• BMB Reports
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• v.40 no.2
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• pp.290-294
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• 2007

Saxatilin is a disintegrin known to inhibit tumor progression in vivo and in vitro. The role of saxatilin in cancer cell invasion was examined by a modified Boyden chamber assay in MDAH 2774 human ovarian cancer cell line. Saxatilin (50 nM) significantly inhibited cancer cell invasion induced by tumor necrosis factor-$\alpha$ (TNF-a$\alpha$). Saxatilin also reduced MMP-9 mRNA levels in cancer cells in a dosedependent manner. In addition, TNF-$\alpha$-induced MMP-9 activity was reduced by the treatment of saxatilin. These results indicate that transcriptional regulation of MMP-9 is an important mechanism for the tumor suppressive effects of saxatilin in MDAH 2774 human ovarian cancer cells.

• Korean Journal Plant Pathology
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• v.11 no.4
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• pp.287-291
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• 1995

Biocontrol agents, Gliocladium virens G872B and Pseudomonas putida Pf3, were compatible each other in colonizing cucumber rhizosphere, which contributed to a long-term inhibition of cucumber Fusarium wilt. G872B colonized successfully on the cucumber root system, irrespective of the introduction of Pf3. Pf3 also colonized well in the cucumber rhizosphere regardless of the presence of G872B. The individual strains effectively suppressed cucumber wilt up to 56 days after transplanting. The combined treatment of G872fB and Pf3 provided a long-term protection of about 80 days with the efficacy greater than that obtained by any individual strains under greenhouse conditions. These results suggest that the colonization of the biological control agents in the rhizosphere could be correlated directly to Fusarium wilt-suppressive potentials.

• Proceedings of the Korea Environmental Mutagen Society Conference
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• 2003.05a
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• pp.103-104
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• 2003

• Microbiology and Biotechnology Letters
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• v.23 no.1
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• pp.91-97
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• 1995

The 543 microbial strains were isolated from the rhizosphere in ginseng root rot-suppressive soil. From these, isolate KGM-100 was finally selected for the screening of powerful antagonistic bacterium for the soil-born Fusarium solani causing root rot of many important crops. The isolate KGM-100 was identified as Pseudomonas aeruginosa. Antibiotics produced by the P. aeruginosa KGM-100 were partially characterized as heat-stable and low-molecular weight antibiotics. The strain also produced siderophore, which was assumed to be pseudobactin. Pot test showed approximately 30-50% biological control effects when Fusarium solani-infected soil was treated with the P. aeruginosa KGM-100.

• Journal of Microbiology and Biotechnology
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• v.16 no.7
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• pp.1010-1016
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• 2006

The effects of the cell viability and integrity of Bifidobacterium on suppression of allergy were investigated. C3H/HeJ mice were sensitized on weeks 3, 4, 6, and 8 with ovalbumin and choleratoxin to induce an allergic reaction. Mice fed 0.2% of live, disrupted, or heat-killed Bifidobacterium bifidum BGN4 in the pellets of their diet for 8 weeks starting 2 weeks before initial sensitization differentially suppressed the allergy response in terms of levels of IgE and IgG1 in their sera, and symptoms on their tails. Viable Bifidobacterium was more effective than disrupted or heat-killed cells in suppressing the allergy. Growth inhibition, which occurred in the sham group at week 4, did not occur in the treated groups. These results show that Bifidobacterium has a suppressive effect on the allergic response of mice, and that the viability and integrity of the Bifidobacterium is required for effective suppression in our experimental model.

• Korean Journal of Pharmacognosy
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• v.16 no.1
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• pp.26-30
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• 1985

These studies were conducted to investigate the effect of Yijin-tang on the anticonvulsion, analgesic, sedative actions, effect on blood vessels and blood pressure. The result of these studies was summarized as follows; Suppressive action was shown on convulsion due to cerebrocortical causes, but no such actions were noted either myelic or diencephaltic causes in mice. Analgesic and sedative actions were significantly recognized in mice. Increasing of blood pressure and vasocontractive actions were noted.

• Molecular & Cellular Toxicology
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• v.5 no.3
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• pp.243-249
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• 2009

Melamine, which is used to produce melamine resin for various industrial applications, has a high nitrogen content by mass. For this reason, it has been illegally added to foods to increase their apparent protein content. In the present investigation, melamine-induced oxidative damage of human lymphocyte DNA was evaluated by Comet assay. The in vitro oxidative DNA damage caused by melamine increased in a dose-dependent manner. This DNA damage was significantly inhibited by treatment with ascorbate. Moreover, the traditional Korean medicinal herb, named Acanthopanax, red ginseng and green tea markedly reduced the DNA damage. Various edible plant extracts also inhibited melamine-induced oxidative DNA damage in vitro. Melamine enhanced intracellular ROS generation, and this effect was suppressed by treatment with various antioxidants.

• Proceedings of the Korean Society of Toxicology Conference
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• 2001.10a
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• pp.131-131
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• 2001

Prostaglandins and nitric oxide produced by inducible cyclooygenase (COX-2) and nitric oxide synthase (iNOS), respectively, have been implicated as important mediators in the process of inflammation and carcinogenesis. On this line, the potential COX-2 or iNOS inhibitors have been considered as anti-inflammatory and cancer chemopreventive agents.(omitted)

• Journal of Applied Biological Chemistry
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• v.50 no.3
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• pp.136-143
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• 2007

Despite the increased interests in biological control of soilborne diesease for environmental protection, biological control of bacterial wilt caused by Ralstonia solanacearum have not provided consistent or satisfying results. To enhance the control efficacy and reducing the inconsistency and variability, combinations of specific strains of microorganisms, each having a specific mechanism of control, were applied in this study. More than 30 microorganisms able to reduce the activity of pathogen by specific mechanism of action were identified and tested for their disease suppressive effects. After in vitro compatibility examinations, 21 individual strains and 15 combinations were tested in the greenhouse. Results indicated three-way combinations of different mode of control, TS3-7+A253-16+SKU78 and TS1-5+A100-1+SKU78, enhanced disease suppression by 70%, as compared to 30-50% reduction for their individual treatments. This work suggests that combining multiple traits antagonizing the pathogen improve efficacy of the biocontrol agents against Ralstonia solanacearum.

• Journal of Ginseng Research
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• v.33 no.4
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• pp.355-360
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• 2009

Phenanthrene ($C_{14}H_{10}$) is a polycyclic aromatic hydrocarbon with three aromatic rings, and it can be produced by incomplete combustion of fossil fuels. Comet assay was used to examine the oxidative DNA damage of lymphocytes by phenanthrene and to measure the suppressive effects of ginseng extract on the DNA damage in this investigation. The in vitro oxidative DNA damage by phenanthrene increased in a dose-dependent manner in the lymphocyte. However, the DNA damage was significantly inhibited by ascorbate. Moreover, pretreatment, cotreatment and posttreatment with ginseng extract enhanced lymphocyte resistance to the phenanthrene-induced DNA damage. Phenanthrene enhanced the generation of intracellular reactive oxygen species, and the elevated reactive oxygen species level was reduced by treatment with ginseng extract.