• Title/Summary/Keyword: study design

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Studies on the Environmental Factors Affecting the Cocoon Crops in Summer and Autumn in Korea (한국의 하추잠작 안정을 위한 환경요인에 관한 연구)

  • 이상풍
    • Journal of Sericultural and Entomological Science
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    • v.16 no.2
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    • pp.1-34
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    • 1974
  • These experiments pertain to various factors influencing the quantitative characters of cocoon crops in summer and early autumn seasons. Initially, in order to establish the possible ways of the silkworm rearing more than three times a year in Korea, the author attempted to get further information about the various factors affecting the cocoon crop in every silkworm rearing season. The trials were conducted eleven times a year at four places for three years. The field trial was conducted with 19 typical sericultural farmers who had been surveyed. At the same time the author statistically analyzed the various factors in close relation to tile cocoon crop in autumn season. The effect of guidance on 40 sericultural farmers was analyzed, comparing higher level farmers with lower level farmers ; and the author surveyed 758 non-guided farmers near the guided farmers during both spring and autumn seasons. In addition, another trial on the seasonal change of leaf quality was attempted with artificial diets prepared with leaves grown in each season. It was found that related factors to cocoon crops in summer and early autumn seasons appeared to be leaf quality, and temperature for young and grown larvae. A 2$^4$ factorial experiment was designed in summer season, and another design with one more level of varied temperature or hard leaf added to a 24 factorial experiment was conducted in early autumn. The experimental results can be summarized: 1. Study on the cocoon crops in the different rearing seasons 1) It was shown that earlier brushing of silkworm generally produced the most abundant cocoon crop in spring season, and earlier or later than the conventional brushing season, especially earlier brushing was unfavorable for the abundant cocoon crop in autumn season. 2) The cocoon crop was affected by the rearing season, and decreases in order of sire with spring, autumn, late autumn, summer and early autumn seasons. 3) It was Proved that ordinary rearing and branch rearing were possibles 4 times a year ; in the 1st, 3rd, 8th, and 10th brushing season. But the 11th brushing season was more favorable for the most abundant cocoon crop of branch rearing, instead of the 10th brushing season with ordinary rearing. 2. Study on the main factors affecting the cocoon crop in autumn season 1) Accumulated pathogens were a lethal factor leading to a bad cocoon crop through neglect of disinfection of rearing room and instruments. 2) Additional factors leading to a poor cocoon crop were unfavorable for rearing temperature and humidity, dense population, poor choice of moderately ripened leaf, and poor feeding techniques. However, it seemed that there was no relationship between the cocoon crop and management of farm. 3) The percentage of cocoon shell seemed to be mostly affected by leaf quality, and secondarily affected by the accumulation of pathogens. 3. Study on the effect of guidance on rearing techniques 1) The guided farms produced an average yearly yield of 29.0kg of cocoons, which varied from 32.3kg to 25.817g of cocoon yield per box in spring versus autumn, respectively. Those figures indicated an annual average increase of 26% of cocoon yield over yields of non-guided farmers. An increase of 20% of cocoon yield in spring and 35% of cocoon yield in autumn were responsible. 2) On guided farms 77.1 and 83.7% of total cocoon yields in the spring and autumn seasons, respectively, exceeded 3rd grade. This amounted to increases of 14.1 and 11.3% in cocoon yield and quality over those of non-guided farms. 3) The average annual cocoon yield on guided farms was 28.9kg per box, based on a range of 31.2kg to 26.9kg per box in spring and autumn seasons, respectively. This represented an 8% increase in cocoon yield on farms one year after guidance, as opposed to non-guided farms. This yield increase was due to 3 and 16% cocoon yield increases in spring and autumn crops. 4) Guidance had no effect on higher level farms, but was responsible for 19% of the increases in production on lower level farms. 4. Study on the seasonal change of leaf quality 1) In tests with grown larvae, leaves of tile spring crop incorporated in artificial diets produced the best cocoon crop; followed by leaves of the late autumn, summer, autumn, and early autumn crops. 2) The cocoon crop for young larvae as well as for grown larvae varied with the season of leaf used. 5. Study on factors affecting the cocoon crops in summer and early autumn A. Early autumn season 1) Survival rate and cocoon yield were significantly decreased at high rearing temperatures for young larvae 2) Survival rate, cocoon yield, and cocoon quality were adversely affected by high rearing temperatures for grown larvae. Therefore increases of cocoon quantity and improvement of cocoon quality are dependent on maintaining optimum temperatures. 3) Decreases in individual cocoon weight and longer larval periods resulted with feeding of soft leaf and hard leaf to young larvae, but the survival rate, cocoon yield and weight of cocoon shell were not influenced. 4) Cocoon yield and cocoon quality were influenced by feeding of hard leaf to grown larvae, but survival rate was not influenced by the feeding of soft leaf and hard leaf. 5) When grown larvae were inevitably raised at varied temperatures, application of varied temperature in the raising of both young and grown larvae was desirable. Further research concerning this matter must be considered. B. Summer season 1) Cocoon yield and single cocoon weight were decreased at high temperatures for young larvae and survival rate was also affected. 2) Cocoon yield, survival rate. and cocoon quality were considerably decreased at high rearing temperatures for grown larval stages.

