• Imaging Science in Dentistry
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• 제35권3호
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• pp.147-156
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• 2005

Purpose : To observe the histopathological changes and caspase-3 expression in the submandibular gland in streptozotocin-induced diabetic rats after irradiation. Materials and Methods : The male Sprague-Dawley rats weighing approximately 250 gm were divided into four groups: control, diabetes, irradiation, and diabetes-irradiation groups. Diabetes mellitus was induced in the rats by injecting streptozotocin. Rats in the control and irradiation groups were injected with citrate buffer only. After 5days, rats in irradiation and diabetes-irradiation groups were irradiated with a single absorbed dose of 10 Gy to the head and neck region. All the rats were sacrificed at 3, 7, 14, 21, and 28 days after irradiation. The specimen including the submandibular gland were sectioned and observed using histopathological and immunohistochemical methods. Results : In the irradiation group, the condensed nucleus, karyolysis, and degeneration of the acinar cells and atrophy of the duct cells were observed in the early experimental phase. However, the acinar cells were found to be normal at 28 days after irradiation. In the diabetes group, the condensed nucleus, karyolysis, atrophy, and degeneration of the acinar cells were observed in the early experimental phase. However, the acinar cells were found to be normal at 21 days after diabetic state induction. In the diabetes-irradiation group, the ductal epithelial cells were predominant in their glandular tissues at 28 days after irradiation. In all of the experimental groups, the most prominent change of the acinar cells and ductal cells were observed at 14 days after diabetic state induction and irradiation. Conclusion The expression of caspase-3 in the acinar cells and ductal cells of the submandibular gland was weak after irradiation, but that in the acinar cells, ductal cells, and fibrous cells of the submandibular gland was prominent after diabetic state induction.

• Biomolecules & Therapeutics
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• 제26권2호
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• pp.130-138
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• 2018

Quercetin and resveratrol are known to have beneficial effects on the diabetes and diabetic complication, however, the effects of combined treatment of these compounds on diabetes are not fully revealed. Therefore, the present study was designed to investigate the combined antidiabetic action of quercetin (QE) and resveratrol (RS) in streptozotocin (STZ)-induced diabetic rats. To test the effects of co-treated with these compounds on diabetes, serum glucose, insulin, lipid profiles, oxidative stress biomarkers, and ions were determined. Additionally, the activities of hepatic glucose metabolic enzymes and histological analyses of pancreatic tissues were evaluated. 50 male Sprague-Dawley rats were divided into five groups; normal control, 50 mg/kg STZ-induced diabetic, and three (30 mg/kg QE, 10 mg/kg RS, and combined) compound-treated diabetic groups. The elevated serum blood glucose levels, insulin levels, and dyslipidemia in diabetic rats were significantly improved by QE, RS, and combined treatments. Oxidative stress and tissue injury biomarkers were dramatically inhibited by these compounds. They also shown to improve the hematological parameters which were shown to the hyperlactatemia and ketoacidosis as main causes of diabetic complications. The compounds treatment maintained the activities of hepatic glucose metabolic enzymes and structure of pancreatic ${\beta}-cells$ from the diabetes, and it is noteworthy that cotreatment with QE and RS showed the most preventive effect on the diabetic rats. Therefore, our study suggests that cotreatment with QE and RS has beneficial effects against diabetes. We further suggest that cotreatment with QE and RS has the potential for use as an alternative therapeutic strategy for diabetes.

• Food Science and Biotechnology
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• 제17권2호
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• pp.287-294
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• 2008

Protective effects of cheonggukjang fermented with 20% red ginseng (RC) were observed in streptozotocin (STZ)-induced diabetic rats by measuring levels of blood glucose, serum lipid profiles, and hepatic reactive oxygen species generating and scavenging enzymc activities. RC diet was prepared by mixing with AIN-76 diet at the final concentration 2%, and it was fed to STZ-induced diabetic rats for 3 weeks. The RC dict was significantly improved body weight, feed efficiency ratio, levels of serum glucose, and serum and hepatic lipids in diabetes. The significantly elevated O type activity of xanthine oxidase in diabetes was also greatly decreased by the RC diet. The treatment of RC showed the improved hepatic glutathione s-transferase activities in the diabetic animals. The present study indicates that cheonggukjang fermented with red ginseng could ameliorate STZ-induccd diabetic symptoms such as aggravated blood glucose levels, serum lipid profiles, and even the conditions of oxidative stress.

