• 한국응용곤충학회지
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• 제60권2호
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• pp.229-234
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• 2021

본 연구에서는 멀티콥터를 이용한 무인항공용 방제약제 살포시 사용하는 상용 노즐에 3가지 타입에 따른 살포양상과 피복도를 조사하였다. 처리구에 플루페녹수론·메타플루미존(flufenoxuron+metaflumizone) 액상수화제와 비펜트린(bifenthrin) 유제를 살포하고난 뒤, 살포 약제의 낙하입자를 감수지를 이용하여 측정하고 감수지의 피복도를 조사하였다. 낙하입자 분석결과 풍속의 차이에 따라 노즐별 낙하입자 균일도에 차이를 보였으며 제형별 차이는 없었다. 노즐별 분사량은 공식 제공정보와 실제 분사량에 차이를 보였다. 이 결과들은 무인항공기 이용 농업현장방제 및 약제등록시험 가이드라인 설정에 근거자료로 활용될 수 있을 것이다.

• Archives of Plastic Surgery
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• 제33권3호
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• pp.388-391
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• 2006

Hemangioma is one of the most common congenital tumors in the region of the face and neck. Although histologically benign, these facial masses are clinically malignant for their deforming and inexorable growth, especially in so-called 'cavernous hemangioma'. Carvenous hemangioma is the most common primary tumor occurring in the adult orbit. This tumor has symptoms that characteristically develop over several years with slowly progressive proptosis, eyeball deviation, hyperopia, diplopia and optic nerve compression. Today, hemangiomas are being treated by various methods; steroids, electrocoagulation, injection of sclerosing agent, cryotherapy, radiation therapy, laser therapy, and surgical treatment, etc. In principle, surgical approaches to the orbit must provide maximum safety and optimal visualization. We have experienced a case of large cavernous hemangioma in the orbit inferolaterally. The surgical treatment of tumor was achieved by the bicoronal approach combined with inferomedial and inferolateral orbitotomy. This surgical approach allows better visualization of the tumor and greater protection of essential anatomic structures. We obtained satisfactory results in terms of aesthetic and functional consideration. We present our case with a brief review of the literature related to orbital cavernous hemangioma.

• Journal of Microbiology and Biotechnology
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• 제26권12호
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• pp.2138-2140
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• 2016

The goal of this study was to identify a source of natural plant compounds with inhibitory activity against pepper mild mottle virus (PMMoV). We showed, using a half-leaf assay, that murrayafoline-A (1) and isomahanine (2) isolated from the aerial parts of Glycosmis stenocarpa have inhibitory activity against PMMoV through curative, inactivation, and protection effects. Using a leaf-disk assay, we confirmed that 2 inhibited virus replication in Nicotiana benthamiana. Using electron microscopy, we found that a mixture of the virus with 2 resulted in damage to the rod-shaped virus.

• 생약학회지
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• 제49권1호
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• pp.7-14
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• 2018

We isolated gallotannin, 2,6-digalloyl-1,5-anhydroglucitol, known as acertannin (1), from the barks and xylems of Acer ginnala Maxim. It is a genus of Acer species of shrubs in the family Aceraceae. A. ginnala grows in Korea, Japan and Mongolia. We accomplished the structure elucidation by confirming that the result of $^1H$,$^{13}C-NMR$,MS spectrum data was similar to previous references. We measured DPPH and ABTS radical scavenging activity in vitro to evaluate anti-oxidative activities on acertannin isolated from A. ginnala. Acertannin from A. ginnala exhibited potent DPPH and ABTS radical scavenging activities. We examined the antioxidant and apoptosis modulative effects. This examination shows that A. ginnala has not only 1,1-diphenyl-2-picryhydrazyl(DPPH) radical scavenging activity and ABTS radical scavenging activity, but also human hair dermal papilla cell protection effects. These results indicate that the barks and xylems of A. ginnala might be developed as a potent anti-oxidant, hair growth agent, and ingredient for related new functional cosmetic materials.