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A Role for Leu247 Residue within Transmembrane Domain 2 in Ginsenoside-Mediated α7 Nicotinic Acetylcholine Receptor Regulation

  • Lee, Byung-Hwan;Choi, Sun-Hye;Pyo, Mi Kyung;Shin, Tae-Joon;Hwang, Sung-Hee;Kim, Bo-Ra;Lee, Sang-MoK;Lee, Jun-Ho;Lee, Joon-Hee;Lee, Hui Sun;Choe, Han;Han, Kyou-Hoon;Kim, Hyoung-Chun;Rhim, Hyewhon;Yong, Joon-Hwan;Nah, Seung-Yeol
    • Molecules and Cells
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    • v.27 no.5
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    • pp.591-599
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    • 2009
  • Nicotinic acetylcholine receptors (nAChRs) play important roles in nervous system functions and are involved in a variety of diseases. We previously demonstrated that ginsenosides, the active ingredients of Panax ginseng, inhibit subsets of nAChR channel currents, but not ${\alpha}7$, expressed in Xenopus laevis oocytes. Mutation of the highly conserved Leu247 to Thr247 in the transmembrane domain 2 (TM2) channel pore region of ${\alpha}7$ nAChR induces alterations in channel gating properties and converts ${\alpha}7$ nAChR antagonists into agonists. In the present study, we assessed how point mutations in the Leu247 residue leading to various amino acids affect 20(S)-ginsenoside $Rg_3$ ($Rg_3$) activity against the ${\alpha}7$ nAChR. Mutation of L247 to L247A, L247D, L247E, L247I, L247S, and L247T, but not L247K, rendered mutant receptors sensitive to $Rg_3$. We further characterized $Rg_3$ regulation of L247T receptors. We found that $Rg_3$ inhibition of mutant ${\alpha}7$ nAChR channel currents was reversible and concentration-dependent. $Rg_3$ inhibition was strongly voltage-dependent and noncompetitive manner. These results indicate that the interaction between $Rg_3$ and mutant receptors might differ from its interaction with the wild-type receptor. To identify differences in $Rg_3$ interactions between wild-type and L247T receptors, we utilized docked modeling. This modeling revealed that $Rg_3$ forms hydrogen bonds with amino acids, such as Ser240 of subunit I and Thr244 of subunit II and V at the channel pore, whereas $Rg_3$ localizes at the interface of the two wild-type receptor subunits. These results indicate that mutation of Leu247 to Thr247 induces conformational changes in the wild-type receptor and provides a binding pocket for $Rg_3$ at the channel pore.

