• Title/Summary/Keyword: sulfated polysaccharides

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Flow Properties of Sulfated Polysaccharides in the Tunic of the Ascidin Halocynthia roretzi (우렁쉥이 껍질 유래 황산다당의 유동 특성)

  • Son, Byoung-Yil;Hong, Byeong-Il;Ahn, Jong-Woong
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.40 no.2
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    • pp.68-72
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    • 2007
  • The flow properties of sulfated polysaccharides purified from the ascidian Halocynthia roretzi tunic were investigated. The sulfated polysaccfarides produced aqueous solutions of low apparent viscosity with pseudoplastic flow behavior. The respective activation energies of TCA-treated and TCA-nontreated solutions were $2.2248{\times}10^4\;and\;1.442{\times}10^4J/kg{\cdot}mol$ at a 150L/s shear rate. The viscosity of the sulfated polysaccharides solution was increased by the addition of sugar, while it was not changed by the addition of NaCl.

Immuno-stimulatory Effects of Sulfated Polysaccharides Isolated from Codium fragile on Interleukin-1β Gene Expression in Olive Flounder, Paralichythys olivaceus

  • Yang, Yong;You, Sang Guan;Hong, Suhee
    • Journal of Marine Life Science
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    • v.2 no.1
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    • pp.7-11
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    • 2017
  • Sulfated polysaccharides are known to be immune-stimulators in mammals and can be used as food additives to enhance immunity. In this study, the immune-stimulating activity of water-soluble anionic macromolecules F2 fractionation isolated from Codium fragile using ion-exchange chromatography was tested in olive flounder, Paralichythys olivaceus, in vitro and in vivo. The gene expression of interleukin (IL)-1β was adopted to check the immune-affection. As a result, in vitro study revealed that the expression of IL-1β was significantly upregulated in head kidney cells by 1 and 5 ㎍/ml of polysaccharides 4 h and by 5 ㎍/ml of polysaccharides at 24 h. In vivo, IL-1β gene expression in head kidney was significantly upregulated by 20 and 100 ㎍ of the polysaccharides at day 1 post-i.p. injection, while downregulated at day 3 but not significant. Meanwhile, in peritoneal cells, it was upregulated by 20 ㎍ of the polysaccharides at day 1 but the upregulation was sustained until day 3 though it was not significant. These results indicate that the sulfated polysaccharides from C. fragile are an immune-stimulator and might be potential feed additives for olive flounder.

Mechanism of Lipid Accumulation in Atherosclerosis (동맥경화증(動脈硬化症)의 지방축적(脂肪蓄積) Mechanism)

  • Kim, Young-Choong
    • Korean Journal of Pharmacognosy
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    • v.9 no.4
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    • pp.157-167
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    • 1978
  • Atherosclerosis is associated with the presence of extracellular lipid droplets and large fatty deposits, both of which are to be covered at the surface mainly by zwitterionic phospholipids. The development of atherosclerosis is often associated with the accumulation of calcium. Furthermore, the presence of glycosaminoglycans directly underlying fatty deposits in human aorta has been demonstrated. Also, the possible involvement of the interaction between sulfated polysaccharide and lipoprotein in the development of atherosclerosis has been suggested in view of the presence of both low density lipoproteins and glycosaminoglycans, as well as their complexes, in atherosclerotic aortas. Therefore interactions of sulfated polysaccharides with low density lipoproteins which serve as a vehicle for cholesterol and cholesterol ester and with zwitterionic phospholipids have been studied extensively by a number of workers to provide mechanisms. In this paper, the mechanism of the interaction of sulfated polysaccharides with low density lipoproteins and the mechanism of the interaction between sulfated polysaccharides and zwitterionic phospholipids are reviewed. The possibility of the occurence of these interactions in the body are also considered.