• 한국미생물·생명공학회지
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• 제49권4호
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• pp.528-533
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• 2021

The microalga, Tetrathelmis tetrathele, is used in the development of products for the aquaculture, food, and nutraceutical industries. In the present study, we investigated whether the T. tetrathele ethanolic extract (TTE), which has anti-inflammatory properties, can confer protection against alopecia and improve scalp health, influence the proliferation of human keratinocytes, HaCaT cells, and human hair follicle dermal papilla cells (HFDPC), or inhibit 5α-reductase activity. We found that TTE inhibited the production of the inflammatory mediator, nitric oxide (NO), and prostaglandin E₂ (PGE₂) without cytotoxicity in LPS-stimulated RAW 264.7 cells. In addition, TTE encouraged the proliferation of HaCaT cells and HFDPC. Our results showed that TTE had anti-inflammatory activities, proliferated HaCaT cells and HFDPC, and inhibited 5α-reductase activity. Therefore, we suggest that T. tetrathele could be a potent therapeutic agent for alopecia prevention and scalp improvement.

• 한국환경보건학회지
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• 제47권5호
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• pp.432-445
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• 2021

Background: The European Centre for Ecotoxicology and Toxicology of Chemicals' Targeted Risk Assessment (ECETOC TRA) tool has been recognized by EU REACH as a preferred approach for calculating worker health risks from chemicals. Objectives: The applicability of the ECETOC TRA to occupational exposure estimation from industrial uses of methanol was studied by inputting surveyed and varied parameters for TRA estimation as well as through comparison with measured data. Methods: Information on uses of methanol was collected from seven working environment monitoring reports along with the measured exposure data. Input parameters for TRA estimation such as operating conditions (OCs), risk management measures (RMMs) and process categories (PROCs) were surveyed. To compare with measured exposures, parameters from the surveyed conditions of ventilation but no use of respiratory protection were applied. Results: PROCs 4, 5, 8a, 10, and 15 were assigned to ten uses of methanol. The uses include as a solvent for manufacturing sun cream, surfactants, dyestuffs, films and adhesives. Methanol was also used as a component in a release agent, hardening media and mold wash for cast products as well as a component of hard-coating solution and a viscosity-controlling agent for manufacturing glass lenses. PROC 8a and PROC 10 of a cast product manufacturer without LEV (local exhaust ventilation) and general ventilation as well as no respiratory protection resulted in the highest exposure to methanol. Assuming the identical worst OCs and RMMs for all uses, exposures from PROC 5, 8a, and 10 were the same and the highest followed by PROC 4 and 15. The estimation resulted in higher exposures in nine uses except one use where measured exposure approximated exposures without RMMs. Conclusions: The role of ECETOC TRA as a conservative exposure assessment tool was confirmed by comparison with measured data. Moreover, it can guide which RMMs should be applied for the safe use of methanol.

• 패션비즈니스
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• 제10권1호
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• pp.120-131
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• 2006

Recently, it has been reported that chemical hair coloring can cause allergic reactions, the toxicity issue of chemical hair color was issued. Therefore, there is an increasing interest in the hair coloration technique using non-toxic and bio-compatible hair color gotten from natural resource. In this study, the possibility using fermented Indigo as natural hair coloring agent was investigated. Bleached hair samples were dyed using Indigo, and the effect of dyeing frequency, the physical change after dyeing, color, surface and cross-section characteristics, and tensile property were also studied. In addition, the protection property of cationic detergent finishing on damaged hair samples were also studied. The following conclusions are made; 1. While bleached hair samples with low value showed the negligible effect of repeated dyeing, bleached hair samples with high value showed the good effect of repeated dyeing. 2. Hair sample dyed with Indigo showed a high light-fastness, however, tensile strength and elongation showed very low values (high damage on hair sample). 3. Cationic detergent finishing did not affect on the colorfastness because of decoloration after dyeing, however, it increased the smoothness of hair sample, and therefore, can use as protectant of damaged hair. 4. Hair scale was damaged after dyeing. It seemed that the alkali, potassium carbonate, increased pH of dyeing bath to 11, as a result, the hair was swollen, weaken, and dissolved on the prolonged dyeing time. Dyed hair sample became stiff and fine. 5. The color difference was 4.62 (a high fastness value) in the test of sunlight exposure, shampoo, cationic detergent finishing, and acid perspiration fastness.