A Study of Feasibility of Dipole-dipole Electric Method to Metallic Ore-deposit Exploration in Korea (국내 금속광 탐사를 위한 쌍극자-쌍극자 전기탐사의 적용성 연구)

  • Min, Dong-Joo;Jung, Hyun-Key;Park, Sam-Gyu;Chon, Hyo-Taek;Kwak, Na-Eun
    • Geophysics and Geophysical Exploration
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    • v.11 no.3
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    • pp.250-262
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    • 2008
  • In order to assess the feasibility of the dipole-dipole electric method to the investigation of metallic ore deposit, both field data simulation and inversion are carried out for several simplified ore deposit models. Our interest is in a vein-type model, because most of the ore deposits (more than 70%) exist in a vein type in Korea. Based on the fact that the width of the vein-type ore deposits ranges from tens of centimeters to 2m, we change the width and the material property of the vein, and we use 40m-electrode spacing for our test. For the vein-type model with too small width, the low resistivity zone is not detected, even though the resistivity of the vein amounts to 1/300 of that of the surrounding rock. Considering a wide electrode interval and cell size used in the inversion, it is natural that the size of the low resistivity zone is overestimated. We also perform field data simulation and inversion for a vein-type model with surrounding hydrothermal alteration zones, which is a typical structure in an epithermal ore deposits. In the model, the material properties are assumed on the basis of resistivity values directly observed in a mine originated from an epithermal ore deposits. From this simulation, we can also note that the high resistivity value of the vein does not affect the results when the width of the vein is narrow. This indicates that our main target should be surrounding hydrothermal alteration zones rather than veins in field survey. From these results, we can summarize that when the vein is placed at the deep part and the difference of resistivity values between the vein and the surrounding rock is not large enough, we cannot detect low resistivity zone and interpret the subsurface structures incorrectly using the electric method performed at the surface. Although this work is a little simple, it can be used as references for field survey design and field data Interpretation. If we perform field data simulation and inversion for a number of models and provide some references, they will be helpful in real field survey and interpretation.

Basic Studies on the Consumptive Use of Water Required for Dry Field Crops -Tomato and Chinese Cabbage- (밭작물소비수량에 관한 기초적 연구 -토마토 및 가을배추-)

