• Biomolecules & Therapeutics
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• 제2권3호
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• pp.223-228
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• 1994

In this study, we examined gangliosides from streptozotocin-induced diabetic rat brain. To obtain the diabetic rat brain, we sacrified the rat three days after injecting the streptozotocin into venus in tail. We measured blood glucose level according to Somogy-Nelson method and measured insulin level using $^{125}$ I-insulin RIA kit. The gangliosides were extracted according to Folch-Suzuki method from the rat brain. We also examined the effect of major lipid components extracted from deer antler on diabetic rat brain. The results showed that the major lipids components lowered both blood glucose and insulin level in normal rat. However only the blood glucose level in diabetic rat was lowered with major lipid components. In diabetic rat brain, gangliosides metabolism were changed. The amount of GMla was increased while GDla, GDlb, and GTlb were not synthesized. Furthermore, undefined ganglioside was found. In major lipid component-treated diabetic rat brain, the ganglioside metabolism proceeded as same as the normal rat. On the contrary, in bovine brain gangliosides-treated diabetic rat brain, the gangliosides metabolism was not recovered to normal one.

• 생명과학회지
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• 제14권2호
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• pp.245-251
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• 2004

본 연구는 streptozotocin으로 당뇨병을 유도하는 조건들을 농도에 따른 변화를 관찰하고, 적절한 조건에서 당뇨쥐에서의 저강도의 treadmill 운동이 체중과 혈중의 당, 인슐린 및 지질의 변화에 미치는 영향을 알아보고자 실시하였으며, 연구결과를 요약하면 다음과 같다. 실험 I에서 STZ 주사량이 40 mg/mg으로 높아질수록 정상군에 비해 혈당이 유의하게 증가하고, 반대로 체중과 인슐린 농도는 상대적으로 감소하였다. 실험II에서 STZ 주사를 한 모든 군에서 혈당이 유의하게 증가하지만 BE군은 C군에 비해 그 증가율이 유의하게 억제되었으며, 운동이 인슐린 농도와 혈중지질 농도를 정상적으로 유도하는 것으로 나타났다. 특히, 당뇨 유발 전부터 운동을 실시한 경우 그 효과가 더욱 유의하게 나타났으며, 앞으로 당뇨 유발의 정도와 운동 빈도 및 강도와 관련된 더욱 많은 연구가 이루어져야 할 것으로 사료된다.

• BMB Reports
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• 제41권10호
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• pp.710-715
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• 2008

This study investigated the effect of Phellodendri Cortex extract on hyperglycemia and diabetic nephropathy in streptozotocin-induced diabetic rats. Male Sprague-Dawley rats were divided into normal control (NC), diabetic control (DC), and diabetic treatment with Phellodendri Cortex extract (DP). Over a 4-week experimental period, Phellodendri Cortex extract was administered orally at 379 mg/kg BW/day. The final fasting serum glucose level, urine total protein level, and relative left kidney weight in the DP group were significantly lower than the DC group. Renal XO and SOD activities in the DP group were significantly lower than the DC group and renal CAT activity in the DP group was significantly higher than the DC group. Tubular epithelial change was reduced in the DP group compared to the DC group. These results indicated that Phellodendri Cortex can reduce glucose level and prevent or retard the development of diabetic nephropathy in streptozotocin-induced diabetic rats.

• 한국식품영양과학회지
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• 제24권2호
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• pp.336-340
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• 1995

뇨중에 배설되는 아미노산으로 당뇨병 rat의 채단백질 대사상태와 식이단백질의 이용효율을 검토하였다. Controi group의 무단백질 식이 rat 뇨 증 아미노산 배설량과 당뇨병 유발 rat의 배설량과 비교한 결과, 당뇨병 group에서 아미노산이 많이 배설되었지만, 각 아미노산의 조성은 거의 같은 pattern을 보여 주고 있으므로 뇨 중 아미노산이 내인성 대사 생성물임이 확인되었다. 그리고 뇨 중 아미노산을 토대로 당뇨병 rat 식이단백질의 이용효율은 control 보다 낮았다.

