared by phase inversion method. Finally, the change of morphology with each component was observed through FE-SEM.

• The Journal of the Acoustical Society of Korea
• /
• v.38 no.2
• /
• pp.177-183
• /
• 2019

In this paper, we proposed an application method of ICA (Independent Component Analysis) to passive line array sonar to separate interferences from target signals in low frequency band and compared performance of three conventional ICA algorithms. Since the low frequency signals are received through larger bearing angles than other frequency bands, neighboring beam signals can be used to perform ICA as measurement signals of the ICA. We use three ICA algorithms such as Fast ICA, NNMF (Non-negative Matrix Factorization) and JADE (Joint Approximation Diagonalization of Eigen-matrices). Through experiments on real data obtained from passive line array sonar, it is verified that the interference can be separable from target signals by the suggested method and the JADE algorithm shows the best separation performance among the three algorithms.

• Journal of the Korean Crystal Growth and Crystal Technology
• /
• v.13 no.3
• /
• pp.145-151
• /
• 2003

Using computational fluid dynamics (CFD) method, we investigated three-dimensional fluid flow field and particle movement with respect to the injected gas flow rate variation in typical cyclone separator system. The results of numerical investigation were deduced by coupling the analysis of fluid flow field with Wavier-stokes equation and the tracking of the particle trajectory with Langrangian approach. It was shown that the increasing of injected gas flow rate resulted in the increasing of pressure loss in the separator. This change of inner pressure had an effect on an aspect of the fluid flow in the separator. Particle movement was determined by fluid flow in the separator and was fully depended on a diameter of particles under the fixed flow rate. Increasing of injected gas flow rate was led to an increasing of the trace of particle, so the particles moved to the lower part of the separator. For this reason, the minimum diameters of the particles were decreased and increased the separation rate under the fixed particle diameter. In conclusion, the changes of injected gas flow rate have an important factor to the fluctuation of the fluid flow field and particle trajectory in the separator.

• Korean Journal of Food Science and Technology
• /
• v.22 no.1
• /
• pp.56-60
• /
• 1990

The changes in the partition coefficient of model proteins (lysozyme, myoglobin, conalbumin, bovine serum albumin) in an aqueous two-phase system formed by polyethylene glycol and dextran were examined in order to improve the capacity of counter current distribution for the protein fractionation and concentration. The protein distribution patterns in CCD with 30 tubes varied with the pH of the system, and both theoretical and measured values agreed well. From the mixture of model protein, pure BSA fraction was appeared at the upper-phase of 14th tube having pH 4.5, pure myoglobin at the lower-phase of the 16th be with pH 6.5 and conalbumin at the lower-phase of 4th tube with pH 12. The result indicated the possible use of CCD method for protein fractionation, if the partition coefficient of proteins was manipulated by pH and other means.

• Journal of Climate Change Research
• /
• v.7 no.3
• /
• pp.249-256
• /
• 2016

Porous $Al_2O_3$ hollow fiber membranes were successfully prepared by dry-wet spinning/sintering method. The SEM image shows that the $Al_2O_3$ hollow fiber membrane consists mostly of sponge pore structure. The contact angle and the breakthrough pressure were $126^{\circ}$ and 1.91 bar, respectively. This results indicate that the $Al_2O_3$ hollow fiber membranes were successfully modified to hydrophobic surface. The hydrophobic modified $Al_2O_3$ hollow fiber membranes were assembled into a membrane contactor system to separate $CO_2$ from a model gas mixture of the flue gas at elevated gas velocity. The $CO_2$ absorption flux was enhanced when the gas velocity increased from $1{\times}10^{-3}$ to $6{\times}10^{-3}$ m/s. Whereas the $CO_2$ absorption flux was decreased with the number of hollow fiber membrane of a module because of the concentration polarization. Furthermore, we developed an lab-scale $Al_2O_3$ hollow fiber membrane contactor modules and their system (i.e., $CO_2$ absorption using the $Al_2O_3$ membrane and monoethanolamine (MEA)) that could dispose of over $0.02Nm^3/h$ mixture gas (15% $CO_2$) with the removal efficiency higher than 95%. The results can be useful in a field of the membrane contactor for $CO_2$ separation, helping to design and extend a equipment.

• Korean Journal of Veterinary Research
• /
• v.27 no.2
• /
• pp.191-200
• /
• 1987

Attempts were to produce porcine leukocyte interferon(PorLeIF) and porcine immune interferon (PorIIF) in the culture of porcine leukocytes. The interferons produced were tested for antiviral activity against vesicular stomatitis virus on poreine-derived PK(15) cells, human-derived FL cells, and Korean native black goat-derived BGK cells. The results were summarized as follws: 1. In the isolation of porcine leukocytes, the mean isolation rate by the buffy coat separation method (28.7%) was higher than that by the hydroxyethyl starch-RBC sedimentation method (9.2%). 2. When NDV(BI)-induced PorLeIFs were assyed on PK(15) cells and FL cells, the mean titers were 129 IU/ml and 72 IU/ml respectively, being 55.8% of the activity in homologous species system expressed in heterologous system. 3. The activities of PHA P-induced PorIIFs were 197 IU/ml on PK (15) cells and no activity on human FL cells. The mean antiviral activity of PorIIF was 1.5 times that of PorLeIF in PK (15) cells. 4. The cytopathic effect of vesicular stomatitis virus was observed in BGK cells derived from Korean native black goat kidney permitting interferon assay on the cells. While the cross-species antiviral activity of reference human ${\alpha},\;{\beta}-interferon$ was observed on the cells, PorLeIF and PorIIF did not show any activity.

• Journal of the Korean Institute of Intelligent Systems
• /
• v.10 no.5
• /
• pp.417-422
• /
• 2000

In this paper, an efficient fuzzy rule generation scheme for adaptive neuro-fuzzy system modeling using the Independent Component Analysis(ICA) as a preprocessing is proposed. Correlation between inputs was not considered in the conventional neuro- fuzzy modeling schemes, such that enormous number of rules and large amount of error were unavoidable. The correlation between inputs is weakened by employing ICA so that the number of rules and the amount of error are reduced. In simulation, the Box-Jenkins furnace data is used to verify the effectiveness of the proposed method.

• Journal of the Korean Society of Combustion
• /
• v.21 no.3
• /
• pp.7-15
• /
• 2016

The fuel in conventional liquid fuel combustor is atomized by spray method for high efficiency and low emissions. To improve the overall fuel efficiency and lower pollutant emissions in liquid fuel combustion systems, the effective spatial and temporal separation of droplet evaporation from normal spray process is needed. In this paper, the recuperation of high temperature burnt gas for fuel evaporation was proposed to develop a cylindrical premixed combustor. The recuperation process using U shaped tube is effective to evaporate the liquid fuel. The results show that the flame mode is changed into red radiation flame, blue flame and lift off flame with decreasing equivalence ratio as gas fuel combustion mode. In particular, the blue flame is found to be very stable at heating load 9.2 kW and equivalence ratio 0.731. NOx was measured blow 105 ppm ($O_2$ zero base) from equivalence ratio 0.705 to 0.835. CO which is a very important emission index in liquid fuel combustor was observed below 5 ppm ($O_2$ zero base) under the same equivalence region.