• Parasites, Hosts and Diseases
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• 제55권3호
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• pp.341-345
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• 2017

The metacercariae of Artyfechinostomum malayanum (Leiper, 1911) Mendheim, 1943 were discovered in Pila sp. snails purchased from a market in Phnom Penh, Cambodia. They were isolated from the snails using the artificial digestion technique and were orally fed to 2 hamsters, 1 rat, and 2 mice to obtain the adult flukes. The metacercariae were round, $145-165{\mu}m$ in diameter, having a cyst wall of $6-10{\mu}m$ in thickness, a head collar and collar spines, and characteristic features of excretory granules. Adult flukes were recovered in the small intestines of the animals at days 14 and 32 post infection and were morphologically observed using a light microscope and a scanning electron microscope. They were plump or elongated, ventrally curved, $6.0-8.1{\times}1.6-2.0mm$ in size, and characterized by the head collar bearing 43 collar spines, including 5 end group ones on each side, a long cirrus sac extending beyond the posterior margin of the ventral sucker, a submedian ovary, and 2 deeply lobed testes. Eggs in uteri were operculate, ovoid to ellipsoid, and $120-135{\times}68-75{\mu}m$ in size. In scanning electron microscopy, the head collar was prominent with collar spines looking like horns. Scale-like tegumental spines were densely distributed on the ventral surface between the head collar and ventral sucker. Sensory papillae were distributed mainly on the tegument around suckers. By this study, it has been first confirmed that the life cycle of A. malayanum exists in Cambodia.

• Parasites, Hosts and Diseases
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• 제48권4호
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• pp.335-338
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• 2010

Maritrema spp. (Digenea: Microphallidae) are parasites of birds, but have not been found in the Republic of Korea. In this study, metacercariae of Maritrema sp. were discovered in the Asian shore crab, Hemigrapsus sanguineus, caught in the mud-flats of Jebu-do, Hwasung-gun, Gyeonggi-do, and the adult flukes were confirmed by experimental infection into mice. Based on the symmetric ribbon-like vitellarium, adult flukes of Maritrema sp. were identified, but did not belong to previously described species in terms of the following morphologic characteristics: ceca reaching to the lateral wall at the anterior border of the ovary; ventral sucker larger than oral sucker; a prominent metraterm; and vitellarium forming a complete ring. Hence, we named this microphallid M. jebuensis n. sp. after the island where the second intermediate hosts were collected. From this study, it has been shown that Maritrema sp. is distributed in Korea and transmitted by the Asian shore crab, H. sanguineus.

• Parasites, Hosts and Diseases
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• 제31권1호
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• pp.7-12
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• 1993

반지락(Tapes philippinarurm)에서의 수집한 피낭유충을 동정하기 위하여 ICR계 마우스에 피낭유충을 경구감염시킨 다음 제 7일, 제 10일, 제 14일에 희생시키고 소장을 적출하여 충체를 회수하였다. 그 결과는 다음과 같다. 1.피낭유충은 작은 충체($312{\;}{\times}{\;}202{;\}{\mu\textrm{m}}$)가 피포하지 않은 상태로 감염되어 있고, 구홉반이 크고 잘 발달되었고 측명돌기가 관찰되었다. 장관은 이분지 되어 끝부분이 매우 부풀어 오른 맹관이었으며 복흡반까지 닿아 있었다. Ventral pit는 관찰되지 않았고 생식공은 원형으로 구홉반과 복합반 사이에 위치하였다. 배설낭은 V자 모양으로 두팔부분은 구홉반까지 닿아 있었다. 2.마우스 소장에서의 회수율 평균 20.1% 이었으며 감염 후 10일 째의 회수율이 가장 높았고 14일에는 현저히 간소하였다. 3.성충은 피낭유충과 크기의 차이가 없었다. 난황선은 한 개가 복합반의 왼쪽 옆에 위치하였다. 저장낭은 한부분으로 이루어져 있었고 복흡반보다 전방에 위치하였다. 고환은 한쌍으로 복흡반 후측장에 위치하였고 난소는 오른쪽 고환이 전방에 위치하였다. 충란은 길이 $28{;\}{\mu\textrm{m}}(27-30{;\}{\mu\textrm{m}})$, 폭 $19{;\}{\mu\textrm{m}}(17-20{;\}{\mu\textrm{m}})$으로 껍질이 매우 얇았으며 날개가 있었다. 이상의 결과로 반지락을 제2중간숙주로 하는 Parvatrema timondavidi(Digenea:Gymnophallidae)가 국내에 분포함을 확인하였다.

