• Proceedings of the Microbiological Society of Korea Conference
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• 2007.05a
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• pp.120-122
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• 2007

All living organisms use numerous signal-transduction pathways to sense and respond to their environments and thereby survive and proliferate in a range of biological niches. Molecular dissection of these signalling networks has increased our understanding of these communication processes and provides a platform for therapeutic intervention when these pathways malfunction in disease states, including infection. Owing to the expanding availability of sequenced genomes, a wealth of genetic and molecular tools and the conservation of signalling networks, members of the fungal kingdom serve as excellent model systems for more complex, multicellular organisms. Here, we employed Cryptococcus neoformans as a model system to understand how fungal-signalling circuits operate at the molecular level to sense and respond to a plethora of environmental stresses, including osmoticshock, UV, high temperature, oxidative stress and toxic drugs/metabolites. The stress-activated p38/Hog1 MAPK pathway is structurally conserved in many organisms as diverse as yeast and mammals, but its regulation is uniquely specialized in a majority of clinical Cryptococcus neoformans serotype A and D strains to control differentiation and virulence factor regulation. C. neoformans Hog1 MAPK is controlled by Pbs2 MAPK kinase (MAPKK). The Pbs2-Hog1 MAPK cascade is controlled by the fungal "two-component" system that is composed of a response regulator, Ssk1, and multiple sensor kinases, including two-component.like (Tco) 1 and Tco2. Tco1 and Tco2 play shared and distinct roles in stress responses and drug sensitivity through the Hog1 MAPK system. Furthermore, each sensor kinase mediates unique cellular functions for virulence and morphological differentiation. We also identified and characterized the Ssk2 MAPKKK upstream of the MAPKK Pbs2 and the MAPK Hog1 in C. neoformans. The SSK2 gene was identified as a potential component responsible for differential Hog1 regulation between the serotype D sibling f1 strains B3501 and B3502 through comparative analysis of their meiotic map with the meiotic segregation of Hog1-dependent sensitivity to the fungicide fludioxonil. Ssk2 is the only polymorphic component in the Hog1 MAPK module, including two coding sequence changes between the SSK2 alleles in B3501 and B3502 strains. To further support this finding, the SSK2 allele exchange completely swapped Hog1-related phenotypes between B3501 and B3502 strains. In the serotype A strain H99, disruption of the SSK2 gene dramatically enhanced capsule biosynthesis and mating efficiency, similar to pbs2 and hog1 mutations. Furthermore, ssk2, pbs2, and hog1 mutants are all hypersensitive to a variety of stresses and completely resistant to fludioxonil. Taken together, these findings indicate that Ssk2 is the critical interface protein connecting the two-component system and the Pbs2-Hog1 pathway in C. neoformans.

• Current Optics and Photonics
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• v.3 no.6
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• pp.502-509
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• 2019

Based on the typical pixel structure and parameters of a polysilicon uncooled bolometer, the absorption rate of a polysilicon microbridge infrared detector for 10.6 ㎛ laser energy was calculated through the optical admittance method, and the thermal coupling model of a polysilicon microbridge component irradiated by far infrared laser was established based on theoretical formulas. Then a numerical simulation study was carried out by means of finite element analysis for the actual working environment. It was found that the maximum temperature and maximum stress of the microbridge component are approximately exponentially changing with the laser power of the irradiation respectively and that they increase monotonically. The highest temperature zone of the model is gradually spread by the two corners of the bridge surface that are not connected to the bridge legs, and the maximum stress acts on both sides of the junction of the microbridge legs and the substrate. The mechanism of laser-induced hard damage to polysilicon detectors is the melting damage caused by high temperature. This paper lays the foundation for the subsequent study of the interference mechanism of the laser on working state polysilicon detectors.

