• Biomolecules & Therapeutics
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• v.12 no.4
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• pp.242-249
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• 2004

The main aim of this study was to investigate stress related activities of Sun-ginseng extract as a candidate for anti-stress-related functional supplement by comparing its effect to those of red ginseng, which is also known to alleviate stress. Normal group was not exposed to stress while the control group was exposed to stress. Rats were orally administered once a day with 200 mg red ginseng (RG) extract, 100 or 200 mg Sun-ginseng (SG) extract/kg body weight. Mice were given water containing 400 mg red ginseng extract, 200 or 400 mg SG/100 mL potable water. Rats were given supplements for 5 days without stress, and 5 days with restraint and electroshock stress. After final stress, stress-related behavioral changes of experimental animals were recorded and levels of blood corticosterone were measured. Mice were given supplements for 5 days through drinking water, and then fatigue related motor activity were recorded. SG-supplementation partially blocked stress effect on locomotion and elevated plus maze test in rats, and also partially blocked stress-induced behavioral changes such as freezing, burrowing, smelling, facewashing, grooming and rearing behavior in rats. SG-supplementation decreased blood corticosterone level which is increased by stress in rats. Effects of SG may not be modulated by GABAnergic nervous system. SG-supplementation prolonged swimming time and staying time on the wire and rotarod wheel in mice. These results suggest that SG partially protects living organisms from stress attack in some cases and thus has the potential to be used as a functional food to alleviate stress response.

• The Journal of Korean Physical Therapy
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• v.13 no.2
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• pp.281-292
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• 2001

This study was performed to investigate the effect of therapeutic exercise on brain-derived neurotrophic factor manifestation after global brain ischemia in rats. Nine rats with global ischemia were divided at random into two group. In the control group, three rats remained in cage. But, in the end, two rats were alive. In the therapeutic exercise group, six rats remained. The five rats of this group was swam for 30 minutes everyday for a week. The brain-derived neurotrophic factor expression was identified from immunohistochemistry. The results of this study were as follows : 1. In the control group, a little expression of brain-derived neurotrophic factor was observed at cortex and hippocampus layer, but cell body and axon was observed obscurely. 2. In the experimental group, a much expression of brain-derived neurotrophic factor was observed at cortex and hippocampus layer, and cell body and axon was observed clearly. In the neurological examination(beam-walking test). experimental group was obtained higher 1.4 points than control group. BDNF expression was increased by swimming for 30 minutes everyday for a week. Therefore, therapeutic exercise contribute to brain plasticity after brain ischemia.

• Korean Journal of Human Ecology
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• v.15 no.4
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• pp.527-539
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• 2006

The purpose of this study was to investigate the satisfaction and current conditions of playground environment in kindergartens and child care centers in Daejeon. Data were collected from 112 directors of kindergartens and child care centers through a structured questionnaire. Data were analyzed by using of frequency, percentage, mean, reliability analysis, crosstab, independent sample t-test, multiple regression. The major results showed the following. 1) 89.3% of subjects(100.0% of kindergartens and 79.7% of child care centers) have a playground. Most of playgrounds were located in the front yard. The playground's play equipments/areas were usually composed of slides, sand play areas, benches, empty playing fields, swings. Flowers/plants, shrubs were most of the natural factors of the playgrounds. 2) Slides and swimming pools of play equipments/areas were rated highest on the satisfaction level. Grass, flowers/plants, and shrubs as the natural factors of the playgrounds, were rated highest on the satisfaction level. 3) The playground was divided into 5 categories, which were made of manufactured play equipment settings, manipulative settings, rest settings, natural settings, adventure settings. Manufactured play equipment settings were rated highest on the satisfaction level. 4) Among 5 categories of the playground's play area, manufactured play equipment settings showed valid variables to playground's satisfaction level.

• Proceedings of the Korea Contents Association Conference
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• 2015.05a
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• pp.9-10
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• 2015

Purpose : The purpose of this study was to report the incidence of drowning accident in order to prevent and decrease the accidents of drowning. Methods : Data were obtained on all accidents of drowning accidents occurred were identified by using the Emergency Activity Daily Report Data from the National Emergency Management Agency (NEMA). Results : The age, location, days, season of the drowning accident were examined. The Chi-square test was used to compare each group. All comparisons were made at the p<0.05 level of significance. The result show that the age of drowning accidents for victims aged 20-29 was 27.9%, and for victims aged 30-39 years, 18.7%. In reguard to the age group, drowning accidents was age 20-39; 51(44.8%). In reguard to the locations, the number of drowning accidents in a lake or reservoir was 62(53.4%). The number of drowning accidents in a lake or reservoir during etc was 51(58%). In reguard to the days, the number of drowning accidents on Saturday was 30(25.8%), on Sunday 32(27.6%). The weather of drowning accidents was sunny 60(51.8%), rainy 40(34.4%), cloudy 16(13.8%). The season of drowning accidents was summer 53(45.7%). In reguard to season, two groups are significantly different. Conclusion : Drowning is a leading cause of injury related accidents. Understanding the characteristics of drowning is the first step to developing prevention strategies that may be beneficial for people of all ages and under nearly all circumstances are increased swimming ability, lifeguard staffing, providing life jackets, and public education activities.

