• Advances in Traditional Medicine
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• 제7권4호
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• pp.395-408
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• 2007

This study reports the analgesic, anti-inflammatory and membrane-stabilizing property of alcoholic extract of Ophiorrhiza nicobarica (ON), a wild herb, used as an anti-infective ethnomedicine of Nicobarese and Shompen tribes of Great Nicobar Island, India. We for the first time investigated the analgesic and antiinflammatory potential of this herb in acute, subacute and chronic model of inflammation in Swiss albino mice and Wistar albino rats, along with sheep RBC-induced sensitivity and membrane stabilization. The acetic acid induced writhing, tail flick and tail immersion tests are used as a model for evaluating analgesic activity; while the carrageenin-induced paw oedema was used as the model for acute inflammation, dextran-induced oedema as sub-acute and cotton-pellateinduced granuloma as chronic inflammatory model. The probable mode by which ON mediate its effect on inflammatory conditions was studied on sheep RBC-induced sensitivity and membrane stabilization. The in vitro results revealed that the ON extract possesses significant (P < 0.05) dose dependent analgesic and antiinflammatory activity at 200 and 300 mg/kg and its fractions at 50 mg/kg, p.o. respectively, compared to the control groups. However, the extract failed to exhibit membrane-stabilizing property as it unable to reduce the level of haemolysis of RBC exposed to hypotonic solution. The acute toxicity studies of ON extract in rats and mice revealed that the extract was nontoxic even up to 3.0 g/kg body weight of the animals, with a high safety profile. We have isolated ursolic acid, ${\beta}$-sitosterol and harmaline respectively, from the bioactive part of the extract. The results indicated that the O. nicobarica is indeed beneficial in primary health care, and suggest that its anti-inflammatory activity may not be related to membrane-stabilization.

• 대한한의학방제학회지
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• 제17권2호
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• pp.123-132
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• 2009

Acetaminophen (AP) is widely used as an over-the-counter analgesic and antipyretic drug. AP-induced hepatotoxicity is a common consequence of AP overdose and may lead to acute liver failure. In this study, we investigated the liver damage in mice using single dose (300 mg/kg) of AP and the possible protective effects of administration (50-200 mg/kg body weight) of Joo-Juk on acetaminophen-induced liver damage in mice. The alanine aminotransferase (ALT), and aspartate aminotransferase (AST) activities were determined in the plasma of mice. The effect of Joo-Juk on lipid peroxidation product thiobarbituric reacting substances (TBARS) and some antioxidant enzymes superoxide dismutase (SOD), catalase, d-aminolevulinate dehydratase ($\sigma$-ALA-D) activities, and gluthathione peroxidase (GPx), were also evaluated in the mouse liver homogenate. AP caused liver damage as evident by statistically significant increased in plasma activities of AST and ALT. There were statistically significant losses in the activities of SOD, catalase, $\sigma$-ALA-D, and GPx and an increase in TBARS in the liver of AP-treated group compared with the control group. However, Joo-Juk was able to counteract these effects. These results suggest that Joo-juk can act as hepato-protectant against AP toxicity and is a good candidate for further evaluation as an effective chemotherapeutic agent.

• Natural Product Sciences
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• 제16권3호
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• pp.169-174
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• 2010

The seed of Phaseolus calcaratus Roxburgh (PHCR) is traditionally used for anti-pyretic and antiinflammatory effects. Although these effects are believed to be related to its antioxidant potential, little information is available for the mechanisms by which PHCR seed might scavenge free radicals or otherwise act as an antioxidant. In the present study, we purified some fractions from the ethanol extract of PHCR seed and evaluated each fraction's ability to scavenge free radicals generated by cell-free systems. We also identified active compound that is putatively responsible for free radical scavenging by analyzing NMR spectra. PHCR samples exhibited a concentration-dependent radical scavenging activity against hydroxyl radicals, superoxide anions, and DPPH radicals. Of the samples tested, a methanol-eluted sub-fraction from the PHCR extract, named $FF_4$, scavenged these radicals more effectively than the other fractions. We identified catechin-7-O-$\beta$-Dglucopyranoside as the active compound responsible for free radical scavenging potential of $FF_4$.

• Molecules and Cells
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• 제38권5호
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• pp.416-425
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• 2015

