• BMB Reports
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• v.40 no.5
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• pp.697-707
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• 2007

Cytochrome $cbb_3$ oxidase is a member of the heme-copper oxidase superfamily that catalyses the reduction of molecular oxygen to the water and conserves the liberated energy in the form of a proton gradient. Comparison of the amino acid sequences of subunit I from different classes of heme-copper oxidases showed that transmembrane helix VIII and the loop between transmembrane helices IX and X contain five highly conserved polar residues; Ser333, Ser340, Thr350, Asn390 and Thr394. To determine the relationship between these conserved amino acids and the activity and assembly of the $cbb_3$ oxidase in Rhodobacter capsulatus, each of these five conserved amino acids was substituted for alanine by site-directed mutagenesis. The effects of these mutations on catalytic activity were determined using a NADI plate assay and by measurements of the rate of oxygen consumption. The consequence of these mutations for the structural integrity of the $cbb_3$ oxidase was determined by SDS-PAGE analysis of chromatophore membranes followed by TMBZ staining. The results indicate that the Asn390Ala mutation led to a complete loss of enzyme activity and that the Ser333Ala mutation decreased the activity significantly. The remaining mutants cause a partial loss of catalytic activity. All of the mutant enzymes, except Asn390Ala, were apparently correctly assembled and stable in the membrane of the R. capsulatus.

• Journal of the Korea Society of Computer and Information
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• v.25 no.10
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• pp.193-201
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• 2020

This study is to evaluate the effectiveness of problem-solving ability by applying a team-based learning model to the classes of humanities and social science students, and to conduct a structural model analysis on the relationship between sub-factors. Team-based learning was conducted six times in six teams with 30 students in the second and third grades of the humanities and social sciences. The problem solving ability score of the target students was significantly higher after team-based learning and was statistically significant. There was no problem in normality with the latent variables, which are the sub-factors of problem solving ability, and the factor load value was statistically significant at the .001 level in the confirmatory factor analysis of the observed variables for the latent variables, which was a valid model. A good level of fitness was also shown in the verification of the fitness of the research model. As a result, it was analyzed that latent variables of cause analysis, problem clarification, planning execution, performance evaluation, and alternative development had an indirect or direct influence on each other.

• Korean Journal of Community Nutrition
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• v.22 no.3
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• pp.218-227
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• 2017

Objectives: The purpose of the study was to develop dietary change program items that could be used to improve dietary life of the elderly and investigate their validity. Methods: The survey was were analyzed by SPSS program (Ver. 21) and descriptive statistics was performed; a t-test, ${\chi}^2$ test, One-way ANOVA and Friedman test were used to determine the priority. Results: Programs for feeding senior citizens that need to be newly established are largely divided into two fields, namely, application of welfare facilities and application of home care, classified into large, medium and sub-classes. The large class was divided into nutrition management, sanitary control, and other health management. The medium class of nutrition management was divided into nutrition education, nutrition intervention, and menu management and supply. The sub-class was composed of division into application of welfare facilities for the elderly and application of home care for the same age group. Responses showed high rate saying that all the categorized items were necessary and valid. With respect to expectation effect on a community program for old people feeding, 'yes' was 65 people (55.6%) showing very high expectation toward the question whether a community program for old people feeding are newly set up. Conclusions: It is believed that nutrition for the aged will be improved and it will be a help not only to a small facilities without obligation of employing a dietician but also to the aged at home if a community program for old people feeding are newly established.

• Korean Journal of Human Ecology
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• v.23 no.5
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• pp.807-823
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• 2014

The purpose of this study is to verify the effects of storytelling-centered arts educational activities based on the famous painting appreciation on young children's appreciation ability of pictures and creativity. The study was conducted with a total of 50 5-year-old young children in two classes of B Day Care Center in the city of J, Jeollabukdo. The children were randomly divided into an experimental group(25) and a control group(25). The storytelling-centered arts educational activities based on the famous painting appreciation was conducted for the experimental group for 12 weeks from April 29, 2013 to July 19, 2013. The activities were composed of 3 steps of 'appreciation-expression-exhibition & assessment' on the basis of 2-week basic units. The appreciation activities were composed basing upon the types of the famous painting appreciation and storytelling, and expression and exhibition & assessment activities were composed of 4 areas of arts(music/movement/language/art). The results of the study are as follows. First, the storytelling-centered arts educational activities based on the famous painting appreciation generally improved young children's painting appreciation abilities in all sub-areas(observation and description/artist's intention of subject expression/mood of a work/material and technique/artistic factors/attitude to the work). Second, the storytelling-centered arts educational activities based on the famous painting appreciation generally improved young children's creativities in all sub-areas(originality/fluency/flexibility/imagination).

• Journal of the Korean Society of Clothing and Textiles
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• v.16 no.1 s.41
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• pp.85-96
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• 1992