  • 김철기;김진한;최홍규
    • Magazine of the Korean Society of Agricultural Engineers
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    • v.30 no.3
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    • pp.25-37
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    • 1988
  • The purpose of this study is to fmd out the bask data for irrigation plans of tomato and chinese cabbage during the growing period, such as total amount of evapotranspiration, coefficients of evapotranspiration at each growth stage, the peak stage of evapotranspiration, the maximum evapotranspiration, optimum irrigation point, total readily available moisture and intervals of irrigation date. The plots of experiment were arranged with split plot design which were composed of two factors, irrigation point for main plot and soji texture for split plot, and three levels, irrigation points with PF 1.8, PF 2.2, PF 2.6 for tomato and those with PF 1.9, PF 2.3, PF 2.7, for Chinese cabbage, soil textures of silty clay, sandy loam and sandy soil for both tomato and Chinese cabbage, with two replications. The results obtained are summarized as follows 1. There was the highest significant correlation between the evapotranspiration and the pan evaporation, beyond all other meteoralogical factors considered. Therefore, the pan evaporation is enough to be used as a meteorological index measuring the quantity of evapotranspiration. 2. 1/10 probability values of maximum total pan evaporation during growing period for tomato and Chinese cabbage were shown as 355.8 mm and 233.0 mm, respectively, and those of maximum ten day pan evaporation for tomato and Chinese cabbage, 68.0 mm and 43.8 mm, respectively. 3. The time that annual maximum of ten day pan evaporation can be occurred, exists at any stage of growing period for tomato, and at any growth stage till the late of Septemberfor Chinese cabbage. 4. The magnitude of evapotranspiration and of its coefficient for tomato and Chinese cabbage was occurred in the order of pF 1.8>pF 2.2>pF 2.6 and of pF 1.9>pF 2.3>pF 2.7 respectively in aspect of irrigation point and of silty clay>sandy loam>sandy soil in aspect of soil texture. 5. 1/10 probability value of evapotranspiration and its coefficient during the growing period of tomato were shown as 327.3 mm and 0.92 respectively, while those of Chinese cabbage, 261.0 mm and 1.12 respectively. 6. The time that maximum evapotranspiration of tomato can be occurred is at the date of fortieth to fiftieth after transplanting and the time for Chinese cabbage is presumed to he in the late of septemben At that time, 1/10 probability value of ten day evapotranspiration and its coefficient for tomato is presumed to be 74.8 mm and 1.10 respectively, while those of Chinese cabbage, 43.8 mm and 1.00. 7. In aspect of only irrigaton point, the weight of raw tomato and Chinese cabbage were mcreased in the order of pF 2.2>pF 1.8>pF 2.6 and of pF 1.9>pF 2.3>pF 2.7, respectively but optimum irrigation point for tomato and Chinese cabbage, is presumed to be pF 2.6 - 2.7 if nonsignificance of the yield between the different irrigation treatments, economy of water, and reduction in labour of irrigaion are synthetically considered. 8. The soil moisture extraction patterns of tomato and Chinese cabbage have shown that maximum extraction rate exists at 7 cm deep layer at the beginning stage of growth m any soil texture and that extraction rates of 21 cm to 35 cm deep layer are increased as getting closer to the late stage of growth. And especially the extraction rates of 21 cm deep layer and 35 cm deep layer have shown tendency to be more increased in silty clay than in any other soils. 9. As optimum irrigation point is presumed to be pF Z6-2.7, total readily available moisture of tomato in silty clay, sandy loam and sandy sofl becomes to be 19.06 mm, 21.37 mm and 20.91 mm respectively while that of Chinese cabbage, 18.51 mm, 20.27 mm, 21.11 mm respectively. 10. On the basis of optimum irrigation point with pF 2.6 - 2.7 the intervals of irrigation date of tomato and Chinese cabbage at the growth stage of maximum consumptive use become to be three days and five days respectively.

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Effects of Dietary Eucommia ulmoides Leaves on Nutrient Digestibility and Ruminal Fermentation Characteristics in Sheep (두충잎의 급여가 면양의 영양소 이용율과 반추위내 발효특성에 미치는 영향)

  • Kim, J.H.;Ko, Y.D.
    • Journal of Animal Science and Technology
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    • v.47 no.6
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    • pp.955-962
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    • 2005
  • This study was conducted to examine the nutrient digestibility and ruminal fermentation characteristics in sheep fed diets containing four substitution levels of E. ulmoides leaves for rice straw. For the experiment, they were given a basal diet consisting of rice straw and concentrate at a 3:7 ratio (DM basis). The treatments were designed as a 4$\time$4 Latin square design with four sheep (50.2$\pm$1.6 kg body weight). Sheep were allotted in one of four treatments, which were designed to progressively substitute Eucommia ulmoides for 0, 3, 5 and 10% of rice straw in the basal diet. The digestibility of crude fat was significantly (p<0.05) improved by 12.5 to 17.5% in 3% and 5% air dried Eucommia ulmoides leaves compared with that in control. The digestibility of crude fiber in the 3, 5 and 10% treatments was significantly (p<0.05) higher than that of the control. Dry matter intake was highest (p<0.05) in sheep fed 10% air dried E. ulmoides). Retained nitrogen in the 3, 5 and 10% treatments was increased up to 97%, 173% and 192%, respectively, compared to that in control (p<0.05). Organic matter utilization was higer in 3% and 5% treatments than control (p<0.05). Purine derivatives and microbial nitrogen synthesis were significantly increased by the dietary substitution of 5 % E. ulmoides leaves (p<0.05) compared with control and the 3% treatment. Ruminal concentration of total-VFA was significantly (p<0.05) higher in sheep fed all levels of air dried Eucommia ulmoides than those of control. It is concluded that air-dried E. ulmoides leaves given to ruminants can increase nutrient digestibility and palatability and improve ruminal fermentation. Therefore, the 5 and 10% substitution of E. ulmoides for roughage are highly recommended to be used in practice.

