• Title/Summary/Keyword: surface aeration

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High-Cell-Density Fed-Batch Culture of Saccharomyces cerevisiae KV-25 Using Molasses and Corn Steep Liquor

  • Vu, Van Hanh;Kim, Keun
    • Journal of Microbiology and Biotechnology
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    • v.19 no.12
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    • pp.1603-1611
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    • 2009
  • High-cell-density cultivation of yeast was investigated using the agricultural waste products corn steep liquor (CSL) and molasses. The Saccharomyces cerevisiae KV-25 cell mass was significantly dependent on the ratio between C and N sources. The concentrations of molasses and CSL in the culture medium were statistically optimized at 10.25% (v/v) and 16.87% (v/v), respectively, by response surface methodology (RSM). Batch culture in a 5-l stirred tank reactor using the optimized medium resulted in a cell mass production of 36.5 g/l. In the fed-batch culture, the feed phase was preceded by a batch phase using the optimized medium, and a very high dried-cell-mass yield of 187.63 g/l was successfully attained by feeding a mixture of 20% (v/v) molasses and 80% (v/v) CSL at a rate of 22 ml/h. In this system, the production of cell mass depended mainly on the agitation speed, the composition of the feed medium, and the glucose level in the medium, but only slightly on the aeration rate.

Membrane Concentrate Thickening by Hollow-fiber Microfilter in Drinkin Water Treatment Processes

  • 이병호
    • Membrane Journal
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    • v.1 no.1
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    • pp.100-100
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    • 1991
  • A novel system to thicken the concentrated colloidal solution from membrane water treat-ment processes was developed. A hollow-fiber microfilter(hydrophilic polyethylene nominal pore size 0.1 μm total surface area 0.42 m2) was installed in an acrylic housing that has an aeration port 5 cm below the membrane and a clarifier in the bottom. The concentrate was uniformly supplied from the top of the housing. Bacuum filtration caused downward flow of concentrate and as a result thickening interface. The addition of poly-aluminum chloride (PAC) resulted in rapid increase of trans-membrane pressure (TMP) and in no improvement of the filtered water turbidity and thickening process. Two types of con-centrate and concentrate turbidity had little effect on the increase of TMP and concentrate thickening. It was observed that for the same height of membrane housing membrane surface area to housing volume (A/V) ratio had significant effect on the increase of TMP. When the housing volume was increased ten times the increasing rate of TMP was three times faster as compared to the original housing. A hydraulic model successfully simulated the formation and sedimentation of thickening interface.

Development of Depot Encircled with Oxidation Canal for Water Quality Improvement (수질 개선을 위한 침전산화수로 개발)

  • 김원장;박상현
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.42 no.6
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    • pp.83-89
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    • 2000
  • A depot encircled with contact oxidation canal has been developed to improve water qualityr. The depot is aimed to settle suspended solid in the flow. The oxidation canal is to contact the pollutants and oxygen in the surface of gravel filter to purify the water. The deposit of the pollutant in the depot is released by the drainage culvert located at the bottom of it. Aeration nozzle is installed to supply enough oxygen to the gravel filter layers in the bottom of the detent the flow and improve oxidation efficiency. From the experiment it is founded that 91% of SS, 39% of TN, 63% of TP and 77% of COD were removed in the system. The treatment efficiency of total nitrogen is rather lower than any other components, however, it would be higher when some floating vegetation is added in the primary clarifier. The depot and oxidation canal system would be useful to purify the flows in the mouth of the reservoirs or at the outlet of the drainage canal.

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임기광산 폐석적치장의 수리침투특성 분석

  • 지상우;정영욱;임길재
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2004.09a
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    • pp.394-398
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    • 2004
  • This study was carried out to plan the prevention of the generation and discharge of acid mine drainage (AMD). Hydraulic characteristics were tested with the disk tension infiltrometer around the waste rock dump of the Imgi abandoned pyrophyllite mine in Busan, Korea. Because the waste rock dump of the Imgi mine have very low infiltration rate, most of rain was expected flowing into adjoined stream through the slope or plane as surface flow rather then throughflow or ground water. But slopes of the waste rock dump have many 'V' type erosion gullies and consist multi-layers. These gullies and multi-layers have coarse clastic particle layer which have very large hydraulic conductivity. So through these coarse clastic particle layers a large part of rain flow into ground. And also these layers could be played a function of aeration path, which induced oxidation of sulfide minerals and generation of AMD continuously.tinuously.

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Treatment of Saline Wastewater by the Activated Sludge with Nonwoven Fabric Separation

  • Moon, Byung-Hyun;Heo, Jong-Soo;Choi, Hyoung-Sub
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.2 no.2
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    • pp.136-140
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    • 1997
  • Direct membrane separation using nonwoven fabric was applied to saline wastewater treatment by an activated sludge process. A nonwoven fabric module was immersed in an aeration tank. The part of treated was filtered through the module by suction and the rest of that was separated by a settling tank. Various F/M ratios and salt concentrations were applied to investigate stable flux as well as pollutant removal. The pollutant removal efficiencies of nonwoven fabric separation was not affected by F/M ratios and salt concentrations and was higher than that of settling tank separation. The decline in flux was seemed to be caused by the biofilm on nonwoven fabric surface.

