• Proceeding of EDISON Challenge
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• 2016.03a
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• pp.90-95
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• 2016

Heat Shock 70kDa Protein 1-Like(HSPA1L)는 Heat-shock protein70(HSP70) family에 속하는 chaperone protein으로 polypeptide folding, assembly, protein degradation 등 다양한 biological processes에 관여하고 있다. HSPA1L은 human methyltransferase-like protein 21A(METTL21A)에 의해 lysine residue에 methylation이 일어나게 되는데, 암세포에서 일반적인 HSPA1L은 주로 세포질에서 발견되는 반면 methylated HSPA1L의 경우 주로 핵에서 발견이 됨으로써 HSPA1L methylation이 암 세포 성장에 중요할 역할을 할 것이라 추측되며 anti-cancer drug target으로 주목 받고 있다. 하지만 현재 HSPA1L의 구조가 부분적으로만 밝혀져 있어 HSPA1L와 METTL21A가 어떤 residue들이 interaction 하여 binding을 하는지에 대해서 아직 밝혀 지지 않았다. 이로 인해 anti-cancer drug target으로서의 연구에 제한이 있다. 이번 연구에서는 homology modeling(Galaxy-TBM, Galaxy-refine)을 통해 HSPA1L 전체 구조를 밝혀 낸 후, HSPA1L 와 METTL21A를 protein-protein docking을 통해 binding pose 예측을 하였다. 이러한 binding pose를 protein interaction analysis하여 HSPA1L과 METTL21A binding에 관여하는 중요 residue들을 밝혀 냈다. 이러한 structural information은 methylated HSPA1L와 암 세포 성장간의 연관성, 더 나아가 anti-cancer drug 개발로 까지도 이어 질 수 있을 것이라 생각한다.

• Journal of Microbiology and Biotechnology
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• v.18 no.7
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• pp.1335-1341
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• 2008

The cell wall material from fruiting bodies of Laetporus sulphureus has been suggested as a new alternative to mutan for the mutanase induction in Trichoderma harzianum. Structural analyses revealed that the cell wall fraction from this polypore fungus contained 56.3% of (1$\rightarrow$3)-linked $\alpha$-glucans. When the strain T. harzianum F-340 was grown on a cell wall preparation from L. sulphureus, the maximal enzyme productivity obtained after 3 days of cultivation was 0.71 U/ml. This yield was about 1.8-fold higher than that achieved on mutan, known so far as the best, but expensive and inaccessible, inducer of mutanase production. Cell-wall-induced mutanase showed a high hydrolytic potential in reaction with a dextranase-pretreated mutan, where maximal degrees of saccharification and solubilization of this biopolymer (80% and 100%, respectively) were reached in 3 h at 45$^{\circ}C$. The mutanase preparation was also effective in degradation of streptococcal mutan and its removal from oral biofilms, especially in a mixture with dextranase.

• Natural Product Sciences
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• v.13 no.4
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• pp.289-294
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• 2007

Ethanolic extract (50% v/v) and alkaloid fraction of Tylophora indica leaves were examined for lysosomal enzyme inhibitory activity in adjuvant-induced arthritic rats. The alkaloid fraction showed statistically significant inhibition of arthritic lesions (p < 0.05) from day 18, (p < 0.025) from day 20 and (p < 0.001) from day 21 onwards in the adjuvant-induced arthritis, which was comparable to the response of standard drug Indomethacin. The ethanolic extract was less significant than the alkaloidal fraction in inhibition of arthritis. Alkaloid fraction showed significant (p < 0.001) inhibitory effect on the lysosomal enzyme activities in adjuvantinduced arthritic rats. It also significantly prevented decrease in collagen levels and synovial damage observed during arthritis and also inhibited increase in urinary excretion levels of collagen degradation products like hydroxyproline, hexosamine, hexuronic acid, etc. Both ethanolic extract as well as the alkaloid fraction, however, did not show any significant activity in normal nonarthritic rats. The ethanolic extract and the alkaloid fraction may thus be able to inhibit the progress of inflammation and inhibit the destructive activity of lysosomal enzymes on structural macromolecules like collagen etc. in the synovial capsule in joints during arthritic states. They may thus prevent synovial damage observed during arthritis.

• Journal of Power System Engineering
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• v.10 no.1
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• pp.96-101
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• 2006

In ship operation the consequence of roll and pitchingmotion can seriously degrade the performance of mechanical and personnel effectiveness. So many studies for the roll stabilization and trimming control system design have been performed and good results have been achieved where the stabilizing fins, tanks, rudders and flaps are used. However the ultimate objective of such approach should be focused on improving the boarding sensitivity. But there may exist many unsolved problems, for examples, ship control performance degradation and increasing of system complexity. So, the achieved control performance could not give us enough comfortable boarding sensitivity where the residual rolling and pitching motion are main drawbacks. To get rid of these disadvantages, the main hull control systems design approach has been considered using semiactive absorber. In this system, dampers, spring, dynamic dampers and control system with sensors are incorporated. In our system considered in this study, just two motors and control system with sensors are used for the bed. And the control system can be installed on each bed. So, we can control every bed on the specified control objective respectively. Above all, the good advantages of this system are the facts followed from simple idea and usefulness. Of course the structural modifications are needed. Considering disturbances, we design control system and verify the usefulness of developed system from the experimental study.

