• 동의생리병리학회지
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• 제21권1호
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• pp.117-125
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• 2007

Neuronal ischemia is a pathological process caused by a lack of oxygen (anoxia) and glucose (hypoglycemia), resulting in neuronal death. It is believed that apoptosis is one of the mechanisms involved in ischemic cell death. Neuronal apoptosis is a process characterized by nuclear DNA fragmentation, changes of plasma membrane organization. To elucidate the mechanism of neuronal death following ischemic insult and to develop neuroprotective effects of Daebowonjeon(DBWJ) against ischemic damage, in vitro models are used. In vitro models of cell death have been devloped with pheochromocytoma (PC12) cell, which have become widely used as neuronal models of oxidative stress, trophic factor, serum deprivation and chemical hypoxia. Using a special ischemic device and PC12 cultures, we investigated an in vitro model of ischemia based on combined Oxygen and Glucose Deprivation (OGD) insult, followed by reoxygenation, mimicking the pathological conditions of ischemia. In this study, Daebowonjeon rescued PC12 cells from Oxygen-Glucose Deprivation (OGD)-induced cell death in a dose-dependent manner The nuclear staining of PC12 cells clearly showed that DBWJ attenuated nuclear condensation and fragmentation which represent typical neuronal apoptotic characteristics. DBWJ also prevents the LDH release and induction of Hypoxia Inducing Factor (HIF)-1 by OGD-exposed PC12 cells. Furthermore, DBWJ reduced the activation of polyADP-ribose polymerase (PARP) by OGO-exposed PC12 cells. These results suggest that apoptosis is an important characteristic of OGD-induced neuronal death and that oriental medicine, such as DBWJ, may prevent PC12 cell from OG D-induced neuronal death by inhibiting the apoptotic process.

• 마이크로전자및패키징학회지
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• 제23권2호
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• pp.11-18
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• 2016

This study focused on examining the possibility for recycling of Fe-Si electric sheet. We manufactured Fe-6.5Si mother alloy using by Fe-Si electric sheet scrap for transformer core materials. And then, soft magnetic alloy powder which diameter and shape were $45{\sim}150{\mu}m$ and sphere type was prepared by gas atomization process. As we compared to commercial Fe-6.5Si powder, its diameter distribution and microstructure of recycled powder was a similar. To investigate the possibility of reusing the soft magnetic composite sheet for electronics, recycled powder was treated to have a high aspect ratio (AR), and we finally obtained the 65~66 AR and $2.3{\mu}m$ thickness powder. To release the residual stress of powder, heat treatment was conducted under $300{\sim}400^{\circ}C$, $N_2$ gas. And then, soft magnetic sheet was made by tape casting process using by those powders. After the density and permeability of tape was measured, and we confirmed that the recycled Fe-Si electric sheet scrap was possible to reuse the soft magnetic materials of electronics.

• 한국표면공학회지
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• 제48권2호
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• pp.33-37
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• 2015

High efficiency LED device is being concerned due to its high heat loss, and such heat loss will cause a shorter lifespan and lower efficiency. Since there is a demand for the materials that can release heat quickly into the external air, the organic insulating layer was required to be replaced with high thermal conductive materials such as metal or ceramics. Through anodizing the upper layer of Al, the Breakdown Voltage of 3kV was obtained by using an uniform thickness of $60{\mu}M$ aluminum oxide($Al_2O_3$) and was carried out to determine the optimum process conditions when thermal cracking does not occur. Two Ni layers were formed above the layer of $Al_2O_3$ by sputtering deposition and electroplating process, and saccharin was added for the purpose of minimizing the remain stress in electroplating process. The results presented that the 3-layer film including the Ni layer has an adhesive force of 10N and the thermal conductivity for heat dissipation is achieved by 150W/mK level, and leads to improvement about 7 times or above in thermal conductivity, as opposed to the organic insulation layer.

• 한국지반신소재학회논문집
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• 제12권4호
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• pp.133-141
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• 2013

본 연구에서는 시울지역 내 분포하고 있는 15개 지역의 문화재 주변지반에 대하여 표면파탐사를 바탕으로 하는 지반조사결과를 이용하여 수치해석 기반의 3차원 내진해석을 수행하였으며, 해석결과를 기반으로 지반변위를 분석하였다. 해석결과, 지반의 변위는 (구)공업전습소 본관이 최대 약 9.7mm로 나타났다. 이와 같이 변위가 작게 나타난 원인은 문화재가 위치한 지역의 토층이 매우 단단하기 때문으로 표면파 탐사를 통해서 분석된 지반가속도를 비교해 보아도 하부에 매우 단단한 암층이 존재하는 것으로 확인되었다. 그러나 낙성대 삼층석탑에 있어서, 지반 자체의 변위는 다른 지역과 유사하게 나타났으나 화강암으로 구성된 석탑은 상부에서 최대 30mm의 변위를 나타냈다. 이는 낙성대 삼층석탑을 지지지하는 지반의 경우 지진파에 대한 상호 보완을 할 수 있는 구조, 즉 지반반력으로 인한 감쇠효과가 나타나기 때문에 지반의 변위 자체는 작게 해석되었으나 상부 구조물의 경우에는 지진파가 작용하였을 때 응력해방에 기인하여 변위가 가장 많이 발생한 것으로 판단된다.

