• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 춘계학술발표대회
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• pp.355-356
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• 2000

Three 'stress probe' plasmids were constructed and characterized which utilize a green fluorescent protein (CFP) as a non-invasive reporter to elucidate Escherichia coli cellular stress responses in quiescent or 'resting' cells. Facile detection of cellular stress levels was achieved by fusion of three heat shock stress protein promoter elements, those of the heat shock transcription factor ${\sigma}^{32}$, pretense subunit ClpB, and chaperone DnaK, to the reporter gene $gfp_{uv}$. When perturbed by chemical or physical stress (such as heat shock, nutrient (amino acid) limitation, addition of IPTG, acetic acid, ethanol, phenol, antifoam, and salt (osmotic shock), the E. coli cells produced GFPuv which was easily detected from within the cells as emitted green fluorescence. A temporal and amplitudinal mapping of these responses was performed, demonstrating regions where quantitative delineation of cell stress was afforded.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2004년도 추계학술대회
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• pp.7-12
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• 2004

A lifetime prediction of holddown spring screw in nuclear fuel assembly was performed using fracture mechanics approach. The spring screw was designed such that it was capable of sustaining the loads imposed by the initial tensile preload and operational loads. In order to investigate the cause of failure and to predict the stress corrosion cracking life of the screw, a stress analysis of the top nozzle spring assembly was done using finite element analysis. The elastic-plastic finite element analysis showed that the local stresses at the critical regions of head-shank fillet and thread root significantly exceeded than the yield strength of the screw material, resulting in local plastic deformation. Normalized stress intensity factors for PWSCC life prediction was proposed. Primary water stress corrosion cracking life of the Inconel 600 screw was predicted by using integration of the Scott model and resulted in 1.78 years, which was fairly close to the actual service life of the holddown spring screw.

• Journal of Welding and Joining
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• 제8권3호
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• pp.79-88
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• 1990

Welded joint often contains soft or softened regions such as the HAZ of TMCP steel welded with high heat input. In this study, the equivalent yield strength of plate structure containing softened region was predicted by FEM analysis, and its incremental behavior was explained with the results of the analysis. The calculated results of yield strength indicated the following for the plate structures. 1) As the softened region starts to yield, shear stress begins to build up along the boundary between base metal and softened region. This results in multi-axial stress condition which gives restraint on the softened region. 2) Restraint effect has a significant influence on the distribution of the shear stress, the nominal stress, and the strain. 3) The yielding behavior of softened region becomes the same as that of base metal when both ratios of length to width and thickness to width of softened region are larger than 30 and 13 respectively.

• 대한의용생체공학회:의공학회지
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• 제13권3호
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• pp.253-262
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• 1992

The wall shear stress in the vicinity of end-to end anastomoses under steady flow condi- tions was measured using a flush-mounted hot-film anemometer(FMHFA) probe. The experi- mental measurements were in good agreement lith numerical results except In flow with low Reynolds numbers. The wall shear stress increased proximal to the anastomosis in flow from the Penrose tubing (simulating an artery) to the PTFE graft. In flow from the PTFE graft to the Penrose tubing, low wall shear stress was observed distal to the anastomosis. Abnormal distributions of wall shear stress in the vicinity of the anastomosis, resulting from the compli- ance mismatch between the graft and the host artery, might be an important factor of ANFH formation and the graft failure. The present study suggests a correlation between regions of the low wall shear stress and the development of anastomotic neointimal fibrous hyperplasia (ANFH) in end-to-end anastomoses.

• Earthquakes and Structures
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• 제18권3호
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• pp.285-296
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• 2020

Dams are important structures for management of water supply for irrigation or drinking, flood control, and electricity generation. In seismic regions, the structural safety of concrete gravity dams is important due to the high potential of life and economic loss if they fail. Therefore, the seismic analysis of existing dams in seismically active regions is crucial for predicting responses of dams to ground motions. In this paper, earthquake response of concrete gravity dams is investigated using the finite element (FE) method. The FE model accounts for dam-water-foundation rock interaction by considering compressible water, flexible foundation effects, and absorptive reservoir bottom materials. Several uncertainties regarding structural attributes of the dam and external actions are considered to obtain the fragility curves of the dam-water-foundation rock system. The structural uncertainties are sampled using the Latin Hypercube Sampling method. The Pine Flat Dam in the Central Valley of Fresno County, California, is selected to demonstrate the methodology for several limit states. The fragility curves for base sliding, and excessive deformation limit states are obtained by performing non-linear time history analyses. Tensile cracking including the complex state of stress that occurs in dams was also considered. Normal, Log-Normal and Weibull distribution types are considered as possible fits for fragility curves. It was found that the effect of the minimum principal stress on tensile strength is insignificant. It is also found that the probability of failure of tensile cracking is higher than that for base sliding of the dam. Furthermore, the loss of reservoir control is unlikely for a moderate earthquake.

