• Journal of Plant Biotechnology
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• 제46권2호
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• pp.114-118
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• 2019

Efficient protocol for plant shoot regeneration of Brassica juncea L. CZERN was established by using organic media components and growth stimulating factors of the vermicompost and coelomic fluids. Formulated organic plant tissue culture media (Vermicompost (30%) extracts supplemented with 20 mL/L coelomic fluid) have shown maximum shoot regeneration when compared with the Murashige and Skoog (MS) medium, which were supplemented with 1 mg/L 6-benzyladenine (BA) and 0.1 mg/L of Naphthaleneacetic acid (NAA). Cotyledon explants produced the highest shoot regeneration frequency from fourday-old germinated seedlings in comparison with non-germinated seedlings. The vermicompost extracts have proved to be the best organic plant growth media to induce shoots from cotyledons compared to the MS media. Statistically significant difference (P = 0.008) for the root length, shoot length (P=0.000350) and the leaves (P=0.375) of the mustard plantlets were analyzed successfully. The survival rate was 98% in the mustard cotyledons on the Vermicompost extract media and 63% on MS media respectively. The coelomic fluid also is much suitable to induce shoots from cotyledons at lower concentrations. It was also shown that the vermicompost extract, which comprised of humic acids along with coelomic fluid, affected shoot regeneration from the cotyledons. An efficient and organic shoot regeneration study was standardized and it can be applicable in the improvement of the economically important crops.

• BMB Reports
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• 제46권1호
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• pp.31-36
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• 2013

Heat shock proteins play an important role in plant stress tolerance and are mainly regulated by heat shock transcription factors (Hsfs). In this study, we generated transgenic rice over-expressing OsHsfA7 and carried out morphological observation and stress tolerance assays. Transgenic plants exhibited less, shorter lateral roots and root hair. Under salt treatment, over-expressing OsHsfA7 rice showed alleviative appearance of damage symptoms and higher survival rate, leaf electrical conductivity and malondialdehyde content of transgenic plants were lower than those of wild type plants. Meanwhile, transgenic rice seedlings restored normal growth but wild type plants could not be rescued after drought and re-watering treatment. These findings indicate that over-expression of OsHsfA7 gene can increase tolerance to salt and drought stresses in rice seedlings.

• The Plant Pathology Journal
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• 제22권4호
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• pp.364-368
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• 2006

Survival of biocontrol agents and their effective colonization of rhizhosphere are the essential components for successful disease suppression. The effects of hydrogel supplement on bacterial survival and disease control were evaluated in pot and in the field. Addition of 2% hydrogel material to potting soil resulted in significant enhancement of colonization of biocontrol agent Serratia plymuthica A21-4 both in soil and rhizosphere of pepper plants. Rhizosphere colonization of S. plymuthica A21-4 retrieved from 40 days old pepper seedlings indicated 100 times higher bacterial population in hydrogel treated soil than in ordinary pot soil. The pepper plants sown in hydrogelated potting soil showed higher seed germination rate and the better growth of pepper plant than those in ordinary commercial pot soil. Although the suppression of Phytophthora capsid density in the potting soil by treatment of biocontrol agent A21-4 was not significantly different between in hydrogelated soil and ordinary potting soil, the suppression of Phytophthora blight between two treatments was significantly different. A21-4 treatment in hydrogelated potting soil was completely disease-free while same treatment in ordinary potting soil revealed 36% disease incidence. Our field study under natural disease occurrence also showed significantly less disease incidence(12.3%) in the A21-4 treatment in the hydrogelated soil compared to other treatments. Yield promotion of pepper by the A21-4 treatment in the hydrogelated potting soil was also recognized. Our results indicated that hydrogel amendment with biocontrol agent in pot soil would be a good alternative to protect pepper seedlings and increase plant yield.

• Journal of Plant Biotechnology
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• 제48권1호
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• pp.54-61
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• 2021

This work describe an efficient method for the shoot induction and plant regeneration of seedling-derived apical bud explants of Tilia mandshurica Rupr. & Maxim. The highest rate of shoot induction (82.2%) was obtained when apical bud explants from juvenile seedlings (5 months old) were cultured on Murashige and Skoog (MS) medium containing 1.0 mg/L 6-benzylaminopurine (BAP). However, apical bud explants obtained from mature trees (12 years old) did not produce any shoots, even with BAP supplementation. Among the three cytokinins tested for shoot multiplication (BAP, zeatin, and kinetin), BAP was the most effective; the highest number of shoots per explant (2.1) was observed on MS medium supplemented with 1.0 mg/L BAP. In contrast, the longest average shoot length (3.0 cm) was observed after growth on MS medium with 2.0 mg/L zeatin. No multiplication occurred when apical bud explants were cultured with kinetin-supplemented media. During rooting of in vitro-elongated shoots, the highest rooting rate (100%) was observed in half-strength MS medium supplemented with 0.5 ~ 1.0 mg/L indole-3-butyric acid (IBA) or 3.0 mg/L 1-naphthaleneacetic acid (NAA). During the acclimatization process, plantlets that were rooted on the IBA (0.5 mg/L)-supplemented medium had the highest survival rate (100%) and maximum root length (18.5 cm). These findings suggest that a low concentration (0.5 mg/L) of IBA is appropriate for the rooting and acclimatization of T. mandshurica. Plants were successfully transferred to the greenhouse with a 100% survival rate. This protocol will be useful for the large-scale propagation of Tilia species.

