• 대한수의학회지
• /
• 제33권4호
• /
• pp.623-632
• /
• 1993

This study was conducted to develop a sensitized latex-antigen for serodiagnosis of toxoplasmosis in animals. Tachyzoites of T gondii(RH-strain) harvested from mouse peritoneal cavity were purified through the filtraton of polycarbonate membrane(pore size, $3.0{{\mu}m}$, Costar Co.) and disrupted by ultrasonicator. The tachyzoite suspension was ultracentrifuged for 30 min at $60,000{\times}g(4{^{\circ}C})$ and the supernatant was used as a water-lysate antigen. Polystyrene latex particles of $0.8{{\mu}m}$ in diameter(Sigma) were used for the preparation of sensitized latex-antigen suspension. The several parameters including the preparation conditions, incubation buffer. serum dilution buffer and stability of agglutination reactions were evaluated and the results obtained were summarized as follows : 1. The antigen consisting of a water-lysate of T gondii tachyzoites was adsorbed onto polystyrene latex particles of $0.8{{\mu}m}$ in diameter by adding a latex suspension to an equal volume of diluted antigen solution and by incubating the mixture at $37{^{\circ}C}$ under different conditions. 2. The optimum incubation buffer used for the antigen sensitization was 0.1M Tris-HCl buffer(pH 8.0). 3. The optimum serum dilution buffer used for the latex agglutination test was 0.1M Tris-HCl-NaCl buffer(pH 7.4) containing 300 mM NaCl. But 0.1M Tris-HCl-NaCl buffer(pH 7.4) containing 300-600 mM NaCl, 0.5% BSA and 0.01% Tween-20 improved the agglutination pattems and cleared the background of microplate well without the effects on L.A titer. 4. The time required for antigen sensitization was 40 and 60 min in incubation buffer(pH 8.0) at $37{^{\circ}C}$. But the optimun time for antigen sensitization was min at $37{^{\circ}C}$. 5. The optimun quantity of antigen absorbed on latex particles for proper agglutination was the range of 20 to $32{\mu}g$ of latex particles. 6. The optimun concentration of the latex-antigen suspension for the proper agglutination reaction was determined as 0.2%(w/v). 7. The specificity, rapidity and simplicity of the latex-particle agglutination test suggested that it might be adaptable to large scale serum screening.

• Archives of Plastic Surgery
• /
• 제39권1호
• /
• pp.25-30
• /
• 2012

Background : The soft palate functions as a valve and helps generate the oral pressure required for normal speech resonance. Speech problems and nasal regurgitation can result from a soft palatal defect. Reduction of the size of the velopharyngeal orifice is required to compensate for the lack of mobility in a reconstructed soft palate. We suggest a large volume folded free flap for reduction of the caliber and a palmaris longus tendon sling for suspension of the reconstructed palate. Methods : Six patients had total soft palate resection for tonsillar cancer and reconstruction with a large volume folded radial forearm free flap combined with a palmaris longus sling. A single surgeon and speech therapist examined the patients with three standardized speech assessment tools: nasometer test, consonant articulation test, and speech acuity test performed for speech evaluation. Results : Mean nasalance score was 76.20% for sentences with nasal sounds and 43.60% for sentences with oral sounds. Hypernasality was seen for oral sound sentences. The mean score of the picture consonant articulation test was 84% (range, 63% to 100%). The mean score of the speech acuity test was 5.84 (range, 5 to 6). These mean ratings represent a satisfactory level of speech function. Conclusions : The large volume folded free flap with a palmaris longus tendon sling for total soft palate reconstruction resulted in satisfactory prognosis for speech despite moderate hypernasality.

• 한국재료학회지
• /
• 제4권8호
• /
• pp.927-933
• /
• 1994

비구형 입자들의 크기와 형태에 따른 침강 특성의 영향을 검토하였다. 비구형입자를 포함하는 서스펜션의 침강에서 $log \mu_{c}$대 $log \varepsilon$로부터 얻은 기울기 지표n값은 형태와 크기가 다른 입자는 같은 부피 농도에서 흡착되는유체량이 달라져 입자크기가 감소하거나 불균일한 경우 증가하는 경향을 나타내었다. 실험결고 비구형입자를 포함하는 서스펜션의 침강에서 기울기 지표 $n_{i}$값에 대하여 $n_{i}=n(a+b/d_{v})$와 같은 식을 얻었으며 이때 a, b는 입자형태에 따른 상수이다.