• Nutritional Sciences
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• v.8 no.2
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• pp.118-124
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• 2005

To examine the eating attitudes, weight concerns, dietary intake, and menstrual function of Korean female elite athletes, 109 subjects in seven sports disciplines (rhythmic gymnastics, synchronized swimming, badminton, volleyball, Taekwondo, field hockey, and soccer) responded to a questionnaire consisted of three parts; eating attitudes and behavior(Eating Attitude Test-26; EAT26), body weight perception (Body Dissatisfaction Index: BDI) and weight control behavior, and menstrual history and status. They also recorded three-day dietary intake. Body weight (43.6$\pm$4.3 kg) and body mass index (16.7$\pm$1.4 kg/$m^2$) in rhythmic gymnasts were lower than those in other athletes (P<0.05). EAT26 scores were not different among sport events (12.3$\pm$6.5 total), however, eating disturbances (EAT26 score$\geq$20) were highly prevalent in aesthetic athletes ($30\%$) than in others ($5\%$). More than half of the athletes perceived themselves overweight and four fifth of the athletes desired to reduce weight about 4.4 kg. The gymnasts consumed the lowest caloric intake (1028:t371) while the volleyball players did the highest (2995$\pm$342 kcal/d) (P<0.05). The BDI score was not different among sport events. Three fourth of the athletes experienced weight control, and the most frequently used weight reduction method was exercise followed by using robber suits, diet, and sauna. About $40\%$ of the subjects reported irregular menstrual cycles, but menstrual dysfunction ($\leq$6 menses/yr) was only $5\%$. Generally, the Korean female elite athletes desired to reduce weight from their current body weight. No differences in eating attitude and body dissatisfaction were noticed among athletic disciplines. However, eating disturbances were highly prevalent in aesthetic athletes who also reported much less energy intake than the recommend daily energy intake. It appeared that weight reduction methods were not properly practiced in these population. Menstrual dysfunction was minimally reported.

• Journal of Microbiology and Biotechnology
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• v.21 no.10
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• pp.1088-1096
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• 2011

The purpose of this study was to evaluate whether wild ginseng (WG) administration could attenuate anxiety- and depression-like behaviors and expression of corticotrophin-releasing factor (CRF) and neuropeptide Y (NPY) following withdrawal from repeated morphine administration in rats. Male rats were administered daily doses of WG (50, 100, or 200 mg/kg, i.p.) for 5 days, 30 min before morphine injection (40 mg/kg, s.c). The anxiety- and depression-like behavioral responses were measured 72 h after the last morphine injection using an elevated plus maze (EPM) and forced swimming test (FST), respectively. Changes in hypothalamic CRF and NPY expressions were also examined by analyzing their immunoreactivities in the hypothalamus. Daily administration of WG significantly reduced anxiety- and depression-like behavior, and elicited the suppression of CRF expression and the stimulation of NPY expression in the hypothalamus. Our results demonstrated that WG extract might be effective at inhibiting the anxiety and depression responses due to morphine withdrawal by possibly modulating the hypothalamus CRF and NPY systems. Furthermore, these findings imply that WG extract can be used for developing new medication to cure or alleviate morphine withdrawal symptoms and to prevent relapses of morphine use.

• Korean Journal of Pharmacognosy
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• v.51 no.1
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• pp.70-77
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• 2020

Mentha arvensis is used traditional medicine to treat various disorders. In the present study, M. arvensis were extracted by the solid-phase microextraction (SPME) method and analyzed by gas chromatograph-mass spectrometry (GC-MS). We investigated the protective effects and mechanisms of a M. arvensis extract on scopolamine-induced cognitive and memory impairment. Mice were orally pretreated with a M. arvensis extract or normal saline, and then behavior tests were conducted 30 min after scopolamine injection. The antioxidant capacities were analyzed by free radical scavenging (DPPH and ABTS). Acetylcholinesterase (AChE) activity were also measured using Ellman's method ex vivo test. In behavior tests, percent of spontaneous alteration, escape latency and swimming time in target quadrant were improved by the administration of the M. arvensis extract, which suggests that the M. arvensis extract improves memory function in the scopolamine-treated mice model. In addition, M. arvensis extract showed inhibition of the free radical and AChE activity. The results of the present study suggest that the M. arvensis extract ameliorates scopolamine-induced cognitive and memory deficits through the inhibition of free radicals and AChE activity. Therefore, M. arvensis may be a promising neuroprotective agent for management of learning and memory improvements in human dementia patients.

