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In Vitro Development of Reconstructed Bovine Embryos and Fate of Donor Mitochondria Following Nuclear Injection of Cumulus Cells

  • Do, Jeong-Tae;Lee, Bo-Yon;Kim, Seung-Bo;Lee, Hoon-Taek;Chung, Kil-Saeng
    • Proceedings of the KSAR Conference
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    • 2001.03a
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    • pp.11-11
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    • 2001
  • In this study, we examined the developmental potential of reconstructed bovine embryos and the fate of donor mitochondria during their preimplantation development after nuclear transfer. Isolated cumulus cells were used as donor cells in nuclear transfer. Cumulus cells labelled with MitoTracker Green FM fluorochrome were injected into enucleated bovine MII oocytes and cultured in vitro. MitoTracker labelling on donor cells did not have a detrimental effect on blastocyst formation following nuclear transfer. Cleavage rate was about 69%(56/81) and blastocyst formation rate was 6.2% (5/81) at 7 days after nuclear transfer. The labelled mitochondria dispersed to the cytoplasm and became distributed among blastomeres and could be identified up to the 8- to 15-cell stages. Small patches of mitochondria were detected in some 8- to 15-cell stage embryos (5/20). However, donor mitochondria were not detected in embryos at the 16-cell stage and subsequent developmental stages. In the control group, mitochondria could be identified in arrested 1-cell embryos up to 7 days after nuclear transfer These results suggest that donor mitochondria disappear from recipient cytoplasm before 16-cell stage following nuclear transfer in reconstructed bovine embryos.

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Industrial dairy wastewater purification by shear-enhanced membrane filtration: The effects of vibration

  • Kertesz, Szabolcs
    • Membrane and Water Treatment
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    • v.5 no.2
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    • pp.73-86
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    • 2014
  • Membrane fouling is a major challenge limiting the use of membrane applications. In this study high induced shear rates were utilized at the membrane surface in order to reduce the organic and inorganic scaling by using the torsional vibration of flat sheet membranes. The performances of a vibratory shear-enhanced processing (VSEP) system for the ultrafiltration (UF), nanofiltration (NF) and reverse osmosis (RO) membrane filtration of industrial dairy wastewater were investigated. The vibration and non-vibration methods were compared with the same membrane and operational parameters during the purification of real dairy industrial process wastewater. In the initial experiments, short-term tests were carried out in which the effects of vibration amplitude, recirculation flow rate and transmembrane pressure were measured and compared. The permeate flux, turbidity, conductivity and chemical oxygen demand (COD) reduction of dairy wastewater were investigated by using UF, NF and RO membranes with vibration and non-vibration methods. In the subsequent experiments, concentration tests were also carried out. Finally, scanning electron microscopy (SEM) revealed that the vibration method gave a better performance, which can be attributed to the higher membrane shear rate, which reduces the concentration of solids at the membrane, and the transmission.

Improvement of the Advanced Treatment for Nitrogen Removal of Acrylic Fiber Wastewater (아크릴섬유 폐수의 생물학적 질소제거공정의 개선)

  • Lee, Chan-Won;Cho, In-Sung;Lim, Kyeong-Won
    • Journal of Environmental Science International
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    • v.15 no.5
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    • pp.439-446
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    • 2006
  • The effluent discharge standards of industrial wastewater has become more stringent since 2003. Many industrial wastewater treatment plants has been upgraded to advanced treatment facilities. There are high concentrations of nitrate(>200 mg/L) and ammonium(>50 mg/L) nitrogen in the acrylic fiber wastewater of H textile Co. Wastewater from acrylic fiber industry containing acrylonitrile, which may affect the subsequent biological treatment process. Manufacturing of acrylic fiber also produces shock loadings. Excessive acrylonitrile and polymer debris produced in the polymerization process was screened, coagulated with CaO and settled down. A preaeration system was added to treat this high pH effluent to remove volatile organic compound and ammonia nitrogen by the air stripping effect. it was found that nitrification rate was not sufficient in the Anoxic/Oxic(AO) process. One denitrification tank was converted to nitrification reactor to extend HRT of nitrification. Nitrification rate of ammonia nitrogen was promoted from 32% to 67% by this modification and effluent nitrogen concentration was well satisfied with the effluent standards since then.