The puropse of this study is to find out that whether the juvenile delinquency could be decreased by the revival of school uniform for the high school students. For this purpose, the difference and frequency of juvenile delinquency between uniformed students and none·uniformed students were surveyed. In addition, the relation among the students, social classes, receiving attitude for the sub-culture, with juvenile delinquency were also surveyed. For this study, 728 high school students were' studied with a questionaire. They were 328 ' uniformed students from 6 schools and 400 none-uniformed students from other 6 schools. The conclusions of this study are as follow: 1. The order of juvenile delinquency is resistance to their parents and teachers, cunning in examination, drinking, watching shameful video tapes, possession of dirty books and pictures, gambling, entering to thea rooms, billiard rooms, the prohibited cinema houses aild drinking houses, and smoking. The students' delinquency was chiefly misdeed against the public order and social position and most of them were without any victims. And there were no misdeed such as drug addiction, robbrry and bodily harm. 2. The difference of juvenile delinquency between uniformed and non·uniformed schools was not found. 3. More nisdeed were occurred when they were without uniform; especially in cases of entering to shameful video shops, drinking houses, tea rooms and billiard rooms. 4.The logical propriety that the juvenile delinquency would be decreased by putting on uniform to all students is scarce, because Ell misdeed were occurred when they were without uniform after school. Uniform were worn only when they go to school. 5. A negative effect between student's affection toward their parents and schools, and juvenile deliquency was found. Much they love their parents and schools, less misdeed occurred. 6. Positive effect between sub·culture and juvenile delinquency was found. The friendship with delinquent students gives a great influence to juvenile delinquency.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.6 no.11
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• pp.2784-2799
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• 2012

Biological data have been increased exponentially in recent years, and analyzing these data using data mining tools has become one of the major issues in the bioinformatics research community. This paper focuses on the protein construction process in higher organisms where the deoxyribonucleic acid, or DNA, sequence is filtered. In the process, "unmeaningful" DNA sub-sequences (called introns) are removed, and their meaningful counterparts (called exons) are retained. Accurate recognition of the boundaries between these two classes of sub-sequences, however, is known to be a difficult problem. Conventional approaches for recognizing these boundaries have sought for solely enhancing machine learning techniques, while inherent nature of the data themselves has been overlooked. In this paper we present an approach which makes use of the data attributes inherent to species in order to increase the accuracy of the boundary recognition. For experimentation, we have taken the data sets for four different species from the University of California Santa Cruz (UCSC) data repository, divided the data sets based on the species types, then trained a preprocessed version of the data sets on neural network(NN)-based and support vector machine(SVM)-based classifiers. As a result, we have observed that each species has its own specific features related to the splice sites, and that it implies there are related distances among species. To conclude, dividing the training data set based on species would increase the accuracy of predicting splicing junction and propose new insight to the biological research.

• Journal of Korean Home Economics Education Association
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• v.21 no.2
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• pp.203-215
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• 2009

A research problem for this study is formulated: "Is practical reasoning instruction effective on raising problem solving ability?" This study is a quasi-experimental study with independent variable of practical reasoning instruction and dependent variable of problem solving ability. Six class hours of experimental input for the 'Housing space' is implemented for an experimental group. T-test results show that practical reasoning instruction is effective on total problem solving ability whereas is not effective on 'implementing alternative action' sub-area of problem solving. This study suggests for the future studies to systematically design practical reasoning classes in consider of appropriate class times and sub-areas of problem solving. Input of an experienced teacher of practical reasoning is also recommended to generalize the results of the experimental study.

• The Transactions of the Korea Information Processing Society
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• v.7 no.5
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• pp.1464-1473
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• 2000

In this paper, we analyze the cycle property of the (n,k)-star graph that has an attention as an alternative interconnection network topology in recent years. Based on the graph-theoretic properties in (n,k)-star graphs, we show the pancyclic property of the graph and also present the corresponding algorithm. Based on the recursive structure of the graph, we present such top-down approach that the resulting cycle can be constructed by applying series of "dimension expansion" operations to a kind of cycles consisting of sub-graphs. This processing naturally leads to such property that the resulting cycles tend to be integrated compactly within some minimal subset of sub-graphs, and also means its applicability of another classes of the disjoint-style cycle problems. This result means not only the graph-theoretic contribution of analyzing the pancyclic property in the underlying graph model but also the parallel processing applications such a as message routing or resource allocation and scheduling in the multi-computer system with the corresponding interconnection network.

• Journal of Microbiology and Biotechnology
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• v.14 no.1
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• pp.134-139
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• 2004

Hybrid cluster protein (HCP) was investigated because of its unique iron-sulfur clusters, which have been found in bacteria and archaea. Here, HCP homologous proteins from the third domain, 'eukarya'(3 amitochondriate protozoans, Giardia lamblia, Entamoeba histolytica, and Trichomonas vaginalis), were identified. All three amitochondriate protozoan HCPs (GlHCP, EhHCP, and TvHCP) belonged to Class I on the basis of two key characters, the cysteine spacing, Cys-(Xaa)₂Cys(Xaa)/sub 7-8/-Cys(Xaa)/sub 5/-Cys, and the absence of N-terminal deletion characteristic to the Class III. In phylogenetic analysis performed with amino acid sequences of 3 eukaryal, 5 bacterial, and 4 archaeal HCPs, the maximum likelihood (ML) tree indicated that TvHCP was clustered with Class I HCPs, whereas the other two HCPs (GlHCP and EhHCP) formed an independent clade with a high bootstrapping value (96%) not belonging to any previously recognized HCP class. In spite of the relatively lower bootstrapping value (61%), the position of the new eukaryal GlHCP-EhHCP clade was close to Class I, including the TvHCP, and Classes II and III were closely related with each other. The finding of eukaryal HCPs would help to understand the evolutionary history of HCP.

• Journal of Digital Convergence
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• v.18 no.1
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• pp.93-102
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• 2020

The purpose of this study is to analyze the effects of high school students' disability and academic stress on the participation of integrated physical education class. The result of this study is to examine the correlation between disability, academic stress and integrated physical education. There were negative correlations between parent stress and self stress among the subvariables. There was a positive correlation with the integrated physical education commitment and all the sub variables were positive. There was a negative correlation between academic stress and integrated physical education involvement, and all sub variables were negative.We examined the differences in the general characteristics of how disability and academic stress affect active commitment in the integrated physical education class. You can do it.