• Korean Journal of Environment and Ecology
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• v.21 no.6
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• pp.544-553
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• 2007

This study is focused on the cultivation of seedlings and cuttings of roundleaf chastetree(Vitex rotundifolia L. f.) according to the sorts of soil and fertilization methods. The results are summarized as follows: When roundleaf chastetree seedlings(aged 1-0) and cuttings(aged 1-0) were cultivated in three different kinds of soil- sand, granite soil, and yellow silt- without any fertilization, 70% of the seedlings survived in sand and granite soil, and 35% did in yellow silt; 90% of the cuttings survived in granite soil and 80% did in sand and yellow silt. The general growth of both seedlings and cuttings was good in granite soil, and sand but relatively inferior in yellow silt. Seedlings showed good stem growth while cuttings had fine root growth. In case of cultivation of seedlings with different kinds and quantities of fertilizer adjusted and with granite soil as basic soil, seedlings and cuttings survived in the experimental section where only the organic fertilizer was used as basic fertilization; in contrast, seedlings grown in the experimental section were all dead where both inorganic and liquefied fertilizer were used. Cuttings showed considerably higher survival rates than seedlings at the experimental fertilization section; particularly, the survival rate of cuttings was twice as much as that of seedlings at the experimental section with low fertilization. This study results revealed that cuttings are desirable than seedlings in general rootage, growth, adaptability to soil, and cuttings have lower demand for soil fertility in time of growing roundleaf chastetree saplings.

• Journal of Aquaculture
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• v.8 no.4
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• pp.251-260
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• 1995

Experimental transportations of the olive flounder (Paralichthys olivaceus) seedlings (body weight $2.1\pm0.39g$) with polyethylene vinyl bag were Performed to study the change of dissolved oxygen (DO) during transportation, survival rate and feeding activity of seedlings after transportation. The distance and time required for the transportations were 272 km and 11 hours, respectively. DO was rapidly decreased within 3 hours (P<0.05) during the transportation of seedlings of 40 fish in 10l of sea water at $15.6\~17.8^{\circ}C$ of initial water temperature with 5 ml/l of initial DO. The decreasing tendency of DO (Y) according to elapsed time (X) was expressed an equation of $Y\;=\;2.7444X^{-0.4780}$ (r=0.8071). Feeding activity of the seedlings after transportation supplemented with oxygen and icepack besides above transport conditions was significantly higher than that of fish supplemented with icepack only (P<0.05). It was desirable to starve the seedlings for 36 hours before transportation. Survival rate of seedlings was greatly reduced, when the dissolved oxygen concentration was lowered below the range of $0.7\~0.94ml/l$ just after transportation.

• Horticultural Science & Technology
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• v.31 no.4
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• pp.400-406
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• 2013

This study was conducted to investigate the growth and flowering of African marigold (Tagetes erecta L.) and salvia (Salvia splendens F. Sello ex Ruem & Schult.) seedlings before and after storage under fluorescent lamps and green LED radiation conditions with different light intensities during storage. The both seedlings were kept under a storage room controlled at $8^{\circ}C$ air temperature and $40{\pm}10%$ relative humidity conditions. Light intensities were maintained at 15 and $30{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ during storage. In lighting treatments, dry weight of African marigold at 28 days after storage was not significantly different, and decreased approximately 29% compared to pre-storage under dark treatment. There was no significant difference in the leaf area of salvia seedlings stored under dark condition compared to before storage, but the leaf area under green light radiation with higher light intensity (treatment GH) was two times greater than before storage. The survival rate after transplanting of African marigold stored under dark condition was 10%, and days to flowering increased compared to those stored under fluorescent and green light with higher light intensity (treatment FLH, GH). Comparing to before storage, growth and flowering of the both seedlings after storage were significantly promoted by the light exposure during storage. The present experimental results show that the light intensity should be decided to maintain minimum growth during lighting storage and storage quality of the seedlings such as flowering promotion and extended blooming period after lighting treatment during storage period from the above results.