Effect of the density profile of a star on the bolometric light curve in tidal disruption events

  • Park, Gwanwoo;Kimitake, Hayasaki
    • The Bulletin of The Korean Astronomical Society
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    • v.43 no.1
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    • pp.56.1-56.1
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    • 2018
  • Tidal disruption events (TDEs) provide evidence for quiescent supermassive black holes (SMBHs) in the centers of inactive galaxies. TDEs occur when a star on a parabolic orbit approaches close enough to a SMBH to be disrupted by the tidal force of the SMBH. The subsequent super-Eddington accretion of stellar debris falling back to the SMBH produces a characteristic flare lasting several months. The theoretically expected bolometric light curve decays with time as proportional to $t^{-5/3}$. However, the light curves observed in most of the optical-UV TDEs deviate from the $t^{-5/3}$ decay rate especially at early time, while the light curves of some soft-X-ray TDEs are overall in good agreement with the $t^{-5/3}$ law. Therefore, it is required to construct the theoretical model for explaining these light curve variations consistently. In this paper, we revisit the mass fallback rates analytically and semi-analytically by taking account of the structure of the star, which is simply modeled by the polytrope. We find the relation between a polytropic index and the power law index of the mass fallback rate. We also discuss whether and how the decay curves, which we derived, fit the observed ones.

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Analysis for Evaluating the Impact of PEVs on New-Town Distribution System in Korea

  • Choi, Sang-Bong
    • Journal of Electrical Engineering and Technology
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    • v.10 no.3
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    • pp.859-864
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    • 2015
  • This paper analyzes the impact of Plug-in Electric vehicles(PEVs) on power demand and voltage change when PEVs are connected to the domestic distribution system. Specifically, it assesses PEVs charging load by charging method in accordance with PEVs penetration scenarios, its percentage of total load, and voltage range under load conditions. Concretely, we develop EMTDC modelling to perform a voltage distribution analysis when the PEVs charging system by their charging scenario was connected to the distribution system under the load condition. Furthermore we present evaluation algorithm to determine whether it is possible to adjust it such that it is in the allowed range by applying ULTC when the voltage change rate by PEVs charging scenario exceed its allowed range. Also, detailed analysis of the impact of PEVs on power distribution system was carried out by calculating existing electric power load and additional PEVs charge load by each scenario on new-town in Korea to estimate total load increases, and also by interpreting the subsequent voltage range for system circuits and demonstrating conditions for countermeasures. It was concluded that total loads including PEVs charging load on new-town distribution system in Korea by PEVs penetration scenario increase significantly, and the voltage range when considering ULTC, is allowable in terms of voltage tolerance range up to a PEVs penetration of 20% by scenario. Finally, we propose the charging capacity of PEVs that can delay the reinforcement of power distribution system while satisfying the permitted voltage change rate conditions when PEVs charging load is connected to the power distribution system by their charging penetration scenario.

Optimization of β-Cyclodextrin Recycling Process for Cholesterol Removal in Cream

  • Kwak, H.S.;Suh, H.M.;Ahn, J.;Kwon, H.J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.4
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    • pp.548-552
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    • 2001
  • This study was designed to find optimum conditions of four different factors (ratio of solvent to cholesterol-$\beta$-cyclodextrin complex, mixing speed, mixing temperature, and mixing time) for cholesterol dissociation in cream. Using the ratio of 6 to 1 (solvent to the complex) showed the highest cholesterol dissociation rate (82.50%) when mixed at 100 rpm at $50^{\circ}C$ for 1 h. Mixing speed did not significantly affect the cholesterol dissociation. Also, mixing time appeared to be insignificant. The optimum mixing temperature was $50^{\circ}C$ and mixing at $40^{\circ}C$ resulted in a significantly lower rate, compared with that at $50^{\circ}C$. In a subsequent experiment, using recycled $\beta$-cyclodextrin only showed 75.07% of cholesterol removal in cream, while the mixture of recycled to unused $\beta$-CD with the ratio of 6 to 4 increased cholesterol removal to 95.59%, which is highly close to that of 100% unused $\beta$-CD.

Conceptual Study of Brain Dedicated PET Improving Sensitivity

  • Shin, Han-Back;Choi, Yong;Huh, Yoonsuk;Jung, Jin Ho;Suh, Tae Suk
    • Progress in Medical Physics
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    • v.27 no.4
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    • pp.236-240
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    • 2016
  • The purpose of this study is to propose a novel high sensitivity neuro-PET design. The improvement of sensitivity in neuro-PET is important because it can reduce scan time and/or radiation dose. In this study, we proposed a novel PET detector design that combined conical shape detector with cylindrical one to obtain high sensitivity. The sensitivity as a function of the oblique angle and the ratio of the conical to cylindrical portion was estimated to optimize the design of brain PET using Monte Carlo simulation tool, GATE. An axial sensitivity and misplacement rate by penetration of ${\gamma}$ rays were also estimated to evaluate the performance of the proposed PET. The sensitivity was improved by 36% at the center of axial FOV. This value was similar to the calculated value. The misplacement rate of conical shaped PET was about 5% higher than the conventional PET. The results of this study demonstrated the conical detector proposed in this study could provide subsequent improvement in sensitivity which could allow to design high sensitivity PET for brain imaging.

