• 한국식품과학회지
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• 제40권4호
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• pp.375-381
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• 2008

식품에 사용이 금지된 허용 외 색소 중 Orange II, Rhodamine B, Para red, Sudan I-IV 색소의 효율적인 수입식품 안전관리와 수용성 착색료 및 지용성 착색료로 이원화되어 있는 허용 외 착색료의 신속하고 정확한 분석을 위하여 동시 분석법을 개발하고자 하였다. 문헌조사를 바탕으로 분석을 위한 컬럼, 이동상 조건, 분석파장, 전처리 조건 등을 검토하여 분석법을 개발하였으며, 개발된 분석법의 검출한계 설정, 유효성 검증 및 회수율을 검토하였다. 26가지 제품에 대한 회수율 검토결과 Orange II는 96.46-121.26%, Rhodamine B는 70.86-106.53%, Para red는 97.00-116.86%, Sudan I은 92.93-112.44%, Sudan II는 96.63-115.10%, Sudan III는 92.21-114.73%, Sudan IV는 93.22-122.91%의 회수율을 나타냈다. 본 분석법 검량선의 상관계수는 0.999 이상을 보였으며 정밀성의 RSD는 0.8-1.39%로서 모두 2 이하의 값을 보였으며, 정확성은 90% 이상을 나타냈다. 이때, 검출한계 및 정량한계는 각각 0.1 mg/L, 0.3 mg/L로 결정하였다.

• 펄프종이기술
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• 제47권5호
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• pp.33-42
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• 2015

Pitches contained in thermomechanical pulp negatively influence paper qaulity and pulp and papermaking process. Without controlling TMP pitches suitably, it is hard to make a certain quality of paper. In order to control pitches in TMP, they must be quantitatively recognized by proper tools. One of the most widely used way to detecting TMP pitches is a staining method using a hydrophobic dye such as Sudan IV. Sudan IV could be used with three different protocols including different application of its dissolution, washing, dyeing time, etc. The dyeing protocols were classified into three categories including Stain I, Stain II, and Stain III. In dyeing time, Stain I required more than 24 hours to dye pitches. On the other hand, Stain III could stain TMP pitches with the most brief way. The images of red-stained pitches could be captured by a stereomicroscope with ${\times}35$ and ${\times}45$ magnifications, and then quantitatively analysed measuring their numbers and areas by Carl Zeiss AxioVision (ver. 4.8.2) program. Among three protocols, both Stain I and Stain III were the most ideal methods to detect TMP pitches because they detected more pitches and bigger pitch areas compared to Stain II against the same specimen. In particular, it was recognized that Stain III could be used as the most useful tool to detect TMP pitches accurately within several minutes.