NF-E2-related factor 2 (Nrf2), a basic leucine zipper transcription factor, has recently received a great deal of attention as an important molecule that enhances antioxidative defenses and induces resistance to chemotherapy or radiotherapy. In this study, we investigated the apoptosis-inducing and Nrf2- upregulating effects of quercetin on malignant mesothelioma (MM) MSTO-211H and H2452 cells. Quercetin treatment inhibited cell growth and led to upregulation of Nrf2 at both the mRNA and protein levels without altering the ubiquitination and extending the half-life of the Nrf2 protein. Following treatment with quercetin, analyses of the nuclear level of Nrf2, Nrf2 antioxidant response element-binding assay, Nrf2 promoter-luc assay, and RT-PCR toward the Nrf2-regulated gene, heme oxygenase-1, demonstrated that the induced Nrf2 is transcriptionally active. Knockdown of Nrf2 expression with siRNA enhanced cytotoxicity due to the induction of apoptosis, as evidenced by an increase in the level of proapoptotic Bax, a decrease in the level of antiapoptotic Bcl-2 with enhanced cleavage of caspase-3 and PARP proteins, the appearance of a sub-$G_0/G_1$ peak in the flow cytometric assay, and increased percentage of apoptotic propensities in the annexin V binding assay. Effective reversal of apoptosis was observed following pretreatment with the pan-caspase inhibitor Z-VAD. Moreover, Nrf2 knockdown exhibited increased sensitivity to the anticancer drug, cisplatin, presumably by potentiating the oxidative stress induced by cisplatin. Collectively, our data demonstrate the importance of Nrf2 in cytoprotection, survival, and drug resistance with implications for the potential significance of targeting Nrf2 as a promising strategy for overcoming resistance to chemotherapeutics in MM.

• 한국방재안전학회논문집
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• 제13권3호
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• pp.59-74
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• 2020

본 연구는 참여적 연구방법인 포토보이스 방법을 적용하여 대학생들의 인터넷 중독에 대한 주관적 인식에 대해 심층적으로 탐색해 보고자 하였다. 연구대상은 G대학의 대학생 9명이며, 일반적인 포토보이스 연구절차에 따라 오리엔테이션, 연구 참여자들의 사진촬영을 통한 자료수집, 사진 선정, 맥락화, 범주화 과정으로 자료를 분석하였다. 연구결과 인터넷 중독의 의미, 인터넷 중독이 삶에 미치는 영향, 인터넷 중독에서 벗어나는 방안이라는 주제에 따라 15개의 하위범주가 도출 되었다. 인터넷 중독의 의미에 대한 인식은 '자기조절 능력상실', '출구 없는 습관', '세상과의 단절', '나를 잃어버림', '자발적 감옥'이라는 5가지 범주로 나타났다. 인터넷 중독이 삶에 미치는 영향은 5가지 범주로 '건강 악화, '사회적 고립, '망가진 일상', '익명으로 인한 폭력성', '나의 발견과 삶의 확장'이었다. 인터넷 중독에서 벗어나는 방법에 대해서는 '자기바라보기', '새로운 취미생활', '주변의 도움', '현실속의 만남', '강제적 차단'이 도출되었다. 대학생의 시선으로 바라본 인터넷 중독에 대한 논의와 올바른 인터넷 사용을 조력할 수 있는 상담 프로그램 구성에 대한 시사점을 제언하였다.

• 전자공학회논문지SC
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• 제49권1호
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• pp.39-46
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• 2012

IT 및 통신 기술의 발달로 의료시스템 분양에 적용하여 원격진료 서비스를 제공하게 되었다. 원격진료 서비스는 인력 및 장비가 부족한 보건소, 학교, 도서지역 등에서 원격진료 서비스를 이용하여 2, 3차 의료기관의 의료진과 원격 자문과 진단 서비스를 통하여 양질의 서비스를 받을 수 있다. 원격진료를 이용하는 환자와 의료진은 다양한 통신망을 사용하여 원격진료 서비스를 이용 할 수 있지만, 통신망에 따라 대역폭의 차이와 공인 IP, 사설 IP의 특성으로 인하여 원격진료 서비스를 원활히 이용하는데 어려움이 있다. 따라서 본 논문에서는 다양한 네트워크 환경에서도 원격진료 서비스를 원활하게 이용 할 수 있고, 의료진과 환자 사이의 연결 리스트를 관리하여 효율적으로 원격진료 서비스를 제공할 수 있도록 다중접속 제어서버를 구현하였다. 다중접속 제어서버는 환자와 의료진의 리스트관리, 네트워크 타입, 사용자 장치타입을 이용하여 최적의 정책을 결정하여('Flowing'과 'Bypassing') 서비스하고, 사용자(의료진과 환자)의 연결 정보를 모니터링 할 수 있는 시스템을 구현하였다.

• 한국응급구조학회지
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• 제12권3호
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• pp.71-86
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• 2008

Purpose : This study was to examine the influence of job stress of 119 Emergency Medical Technician on fatigue and job satisfaction. Methods : Subjects of this study were 228 Emergency Medical Technicians who worked at 119 Safety Center of total 92 fire stations in 12 cities of Gyeonggido including U, N, H, B, A, S, Y, G, G, G, P and I and the period of data collection was from July 11 to Sep. 10, 2007. Collected data were analyzed with SPSS 14.0 version. Results : Conclusions of this study were as follows. 1) Factor related to organization among job stress factors had the greatest influence on fatigue (B = 0.334, p < 0.01), followed by crisis situation factor (B = 0.2042, p < 0.01), inappropriate treatment factor (B = 0.174, p < 0.05), role conflict factor as special job (B = 0.109, p < 0.05) and professional knowledge and technique factor (B = 0.109, p < 0.05), and the influence of job stress factors on fatigue was explained as $R^2=0.340$. 2) Mental burden factor of job stress factors had the greatest influence on job satisfaction (B = -0.606, p < 0.01), followed by organization factor (B = -0.473, p < 0.01) and interpersonal relation and conflict factor (B = -0.339, p < 0.01), and the influence of job stress factor on fatigue was explained as $R^2=0.308$. Conclusions : Job stress of 119 rescuer is increasing continuously and such an accumulated stress lowers the job efficiency. In order to reduce, job stress, it is important to extend(${\rightarrow}$ have) proper self-development, maintain close relationships and mutual correlations among members of organization in personal dimension and to solve the role conflicts, define the organizational roles and simplify excessive job description into concrete work.