Chemical Mechanical Polishing Characteristics of BTO Thin Film for Vertical Sidewall Patterning of High-Density Memory Capacitor (고집적 메모리 커패시터의 Vertical Sidewall Patterning을 위한 BTO 박막의 CMP 특성)

  • Ko, Pil-Ju;Park, Sung-Woo;Lee, Kang-Yeon;Lee, Woo-Sun;Seo, Yong-Jin
    • The Transactions of the Korean Institute of Electrical Engineers C
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    • v.55 no.3
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    • pp.116-121
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    • 2006
  • Most high-k materials are well known not to be etched easily, Some problems such as low etch rate poor sidewall angle, plasma damage, and process complexity were emerged from the high-density DRAM fabrication. Chemical mechanical polishing (CMP) by a damascene process was proposed to pattern this high-k material was polished with some commercial silica slurry as a function of pH variation. Sufficient removal rate with adequate selectivity to realize the pattern mask of tera-ethyl ortho-silicate (TEOS) film for the vertical sidewall angle were obtained. The changes of X-ray diffraction pattern and dielectric constant by CMP process were negligible. The planarization was also achieved for the subsequent multi-level processes. Our new CMP approach will provide a guideline for effective patterning of high-k material by CMP technique.

The Influence of Weaning Time on Deer Performance

  • Bao, Y.M.;Ru, Y.J.;Glatz, P.C.;Miao, Z.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.4
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    • pp.569-582
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    • 2004
  • Current growth in the deer industry is 20% per annum as the demand increases for venison in Europe, including the increased use in Asia of antlers as a natural medicine by humans. The deer industry in Australia has developed rapidly, but farm profitability has fluctuated markedly. The knowledge on deer farming has largely been adopted from New Zealand (NZ) and the United Kingdom (UK) although the environmental conditions in these deer growing countries differ markedly from Australia. The practice used for weaning is one of the key factors influencing the profitability of the industry. However, a wide range in weaning dates are practised by farmers leading to the question of whether deer should be weaned after mating to reduce the stress during weaning or should they be weaned in the pre-rut period to allow does to recover their body condition for the next reproduction cycle. This review examines the effect of weaning time on the performance of does and hinds and the subsequent growth rate of the fawns and calves. Gaps in knowledge of weaning procedures and nutritional management for early weaned deer are identified.

Effects of Natural Organic Matter (NOM) on Cr(Ⅵ) reduction by Fe(II) (Fe(II)을 이용한 Cr(Ⅵ) 환원시 천연유기물의 영향)

  • 한인섭
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 1999.10a
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    • pp.81-84
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    • 1999
  • The aqueous geochemical characteristics of Cr(III) and Cr(Ⅵ) in environmental systems are very different from one another: Cr(Ⅵ) is highly soluble, mobile and toxic relative to Cr(III) Reduction of Cr(Ⅵ) to Cr(III) are beneficial in aquatic systems because of the transformation of a highly mobile and toxic species to one having a low solubility in water, thus simultaneously decreasing chromium mobility and toxicity. Fe(II) species are excellent reductants for transforming Cr(Ⅵ) to Cr(III), and in addition, keeping Cr(III) concentrations below the drinking water standard of 52 ppb at pH values between 5 and 11. Investigations of the effects of NOM on Cr(Ⅵ) reduction are for examining the feasibility of using ferrous iron to reduce hexavalent chromium in subsurface environments. Experiments in the presence of soils, however, showed that the solid phase consumes some of the reducing capacity of Fe(II) and makes the overall reduction kinetics slower. The soil components bring about consumption of the ferrous iron reductant. Particular attention is devoted to the complexation of Fe(II) by NOM and the subsequent effect on Cr(Ⅵ) reduction. Cr(Ⅵ) reduction rate by Fe(II) was affected by the presence of NOM (humic acid), The effects of humic acid was different from the solution pH values and the concentration of humic acid. It was probably due to the reactions between humic acid and Cr(Ⅵ), humic acid and Fe(II), and between Cr(Ⅵ) and Fe(II), at each pH.

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