• Journal of Microbiology and Biotechnology
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• 제15권5호
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• pp.1011-1021
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• 2005

Rhizobacteria are actively sought for the substitution of chemical fertilizers and pathogen control agents in environment-friendly sustainable agriculture. To be successfully commercialized in the current Korean market as agriculture biomaterials, microbial agents should exhibit both properties of plant growth promotion and pathogen control. That is, the organism must be a phytostimulator as well as a biocontrol agent. These criteria and the survival rate of a rhizobacterium, Bacillus amyloliquefaciens 7079, in the soil system were investigated to evaluate the suitability for future commercialization. B. amyloliquefaciens 7079-treated seedlings showed $22.8\%$ maximum increase in leaf-length growth, compared with water-treated controls, showing the phytostimulating property. The disease suppression rates of Phytophthora-blight of peppers and Fusarium-wilt of tomatoes by B. amyloliquefaciens 7079 were 1.5 and 2.2 times better, respectively, than by three popular chemical fungicides used in actual agricultural practices to control the respective pathogens. Survival of B. amyloliquefaciens 7079 on the rhizoplane and in the rhizosphere was favorable up to 50 days in the soil system employed. These positive properties show that B. amyloliquefaciens 7079 is likely to be a suitable candidate for commercialization to market as agricultural biomaterials.

• 한국산림과학회지
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• 제109권4호
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• pp.390-399
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• 2020

본 연구는 조림목 묘령과 식재밀도가 느티나무 조림목의 초기 생육 특성에 미치는 영향을 알아보고자 수행되었다. 2017년 3월말에 온대 남부지역인 영덕지역 2 ha 면적에 묘령[용기묘(1-0), 용기묘(2-0)] × 식재밀도(3,000본 ha-1, 5,000본 ha-1) 4가지 조합으로 느티나무 묘목을 식재하였다. 각 처리별 3개의 조사구를 설치하였고, 조림목의 근원경과 수고 생장을 매년 9월마다 3년 동안(2017-2019년) 측정하여 수간 재적을 계산하였다. 느티나무의 초기생존율은 처리간에 차이를 나타내지 않았다. 수고는 묘령(1-0)과 묘령(2-0)에서 밀식처리의 효과가 나타나지 않았으나, 근원경과 H/D율은 묘령(2-0)에서 식재 후 3년차에 밀식처리 효과가 나타났다. 개체목당 수간재적은 묘령(2-0) 5,000본 ha-1 처리구에서 가장 높았으며, 묘령(1-0) 3,000본 ha-1 처리구에서 가장 낮았다. 수간재적은 묘령과 식재밀도 간에 상호작용 효과는 없었고, 묘령과 식재밀도가 높을수록 높은 수간재적을 보였다. 단위면적당 수간 재적은 용기묘(1-0) 3,000본 ha-1 대비 용기묘(1-0) 5,000본 ha-1는 85%, 용기묘(2-0) 3,000본 ha-1와 5,000본 ha-1는 각각 약 68%, 300% 향상되었다. 본 연구는 식재밀도가 묘령에 따라 생장에 다르게 영향을 미친다는 것을 보여주며, 용기묘(2-0)의 초기 생장을 향상시키는데 5,000본 ha-1 처리가 적합하다는 것을 제안한다.

• 한국산림과학회지
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• 제99권3호
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• pp.359-367
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• 2010

본 연구에서는 소나무 용기묘(1-0묘)를 대상으로 계절별 식재시기 및 조림지 하층식생 조건에 따른 생존율과 생장 특성을 조사하여 용기묘 조림과정에서 보다 유리한 적정 식재시기를 구명하고자 하였다. 또4한 용기묘의 식재기간 확대 가능성을 검토하는데 목적이 있다. 식재시기는 봄, 여름, 가을의 세 처리를 하였으며, 시험지는 하층식생이 무성한 지역(A)과 그렇지 않은 지역(B)으로 나누어 처리하였다. 소나무 용기묘의 생존율은 모든 시험지에서 여름 식재가 가장 우수한 것으로 나타났다. 소나무 용기묘의 근원경과 묘고 생장은 여름 식재가 가장 우수한 값을 보였으며, 식재 후 초기에는 생장이 불량했지만 시간이 지날수록 생장률이 점점 높아졌다. 대부분의 식재시기 처리에서 B 지역이 A 지역보다 생장률이 우수한 것으로 나타났다. 물질생산량에 있어서도 세 시험지 모두 여름 식재가 가장 높았으며, 하층식생이 발달하지 않은 B 지역이 가장 높았다. 이 연구를 통하여 소나무 용기묘는 여름 조림이 가능하여 식재기간을 기존의 봄과 가을에서 여름까지 확대시킬 수 있으며, 높은 생존율과 생장을 위한 하층식생 관리의 중요성을 보여주고 있다.

