• Mycobiology
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• v.33 no.1
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• pp.19-22
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• 2005

We investigated the effect of nitrogen fixing Azotobacter bioinoculant on the mycelial growth and the rate of substrate utilization by Pleurotus eous. The synergistic or antagonistic role of the microorganism during dual culturing with the mushroom or the competitor molds Trichoderma viride, and Trichoderma reesi was studied. Azotobacter was inhibitory to the molds, which are competitive to the mushroom in the seed spawn substrate, but was synergistic towards the mushroom. The growth, substrate utilization potential as total nitrogen content and cellulase enzyme activities of the mushroom in the seed spawn substrate were also enhanced in the presence of the bioinoculant at lower inoculum concentrations, upto 5 ml broth culture per spawn bottle.

• Mycobiology
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• v.35 no.4
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• pp.196-204
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• 2007

The growth and bioconversion potential of selected strains growing on cassava waste substrate during solid state fermentation were assessed. Rhizopus stolonifer showed the highest and the fastest utilization of starch and cellulose in the cassava waste substrate. It showed 70% starch utilization and 81% cellulose utilization within eight days. The release of reducing sugars indicating the substrate saccharification or degradation potential of the organisms reached the highest value of 406.5 mg/g by R. stolonifer on cassava waste during the eighth day of fermentation. The protein content was gradually increased (89.4 mg/g) on the eighth day of fermentation in cassava waste by R. stolonifer. The cellulase and amylase activity is higher in R. stolonifer than A. niger and P. chrysosporium. The molecular mass of purified amylase and cellulase seemed to be 75 KDal, 85 KDal respectively.

• Journal of Mushroom
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• v.17 no.3
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• pp.85-92
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• 2019

Over a million tons of spent mushroom substrate (SMS) are generated as by-products of mushroom cultivation every year in Korea. Disposal of SMS by mushroom farmers is difficult, therefore, recycling solutions that do not harm the environment are necessary. SMS consists of mushroom mycelia and residues of fruiting bodies, containing a variety of bioactive substances, such as extracellular enzymes, antimicrobial compounds, and secondary metabolites. This paper reviews utility of SMS for bioremediation, controlling plant disease, and production of lignocellulytic enzymes, organic fertilizer, and animal feed.

• Journal of Microbiology and Biotechnology
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• v.25 no.6
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• pp.814-819
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• 2015

The natural crosslinking agent genipin has been applied widely in biomedicines and foods nowadays. Because of the special hemiacetal ring structure in its molecule, it can only be prepared by hydrolysis of geniposide according to biocatalysis. In this research, strategies including aqueous-organic biphasic catalysis and substrate fed-batch mode were adopted to improve the biocatalysis process of genipin. A 10 L ethyl acetate-aqueous biphasic system with geniposide fed-batch led to a satisfying genipin yield. With Fusarium solani ACCC 36223, 15.7 g/l genipin in the ethyl acetate phase was obtained, corresponding to space-time yields of 0.654 g l^-1 h^-1.

• Microbiology and Biotechnology Letters
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• v.24 no.4
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• pp.385-392
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• 1996

Bacillus stearotheymophilus, a potent xylanolytic bacterium isolated from soil, was tested for the strain's strategies of pentose utilization and the evidence of substrate preferences. The strain metabolized glucose, xylose, ribose, maltose, cellobiose, sucrose, arabinose and xylitol. The efficacy of the sugars as a carbon and energy source in this strain was of the order named above. The organism, however, could not grow on glycerol as a sole growth substrate. During cultivation on a mixture of glucose and xylose or arabinose, the major hydrolytic products of xylan, B. stearothermophilus displayed classical diauxic growth in which glucose was utilized during the first phase. On the other hand, the pentose utilization was prevented immediately upon addition of glucose. Cellobiose was preferred over xylose or arabinose. In contrast, maltose and pentose were co-utilized, and also no preference on between xylose and arabinose. Enzymatic studies indicated that B. stearothermophilus possessed constitutive hexokinase, a key enzyme of the glucose metabolic system. While, the production of $^{D}$-xylose isomerase, $^{D}$-xylulokinase and $^{D}$-arabinose isomerase essential for pentose phosphate pathway were induced by xylose, xylan, and xylitol but repressed by glucose. Taken together, the results suggested that the sequential utilization of B. stearothermophilus would be mediated by catabolite regulatory mechanisms such as catabolite inhibition or inducer exclusion.

• Magazine of the Korean Society of Agricultural Engineers
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• v.32 no.3
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• pp.102-115
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• 1990

Importance of anaerobic digestion as an energy generating device has been increased as fuel shortage becomes serieous. Several modification methods on the conventional digesters including Powdered Activated Carbon (PAC) addition and two-phase digestion were studied to enhance the gas production. This study investigated the effects of PAC on anaerobic digestion of chicken manure in terms of gas production and sludge stabilization. As a first experiment, an optimum PAC dose for efficient gas production was determined in a batch test. In semi-continuous experiments, an optimum Sludge Retention Time (SRT) at that PAC concentration and an overall substate utilization rate coefficient were investigated. A portion of gas increased by PAC addition was estimated using a substrate utilization rate coefficient of microorganisms attached on PAC. This test was performed in batch experiments using acetic acid as a substrate. The digesters for all experiments were kept 35${\pm}$ 1˚C in a heated water bath. Mixing was performed manually once a day and the produced gas was collected for daily reading. The following conclusions were made for this study. 1. Cptimum PAC concentration was 5% total solids, where gas production rate was increased by 20 percents. 2. Optimum SRT was 7.5 days. 3. Substrate utilization rate coefficient of microorganisms attached on PAC was about twice as much as that of suspended ones.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.10 no.9
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• pp.4023-4043
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• 2016

