• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2000년도 The 7th International Symposium
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• pp.19-21
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• 2000

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2000년도 제7차 국제 심포지움(생약자원개발에 관한연구) 및 추계정기 학술발표회 초록집
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• pp.2-4
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• 2000

• Applied Microscopy
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• 제26권4호
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• pp.457-463
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• 1996

Water storage cells (WSCs) in the leaf succulent Orostachys malacophyllus have been studied to understand their adaptive nature to its coastal habitat employing the electron microscopy. Attention has been paid to the features of vacuoles and plasmodesmata in this study, since leaf tissues in O. malacaphyllus are under continous physiological drought due to its occurrence in the shore-line environment. The WSCs occupied almost all of the leaf volume and appeared empty at low magnifications. Among the WSCs, small rudimentary vascular bundles were scattered throughout the internal volume. However, in high magnification the WSCs were vacuolate in most cases and vacuolization into a well-developed huge central vacuole was very common phenomenon. Such vacuolization has been detected within the vacuoles as well as within the cytoplasms. Well-developed plasmodesmata were often found in cells appeared to be mucilagenous. Moreover, plasmodesmata being involved in the secretion of materials or structures were even encountered. Thus, vacuolization from various sizes of vacuoles in the WSCs to have a huge central vacuole seems playing an important role in adapting the plant itself to its coastal habitat.

• 인간식물환경학회지
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• 제22권2호
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• pp.159-165
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• 2019

The purpose of this study was to examine the effects of repetitive horticultural activities on the dexterity and out of seat behaviors of 5 years old children. Horticultural activities were conducted from March 3 to April 21, 2017, once a week for 20 minutes per session, a total of 8 sessions. The program was divided into first half (Sessions 1-4) and second half (Sessions 5-8) and horticultural activities were repeatedly carried out. In Sessions 1 and 5, kindergarteners transplanted succulent plants into pots, and observed and managed the plants daily. From Session 2 to 4 and from Session 6 to 8, kindergarteners watered and observed the succulent plants planted in Sessions 1 and 5 and they put flowers on a floral foam (10 × 10 × 10 cm). This study conducted the O'Connor Tweezer Dexterity Test in which the number of pins subjects inserted for 30 seconds was counted, and there was a significant difference (p =.000) in the dexterity of the kindergarteners after performing horticultural activities in the result of the repeated measures design ANOVA. The time kindergarteners left their seat for the first time while participating in horticultural activities for 20 minutes was measured using a stopwatch in each session. There was a significant difference (p =.000) in the ANOVA test in changes in their out of seat behavior in Session 8. In addition, there was no significant difference in the dexterity and out of seat behavior of kindergarteners from Session 2 (p =.364, p =.975, respectively) to Session 3 (p =.055, p =.283), but there was a significant difference from Session 4 (p =.042, p =.009) to Session 8 (p =.000, p =.000) as sessions were repeated.

• The Plant Pathology Journal
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• 제31권3호
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• pp.310-315
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• 2015

Certain bacterial species associate with plant roots in soil. The plant growth-promoting rhizobacteria (PGPR) stimulate plant growth and yield in greenhouse and field. Here, we examined whether application of known bacilli PGPR strains stimulated growth and asexual reproduction in the succulent plant Kalanchoe daigremontiana. Four PGPR strains B. amyloliquefaciens IN937a, B. cereus BS107, B. pumilus INR7, and B. subtilis GB03 were applied to young plantlets by soil-drenching, and plant growth and development was monitored for three months. Aerial growth was significantly stimulated in PGPR-inoculated plants, which was observed as increases in plant height, shoot weight, and stem width. The stimulated growth influenced plant development by increasing the total number of leaves per plant. Treatment with bacilli also increased the total root biomass compared with that of control plants, and led to a 2-fold increase in asexual reproduction and plantlet formation on the leaf. Collectively, our results firstly demonstrate that Bacillus spp. promote vegetative development of K. daigremontiana, and the enhanced growth stimulates asexual reproduction and plantlet formation.

• 환경위생공학
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• 제5권2호
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• pp.89-99
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• 1990

Morphology, anatomy and diurnal acid fluctuations were investigated monthly for the succulent C4 dicot Portulaca grandiflora Hook. growing under natural environmental conditions. Modified Kranz-Type sturcture and typical CAM-like cells were shown in leaves and stems, respectively. Values of indices of mesophyll succulence inleaves stems were 5.62 and 16.68, respectively. The number of stomata were 1476$\textrm{cm}^{-2}$ in leaves while the stomata in stems were not observed through growing seasons from spring to summer. However, on Sep. 16, 1981 in defoliate autumn season, stomata were begun to be produced for the first time in stems and the number were increased gradually to 216$\textrm{cm}^{-2}$ on Oct. 29. This feature can be interpreted as a seasonal plant dimorphism. P. grandiflora exhibited a diurnal fluctuation of titratable acidity in leaves and stems. Seasonal amplitudes of acid fluctuation in stems were as follows: 27 $\mu$eq./g.f.wt. on Sep. 21; 57$\mu$eq/g.f.wt. on Oct. 3; 80$\mu$eq./g.f.wt. on Oct. 21. Such the results in seasonal amplitude were able to consider due to decrease of light period and cool night air temperature according to change of season. Also, the naturally defoliated stems which had already stomata exhibited usual diurnal acid fluctuation, on the other hand the treated stems which possessed artificially closed stomata showed a few of acid fluctuation. Especially, stomata in stems are developed by seasonal dimorphism and activated stomata participate in CAM behavior of stems.