• The Plant Pathology Journal
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• v.36 no.4
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• pp.346-354
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• 2020

Pectobacterium, which causes soft rot disease, is divided into 18 species based on the current classification. A total of 225 Pectobacterium strains were isolated from 10 main cultivation regions of potato (Solanum tuberosum), napa cabbage (Brassica rapa subsp. pekinensis), and radish (Raphanus sativus) in South Korea; 202 isolates (90%) were from potato, 18 from napa cabbage, and five from radish. Strains were identified using the Biolog test and phylogenetic analysis. The pathogenicity and swimming motility were tested at four different temperatures. Pectolytic activity and plant cell-wall degrading enzyme (PCWDE) activity were evaluated for six species (P. carotovorum subsp. carotovorum, Pcc; P. odoriferum, Pod; P. brasiliense, Pbr; P. versatile, Pve; P. polaris, Ppo; P. parmentieri, Ppa). Pod, Pcc, Pbr, and Pve were the most prevalent species. Although P. atrosepticum is a widespread pathogen in other countries, it was not found here. This is the first report of Ppo, Ppa, and Pve in South Korea. Pectobacterium species showed stronger activity at 28℃ and 32℃ than at 24℃, and showed weak activity at 37℃. Pectolytic activity decreased with increasing temperature. Activity of pectate lyase was not significantly affected by temperature. Activity of protease, cellulase, and polygalacturonase decreased with increasing temperature. The inability of isolated Pectobacterium to soften host tissues at 37℃ may be a consequence of decreased motility and PCWDE activity. These data suggest that future increases in temperature as a result of climate change may affect the population dynamics of Pectobacterium.

• Journal of Oriental Neuropsychiatry
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• v.15 no.2
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• pp.89-101
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• 2004

Objective : The present experiments were designed to study on the immune-enhancing effect of Mountain grown ginseng, Mountain cultivated ginseng, and Panax ginseng Method : In order to compare the immune-enhancing effect of moutain grown ginseng, moutain cultivated ginseng and Panax ginseng, the study was done through the forced swimming test (FST), measurement of T helper Th1, Th2 cytokines and fatigue related factors. Result : Moutain grown ginseng and panax ginseng decreased the immobility time in the FST compared to the control. Glucose, blood urea nitrogen (BUN), creatinine, lactate dehydrogenase (LDH) and Total-protein (T-protein) in serum were investigated. The serum achieved from ginseng administered mouse showed higher BUN, T-protein than the control. moutain grown ginseng administered group showed lower LDH than the control group. moutain grown ginseng administered mouse showed higher glucose than the control. Creatinine was same in either experimental or control group. Ginseng-induced cytokine production in human T-cell line, MOLT-4 cells and mouse peritoneal macrophages were compared. Moutain cultivated ginseng (10-4 dilution) and panax ginseng (10-3 dilution) were increased the interferon $IFN-{\gamma}$ production compared with media control (about 1.6-fold P<0.05) at 48 h. Moutain grown ginseng (10-4 dilution) was increased the $IFN-{\gamma}$ and interleukin IL-4 production compared with media control (about l.4-fold for $IFN-{\gamma}$ and 1.6-fold for IL-4 P<0.05) at 48 h. Moutain grown ginseng (10-3 dilution) and moutain cultivated ginseng (10-4 dilution) were increased the turmor necrosis factor $TNF-{\alpha}$ production compared with $rIFN-{\gamma}$ treated cells (about 1.9-fold for $TNF-{\alpha}$ P<0.05), respectively. Moutain cultivated ginseng (10-3 dilution) was increased the IL-12 production compared with $rIFN-{\gamma}$ treated cell (about 1.7-fold for IL-12 P<0.05). Conclusion : These data suggest that three different three kinds of ginseng act on immune responses in different aspects.

• Journal of the Korean Society of Food Science and Nutrition
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• v.32 no.7
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• pp.1126-1131
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• 2003

In this study, we investigated the effects of detoxified puffer liver (PL) oil on fatigue, hepatotoxicity and hyperlipidemia. There are no toxicities in both raw and purified PL oil. The test of swimming time was extended in detoxified PL oil pretreated group compared to the non-treated group. When rats treated with PL oil, the hepatic injuries induced by carbon tetrachloride or DL-galactosamine were reduced. The increased serum triglyceride and total cholesterol by poloxamer-407 were lowered by treating with PL oil remarkably. Also the bleeding time of hyperlipidemic animals was extended and plasma clotting time was delayed by PL oil.