Effects of Fermented Wild-ginseng Culture by-products on Growth Performance, Blood Characteristics, Meat Quality and Ginsenoside Concentration of Meat in Finishing Pigs (발효산삼 배양액 부산물 급여가 비육돈의 생산성, 혈액성상, 육질특성 및 육내 Ginsenoside 함량에 미치는 영향)

  • Jang, H.D.;Kim, H.J.;Min, B.J.;Cho, J.H.;Chen, Y.G.;Yoo, J.S.;Lee, J.J.;Han, M.H.;Kim, In-Ho
    • Journal of Animal Science and Technology
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    • v.49 no.3
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    • pp.329-340
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    • 2007
  • This study was conducted to evaluate effects of fermented wild-ginseng culture by-product on growth performance, blood characteristics, meat quality and ginsenoside concentration of meat in finishing pigs. Forty-eight pigs(Landrace×Yorkshire×Duroc, 76.26±1.06kg average initial body weight) were used in 49d growth assay. Dietary treatments were included 1) CON(basal diet), 2) FWG1(basal diet+2.5% fermented wild-ginseng cultures by-product) and 3) FWG2(basal diet+5.0% fermented wild-ginseng cultures by-product). The pigs were allotted into four pigs per pen with four replicate pens per treatment by completely randomized design. No differences were found among treatments for ADG, ADFI and gain/feed from 0day to 49day of the experiment(P>0.05). Dry matter digestibility was greater in FWG1 treatment than CON treatment(P<0.05). In cholesterol concentration of blood, HDL cholesterol was significantly higher in CON treatment than FWG1 treatment(P<0.05). In meat quality, TBARS was significantly lower in FWG1 and FWG2 treatments than CON treatment(P<0.05). In sensory evaluation, Marbling was significantly higher in CON treatment than FWG1 treatment(P<0.05). Firmness in FWG2 and CON treatments was higher than FWG1 treatment(P<0.05). In meat color, L*-value of longissmus dorsi muscle was increased in FWG1 treatment compared to CON and FWG2 treatments(P<0.05). a* and b*-value of longissmus dorsi muscle were increased in CON and FWG1 treatments compared to FWG2 treatment (P<0.05). Ginsenoside concentration of meat was significantly higher in FWG2 treatment than CON treatment(P<0.05). In conclusion, fermented wild-ginseng culture by-product was effective for improving dry matter, TBARS, firmness, meat color and ginsenoside concentration of meat in finishing pigs.

Economics and Ground Cover Growth Characteristics of a New Method of Shallow Soil Artificial Foundation Planting (저토심 인공지반 녹화공법의 경제성 및 도입 가능한 지피식물의 생육특성)

  • Choi, Jin-Woo;Kim, Hag-Kee;Lee, Kyong-Jae;Kang, Hyun-Kyung
    • Journal of the Korean Institute of Landscape Architecture
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    • v.37 no.5
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    • pp.98-108
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    • 2009
  • The purpose of this study is to analyze the characteristics of limited methods, economics and breeding appropriateness of native and imported ground cover plants in the methodology of a shallow soil rooftop garden. The new shallow soil rooftop gardening method uses a total of 13cm in soil thickness, including 4.5cm of top soil on a 7.5cm rock-wool-mat stacked onto a 1cm roll-type-draining plate. The total construction cost for each method of soil level within the design price standard for SEDUM BLOCK is 89,433won/$m^2$, and for DAKU is 92,550won/$m^2$. By comparing those two methods, the construction cost of the shallow soil artificial foundation methodology is 45,000won/$m^2$; this shows the new method is 50% less expensive than the existing method of shallow soil rooftop gardening. The experiment was executed on the rooftop of the Korean National Housing Corporation to ensure validity of the shallow soil artificial foundation planting, and the sample plants which were imported and grown now in native covering. A list investigating the growing plants was made of the cover rate in each plant class, both while alive and the dry plant weight. The native ground cover plants, Sedum kamtschaticum, Sedum middendorffianum, Allium senescens, Sedum sarmentosum, Aquilegia buergariana, and Caryopteris incana increased the cover rate, live weight and dry weight in the shallow soil artificial foundation method. Among the imported cover plants, Sedum sprium and Sedum reflexum, the cover rate increased and growth conditions improved. However, some species needed weed maintenance. After examination with the less expensive shallow soil artificial foundation method and growth analysis, it was found that rooftop gardens are a low-cost option and the growth of plants is great. This result shows the new method can contribute to the proliferation of rooftop gardens in urban settings.