• Proceedings of the Korean Society of Applied Pharmacology
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• 2007.11a
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• pp.129-139
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• 2007

Many modalities of treatment for acquired skin hyperpigmentation are available including chemical agents or physical therapies, but none are completely satisfactory. The ideal depigmenting compound should have a potent. rapid and selective bleaching effect on hyperactivated melanocytes, carry no short- or long-term side-effects and lead to a permanent removal of undesired pigment. acting at one or more steps of the pigmentation process. Depigmentation can be achieved by regulating (i) the transcription and activity of tyrosinase, tyrosinase related protein-1 (TRP-1), tyrosinase related protein-2 (TRP-2), and/or peroxidase; (ii) the uptake and distribution of melanosomes in recipient keratinocytes and (iii) melanin and melanosome degradation and turnover of pigmented keratinocytes. One of the interesting point for development of skin whitening agent is Mitf(Microphthalmia-associated transcription factor). Mitf belongs to the basic helix-loop-helix-zip family of trabscription factors and it is crucial as it regulates both melanocyte proliferation as well as melanogenesis and is the major regulator of tyrosinase and the related enzymes (TRPs), as well as many melanosome structural proteins such as pMel17. Recently, we developed MITF-down-regulating agents from natural and synthetic sources, which have anti-melanogenic effect on in vitro and in vivo. We suggested that potent MITF-down regulating agents might be used for skin whitening cosmeceuticals.

• 한국신재생에너지학회:학술대회논문집
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• 2006.06a
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• pp.145-148
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• 2006

The protocrystalline silicon (pc-Si:H) multilayer solar cell is very promising owing to its fast stabilization with low degradation against light irradiation. However, the pc-Si:H multi layers have not extensively been investigated in detail on its material characteristics yet. We present the material characteristics of pc-Si:H multilayer using a transmission electron microscopy(TEM), and Raman spectroscopy. In addition, we present the superior light-soaking behavior of the pc-Si:H mutt i layer solar cell. A TEM micrograph shows that a pc-Si:H multilayer has a repeatedly layered structure and crystalline-like objects in a-Si:H matrix. A Raman spectra introduces improved short-range-order and medium-range-order in pc-Si:H multilayer. As a result the excellent metastability of the pc-Si:H multilayer solar cell is primarily due to the repeatedly layered structure that improves a structural order in absorber layer.

• Journal of Surface Science and Engineering
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• v.49 no.4
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• pp.344-348
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• 2016

Stainless steel is widely applied in many industrial fields due to its excellent anti-corrosion and durability characteristics. However, stainless steel is very vulnerable to cavitation attack caused by high speed flow of fluid in the chloride environments such as marine environment. These conditions promote intergranular corrosion and cavitation-erosion, leading to degradation of the structural integrity and service life. In order to prevent these problems, the stabilized stainless steel is applied to the offshore and shipbuilding industries. In this study, Ti was added to 19%Cr-9%Ni as the stabilizer element with different concentrations (0.26%, 0.71%), and their durabilities were evaluated with cavitation-erosion experiment by a modified ASTM G32 method. The microstructural change was observed with the stabilizer element contents. The result of the observation indicated that the amount of carbide precipitation was decreased and its size became finer with increasing Ti content. In the cavitation-erosion experiment, both weight loss and surface damage depth represented an inverse proportional relationship with the amount of Ti element. Consequently, the stainless steel containing 0.71% of Ti had excellent durability characteristics.

• Smart Structures and Systems
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• v.25 no.3
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• pp.265-277
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• 2020

Recently, the concept of "drive-by" bridge monitoring system using indirect measurements from a passing vehicle to extract key parameters of a bridge has been rapidly developed. As one of the most key parameters of a bridge, the natural frequency has been successfully extracted theoretically and in practice using indirect measurements. The frequency of bridge is generally calculated applying Fast Fourier Transform (FFT) directly. However, it has been demonstrated that with the increase in vehicle velocity, the estimated frequency resolution of FFT will be very low causing a great extracted error. Moreover, because of the low frequency resolution, it is hard to detect the frequency drop caused by any damages or degradation of the bridge structural integrity. This paper will introduce a new technique of bridge frequency extraction based on Hilbert Transform (HT) that is not restricted to frequency resolution and can, therefore, improve identification accuracy. In this paper, deriving from the vehicle response, the closed-form solution associated with bridge frequency removing the effect of vehicle velocity is discussed in the analytical study. Then a numerical Vehicle-Bridge Interaction (VBI) model with a quarter car model is adopted to demonstrate the proposed approach. Finally, factors that affect the proposed approach are studied, including vehicle velocity, signal noise, and road roughness profile.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.11 no.8
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• pp.1589-1595
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• 2007

Recently many communication systems ate adapting the OFDM(Orthogonal Frequency Division Multiplexing) for multimedia data services. However, OFDM has some problems that PAPR is increased when sub-carries are summed up with same phases. This problem causes severe signal distortions while the signal is passing through non-linear system such as power amplifier resulting in performance degradation of transmission system. In this paper, we use IF-Clipping method to solve PAPR problem and the signal distortion caused by clipping is limited by use of SAW (Surface Acoustic Wave) filter and power amplifier(TWTA). And we derive the optimal parameter(PAPR, Backoff) values to meet the spectrum mask of WiBro system.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2012.05a
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• pp.117-122
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• 2012

A blisk with rotor shroud is usually adopted in LRE turbine to maximize its performance. However it experiences severe thermal load and resulting damage during engine stating and stop. Shroud splitting is devised to relieve thermal stress on the turbine rotor. Structural analysis confirmed the reduction of plastic strain at the blade hub and tip. However, split gap at the rotor shroud entails additional tip leakage and results performance degradation. In order to assess the effect of shroud split on the turbine performance, tests have been performed for various settings of shroud split. For the maximum number of shroud splitting, measured efficiency reduction ratio was 2.65% to the value of original shape rotor.