• Korean Chemical Engineering Research
• /
• 제44권3호
• /
• pp.289-293
• /
• 2006

탄산칼슘을 제조하는 연속식 결정화 공정에서 결정의 입도분포에 대한 온도와 교반속도 영향을 연구하였다. 연속식조업에서 용액의 산도 변화는 적었다. 교반속도가 300 RPM 이하, 용액의 온도가 낮으면 탄산칼슘 결정의 부피 평균크기는 안정하였고, 전자현미경 사진 관찰에서 calcite와 aragonite가 만들어졌음을 알 수 있었다

• 한국윤활학회:학술대회논문집
• /
• 한국윤활학회 2004년도 학술대회지
• /
• pp.323-329
• /
• 2004

The electrical and rheological properties pertaining to the electrorheological (ER) behavior of chitosan derivatives, chitosan, chitosan ammonium salt and chitosan phosphate suspensions in silicone oil were investigated. Chitosan derivative suspensions showed a typical ER response (Bingham flow behavior) upon application of an electric field. However, chitosan phosphate suspension exhibited an excellent shear yield stress compared with chitosan and chitosan ammonium salt suspensions. The difference in behavior results from the difference in the conductivity of the disperse phases due to the difference of their polarizability. The shear stress for the chitosan, chitosan ammonium salt and chitosan phosphate suspensions exhibited a linear dependence on the volume fraction of particles and 1.18 ,1.41 and 1.67 powers of the electric field. On the basis of the experimental results, the newly synthesized chitosan derivative suspensions found to be an ER fluid.

• KSTLE International Journal
• /
• 제5권2호
• /
• pp.49-51
• /
• 2004

The electrical and rheological properties pertaining to the electrorheological (ER) behavior of chitosan derivatives, chitosan, chitosan ammonium salt and chitosan phosphated suspensions in silicone oil were investigated. Chitosan derivative suspensions showed a typical ER response (Bingham flow behavior) upon application of an electric field. However, chitosan phosphate suspension exhibited an excellent shear yield stress compared with chitosan and chitosan ammonium salt suspensions. The difference in behavior results from the difference in the conductivity of the disperse phases due to the difference of their polarizability. The shear stress for the chitosan, chitosan ammonium salt and chitosan phosphate suspensions exhibited a linear dependence on the volume fraction of particles and 1.18, 1.41 and 1.67 powers of the electric field. On athe basis of the experimental results, the newly synthesized chitosan dervative suspensions found to be an ER fluid.

• 패션비즈니스
• /
• 제18권1호
• /
• pp.164-181
• /
• 2014

As the boundary between fashion and architecture is getting blurred, the interactions of the two fields are turning out abundant as well as essential. This study investigates the tectonic strategies in architectural fashion design as a novel aesthetic in the 21st century by combining literary survey and case analysis on architecture and contemporary fashion. The tectonic strategies in the works of architectural fashion designers were categorized as follows: organic geometry, technological garment construction, and independent space. Organic geometry transforms basic geometric shapes into subtle organic forms after being thrown on the body. Technological garment construction explores the garment structure and volume by applying the structural principle of suspension and fractal geometry. Independent space refers to maintaining the firm three-dimensionality of garment structure which keeps the distance from the body, assuming the similarity to architecture.

• 패션비즈니스
• /
• 제21권3호
• /
• pp.1-13
• /
• 2017

Ralph Rucci, the first American designer who presented official collections at Paris Couture since Mainbocher, is known for his innovative dressmaking techniques and sculptural forms. Accompanied by literature survey, this study examines the aesthetics of Ralph Rucci's designs by content analysis of his works, Rucci's designs since his debut collection of 1994 until he left the Chado Ralph Rucci label in 2014 and his returning collection under the new label, RR331, in 2016. Ralph Rucci developed his creative designs by the following strategies: First, he reinvents the couture tradition of Balenciaga and $Gr{\grave{e}}s$ into progressive and functional American couture in that he gives consideration to a wearer's movements and comforts. Second, Rucci constructs architectural volume based on human anatomy and architectonic strategies, with the attention to three-dimensional quality of the space built between the body and a garment. Third, he conducts engineered construction summarized by his original 'suspension' techniques, which function as both ornamentation and construction.

• 한국전산유체공학회:학술대회논문집
• /
• 한국전산유체공학회 2007년도 추계 학술대회논문집
• /
• pp.27-31
• /
• 2007

Glass particle distribution in a stirred solid/liquid systems was investigated using computational fluid dynamics(CFD). The numerical results were compared to experimental data from the available literature which investigated the local dispersed phase volume fraction by means of an endoscope technique. Eulerian multi-phase model and applications considered high loading of solid particle was used to investigate the influence of the particle concentration and mixing tank size on the solid distribution. A good agreement was obtained between the experimental data and simulation results. The results showed different solid particle distribution in an agitator by particle concentration and mixer size.

• 한국가시화정보학회:학술대회논문집
• /
• 한국가시화정보학회 2004년도 추계학술대회 논문집
• /
• pp.84-85
• /
• 2004

Experimental study was conducted to characterize shear-induced lateral migration of $1.0-{\mu}m-diameter$ Brownian particles flowing through a rectangular microchannel which can be used to deliver small amount of liquids, drugs, biological agents and particles in microfluidic devices. Measurements were obtained by using a mercury lamp with a light of 532-nm wavelength, an inverted epi-fluorescence microscope, and a cooled CCD camera to record particle images. Peclet number was used as a parameter to assess the lateral distribution of the particles at a fixed volume fraction of $0.1\%$. It was shown that as Pe increased, particles were moved toward the centerline of the channel, which is in good agreement with previous studies.