• Parasites, Hosts and Diseases
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• 제48권3호
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• pp.271-274
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• 2010

Adult worms of Parvatrema spp. (Digenea: Gymnophallidae) were found in the intestines of 2 species of migratory birds, i.e., a great knot, Calidris tenuirostris, and 2 Mongolian plovers, Charadrius mongolus, in the coastal area of Gunsan-si, Jeollabuk-do in October 2009. The recovered Parvatrema worms were 79 in total number and composed of 2 species. The worms from a great knot were $289{\mu}m$ in length with the oral and ventral sucker ratio of 2:1. They had a single vitellarium, and their intrauterine eggs were $25.0{\times}17.5{\mu}m$ in size. These findings were compatible with P. duboisi (Dollfus, 1923) Bartoli, 1974 (syn. P. timondavidi Bartoli, 1963). The worms recovered from the Mongolian plovers were smaller in length than P. duboisi and had 2 vitellaria. The oral and ventral sucker ratio was 2.5 : 1, and the eggs were $17.5{\times}8.8{\mu}m$ in size. These worms were assigned to be P. homoeotecnum James, 1964. This is the first report on the natural final hosts of Parvatrema spp. in Korea.

• Parasites, Hosts and Diseases
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• 제47권1호
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• pp.25-29
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• 2009

Paragonimus harinasutai metacercariae were found in a species of freshwater crab, Indochinamon ou, collected in a small stream of Namback District, Luang Prabang Province, Lao PDR. Adult flukes were recovered after experimental infection of the metacercariae to dogs. Metacercariae were round or slightly elliptical, $0.666{\times}0.626\;mm$ in average size, and had a thin cyst wall of about $20{\mu}m$ in thickness, a black excretory bladder, convoluted ceca, and some pinkish materials in the body. Adults were somewhat elongated, $95.2{\times}36.5\;mm$ in average size, covered with single-tipped tegumental spines, had a smaller oral sucker than the ventral sucker, a moderately branched ovary, and 5-6 lobulated testes. Eggs were ovoid and bilaterally symmetrical in shape, $79{\times}45{\mu}m$ in average size, and had a uniformly thickened shell. By the present study, it has been confirmed that I. ou is a new second intermediate host for P. harinasutai.

• 한국어병학회지
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• 제25권2호
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• pp.85-93
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• 2012

노지 양식장에서 비단잉어와 함께 사육중인 참붕어의 미병부, 체측근, 복부 및 아가미 뚜껑 내부에 황백색의 cyst내의 metacercaria를 탈낭시켜 형태학적 및 분자생물학적으로 조사한 결과 Clinostomum complanatum으로 동정되었다. 병리조직학적으로는 상피세포의 박리와 괴사 및 cyst주변의 근섬유의 위축이외에 다른 조직의 변화는 발견되지 않았다.

• Parasites, Hosts and Diseases
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• 제45권2호
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• pp.115-120
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• 2007

Parvatrema chaii n. sp. (Digenea: Gymnophallidae) is described using the worms recovered from experimentally infected mice in Korea. The metacercariae were collected from surf-clams, Mactra veneriformis, from a tidal flat in Sochon-gun, Chungchongnam-do. The metacercariae were elliptical ($0.262{\times}0.132 mm$), and the genital pore had an anterior arch of 16-17 sensory papillae in scanning electron microscopic view, Adult worms were ovoid to foliate (0.275-0.303 by 0.140-0.150 mm), and their characteristic features included the presence of lateral lips, short esophagus, genital pore located some distance anterior to the ventral sucker, club-shaped seminal vesicle, a compact to slightly lobed vitellarium, elliptical eggs (0.018-0.020 by 0.010-0.013 mm), and absence of the ventral pit. This gymnophallid is classified as a member of the genus Parvatrema because of the location of the wide genital pore some distance from the ventral sucker, and the absence of the ventral pit. It differs from previously reported Parvatrema species, including the type species, P. borinquenae. In particular, the morphologies of the vitellarium and the genital pore with an anterior arch of 16-17 sensory papillae are unique features. Therefore, we propose it as a new species, Parvatrema chaii n. sp. (Digenea: Gymnophallidae).