• 대한치과보철학회지
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• 제36권1호
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• pp.120-132
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• 1998

This stusy was to investigate the marginal fitness of porcelain-fused-to- metal crown after succesive firing cycle. Main variables were the degree of marginal curvature of labiocervical margin and the type of alloy. The exaggerated marginal curvature(EMC) was created by additional reduction at the faciocervical wall of the normallized marginal curvature (NMC)-typed ivorine tooth by using milling machine. The difference in the shape was the mid facial margin was placed 2mm apical to cemento- enamel junction in labial surface. Three types of alloy were high noble, noble, and base metal alloy. Test specimens were divided into 8 groups and each group had 8 specimens. Sixty four ceramometal crowns were made totally. Measurement stages were following degassing, opaquing. body porcelain firing, and glazing, and measuring sites were 4. (midmesial, midfacial, middistal, and midlingual). Digital, travelling measuring microscope (0.5 um precision, Olympus. Japan) was used under ${\times}250$ magnification. Within the limitation of this investigation, it was concluded as belows: 1. The pattern of marginal distortion was varied. Degassing stage was not a specific, causative stage that induce most of total marginal distortion during whole procedure fabricating a ceramometal crown. Body firing stage induced discrepancy relatively more than other firing stages. 2. The specimens that were Ni-based alloy and had EMC were distorted persistently following successive fabricating procedures. But marginal openings were decreased after glazing. 3. The release of metal grinding-induced stress was presumed as a cause that induce marginal distortion. 4. The amount of discrepancies of the labial and lingual margins were greater than that of the mesial and distal margin in the specimen that had EMC. 5. Silver-plated die was not enough to resist abrasion during repeated seating of metal copings on the die-holding device.

• IMMUNE NETWORK
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• 제13권4호
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• pp.141-147
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• 2013

Hypoxia has been shown to promote inflammation, including the release of proinflammatory cytokines, but it is poorly investigated how hypoxia directly affects inflammasome signaling pathways. To explore whether hypoxic stress modulates inflammasome activity, we examined the effect of cobalt chloride ($CoCl_2$)-induced hypoxia on caspase-1 activation in primary mixed glial cultures of the neonatal mouse brain. Unexpectedly, hypoxia induced by oxygen-glucose deprivation or $CoCl_2$ treatment failed to activate caspase-1 in microglial BV-2 cells and primary mixed glial cultures. Of particular interest, $CoCl_2$-induced hypoxic condition considerably inhibited NLRP3-dependent caspase-1 activation in mixed glial cells, but not in bone marrow-derived macrophages. $CoCl_2$-mediated inhibition of NLRP3 inflammasome activity was also observed in the isolated brain microglial cells, but $CoCl_2$ did not affect poly dA:dT-triggered AIM2 inflammasome activity in mixed glial cells. Our results collectively demonstrate that $CoCl_2$-induced hypoxia may negatively regulate NLRP3 inflammasome signaling in brain glial cells, but its physiological significance remains to be determined.

• Journal of Acupuncture Research
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• 제18권3호
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• pp.123-133
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• 2001

Objective : This study was undertaken to determine whether Cordyceps sinensis Sacc. (CSS) extract exerts the protective effect against oxidant-induced alterations in membrane transport function in renal tubules. Methods : Membrane transport fucntion was estimated by examining alterations in p-aminohippurate (PAH) uptake in rabbit renal cortical slices. For induction oxidative stress, slices were treated with an organic peroxide cumene hydroperoxide for 60 min at $37^{\circ}C$. Cumene hydroperoxide inhibited PAH uptake in a time dependent manner. Results : CSS at 0.5-5% concentrations prevented cumene hydroperoxide-induced inhibition of PAH uptake. CSS at 1% also attenuated LDH release and lipid peroxidation induced by cumene hydroperoxide. When slices were treated with 0.2 mM mercury chloride, PAH uptake was inhibited and lipid peroxidation was increased. These changes by mercury were significantly prevented by CSS. Conclusion : These results suggest that CSS prevents oxidant-induced alterations in membrane transport function in rabbit renal cortical slices. Such protective effect of CSS may be attributed to inhibition of peroxidation of membrane lipid.