• 한국간호교육학회지
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• 제20권1호
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• pp.47-59
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• 2014

Purpose: The purpose of this study was to review articles and theses on Korean nursing students' stress, identifying overall research trends regarding such stress and suggesting strategies for stress management and nursing education system. Methods: The subjects for this study were 31 articles selected from three databases according to specific inclusion criteria. Results: Twenty-seven articles described survey research and four articles reported on experiments. Twenty-two studies related to stress from clinical practice among nursing students. Thirty-one articles reported on 40 regions and 44 colleges. Seventeen types of tools were used to measure nursing students' stress. The reported reliability of tools ranged from .76 to .96, and the mean score for nursing students' stress fell between 1.98 and 3.95. Conclusion: The findings from this review article indicate that nursing students exhibit different levels of stress as a result of school. Thus, they must learn to cope with stress to support their physical and psychological health. Moreover, finding ways to help student nurses handle job-related stress will require research related to the overall school, the curriculum, learning, and the life of nursing students, as well as stress intervention.

• 대한한방내과학회지
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• 제34권3호
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• pp.256-266
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• 2013

Objectives : The purpose of this study was to investigate the protective effects of Magnolia Officinalis extracts on the animal model of depression induced by immobilization stress. Methods : The subjects were divided into 4 groups : normal, saline solution-administered during immobilization stress, 200 mg/kg of magnolia extracts-administered (magnolia extract 200), and 400 mg/kg of magnolia extracts- administered (magnolia extract 400). During 2 days of immobilization stress treatment, they underwent forced swimming test (FST) and tail suspension test (TST). The number of serotonin (5-HT) immunostained nuclei in the dorsal raphe nucleus regions was measured by immunohistochemistry. Superoxide dismutase (SOD) and glutathione peroxidase (GPX) in blood were measured. Results : In FST, magnolia-administered groups showed significantly decreased immobilization. In TST, the magnolia extract 400 group showed decreased immobilization. The stress group showed significantly decreased number of 5-HT immunostained nuclei in the dorsal raphe nucleus regions, while magnolia extract 400 group showed increased number of 5-HT immunostained nuclei. Stress group showed decrease in serum level of SOD and GPX, while the magnolia extract 200 group showed increase in serum level of SOD and GPX. Conclusions : These results suggest potent effectiveness of magnolia extracts in the treatment of depression.

• Journal of the Korean Data and Information Science Society
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• 제17권4호
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• pp.1375-1386
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• 2006

In life testing, the lifetimes of test units under the usual conditions are so long that life testing at usual conditions is impractical. Testing units are subjected to conditions of high stress to yield informations quickly. In this paper, the inferences of parameters on the three step-stress accelerated life testing are studied. The two-parameter exponential distribution with a failure rate function that a log-quadratic function of stress and the tempered failure rate model are considered. We obtain the maximum likelihood estimators of the model parameters and their confidence regions. A numerical example will be given to illustrate the proposed inferential procedures.

• 대한의용생체공학회:의공학회지
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• 제6권2호
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• pp.9-12
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• 1985

The wall shear stress in the vicinity of end-to end anastomoses under steady flow conditions was measured using a flush-mounted hot-film anemometer(FMHFA) probe. The experimental measurements were in good agreement with numerical results except in flow with low Reynolds numbers. The wall shear stress increased proximal to the anastomosis in flow from the Penrose tubing (simulating an artery) to the PTFE: graft. In flow from the PTFE graft to the Penrose tubing, low wall shear stress was observed distal to the anastomosis. Abnormal distributions of wall shear stress in the vicinity of the anastomosis, resulting from the compliance mismatch between the graft and the host artery, might be an important factor of ANFH formation and the graft failure. The present study suggests a correlation between regions of the low wall shear stress and the development of anastomotic neointimal fibrous hyperplasia(ANPH) in end-to-end anastomoses.

• Structural Engineering and Mechanics
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• 제18권5호
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• pp.541-563
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• 2004

For reaching large inelastic deformations without a substantial loss in strength, the potential plastic hinge regions of the reinforced concrete structural members should be confined by adequate transverse reinforcement. Therefore, simple and realistic representation of confined concrete behaviour is needed for inelastic analysis of reinforced concrete structures. In this study, a trilinear stress-strain model is proposed for the axial behaviour of confined concrete. The model is based on experimental work that was carried out on nearly full size specimens. During the interpretation of experimental data, the buckling and strain hardening of the longitudinal reinforcement are also taken into account. The proposed model is used for predicting the stress-strain relationships of confined concrete specimens tested by other researchers. Although the proposed model is simpler than most of the available models, the comparisons between the predicted results and experimental data indicate that it can represent the stress-strain relationship of confined concrete quite realistically.