• Journal of Forest and Environmental Science
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• 제31권4호
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• pp.312-316
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• 2015

Rhizina undulata, the causal agent of Rhizina root rot, is a soil-borne fungus occurring on coniferous trees. The destruction of coastal forests caused by R. undulata infection has been mainly associated with bonfires at camping sites. However, Rhizina root rot was observed in the western coastal forests without fire. It was hypothesized that Rhizina root rot in this area might be closely related to the soil salinity, which can facilitate the growth and survival of R. undulata. So, the variation in sodium chloride (NaCl) resistance among isolates of Rhizina undulata was compared using liquid media containing different concentrations of NaCl ranging from 0 mM to 300 mM. Our results showed that, albeit of no growth at a higher concentration of NaCl (300 mM), most of R. undulata isolates were capable of germinating and grew at up to 100 mM, indicating that NaCl resistance varies among R. undulata isolates. It was further found that isolates from coastal areas seemed to be more tolerant to NaCl than those further away the coast. We demonstrated that R. undulata could be possible to survive in coastal areas, but was lower NaCl tolerance than other fungi.

• 한국토양비료학회지
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• 제44권4호
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• pp.650-656
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• 2011

Lysobacter antibioticus HS124 isolated from pepper rhizosphere soil produced catechol type siderophore. Purified siderophore by Diaion HP-20 and silica gel column chromatography showed several hydroxyl functional groups adjacent to benzene rings by analysis of $^1H$ NMR spectroscopy. The strain HS124 showed different activities to suppress Phytophthora capsici with different concentrations of exogenous Fe (III) in minimal medium where antifungal activity with $100{\mu}M$ Fe (III) was approximately 1.5 times higher than in absence of Fe (III). Bacterial population in this Fe (III)-amended medium was also highest with $8.9{\times}10^8\;CFU\;ml^{-1}$ which also corresponded to the strongest siderophore activity. When grown in rich medium (minimal medium with N, $P_2O_5K_2O$ and glucose), HS124 exhibited approximately 2 times stronger antifungal activity compared to minimal medium. In pot trials, treatments of bacterial culture grown in rich medium with (C1) or without (C2) $100{\mu}M$ Fe (III) exhibited a high protection of pepper plants from disease, compared to medium only with (M1) or without (M2) $100{\mu}M$ Fe (III). Especially, treatment C1 showed the best disease control effect of about 70 %. Thus, the strain HS124 should be recommended as a potential biocontrol agent against P. capsici in pepper.

• Journal of Plant Biotechnology
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• 제38권3호
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• pp.209-214
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• 2011

본 실험에서는 상추 연산홍에 apoptosis 관련 유전자인 Bcl-2 유전자를 도입하여 내병성 상추의 육성을 하기 위한 목적으로 형질전환을 수행하였다. 상추의 자엽조직을 NPTII-35S Promoter와 Bcl-2 유전자가 삽입된 Agrobacterium GV 3101과 공동배양 한 후 0.1 mg/L NAA, 0.5 mg/L BAP, 100 mg/L Kanamycin, 300 mg/L Lilacillin이 첨가된 MS 배지에서 식물체가 유기되었다. Kanamycin 내성을 가진 식물체들을 PCR, Southern blot 분석을 통해 Bcl-2 유전자가 안정적으로 식물체 genome 안에 삽입되었음을 확인하였다. 100개의 형질전환식물체가 확인되었으며 T1식물체를 채종하였다. T1 종자를 파종하여 Sclerotinia sclerotiorum. 균주를 접종하여 내병성 검정을 실시하였고 그 중 2개의 line에서 내병성을 확인하였다. 이러한 결과를 통하여 인간의 apoptosis에 관련하는 유전자가 식물체 내에서 안정된 발현을 통하여 내병성을 증가시켰음을 밝혔다.