• ALGAE
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• v.37 no.2
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• pp.105-121
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• 2022

Modern seaweed farming relies heavily on seedlings from natural beds or vegetative cuttings from previous harvests. However, this farming method has some disadvantages, such as physiological variation in the seed stock and decreased genetic variability, which reduces the growth rate, carrageenan yield, and gel strength of the seaweeds. A new method of seedling production that is sustainable, scalable, and produces a large number of high-quality plantlets is needed to support the seaweed farming industry. Recent use of tissue culture and micropropagation techniques in eucheumatoid seaweed production has yielded promising results in increasing seed supply and growing uniform seedlings in large numbers in a shorter time. Several seaweed species have been successfully cultured and regenerated into new plantlets in laboratories using direct regeneration, callus culture, and protoplast culture. The use of biostimulants and plant growth regulators in culture media increases the seedling quality even further. Seedlings produced by micropropagation grew faster and had better biochemical properties than conventionally cultivated seedlings. Before being transferred to a land-based grow-out system or ocean nets for farming, tissue-cultured seedlings were recommended to undergo an acclimatization process to increase their survival rate. Regular monitoring is needed to prevent disease and pest infestations and grazing by herbivorous fish and turtles during the farming process. The current review discusses recent techniques for producing eucheumatoid and other valuable seaweed farming materials, emphasizing the efficiency of micropropagation and the transition from laboratory culture to cultivation in land-based or open-sea grow-out systems to elucidate optimal conditions for sustainable seaweed production.

• Korean Journal of Medicinal Crop Science
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• v.11 no.1
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• pp.46-52
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• 2003

This experiment was conducted to investigate the propagation for mass production of Acanthopanax koreanum NAKAI utilized for health food and medicine material. Germination rate of A. koreanum seeds were 64% when seeds were stored at 15 for 60 days and then were treated with 50 ppm of kinetin and dormancy were broken at 5 for 60 days. Rooting rates of green-wood cuttings treated with IBA 100 ppm, NAA 50 ppm, and IAA 100 ppm were 61.7, 56.7, and 60.0%, respectively. Rooting rates of greenwood cuttings treated with Rooton in scoria + horticulture media and volcanic ash were 76.7 and 66.7%, respectively. Survival rate of seedlings planted on Aug. 10 was highest (96%) under 75% shading net while shoot growth was best under 55% shading net. Survival rate of seedlings planted on May 2 was highest (91%) 55% shading net, and shoot growth was also best 55% under shading net.

• The Korean Journal of Mycology
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• v.45 no.3
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• pp.188-195
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• 2017

Despite the high commercial value of the pine mushroom (Tricholoma matsutake) around the world, its production still depends upon natural harvesting. In recent years, mushroom researchers in Korea and Japan have been successful in artificially cultivating T. matsutake by producing single mushroom through matsutake-infected pine seedlings. In this study, we determined the minimum seedling height required for the survival of matsutake mycelia in the infected pine seedlings in the field. The survival rate of matsutake mycelia in the matsutake-infected pine seedlings was 50% (14) in two years and 71% (10) in one year after their transplantation. The average height at time of transplantation of the matsutake-infected pine seedlings that bore surviving mycelia after transplantation was 25 cm (minimum 12 cm to maximum 40 cm). In conclusion, the matsutake mycelium of matsutake-infected pine seedlings was able to survive in field conditions if the height of the seedling at the time of T. matsutake infection was at least 12 cm. These results suggest that the height of the host plants used in conventional matsutake-infected pine seedling production should be greatly reduced to improve the matsutake cultivation. Therefore, standardization of the seedling height for artificial cultivation of pine mushrooms by the matsutake-infected pine seedling method is suggested.

• Journal of Bio-Environment Control
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• v.29 no.3
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• pp.219-230
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• 2020

This study was conducted to determine the optimum medium and humidification period for the strawberry (Fragaria × ananassa Duch. cv. Maehyang) cutting seedling production. The cuttings were planted in coir (CO), rockwool (RW), phenolic foam (PF), and organic foam medium (OFM) with fogging treatment in 0, 3, 6, 9, or 12 days on February, 2019. And, as the field verification examination research, cuttings were planted in CO, RW, PF, and OFM, with misting treatment in 0, 6, 9, 12, or 15 days on June, 2019. In fogging treatments, rooting and survival rates of seedlings tended to increase with longer fogging periods, and rooting and survival rates were showed significantly higher in CO medium with more than 9 days of fogging periods. In misting treatments, rooting rate was significantly higher in CO and RW medium with more than 9 days of misting periods, and survival rate was significantly higher with more than 12 days of misting periods. There was no significantly difference by growth medium and humidification period in shoot and root growth. Therefore, when considering the rooting and survival rate the CO medium with 9 days of humidification period could be beneficial for the production of the 'Maehyang' strawberry cutting seedlings.