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Preparation of Heparinoids from Acidic Plant Polysaccharides (식물성 산성당으로부터 헤파리노이드의 제조)

  • Kim, Yeong-Shik;Roh, Ji-Eun;Ann, Hyung-Soo;Park, Ho-Koon
    • YAKHAK HOEJI
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    • v.36 no.4
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    • pp.350-356
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    • 1992
  • Anticoagulant activities were tested for the fifteen kinds of medicinal plants by measuring activated partial thromboplastin time (aPTT). Of them five kinds or species (Artemisia princeps, Sanguisorba officinalis, Artemisia apiacea, Eclipa alba, Schizonepeta tenuifolia) were selected and fractionated for the preparation of acidic polysaccharides. They were extracted with water by refluxing and the extracts were precipitated with ethanol. The precipitates were separated based on charge using a DEAE-Sephadex. The low salt and high salt fractions were sulfated with anhydrous pyridine and chlorosulfonic acid complex. In vitro anticoagulant activities of sulfated polysaccharides were tested by measuring aPTT, prothrombin time (PT), and factor Xa clotting time using normal human plasma. No relationship was found between the amount of uronic acids and anticoagulant activities, but the sulfated ones show the increase of activities. In vivo anticoagulant properties of the sulfated polysaccharide from Artemisia apiacea were also tested by the intraveneous administration of three different doses (3,5 and 10 mg/kg) to rats. APTT and PT were increased significantly and the action of factor Xa and thrombin mediated through antithrombin III were inhibited slightly.

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Anticancer Activity of Sulfated Polysaccharides Isolated from the Antarctic Red Seaweed Iridaea cordata

  • Kim, Hak Jun;Kim, Woo Jung;Koo, Bon-Won;Kim, Dong-Woo;Lee, Jun Hyuck;Nugroho, Wahyu Sri Kunto
    • Ocean and Polar Research
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    • v.38 no.2
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    • pp.129-137
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    • 2016
  • This study aimed to isolate and characterize sulfated polysaccharides (SPs) from Iridaea cordata and evaluate their anticancer activity. SPs of the Antarctic red seaweed were obtained by $CaCl_2$ (SP1) and ethanol precipitations (SP2) following diluted acid extraction at room temperature. Yields of SP1 and SP2 were approximately 14% and 23%, respectively, of the dry weight of red seaweed. The average molecular mass of the SP1 and SP2 was estimated about $1.84{\times}10^3$ and $1.42{\times}10^3kDa$, respectively, by size-fractionation High-Performance Liquid Chromatography (HPLC). From the High-Performance Anion-Exchange Chromatography-Pulsed Amperometric Detection (HPAEC-PAD) analysis, the main monosaccharide was galactose with glucose and fucose as minor components. The sulfate content of SP2 (40.4%) was slightly higher than that of SP1 (33.8%). The FT-IR spectra also showed characteristic band of carrageenan-like sulfated polysaccharides. Taken together the SPs are thought to be carrageenan-like sulfated galactan. The polysaccharides (SPs) from I. cordata exhibited weak antitumor activity against PC-3 (prostate cancer), HeLa (cervical cancer), and HT-29 (human colon adenocarcinoma). To our knowledge, this is the first data on biological activity of the Antarctic red seaweed I. cordata.

Functions and values of sulfated polysaccharides from seaweed

  • D. P. Nagahawatta;N. M. Liyanage;Thilina U. Jayawardena;Fengqi Yang;H. H. A. C. K. Jayawardena;M. J. M. S. Kurera;Fahe Wang;Xiaoting Fu;You-Jin Jeon
    • ALGAE
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    • v.38 no.4
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    • pp.217-240
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    • 2023
  • Sulfated polysaccharides (SPs) isolated from seaweed have emerged as remarkable bioactive compounds with a wide spectrum of biological activities and have substantial value in the scientific and industrial domains. The current study explores the diverse biological activities of SPs and their relationship with their structures. This aids in an in-depth examination of the multifaceted biological activities of SPs, including anticoagulant, anti-inflammatory, antiviral, antioxidant, and immunomodulatory properties, which underpin their potential health benefits. Furthermore, the current study explores the complicated properties of SPs, with their extraction methodologies and techniques for precise characterization. Elucidation of the commercial significance of SPs derived from brown, red, and green seaweed by highlighting their potential applications has emphasized their importance in human well-being. Further, this review emphasizes the challenges needed to overcome research and industrial innovations for SPs. Collaboration among researchers, industry stakeholders, and regulatory authorities can overcome these challenges and elevate the potential of SPs to revolutionize industries such as pharmaceuticals, cosmeceuticals, food, and biotechnology.