• 한국임상수의학회지
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• 제36권6호
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• pp.330-335
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• 2019

Apharyngostrigea ibis (Trematoda: Strigeidae) were obtained from the small intestine of two black-crowned night herons (Nycticorax nycticorax) and three grey herons (Ardea cinerea jouyi) between July 2006 and September 2018 in Daejeon metropolitan city and Kangwon province, Republic of Korea. The body is covered with a relatively thick cuticle, devoid of spines, and bipartite. The body measures at 2.3-3.33 × 0.86-1.20 mm, and the fore-body is ventrally concave. The oral sucker leads into a short esophagus that is devoid of a muscular pharynx. The worm was studied with a scanning electron microscope for the first time resulting in the detection of a pair of lappets located at the bilateral portion of the oral sucker. This is the first record of A. ibis in the Republic of Korea.

• Parasites, Hosts and Diseases
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• 제58권5호
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• pp.499-511
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• 2020

Echinostome metacercariae were investigated in freshwater snails from 26 districts in 7 provinces of upper northern Thailand. The species identification was carried out based on the morphologies of the metacercariae and adult flukes harvested from experimental hamsters, and on nucleotide sequences of internal transcribed spacer 2 (ITS2) and nicotinamide adenine dinucleotide dehydrogenase subunit 1 (nad1) genes. Twenty-four out of 26 districts were found to be infected with echinostome metacercariae in freshwater snails with the prevalence of 40.4%. The metacercariae were found in all 6 species of snails, including Filopaludina martensi martensi (21.9%), Filopaludina doliaris (50.8%), F. sumatrensis polygramma (61.3%), Bithynia siamensis siamensis (14.5%), Bithynia pulchella (38.0%), and Anenthome helena (4.9%). The echinostome metacercariae found in these snails were identified as Echinostoma revolutum (37-collar-spined) and Echinostoma macrorchis (45-collar-spined) morphologically and molecularly. The 2-week-old adult flukes of E. revolutum revealed unique features of the cirrus sac extending to middle of the ventral sucker and smooth testes. E. macrorchis adults revealed the cirrus sac close to the right lateral margin of the ventral sucker and 2 large and elliptical testes with slight indentations and pointed posterior end of the posterior testis. The ITS2 and nad1 sequences confirmed the species identification of E. revolutum, and the sequences of E. macrorchis have been deposited for the first time in GenBank. The presence of the life cycle of E. macrorchis is a new record in Thailand and the snail F. doliaris as their second intermediate host seems to be new among the literature.

• 문화기술의 융합
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• 제8권5호
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• pp.749-754
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• 2022

다양한 의료 응용 분야에서 웨어러블 및 피부 부착형 전자 패치에 피부 표면의 높은 접착력과 내수성이 요구된다. 본 연구에서는 탄소 기반 전도성 고분자 복합 소재에 개구리 발바닥의 육각 채널와 문어 빨판의 흡착 구조 패턴을 모사한 신축성 있는 전자 패치를 보고한다. 개구리의 발바닥을 모사한 육각 채널 구조는 수분을 배수하며, 균열억제 효과를 통해 점착력을 향상 시키며, 문어 빨판을 모사한 흡착 구조는 젖은 표면에서 높은 점착력을 나타낸다. 또한 고점착 전자패치는 실리콘(max. 4.06 N/cm²), 피부 복제 표면(max. 1.84 N/cm²) 등 다양한 표면에 건조 및 젖은 조건에서 우수한 접착력을 가지고 있다. 고분자 매트릭스와 탄소 입자를 기반으로한 고분자 복합소재를 통해 제작된 고점착 전자 패치는 건조 및 습한 환경에서 심전도(ECG)을 안정적으로 감지할 수 있다. 이 연구에서 보여진 특성을 기반으로 제안된 전자 패치는 다양한 생체 신호의 진단을 위한 웨어러블 및 피부 부착 센서 디바이스를 구현하는 잠재적 응용 가능성을 제시한다.