Multi-day Trip Planning System with Collaborative Recommendation (협업적 추천 기반의 여행 계획 시스템)

  • Aprilia, Priska;Oh, Kyeong-Jin;Hong, Myung-Duk;Ga, Myeong-Hyeon;Jo, Geun-Sik
    • Journal of Intelligence and Information Systems
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    • v.22 no.1
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    • pp.159-185
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    • 2016
  • Planning a multi-day trip is a complex, yet time-consuming task. It usually starts with selecting a list of points of interest (POIs) worth visiting and then arranging them into an itinerary, taking into consideration various constraints and preferences. When choosing POIs to visit, one might ask friends to suggest them, search for information on the Web, or seek advice from travel agents; however, those options have their limitations. First, the knowledge of friends is limited to the places they have visited. Second, the tourism information on the internet may be vast, but at the same time, might cause one to invest a lot of time reading and filtering the information. Lastly, travel agents might be biased towards providers of certain travel products when suggesting itineraries. In recent years, many researchers have tried to deal with the huge amount of tourism information available on the internet. They explored the wisdom of the crowd through overwhelming images shared by people on social media sites. Furthermore, trip planning problems are usually formulated as 'Tourist Trip Design Problems', and are solved using various search algorithms with heuristics. Various recommendation systems with various techniques have been set up to cope with the overwhelming tourism information available on the internet. Prediction models of recommendation systems are typically built using a large dataset. However, sometimes such a dataset is not always available. For other models, especially those that require input from people, human computation has emerged as a powerful and inexpensive approach. This study proposes CYTRIP (Crowdsource Your TRIP), a multi-day trip itinerary planning system that draws on the collective intelligence of contributors in recommending POIs. In order to enable the crowd to collaboratively recommend POIs to users, CYTRIP provides a shared workspace. In the shared workspace, the crowd can recommend as many POIs to as many requesters as they can, and they can also vote on the POIs recommended by other people when they find them interesting. In CYTRIP, anyone can make a contribution by recommending POIs to requesters based on requesters' specified preferences. CYTRIP takes input on the recommended POIs to build a multi-day trip itinerary taking into account the user's preferences, the various time constraints, and the locations. The input then becomes a multi-day trip planning problem that is formulated in Planning Domain Definition Language 3 (PDDL3). A sequence of actions formulated in a domain file is used to achieve the goals in the planning problem, which are the recommended POIs to be visited. The multi-day trip planning problem is a highly constrained problem. Sometimes, it is not feasible to visit all the recommended POIs with the limited resources available, such as the time the user can spend. In order to cope with an unachievable goal that can result in no solution for the other goals, CYTRIP selects a set of feasible POIs prior to the planning process. The planning problem is created for the selected POIs and fed into the planner. The solution returned by the planner is then parsed into a multi-day trip itinerary and displayed to the user on a map. The proposed system is implemented as a web-based application built using PHP on a CodeIgniter Web Framework. In order to evaluate the proposed system, an online experiment was conducted. From the online experiment, results show that with the help of the contributors, CYTRIP can plan and generate a multi-day trip itinerary that is tailored to the users' preferences and bound by their constraints, such as location or time constraints. The contributors also find that CYTRIP is a useful tool for collecting POIs from the crowd and planning a multi-day trip.