• Korean Journal of Environmental Biology
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• v.35 no.2
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• pp.152-159
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• 2017

For the effective seedling production of the hard shelled mussel, Mytilus coruscus, this study assessed the effects of the dietary value of live food, density, water temperature and salinity on growth and survival rate of the larvae. The optimal survival rate and growth rate were examined under differing conditions of water temperature, salinity, and rearing density for 30 days. The three groups were provided different feeding organisms, such as Isochrysis galbana and Teleaulax suecica. The mixtures were provided at a rate of $5{\times}10^4cell\;mL^{-1}$. The best growth was observed in the group with conditions $21^{\circ}C$ water temperature ($16.2{\pm}9.1{\mu}m$), 33 psu of salinity ($16.82{\pm}3.9{\mu}m$), $2500individual\;m^{-2}$ ($17.2{\pm}5.9{\mu}m$), and fed with $5{\times}10^4cell\;mL^{-1}$ of I. galbana and T. suecica mixture ($16.0{\pm}7.3{\mu}m$). The highest survival rate was found in the group at conditions $18^{\circ}C$ water temperature (66.4%), 33 psu of salinity (24.4%), $2500individual\;m^{-2}$ (65.8%), and fed with $5{\times}10^4cell\;mL^{-1}$ of I. galbana and T. suecica mixture (58.8%). We therefore conclude that the suitable culture conditions for the stable production of hard shelled mussel artificial seedlings was at 18 to $21^{\circ}C$ of temperature, 30 to 33 psu of salinity, 2500 to $5000individual\;m^{-2}$ of rearing density, and feeding supplement of $5{\times}10^4cell\;mL^{-1}$ of I. galbana and T. suecica mixture under semi running water system.

• Journal of Climate Change Research
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• v.7 no.1
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• pp.77-84
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• 2016

Seedling stage is particularly important for tree survival and is easily influenced by warming. Therefore, air temperature being increased due to climate change may affect physiological traits and growth of seedlings. This study was conducted to investigate the physiological and growth responses of Larix kaempferi seedlings to open-field experimental warming. 1-year-old and 2-year-old L. kaempferi seedlings were warmed with infrared lamps since April 2015 and April 2014, respectively. The seedlings in the warmed plots were warmed to maintain the air temperature to be $3^{\circ}C$ higher than that of the control plots. Physiological responses (stomatal conductance, transpiration rate, net photosynthetic rate and total chlorophyll content) and growth responses (root collar diameter (RCD), height and biomass) to experimental warming were measured. Physiological and growth responses varied with the seedling ages. For 2-year-old L. kaempferi seedlings, stomatal conductance, transpiration rate and net photosynthetic rate decreased following the warming treatment, whereas there were no changes for 1-year-old L. kaempferi seedlings. Meanwhile, total chlorophyll content was higher in warmed plots regardless of the seedling ages. Net photosynthetic rate linked with stomatal conductance also decreased due to the drought stress and decrease of photosynthetic efficiency. In response to warming, RCD, height and biomass did not show significant differences between the treatments. It seems that the growth responses were not affected as much as physiological responses were, since the physiological responses were not consistent, nor the warming treatment period was enough to have significant results. In addition, multifactorial experiments considering the impact of decreased soil moisture resulting from elevated temperatures is needed to explicate the impacts of a wide range of possible climate change scenarios.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.23 no.4
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• pp.59-67
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• 2020

In this study, the effects of windbreak fences composed of natural vegetation on one-year-old seedlings were analyzed to develop restoration methods for an endangered subalpine species, the dwarf Siberian pine (Pinus pumila (Pall.) Regel). One-year-old seedlings were planted in 2016 by sowing seeds that had been collected from the Daecheongbong area on Mt. Seoraksan, South Korea, in 2014. The area near Daecheongbong was selected as the experimental site, and treatment and control plots (2m×2m) were installed at the site. To analyze the effects of wind protection, windbreak fences were constructed in the treatment plots using hairy Korean rhododendrons (Rhododendron mucronulatum Turcz. var. ciliatum Nakai) from the surrounding area and weather stations were installed to investigate atmospheric temperature, humidity, and wind speed. In all control plots without windbreak fences, dwarf Siberian pine seedlings were killed by strong winds seven months after planting. In contrast, the average survival rate of the seedlings in treatment plots was 96.7% after seven months, 64.2% after two years, and 45% after three years, with most (85.3%) of the seedlings showing good initial root establishment. Accordingly, windbreak fences composed of natural vegetation are suitable for promoting the early establishment of seedlings in the restoration of dwarf Siberian pine stands.