• Nuclear Engineering and Technology
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• 제52권1호
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• pp.155-163
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• 2020

To investigate the optimal detector material for prompt gamma imaging during boron neutron capture therapy, in this study, we evaluated the characteristic regarding radiation reaction of available detector materials using a Monte Carlo simulation. Sixteen detector materials used for radiation detection were investigated to assess their advantages and drawbacks. The estimations used previous experimental data to build the simulation codes. The energy resolution and detection efficiency of each material was investigated, and prompt gamma images during BNCT simulation were acquired using only the detectors that showed good performance in our preliminary data. From the simulation, we could evaluate the majority of detector materials in BNCT and also could acquire a prompt gamma image using the six high ranked-detector materials and lutetium yttrium oxyorthosilicate. We provide a strategy to select an optimal detector material for the prompt gamma imaging during BNCT with three conclusions.

• Biomolecules & Therapeutics
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• 제20권3호
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• pp.273-279
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• 2012

Hesperidin, a flavanone present in citrus fruits, has been studied as potential therapeutic agents that have anti-tumor activity and apoptotic effects in several cancers, but there is no report about the apoptotic effect of hesperidin in human malignant pleural mesothelioma through the specificity protein 1 (Sp1) protein. We investigated whether hesperidin inhibited cell growth and regulated Sp1 target proteins by suppressing the levels of Sp1 protein in MSTO-211H cells. The $IC_{50}$ value of hesperidin was determined to be 152.3 ${\mu}M$ in MSTO-211H cells for 48 h. Our results suggested that hesperidin (0-160 ${\mu}M$) decreased cell viability, and induced apoptotic cell death. Hesperidin increased Sub-$G_1$ population in MSTO-211H cells. Hesperidin significantly suppressed mRNA/protein level of Sp1 and modulated the expression level of the Sp1 regulatory protein such as p27, p21, cyclin D1, Mcl-1, and survivin in mesothelioma cells. Also, hesperidin induced apoptotic signaling including: cleavages of Bid, caspase-3, and PARP, upregulation of Bax, and down-regulation of Bcl-$_{xl}$ in mesothelioma cells. These results show that hesperidin suppressed mesothelioma cell growth through inhibition of Sp1. In this study, we demonstrated that Sp1 acts as a novel molecular target of hesperidin in human malignant pleural mesothelioma.

• Journal of Korean Neurosurgical Society
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• 제64권1호
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• pp.60-68
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• 2021

Objective : Until recently, the transfemoral approach (TFA) was used as the primary method of arterial approach in acute ischemic stroke (AIS). However, TFA resulted in longer reperfusion times and worse outcomes in the mechanical thrombectomy (MT) of patients with complex aortic arches and significant carotid tortuosity. We found that the transradial approach (TRA) is a more favorable alternative approach for MT in such cases. Methods : We performed a retrospective review of our institutional database to identify 202 patients who underwent MT for AIS between February 2015 and December 2019. Patient characteristics, cause of TFA failure, procedure time, intra-procedural complications, and outcomes were recorded. Results : Eleven (5.4%) of 202 patients, who underwent MT for AIS, crossed over to TRA for recanalization, and eight (72%) of 11 achieved successful recanalization (≥modified Treatment in Cerebral Infarction 2b). The mean age (mean±standard deviation [median]) was 82.3±6.6 (76) years, and five of the 11 patients were male. The last seen normal to puncture time was 467.9±264.72 (264) minutes; baseline National Institutes of Health Stroke Scale score was 28.9±14.5 (16). Six (55%) of the 11 patients had right vertebrobasilar occlusions, and the remaining five (45%) had anterior circulation occlusive disease. The time from groin puncture to final recanalization time (overall procedural time) was 78.0±20.1 (62) minutes. The mean crossover time from TFA to TRA was 45.2±10.5 (41) minutes. The mean time from radial puncture to final recanalization was 33.8±10.5 (28) minutes. Distal thrombus migration events in previously unaffected territories occurred in 3/8 patients (37%). At 90 days, three patients (28%) had a favorable clinical outcome. Conclusion : Although rare, failure of TFA has been known to occur during MT for AIS. Our results demonstrate that TRA may be an alternative option for AIS intervention for select patients with subsequent timely revascularization. However, the incidence of distal thrombus migration was high, and the first puncture to reperfusion time was prolonged because of the time taken for the crossover to TRA after failure of TFA. This study provides some evidence that the TRA may be a viable alternative option to the TFA for MT of AIS.