• 한국식품위생안전성학회지
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• 제16권4호
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• pp.349-354
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• 2001

산화적 스트레스는 당뇨병과 관련된 맥관계병변의 전개에 아주 중요한 역할을 한다고 알려져 있다. 따라서 본 실험에서는 streptozotocin(STZ)투여에 의한 당뇨유발쥐를 사용하여 참께함유 항산화성분인 sesamin과 sesamolin의 혈당과 생체내 항산화 방어효소에 미치는 효과에 대하여 알아보고자, 당뇨유발쥐에 0.2% sesamin또는 0.2% sesamolin 함유사료를 3주간 급여하여, 혈당농도, 뇨당농도, 혈청 GOT와 GPT활성, 혈청 및 간조직의 지질과산화물,그리고 GSH함량과 GST활성에 미치는 영향을 조사하였다. STZ투여군의 혈당량은 319.4 $\pm$105.74mg/dL였는데, sesamin급여군은 146.56$\pm$23.99mg/dL, sesamolin급여군은170.74$\pm$105.94mg/dL로 유의하게 감소하였다. 당뇨병쥐의 뇨당농도가 21.16$\pm$13.72 mg/dL였는데, 세사민과 세사몰린 급여시에는 19.52 $\pm$ 8.99 mg/day와 10.93 $\pm$ 7.79 mg/day로 유의하게 감소하였다. STZ투여에 의하여 증가된 GOT와 GPT활성도는 세사민과 세사몰린의 급여에 의하여 GPT활성도만이 유의하게 감소되었다. STZ투여에 의해 증가한 간조직 중의 지질과산화물 생성은 세사민과 세사몰린 급여에 의해 유의하게 감소하였다. STZ투여에 의해 감소된 신장의 GSH함량과 GST활성은 세사민과 세사몰린에 의해 회복되었다. 이상의 결과 세사민과 세사몰린이 신장조직에서 일차적으로 산화된 산화물을 포집하고 GSH를 이용하여 체내의 독성 물질을 전이 분해시키는 작용을 하여 STZ에 의해 유도된 각종 독성을 무독화시켜 신장의 손상을 보호하여 당뇨억제 작용과 함께 항산화 효과를 나타낸 것으로 생각된다.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1993년도 제2회 신약개발 연구발표회 초록집
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• pp.175-175
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• 1993

Streptozotocin, which is a naturally occurring nitrosoamide used extensively to produce diabetes mellitus in experimental animals, has been known to be chemically stable only under acidic condition (pH 4). The present study was conducted to determine whether its degradation products formed under various conditions can induce hyperglycemia in female rats. Streptozotocin in phosphate buffer saline (pH 7.4) resulted in spontaneous degradation rapidly. Furthermore, streptozotocin incubated with plasma isolated from rats was degraded even faster than those in neutral buffer. When streptozotocin was administered iv to rats, significant elevations in blood glucose level were observed within 24 hours. However, rats treated with equimolar concentration of streptozotocin degradation products under the phosphate buffer saline as well as the plasma did not lead to hyperglycemia. These results suggest that, when streptozotocin administered undergo spontaneous breakdown in vivo, its degradation Products do not induce the hyperglycemia in rats.

• 생명과학회지
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• 제19권3호
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• pp.334-342
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• 2009

본 연구는 streptozotocin (STZ)으로 당뇨를 유발한 실험쥐에 대한 Psidium guajava L. 잎(Pg), Lagerstroemia speciosa L. 잎(Ls) 추출물 그리고 혼합물 A (Pg, Ls, Morus indica L. 잎 추출물, Pinus densiflora needles 추출물, Acanthopanax senticosus M. roots 추출물)의 효과를 조사하였다. 4주간 streptozotocin (STZ)으로 당뇨를 유발한 실험쥐에 이들 추출물을 섭취시킨 결과 Pg, Ls 그리고 혼합물 A의 섭취는 당뇨 대조군에 비해 혈당을 각각 20%, 14% 그리고 24% 감소시켰다. 또한 이들 추출물의 섭취는 총 콜레스트롤, 중성지방, 유리지방산을 감소시켰고 인슐린과 HDL-콜레스트롤을 효과적으로 증가시켰다. 결론적으로 혼합물 A는 Pg와 Ls 추출물에 비해 혈당 및 지질 감소, 인슐린 증가 효과 그리고 기능적으로 손상된 베타세포의 회복이 더 높은 것으로 나타났다. 따라서 혼합물 A는 개별적으로 사용한 Pg와 Ls 추출물에 비해 더욱 유익한 상승효과를 발휘하는 것으로 증명되었다.