Network virtualization has been regarded as a core attribute of the Future Internet. In a network virtualization environment (NVE), multiple heterogeneous virtual networks can coexist on a shared substrate network. Thus, a substrate network failure may affect multiple virtual networks. In this case, it is increasingly critical to provide survivability for the virtual networks against the substrate network failures. Previous research focused on mechanisms that ensure the resilience of the virtual network. However, the resource efficiency is still important to make the mapping scheme practical. In this paper, we study the survivable virtual network embedding mechanisms against substrate link and node failure from the perspective of improving the resource efficiency. For substrate link survivability, we propose a load-balancing and re-configuration strategy to improve the acceptance ratio and bandwidth utilization ratio. For substrate node survivability, we develop a minimum cost heuristic based on a divided network model and a backup resource cost model, which can both satisfy the location constraints of virtual node and increase the sharing degree of the backup resources. Simulations are conducted to evaluate the performance of the solutions. The proposed load balancing and re-configuration strategy for substrate link survivability outperforms other approaches in terms of acceptance ratio and bandwidth utilization ratio. And the proposed minimum cost heuristic for substrate node survivability gets a good performance in term of acceptance ratio.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.10 no.2
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• pp.522-541
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• 2016

Network virtualization provides an effective way to overcome the Internet ossification problem. As one of the main challenges in network virtualization, virtual network embedding refers to mapping multiple virtual networks onto a shared substrate network. However, existing heuristic embedding algorithms evaluate the embedding potential of the nodes simply by the product of different resource attributes, which would result in an unbalanced embedding. Furthermore, ignoring the hops of substrate paths that the virtual links would be mapped onto may restrict the ability of the substrate network to accept additional virtual network requests, and lead to low utilization rate of resource. In this paper, we introduce and extend five node attributes that quantify the embedding potential of the nodes from both the local and global views, and adopt the technique for order preference by similarity ideal solution (TOPSIS) to rank the nodes, aiming at balancing different node attributes to increase the utilization rate of resource. Moreover, we propose a novel two-stage virtual network embedding algorithm, which maps the virtual nodes onto the substrate nodes according to the node ranks, and adopts a shortest path-based algorithm to map the virtual links. Simulation results show that the new algorithm significantly increases the long-term average revenue, the long-term revenue to cost ratio and the acceptance ratio.

• Korean Journal of Environmental Agriculture
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• v.38 no.4
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• pp.219-224
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• 2019

BACKGROUND: Soil bacteria play important roles in organic matter decomposition and nutrient cycling during the non-growing season. The purpose of this study was to investigate the effects of soil management and chemical properties on the utilization of carbon sources by soil bacteria in paddy fields. METHODS AND RESULTS: The Biolog EcoPlate was used for analyzing community-level carbon substrate utilization profiles of soil bacteria. Soils were collected from the following three types of areas: plain, interface and mountain areas, which were tested to investigate the topology effect. The results of canonical correspondence analysis and Kendall rank correlation analysis showed that soil C/N ratio and NH₄⁺ influenced utilization of carbon sources by bacteria. The utilization of carbohydrates and complex carbon sources were positively correlated with NH₄⁺ concentration. Cultivated paddy fields were compared with adjacent abandoned fields to investigate the impact of cultivation cessation. The level of utilization of putrescine was lower in abandoned fields than in cultivated fields. Monoculture fields were compared with double cropping fields cultivated with barley to investigate the impact of winter crop cultivation. Cropping system altered bacterial use of carbon sources, as reflected by the enhanced utilization of 2-hydroxy benzoic acid under monoculture conditions. CONCLUSION: These results show that soil use intensity and topological characteristics have a minimal impact on soil bacterial functioning in relation to carbon substrate utilization. Moreover, soil chemical properties were found to be important factors determining the physiological profile of the soil bacterial community in paddy fields.

• Journal of Microbiology and Biotechnology
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• v.18 no.5
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• pp.852-858
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• 2008

An extracellular protease (Mc1) was isolated from the nematode-trapping fungus Monacrosporium cystosporium by gel filtration, anion-exchange, and hydrophobic interaction chromatographies. This protease had a molecular mass of approximately 38 kDa and displayed an optimal activity at pH 7-9 and $56^{\circ}C$ (over 30 min). Its proteolytic activity was highly sensitive to the serine protease inhibitor PMSF (phenylmethylsulfonylfluoride, 0.1 mM), indicating that it belonged to the serine-type peptidase group. The Michaelis constant ($K_m$) and $V_max$ for substrate N-Suc-Ala-Ala-Pro-Phe-pNA were $1.67{\times}10^{-4}\;M$ and 0.6071 $OD_{410}$ per 30 s, respectively. This protease could degrade a broad range of substrates including casein, gelatin, BSA (bovine serum albumin), and nematode cuticle. Moreover, the enzyme could immobilize the free-living nematode Panagrellus redivivus and the pine wood nematode Bursaphelenchus xylophilus, suggesting that it might playa role in infection against nematodes. The encoding gene of Mc1 was composed of one intron and two exons, coding for a polypeptide of 405 amino acid residues. The deduced amino acid sequence of Mcl showed 61.4-91.9% identity to serine proteases from other nematode-trapping fungi. Our results identified that Mcl possessed biochemical properties including optimal reaction condition and substrate preference that are different from previously identified serine proteases.