• Journal of Korean Society of Industrial and Systems Engineering
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• v.41 no.4
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• pp.213-219
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• 2018

Mechanical components are to be produced with accurate dimensions in order to function properly in assemblies of a machine. Once designs of mechanical components are created, designers examine the designs by adopting many known experimental methods. A primary test method includes stress and strain evaluation of structural parts. In addition, fatigue test and vibration analysis are an important test method for mechanical components. Real experiments at a laboratory are established when products are manufactured. Since design changes should be done before producing the designs in factories, rapid modifications for new designs are required in production industries. FEM simulation is a proper choice for a design evaluation with speed at a detail stage in design process. This research focuses modeling and mechanical simulation of a mechanical component in order to ensure structural safety. In this paper, a universal joint, being used in driving axels of vehicles, is studied as a target component. A design model is created and tested in some ways by using commercial software of FEM. The designed component is being twisted to transmit heavy power and thus, torsional stress should be under strengths of the component's material. The next is fatigue analysis to convince fatigue cycles to be within the endurance limit of the material. Another test is a vibration analysis for rotational components. This research draws final conclusions from these test analyses and recommends whether the designed model is under safety condition in terms of mechanical structure.

• Korean Journal of Health Education and Promotion
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• v.32 no.3
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• pp.73-83
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• 2015

Objectives: The purpose of this study was to identify the effects of sleep quality and occupational stress on health-related quality of life among a university's security workers. Methods: 128 security workers voluntarily participated in the study. The survey asked demographic, health related, job related factors, the Korean Occupational Stress Scale Short Form(KOSS-SF), sleep quality questionnaire, and 12-Short Form Health Survey version 2.0(SF-12v2). The data were analyzed by using descriptive analysis, t-test, ANOVA, multiple linear regression, and multiple logistic regression. Results: The interrupted rest time was significantly negatively related to occupational stress. The occupational stress significantly affected sleep quality. The significant variable affecting Physical Component Score(PCS) and Mental Component Score(MCS) was sleep quality and occupational stress, respectively. Conclusions: HRQOL was founded to be affected by occupational stress and sleep disorder among the security workers. Based on this study, we suggest that the management of both occupational stress and sleep quality is crucial to improve the HRQOL.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2006.11a
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• pp.118-119
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• 2006

현재의 소자간 연결을 위해 사용되는 금속배선의 한계로 인해 보다 고속/대용량의 광연결(Optical Interconnection)이 크게 각광받고 있다. 본 논문에서는 FEM 시뮬레이션(Finite Element Method Simulation)을 통해 온도변화에 따른 기판에서의 온도분포를 살펴보고, 열응력 분포와 열응력 집중에 의한 기판의 변형으로 인한 문제를 연구하였다. 이를 통해 향후 Optical Passive Component 설계시 Optical Passive Component 변형의 원인이 될 수 있는 열원들의 배치를 최적화 시키고 기판의 취약부운을 보강하여 우수한 성능의 Optical Passive Component 제작을 목표로 하고 있다.

• The Transactions of the Korea Information Processing Society
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• v.7 no.3
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• pp.842-851
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• 2000

Developers can reuse not only class code but also wide range of knowledge on domain by reusing framework. Existing Object-Oriented Methodology and Catalysis Methodology were presented when redefining component in the course of redesigning framework. However, existing methodologies have weakness that entire process is waterfall mode or design of interface lays too much stress on implementation stage. So, this thesis will present Component-Oriented Methodology for the reuse of framework, and construct the environment for framework and domain development. That is, domain is analyzed by input of domain knowledge on real world to create software based on component, and hotspot is identified through analyzed information, and refactoring by putting additional information on users and developers. After that, I will create domain framework and application framework depending on domain. In this Component-Oriented Methodology, information is searched, understood and extracted or composite through component library storage internally. Then this information is classified into the information on component, and used as additional information in redesigning. With this, developer can obtain reusability, easiness and portability by constructing infrastructure environment that allows to register, update and delete component through Component Management System(CMS) under he development environment which can be easily applied to his own application using framework component, in this thesis, CoRBA(Common Object Request Broker Architecture) environment.