Effect of cyclic loading on axial displacement of abutment into implant with internal tapered connection: a pilot study (내측연결형 임플란트에 체결한 지대주의 수직침하에 대하여 반복하중이 미치는 영향)

  • Seol, Hyon-Woo;Heo, Seong-Joo;Koak, Jai-Young;Kim, Seong-Kyun;Han, Chong-Hyun
    • The Journal of Korean Academy of Prosthodontics
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    • v.51 no.4
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    • pp.315-322
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    • 2013
  • Purpose: To evaluate the axial displacement of implant-abutment assembly after cyclic loading in internal tapered connection system. Materials and methods: External butt-joint connection implant and internal tapered connection implant were connected with three types of abutment for cement-retained prostheses, i.e. external type abutment (Ext group), internal tapered 1-piece abutment (Int-1 group), and internal tapered 2-piece abutment (Int-2 group). For each group, 7 implants and abutments were used. The implantabutments assemblies were clamped into the implant holder for vertical loads. A dynamic cyclic loading was applied for $150{\pm}10N$ at a frequency of 4 Hz. The amount of axial displacement of the abutment into the implant was calculated at each cycle of 0, 5, 10, 50, 100, 1,000, 5,000, and 10,000. A repeated measures analysis of variance (ANOVA) for the overall effect of cyclic loading and the pattern analysis by linear mixed model were used for statistical analysis. Differences at P<.05 were considered statistically significant. Results: The mean axial displacement after 10,000 cycles were $0.714{\pm}0.488{\mu}m$ in Ext group, $5.286{\pm}1.604{\mu}m$ in Int-1 group, and $11.429{\pm}1.902{\mu}m$ in Int-2 group. In the pattern analysis, Int-1 and Int-2 group showed continuous axial displacement at 10,000 cycles. There was no declining pattern of axial displacement in the Ext group. Conclusion: The pattern of linear mixed model in Ext group showed no axial displacement. There were continuous axial displacements in abutment-implant assemblies in the Int-1 and Int-2 group at 10,000 cycles. More axial displacement was found in Int-2 group than in Int-1 group.

Improvement of Seed Germination in a Spontaneous Autotetraploid of Poncirus and Chlorophyll Fluorescence of Seedlings in Salt Stress (동질 사배체 탱자의 종자 발아 증진과 염류 과잉에 따른 엽록소 형광 반응)

  • Chae, Chi Won;Yun, Su Hyun;Park, Jae Ho;Kim, Min Ju;Han, Seung Gab;Kang, Seok Beom;Koh, Sang Wook;Han, Sang Heon
    • Journal of Life Science
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    • v.23 no.9
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    • pp.1079-1087
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    • 2013
  • Speed germination success and robust vegetative growth of citrus rootstock through improved sowing methods and fertilizer inputs offer the usage of root system for the citrus. The current study evaluated the influence of seed coat removal and different fertilizer concentrations on plant germination and plant growth of spontaneous rootstock siblings. Decoated and coated seeds of diploid and tetraploid plants were sown in tubes. Commercial fertilizer concentrations of 0, 2, 4, 6, 8 and $10g{\cdot}l^{-1}$ were added. The experimental layout followed a randomized block $2{\times}6$ factorial design (seed coat removal ${\times}$ fertilizer concentration) for each rootstock. Fertilizer concentrations were 0, 10, 20 and $30g{\cdot}l^{-1}$ of the fertilizer for the resistance of the strength on the salt level. The germination rate of seeds without testa sown in vitro was improved (67-80%) compared to that of nontreated seeds. The eventual tree height of the seeds without testa in the diploid group was increased due to higher fertilization compared to that in the nontreated group. The removal of seed testa promoted the seed germination of both diploid and tetraploid trifoliate orange and resulted in greater height. Their vegetative development was also increased due to the increased fertilization of the rootstock. The Fv/Fm value for the diploid plants was 0.4 and 0.8 for the tetraploid ones under salt stress after 11 days of treatment. The removal of seed testa may improve the seed germination of trifoliate orange. Tetraploid trifoliate orange appears to possess resistance to salt stress compared to the diploid variety.