Functional Properties of Sulfated Polysaccharides in Ascidian(Halocynthia roretzs) Tunic (우렁쉥이 껍질 중 황산화다당의 기능적 특성)

  • LEE Kang-Ho;CHOI Byeong-Dae;HONG Byeong-Il;JUNG Byung-Chun;RUCK Ji-Hee;JUNG Woo-Jin
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.31 no.3
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    • pp.447-451
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    • 1998
  • Functional properties such as anti-blood coagulation, angiotensin converting enzyme (ACE) inhibitory activity, fat binding capacity, foaming properties, emulsifying properties and chemical components of sulfated polysaccharides isolated from ascidian tunics were investigated. The sulfated polysaccharide mainly consisted of sulfate, uronic acid, protein, and chondroitin sulfate, among which chondroitin sulfate showed higher concentration while sulfate and uronic acid did lower. Compositional menosaccharides were arabinose, xylose, glucose, galactose, glucuronic acid, N-acetylgalactosamine and N-acetyglucosamine. Especially, galactose content was dominant among them. And emulsifiability and foaminess of the sulfated polysaccharide was higher than the control group. Anti-blood coagulation of sulfated polysaccharide showed with respect to APTT (Activated partial thromboplastin time). ACE inhibitory activity showed about $16.7\%$.

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Utilization of Pigments and Tunic Components of Ascidian as an Improved Feed Aids for Aquaculture 2. Chemical Properties of Sulfated Polysaccharides in Ascidian (Halocynthia roretzi) Tunic (우렁쉥이 껍질성분 및 색소를 이용한 양식소재 개발 2. 우렁쉥이 껍질 중 부분정제한 황산다당의 화학적 특성)

  • HONG Byeong-Il;JUNG Byung-Chun;JUNG Woo-Jin;RUCK Ji-Hee;CHOI Byeong-Dae;LEE Kang-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.34 no.6
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    • pp.632-637
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    • 2001
  • Components of polysaccharides isolated from ascidian tunic were measuerd by gel filtration, electrophoresis and chemical analyses. The sulfated polysaccharides consisted in sulfate, protein, uronic acid and amino sugars. Hexosamines were composed of arabinose, xylose, glucose, galactose, glucuronic acid, N-acetylgalactosamine and N-acetylglucosamine by gas chromatography analysis. The galactose was predominant hexose after autoclave and nutrase digestion followed by DEAE-cellulose ion exchange chromatography and gel-permeation chromatography on Sephadex G-100 and G-25. FT-IR spectra of isolated polysaccharides from ascidian tunic and standard chondroitin sulfates have similar functional groups of the type of vibration and frequency. Molecular weight of isolated polysaccharides by autoclave represented more than 40 kDa by polyacrylamide gel electrophoresis. But the neutrase treatment group divided into three band. The highest molecular band group was shown more than 100 kDa, and the two low molecular band group were shown about 22 kDa and 5 kDa, respectively, compare to standard materials.

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Utilization of Pigments and Tunic Components of Ascidian as an Improved Feed Aids for Aquaculture -3. Functional Properties of Sulfated Polysaccharides from Ascidian (Halocynthia roretzi) Tunic- (우렁쉥이 껍질성분 및 색소를 이용한 양식소재 개발 -3. 우렁쉥이 껍질 유래 황산다당의 기능적 특성-)

  • Hong Byeong Il;Jung Byung Chun;Son Byung Yil;Jung Woo Jin;Ruck Ji Hee;Choi Byeong Dae;Lee Kang-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.35 no.6
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    • pp.671-675
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    • 2002
  • The present study was conducted to elucidate functional properties of sulfated polysaccharides from ascidian tunics, In physical properties of the crude polysaccharides, emulsion ability and foaminess were more excellent compared with chitin and chitosan, particular dye binding capacity was prominent. Anti-blood coagulation of partially purified sulfnted polysaccharides showed with respect to APTT (Activated partial thromboplastin time). Especially, active fraction $(F_4)$ obtained by DEAE-cellulose ion exchange chromatography showed highest activity, which was approximately $20\%$ of the activity of heparin. ACE inhibitory activity also similar to anticoagulant activity. Active fraction $(F_4)$ obtained by DEAE-cellulose ion exchange chromatography showed about $34\%$, in ACE inhibitory activity.