• 대한한방내과학회지
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• 제35권4호
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• pp.519-531
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• 2014

Objectives: Diabetic nephropathy is the most common cause of end stage renal disease. Transforming growth factor (TGF)-${\beta}1$, type IV collagen, advanced glycation end-products (AGEs), and angiotensin II type 1 receptor (AT1) are the main factors of diabetic nephropathy. We investigated the effects of Prunus on renal function and histopathological changes of diabetic nephropathy rat model induced by unilateral nephrectomy and streptozotocin. Methods: Diabetes was induced in male Sprague-Dawley rats ($290{\pm}10g$) by injecting streptozotocin (55 mg/kg) into the tail vein after unilateral nephrectomy. Rats were divided into 3 groups (n=6): normal, control, and Prunus. After 8 weeks of oral administration of Prunus extract on the Prunus group from 3 days after streptozotocin injection, we checked weight, 24 hrs urine, blood biochemistry and renal tissue to evaluate renal function and histopathological changes by examining parameters including albuminuria, BUN, creatinine, cholesterol, low density lipoprotein (LDL), triglyceride, TGF-${\beta}1$, type IV collagen, AGEs, and AT1. We also measured mRNA expression of TGF-${\beta}1$, type IV collagen, AGEs, and AT1 by Real Time polymerase chain reaction (RT-PCR). Results: Prunus decreased the amount of 24 hrs proteinuria, and inhibited histopathological changes of diabetic nephropathy including the expression and accumulation of TGF-${\beta}1$, type IV collagen and AGEs which could promote development of diabetic nephropathy. Prunus also inhibited mRNA expression of TGF-${\beta}1$, type IV collagen. Conclusions: These findings suggest that Prunus might protect the renal function and inhibit the development of renal injury by regulating factors including TGF-${\beta}1$, type IV collagen, AGEs, except AT1, so Prunus can be used for diabetic patients to prevent the progression of diabetic nephropathy.

• 대한본초학회지
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• 제21권3호
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• pp.75-81
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• 2006

Objectives : The present study was carried out to investigate the beneficial effect of Coicis Semen extract(CSe) on streptozotocin(STZ)-induced diabetic nephropathy rats. CSe was given to rats with oral administration. Methods : The experimental animals were divided into 3 groups : normal group of rats, control group of STZ-induced diabetic rats, sample group with CSe treatment. Experimental diabetes was induced by the injection of STZ(60 mg/kg) to the rat via the peritoneum. The effects of CSe on STZ-induced diabetic nephropathy were observed by measuring the serum level of creatinine, BUN and uric level of glucose. Kidney level of lipid peroxidation and the activities of reduced glutathione(GSH) were also examined Results : STZ-induced increase of serum creatinine was lowered by CSe treatment, but BUN and uric level of glucose did not show significant changes. CSe oral administration showed statistical decrease of lipid peroxidation in renal cortical tissues, but it has no effect on the activities of GSH. Conclusion : CSe treatment showed protective effect on rat diabetic nephropathy model, but action mechanism of the effect was still not dear. We thought to be concerned with anti-oxidative stress.

• Applied Microscopy
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• 제23권2호
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• pp.84-96
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• 1993

This study was to investigate the effects of maternal diabetes on the lung tissue of the fetal rat using lectin histochemistry and electron microscope technique. Maternal diabetes was induced by intraperitoneal injection of streptozotocin (75 mg/kg the body weight) into pregnant Sprague-Dawley rats on the 7th day of gestation. Fetuses of streptozotocin induced diabetic rats exhibited delayed lung maturation and reduced air space. In lectin histochemistry, the binding of Maclura pomifera (MPA) to fetal lungs from diabetic mothers was reduced, but no significant changes in the bindings of Concanavalin A (Con A), Wheat germ agglutinin (WGA), Ricinus communis I (RCA I) and Griffonia simplicifolia (GSI-$B_4$) were noted. Because the MPA has affinity to terminal N-acetyl-D-galactosamine residues constantly linked O-glycosidically to serine or threonine, the present findings may indicates that maternal diabetes interfere with the processes of O-linked glycosylation in fetal rat lung.