• 한국조경학회지
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• 제29권4호
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• pp.82-90
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• 2001

This study was carried out to reveal optimum conditions for in vitro propagation of 3 Korean native azalea species, Rhododendron mucronulatum, R. yedonese var. poukhanense, and R. shlippenbachii, which are useful for landscape proposes. Seeds and meristems from three azalea species were cultured on 1/2MS, Hyponex, and Anderson media containing growth of regulators benzyladenine(BA) and 2-isopentenyadenosine(2ip). The results were as follows. 1. In the culture of R. schlippenbachii and R. mucronulatum seeds, in vitro seedlings germinated and grew well on he 1/2MS and Anderson media, while R. yedoense var. poukhanense on Hyponex media containing 6.0mg/$\ell$ 2ip. 2. When the meristems of R. mucronulatum were cultured on Andeson media containing 9.0mg/$\ell$ 2ip, the survival rate of meristems was 23.0% in 6 weeks after culture, and the survival rate of R. schlippenbachii was 46.0% o nthe same media containing 12.0mg/$\ell$ 2ip. The survial rate of R. yedoense var. poukhanense was 92.0% onHyponex media containing 0.5mg/$\ell$ BA and 9.0mg/$\ell$ 2ip. When the meristems of R. mucronulatum and R. yedoense var. poukhanense were cultured on Hyponex media containing 12.0mg/$\ell$ 2ip, they showed the most excellent growth. R. schlippenbachii grew well on Anderson media containing 9.0mg/$\ell$ 2ip. When in vitro shoots of R. yedoense var. poukhanense were subcultured to solid medium, they grew well in shoot growth on Hyponex media containing 6.0mg/$\ell$ 2ip.

• The Korean Journal of Ecology
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• 제23권6호
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• pp.445-452
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• 2000

The effects of sludge and fertilizer application on germination and seedling growth of woody plants on heavy metal mine tailings were evaluated by greenhouse experiment. Two different mine tailings (Lead-zinc mine tailings from Kwangmyong, Kyonggi-do and tungsten mine tailings from Sangdong. Kangwon-do). four fertilizer treatments (N +P +K: 20, 40, 60, and 80 kg/m$^3$), and four sewage sludge treatments (5.5, 11, 22.5, and 45 Mg/m$^3$) were used in the experiment. Tested plants were Pinus densiflora, Larix leptolepis, Amorpha fruticosa, and Alnus hirsuta. There were three replicates for each treatment. In addition, vermiculite was used instead of mine tailings to determine the effect of physical amendments. Fifty seeds of a species were sown in a pot (upper diameter 13.5 cm, depth 10 cm) and seedling emergence were recorded daily for 30 days. The highest germination rate was 53% for all treatments. Germination rate of Larix leptolepis was lowest among the four species studied. One month later after seeding, seedlings were thinned and only 5 seedling were left in each pot, and fertilizer and sewage sludge were applied once again. Growth of seedlings were determined for 10 weeks since then. Most plants grew very poorly or died within 5 weeks on lead/zinc mine tailings from Kwangmyong. The analysis of heavy metal contents by the total dissolution method showed that heavy metals generally increased in the order of tungsten mine tailings from Sangdong < sewage sludge from Puchon < lead/zinc mine tailings from Kwangmyong. Growth of woody plants was improved significantly by the fertilizer treatments on tungsten mine tailings. In contrast. survival and growth of woody plants were not affected significantly by the sewage sludge treatment on both tailings. This study shows that fertilizer applied to established seeded stands may provide some benefits in terms of increased ground cover in the field. It is suggested that reclamation should be proceeded by the study of the physico-chemical and biological characteristics of mine tailings.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 1993년도 Proceedings of International Conference for Agricultural Machinery and Process Engineering
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• pp.517-525
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• 1993

In farms, forestry centres and greenhouses, the system can be used in growing seedlings of crops , breeding of plant and vegetables in cold areas. It may control all the activities automatically in temperature, humidity , sprinkling and heating. This is a closed-cycle control system controlled automatically by single-chip microcomputer. (MCS-51) The purpose of this system is to shorten the experimental cycle of crops , improve the survival rate of crops breeding, and ensure the growth of vegetables in the cold areas. Therefore, it can be used widely.