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Optimization for the DXAMase production from Lipomyces starkeyi using statistically designed experiments

  • Park, Jun-Seong;Gang, Hui-Gyeong;Gang, Seong-Ju;Kim, Byeong-Hun;Park, Don-Hui;Kim, Do-Man
    • 한국생물공학회:학술대회논문집
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    • 2002.04a
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    • pp.211-214
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    • 2002
  • The optimal condition for the production of DXAMase, containing the both characteristics of dextranase and amylase, was studied based on different levels of pH, temperature, and aeration rate. Response surface methodology was applied to find the optimatic condition showing the relationship between the fermentation response(dextranase and amylase activity of DXAMase) and the fermentation variables(pH, temperature, and agitation rate). In case of dextranase activity, the condition of pH 4.06, $28.08^{\circ}C$, and 235.14 rpm showed the highest activity, 2.26 U/ml, and for amylase activity, the condition of pH 4.01, $27.96^{\circ}C$, and 212.01 rpm showed the highest activity, 3.52 U/ml. For the production of DXAMase, dextranase and amylase, the optimum condition was pH 4.06, $28.08^{\circ}C$, and 234.80 rpm.

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A Study on the Locus of Dynamically-Loaded Journal Bearing with Aerated Lubricant (윤활유의 공기 혼입에 따른 동하중 조건에서의 저널 궤적에 관한 연구)

  • 박영환;장시열
    • Proceedings of the Korean Society of Tribologists and Lubrication Engineers Conference
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    • 2001.11a
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    • pp.220-228
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    • 2001
  • Journal locus with aerated lubricant is analyzed under the dynamic loading condition. In this analysis, we have found that aerated lubricant influences two major factors on the film formation. One is the density variation of the lubricant due to the volume change by the bubbles and the other is the viscosity changes of the lubricant due to the surface tension of the bubble. Those two major factors surprisingly increase the load capacity in certain ranges of bubble sizes and densities. Modified Reynolds'equation is developed with the consideration of aerated ratio in the lubricant and journal locus is computed with Mobility method with the computation of two dimensional pressure distribution over the bearing area.

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Study on The Oxygen Transfer Properties of Airlift Pump (에어리프트 펌프의 산소 전달 특성에 관한 연구)

  • OH Se-Kyung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.30 no.3
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    • pp.451-458
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    • 1997
  • The oxygen transfer coefficient was not significantly affected by either flow pattern, initial bubble size or the wastes present in the water studied. Surface active substances in the water did however influence the transition from bubble to slug flow. Airlift with length to diameter ratios less than 50 suffered from considerable losses in efficiency. When properly designed, airlift pumping efficiencies were comparable to those of conventional centrifugal pumps and the oxygen transfer efficiency were as high as or higher than those of diffused aeration systems.

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Experimental Study of Overtopping Void Ratio by Wave Breaking (쇄파에 의한 월파의 기포분율에 대한 실험적 연구)

  • Ryu, Yong-Uk;Lee, Jong-In
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.20 no.2
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    • pp.157-167
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    • 2008
  • The aeration of an overtopping wave on a vertical structure generated by a plunging wave was investigated through laboratory measurements of void fraction. The overtopping wave occurring after wave breaking becomes multi-phased and turbulent with significant aeration, so that the void fraction of the flow is of importance. In this study, fiber optic reflectometer and bubble image velocimetry were employed to measure the void fraction, velocity, and layer thickness of the overtopping flow. Mean properties were obtained by ensembleand time-averaging the repeated instantaneous void fractions and velocities. The mean void fractions show that the overtopping wave is very high-aerated near the overtopping wave front and relatively low-aerated near the deck surface and rear free surface of the wave. The flow rate and momentum of the overtopping flow estimated using the measured data show that the void ratio is an important parameter to consider in the multiphase flow. From the similarity profiles of the depth-averaged void fraction, velocity, and layer thickness, one-dimensional empirical equations were obtained and used to estimate the flow rate and momentum of the overtopping flow.

Changes in the Soil Physical Properties of Vineyard Converted from Paddy Field (논에서 전환한 포도원의 토양물리적 특성변화)

  • Yun, Eul-Soo;Jung, Ki-Youl;Park, Ki-Do;Ko, Jee-Yeon;Lee, Jae-Saeng;Park, Sung-Tae
    • Korean Journal of Soil Science and Fertilizer
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    • v.42 no.3
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    • pp.145-151
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    • 2009
  • This study was conducted to develop rational soil management and enhance the productivity of lands converted from paddy soils. Specifically, the changes in the soil physical properties brought about by the change in land usage from paddy soil were evaluated. This was carried out from 1999 to 2001 at 50 site in large-scale converted paddy fields of Kimcheon, Youngcheon, Gyeongsan and Milyang in the Youngnam region, categorized according to soil texture and drainage class. The ridge height of converted paddy soils was higher in coarse-textured and poorly-drained soils than in fine-textured and well-drained soils. The gray color of the surface soil was of lesser degree in converted soils than paddy soils and more notable in welldrained soils. The porosity ratio and the formation of aggregate structure were higher, and the appearance of soil mottling was deeper in converted paddy fields than in paddy soils. The glaying layer "g" of surface soil degraded with time. The porosity and amount of water stable aggregate was found to increase with time after conversion. The penetration resistance of the converted paddy soil was lower and deeper with time after conversion. The soil aeration of the converted paddy soil was lower in sandy loam than in loamy soil. Furthermore, soil aeration was influenced by ridge height and drainage class in poorly-drained soils.