• Journal of the Korean Geotechnical Society
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• v.31 no.11
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• pp.15-23
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• 2015

An experimental study has been conducted to evaluate the effects of average and cyclic shear stresses on the undrained failure behaviors of dense marine silty sand by using the Cyclic Direct Simple Shear apparatus. The results show that when the average shear stress ratio is zero, symmetric cyclic shear deformation is the major component of deformation, and permanent shear deformation is relatively small. On the other hand, when the average shear stress ratio is larger than zero, asymmetric permanent shear deformation is the major component, and cyclic shear deformation does not change much as the number of cyclic loads increases. The average shear stress ratio has less effects on the number of cyclic loads needed to fail, as compared with the cyclic shear stress ratio. The proposed stress-dependent failure contour can effectively be used to assess the cyclic shear strength of soil beneath the foundation for the design of offshore structures.

• Journal of Dental Rehabilitation and Applied Science
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• v.17 no.4
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• pp.307-314
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• 2001

To evaluate the effect of misfit in two implant-supported fixed partial dentures in the posterior of the mandible, variations of the standard finite element models were made by changing the location of the gap as follows: 1) no gap present; 2) located between the gold cylinder and the abutment on the distal implant; 3) gap located between the gold cylinder and the abutment on the mesial implant. The results of this study were as follows: 1. When the location of the gap was close to the load applied on the prosthesis, the stress in the prosthesis, implant components and surrounding bone increased. 2. The presence of cantilever increased the stress in the prosthesis, implant and surrounding bone significantly, regardless of the presence of the gap. 3. When there was a gap between the prosthesis and abutment, the stress in the bone around the implant increased. 4. When passive fit was achieved, the stress was distributed widely in each component with less peak stress in each component. 5. The inner structures of the implant components, the gold screw and the abutment screw bear more stress when the prosthesis did not exhibit passive fit with the abutments than when passive fit was present.

• The korean journal of orthodontics
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• v.41 no.6
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• pp.423-430
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• 2011

Objective: To evaluate the extent and aspect of stress to the cortical bone after application of a lateral force to a two-component orthodontic mini-implant (OMI, mini-implant) by using three-dimensional finite element analysis (FEA). Methods: The 3D-finite element models consisted of the maxilla, maxillary first molars, second premolars, and OMIs. The screw part of the OMI had a diameter of 1.8 mm and length of 8.5 mm and was placed between the roots of the upper second premolar and the first molar. The cortical bone thickness was set to 1 mm. The head part of the OMI was available in 3 sizes: 1 mm, 2 mm, and 3 mm. After a 2 N lateral force was applied to the center of the head part, the stress distribution and magnitude were analyzed using FEA. Results: When the head part of the OMI was friction fitted (tapped into place) into the inserted screw part, the stress was uniformly distributed over the surface where the head part was inserted. The extent of the minimum principal stress suggested that the length of the head part was proportionate with the amount of stress to the cortical bone; the stress varied between 10.84 and 15.33 MPa. Conclusions: These results suggest that the stress level at the cortical bone around the OMI does not have a detrimental influence on physiologic bone remodeling.

• Journal of Biomedical Engineering Research
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• v.20 no.1
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• pp.37-44
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• 1999

Because of bone resorption, wear of ultra-high molecular weight polyethylene(UHMWPE) in total knee arthroplasty has been recognized as a major factor in long-term failure of knee implant. The surface damage and the following harmful wear debris of UHMWPE is largely related to contact stress. Most of the previous studies focused on the contact condition only at the articulating surface of UHMWPE. Recently, contact stress at the metal-backing interface has been implicated as one of major factors in UHMWPE wear. Therefore, the purpose of the is study is to investigate the effect of the contact stress for different thickness, conformity friction coefficient, and flexion degree of the UHMWPE component in total knee system, considering the contact conditions at both interfaces. In this study, a two-dimensional non-linear plane strain finite element model was developed. The results showed that the maximum value of von-Mises stress occurred below the articulating surface and the contact stress was lower for the more conforming models. All-polyethylene component showed lower stress distribution than the metal-backed component. With increased friction coefficient on the tibiofemoral contact surface, the maximum shear stress increased about twofold.