• Journal of Nutrition and Health
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• 제27권8호
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• pp.819-827
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• 1994

The present study was undertaken to evaluate the effects of 50% buckwheat diet on the body weight, organ weight, urine albumin, urine glucose, plasma glucose and plasma lipid in normal rats and diabetic rats treated with streptozotocin(STZ). The food intake, body weight, the level of urine glucose in diabetic buckwheat groups were not significantly different with diabetic control group. The level of urine albumin was lower in raw and steam buckwheat group than in the diabetic control group. Compared to the normal control group, liver and kidney weights were heavier in the diabetic groups. Pancreas weight was heavier diabetic buckwheat groups than in normal and diabetic control groups. Fasting plasma glucose level of diabetic buckwheat groups significantly decreased by 18-37% compared with the diabetic control group. Plasma triglyceride level of diabetic buckwheat groups significantly decreased by 34-50% compared with the diabetic control group. Plasma total cholesterol level of diabetic buckwheat groups decreased by 15-27% compared with the diabetic control group. The level of HDL-cholesterol was not affected by buckwheat diet. These results indicate that buckwheat is an effective therapeutic regimen for the control of metabolic derangements in diabetics.

• 한국임상수의학회지
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• 제36권3호
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• pp.145-149
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• 2019

Previous report has suggested that the albumin levels were reduced in the peripheral blood mononuclear cells (PBMCs) and consequently oxidative stress was elevated in streptozotocin (STZ)-induced diabetic rats as albumin is the predominant antioxidant in plasma. In this study, we suggest that the levels of pro-inflammatory cytokine such as interleukin-6 (IL-6) and tumor necrosis factor-${\alpha}$ ($TNF-{\alpha}$) were increased by approximately 3.4- and 2.9-fold, respectively, in the serum of STZ-diabetic rats, compared to those of normal rat. In addition to the cytokines, the levels of C-reactive protein (CRP) were also about 3.6-fold higher, indicating that STZ induced a pro-inflammatory response in rat blood. However, when purified rat albumin was externally co-administrated with STZ through the tail vein, the serum levels of IL-6, $TNF-{\alpha}$, and CRP were markedly reduced, although the values were still higher than those of normal (non-diabetic) rats. Albumin administration also decreased STZ-induced oxidative stress in serum and PBMCs. Moreover, the decrease in cytokine and CRP levels was dependent on the dose of injected albumin. These results suggest that STZ-induced pro-inflammation and oxidative stress in rat blood might be attenuated by treatment with exogenous albumin.

• 대한한의학회지
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• 제27권2호
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• pp.155-170
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• 2006

Objectives : This study aimed to evaluate the hypoglycemic, hepatoprotective, and nephroprotective effects of 'Saengjinyanghyul-tang($Sh\={e}ngj\={i}ny\v{a}ngxu\`{e}-t\={a}ng$ : SJYHT)' in streptozotocin (STZ)-induced diabetic SD rat model. Methods : SJYHT extracts were once a day dosed for 28 days at a dosage 1000, 500, and 250mg/kg/5ml from 25 days after STZ-dosing, and the changes on blood glucose levels, liver and kidney weight, serum AST, ALT, BUN and creatinine levels were observed with histological changes on the liver and kidney. Results : Increased blood glucose levels, and diabetic hepatopathy & nephropathy including increased serum AST, ALT, BUN and creatinine levels, histological changes after STZ-dosing were dose-dependently reduced by SJYHT extract-dosing. Conclusions : 'SJYHT' water extracts have favorable hypoglycemic, hepatoprotective, and nephroprotective effects on the STZ-induced diabetic SD rats. Therefore, it is expected that 'SJYHT' has potential for use in the management of diabetes and diabetic complications.