• 한국식품영양학회지
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• 제30권6호
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• pp.1279-1285
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• 2017

$Amyloid-{\beta}$ protein ($A{\beta}$) is known to increase free radical production in neuronal cells, leading to cell death by oxidative stress. The purpose of this study was to evaluate the protective effects of $PineXol^{(R)}$ on $A{\beta}_{25-35}$ induced neuronal cell death. Rat pheochromocytoma (PC-12) cells were pre-treated with $100{\mu}g/mL$ of $PineXol^{(R)}$ for 2 h. The cells were exposed to single dose of $30{\mu}M$ $A{\beta}_{25-35}$ for 24 h. Cell death was assessed by a cell count kit-8 (CCK-8) assay, lactate and dehydrogenase (LDH) release assay. An Apoptotic process was analyzed by a protein expression of the Bcl-2 family using western blotting. Cell viability increased in PC-12 cells treated with both $A{\beta}_{25-35}$ and $PineXol^{(R)}$, compared to the control group. $PineXol^{(R)}$ induced a decrease of the Bcl-2 protein expression (p<0.05), while Bax and Sod1 increased (p<0.05), indicating attenuation of $A{\beta}_{25-35}$ induced apoptosis. These results suggest that $PineXol^{(R)}$ may be a good candidate for the prevention of Alzheimer's disease(AD).

• 한국수산과학회지
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• 제27권6호
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• pp.690-695
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• 1994

혐기-호기 체계의 활성오니를 이용하여 폐수로부터 인과 질소를 동시에 제거하는 연구를 행하였다. 혐기적인 조건에서 nitrate 혹은 nitrite의 농도가 거의 0일 때, 제거된 glucose의 양은 orthophosphate의 방출과 직선적인 관계를 나타내었으며, 유입수에서 인과 glucose의 비율이 0.04 이하일 때, 폐수 중의 인은 호기적인 단계에서 거의 제거되었다. 이는 혐기적 단계에서 받은 stress로 인해 호기적 단계에서 받은 stress로 인해 호기적 단계에서 훨씬 많은 양의 인을 흡수한 것으로 생각된다. 혐기적 단계에서의 인의 방출양과 호기적 단계에서의 인의 흡수양은 $NO_x-N$의 농도와는 비례적인 관계가 아니었으며, 더우기, glucose에 대한 $NO_2-N$의 비가 0.37보다 낮을 때, 유입수 중의 무기질소는 완전히 제거되었다.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1997년도 춘계학술대회
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• pp.85-85
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• 1997

스트레스가 유발된 랫드의 대뇌에서 Vasopressin-catecholamine pathway의 활성도를 알아보기 위해 면역화학염색법으로 vasopressin 호르몬의 분비와 catecholamine의 생성변화를 tyrosine hydroxylase (TH) 효소의 발현변화로 규명하고, arginine vasopressin (AVP)과 V1 vasopressin receptor의 유전자 발현변화를 in situ hybridization 방법을 이용하여 살펴보았다. 수컷 SD rat를 7시간동안 stress cage에 넣어 16$\pm$1$^{\circ}C$의 물에 수침구속 스트레스를 준 후 대조군과 함께 관류고정하여 brain을 적출하였다. Brain의 hypothalamus 부위를 중심으로하여 동결절편하여 면역조직화학 염색과 in situ hybridization을 시행하였다. TH 면역조직화학 염색에서 대뇌의 줄무늬체 부위의 꼬리조가비핵에서와 시상하부 부위의 내측등쪽시상하부와 흑색질부위에서 스트레스군이 대조군에 비해 TH 면역염색성이 증가되어 관찰되었으나 시상하부 부위의 시삭위핵, 뇌실주위핵, 뇌실옆핵에서는 두 군간의 큰 면역염색성의 차이는 보이지 않았다. AVP 면역조직화학 염색에서는 시삭위핵에 많은 수의 AVP 양성 신경세포체들이 밀집되어 있으며 뇌실옆핵에서는 스트레스군에서 AVP 면역염색성이 약간 증가되어 관찰되었으나 신경섬유의 분포양상은 비슷하였다. 중간융기에서는 모두 강한 염색성의 신경섬유들이 관찰되어 두 군간에 큰 차이는 없었다. AVP 유전자에 대한 in situ hybridization 결과 시삭위핵의 신경세포에서 AVP mRNA 양성반응을 관찰할 수 있었으나 다른 시상하부핵에서는 관찰할 수 없었으며, V1 vasopressin receptor에 대한 in situ hybridization 결과는 두 군의 대뇌에서 모두 양성반응을 관찰할 수 없었으며 V1 vasopressin receptor 유전자의 조직별 발현정도와 스트레스에 의한 발현량 조절을 관찰할 필요가 있다고 사료된다.