• Natural Product Sciences
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• 제9권4호
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• pp.270-272
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• 2003

The methanol extract of Euphorbia milii exhibited strong inhibitory effect on platelet aggregation in the cource of our search for anti-platelet component from succulent plants. Two components, components 1 and 2 were isolated from this plant. 1 was the mixture of 72% of 1-octacosanol (1a) and 28% of 1-triacontanol (1b), and 2 was identified as ${\beta}-sitosterol$. 2 ($IC_{50}$: $195\;{\mu}M$, and $170\;{\mu}M$ respectively) was about two fold stronger than ASA ($IC_{50}$: $420\;{\mu}M$ and $340\;{\mu}M$ respectively) on both collagen and U46619 induced aggregation, while the effect of 1 to platelets was negligible.

• Applied Microscopy
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• 제43권1호
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• pp.34-39
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• 2013

Glandular and nonglandular trichomes on the leaf surface of Plectranthus tomentosa were investigated by variable pressure field emission scanning electron microscopy (VP-FESEM). The segments of the plant's leaves were directly mounted without any specimen preparation, and examined at ambient temperature using a variable pressure secondary electron (SE) detector under ca. 15 Pa. Foliar trichomes maintained their shapes and structures without severe surface collapse or charging. The adaxial leaf surface was abundantly covered with different types of trichome. Nonglandular trichomes consisted of a basal cell and a long (up to ca. $300{\mu}m$) stalk. Meanwhile, capitate glandular trichomes had a secretory head and a short or long stalk. Peltate glandular trichomes with globose secretory heads were observed in close contact with the leaf epidermis. Spherical projections on the secretory head showed the secretion process of glandular trichomes. In addition to the trichomes, oval stomata were distributed on the abaxial leaf surface. These results suggest that ambient VP-FESEM can be used to classify the dehydration-sensitive foliar trichomes of succulent plants by SE imaging. At the FESEM resolution, this approach facilitates the rapid and detailed morphological analysis of a variety of trichomes in diverse plant taxa with reduced labor and preparation.

• 한국자원식물학회지
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• 제31권1호
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• pp.32-36
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• 2018

제주도 해안에서 선인장과의 한국 미기록 식물 Opuntia monacantha Haw.를 처음으로 발견하였다. "왕선인장"으로 명명된 O. monacantha Haw.(선인장과)는 곧추서서 자라며 상부 의가지들이 밑으로 쳐지는 경향이 있다. 그리고, 기존의 "선인장"에 비하여 식물체의 크기나 엽상경이 대형이다. 화피편 뒷면의 중륵에 자주색 줄무늬가 있다. 열매에는 엽침이 존재하지 않으며, 열매의 표면은 자주색을 띠나 과육은 황녹색이다. 종자는 대부분 흔적으로 남아있고 성숙한 종자는 매우 드물다.

• 한국약용작물학회지
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• 제25권2호
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• pp.95-101
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• 2017

Background: Callus cultivation has the advantage of producing a large amount of tissue of a plant in a laboratory regardless of the environment, for extracting an active substance. In the present study, callus formation was induced in the leaves of the succulent plant Adenium obesum (Forssk.) Roem & Schult. After callus cultivation, anti-inflammatory activity tests were conducted, because leaves and stems of A. obesum have been reported to possess biological activity. Methods and Results: In order to induce callus formation, various concentrations of plant growth factors, such as kinetin, naphtha-leneacetic acid (NAA), 6-benzyladenine (BA), and indole-3-acetic acid (IAA) were added to MS solid medium. The maximum callus proliferation was induced by mixed medium consisting of NAA ($2mg/{\ell}$) and BA ($1mg/{\ell}$). In addition, an elicitor was added to the medium under optimal conditions for initiating suspension culture. After suspension culturing, the activities of the callus extracts were compared and analyzed. The cytotoxicity and anti-inflammatory activity tests revealed that the anti-inflammatory activity of the callus extract and the content of phenolic compounds were elevated after treatment of the callus culture with the elicitior. Conclusions: A. obesum callus might be considered as potential source of biologically active anti-inflammatory material.