• 대한한의학방제학회지
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• 제14권2호
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• pp.57-66
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• 2006

Objectives : The objective of this study is to observe the effect of Saryuktangkamibang, Saryuktang with Liriopis Tuber, Lycii Fructus, Schizandrae Fructus, and Bombyx Batryticatus, on the experimental diabetes. Methods : In order to induce diabetes experimentally, injected streptozotocin to the vein in the tail of rats and then treated oral administration of Saryuktangkamibang water extracts. In the measurement of the variation levels of glucose, ALP, creatinine, and BUN concentration for each concentration levels for serum (0.2g/100g, 0.4g/l00g, 0.6g/100g), concentration level of glucose significantly decreased in 0.4g/l00g concentration level of Saryuktangkamibang. With this 0.4g/l00g concentration level of Saryuktangkamibang, the following conclusion was derived from the measurement of the serum levels of glucose. ALP, AST, ALT, creatinine, and BUN concentration for the injection of each 4th, 11th, and 18th day. Results : 1. In the measurement for each concentrations, the glucose concentration level in the serum significantly decreased on the 9th day in the Saryuktangkamibang-dosed group. 2. The creatinine and BUN concentration level in the serum significantly decreased on the 9th day in the Saryuktangkamibang-dosed group to which 0.6g/100g injected. 3. In the measurement for each dosage dates, the glucose concentration level in the serum significantly decreased on the 11th day in the Saryuktangkamibang-dosed group to which 0.6g/100g injected. 4. The AST concentration level in the serum significantly decreased on the 11th day in the Saryuktangkamibang-dosed group. Conclusion : Saryuktangkamibang, Saruktang with Liriopis Tuber, Lycii Fructus, Schizandrae Fructus, and Bombyx Batryticatus, has an effect of hypoglycemia, with no hepato-renal dysfunction. For the experimental results above, in that nephrotoxicity significantly decreased on the 0.6g/100g concentration, another research such as study for effect in other ranges and study for effect of dosage term except the concentration range of this study is necessary. Also dosage of appropriate concentration of Saryuktangkamibang is thought to provide valuable aid to cure on diabetes.

• Journal of Nutrition and Health
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• 제27권9호
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• pp.883-891
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• 1994

The number of diabetics in Korea is about 3 to 5 percent of the population, and the incidence is increasing yearly due to changes of life style and food intake. Diet is a key element in the management of diabetes, yet the appropriate diet for diabetes remains controversial. We have recently shown that a diet rich in protein of animal origin(casein) seems beneficial to controling plasma glucose and lipids in streptozotocin-induced diabetic rats. It therefore seemed desirable to find out whether the beneficial effect of high casein diet in experimental diabetes could also be reproduced with a vegetable source of protein(soy). The purpose of this study is to compare these results with the results of our previous study. In the present study, non-diabetic and streptozotocin-induced diabetic rats were studied in order to examine the effects of altering the level(20% vs 60%) of dietary soy protein on blood glucose, lipids, and hormones. Results of the present study showed that a high soy protein diet decreased triglyceride concentration in diabetic rats. However, diabetic rats fed a high soy protein diet were not hypocholesterolemic compared to rats fed a control diet. Moreover, diabetic rats fed a high soy protein diet had significantly increased plasma glucose concentration compared to rats fed a control diet. This study was not able to discern a specific effect of dietary protein level on insulin, glucagon, or insulin/glucagon ratio. Except for the hypotriglyceridemic effect, the results were not similar to the findings of our previous study which showed a beneficial effect on streptozotocin-induced diabetic rats fed a high casein diet.

• 대한약리학회지
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• 제27권1호
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• pp.1-5
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• 1991

Streptozotocin(STZ)-유발 당뇨쥐에서 시상하부의 norepinephrine(NE) 대사를 기초 NE 함량, NE 교체율, in vivo tyrosine hydroxylase(TH) 활성을 그 지표로 하여 조사하였다. STZ (60 mg/kg, iv)로 당뇨를 유발한 후 4주까지 기초 NE 함량은 유의한 변화가 없었다. 그러나 당뇨유발 후 1주째에 측정한 NE 교체율은 대조치의 62%(p<0.01), in vivo TH 활성은 대조치의 34% (p<0.05)로 감소하였다. 이상의 결과로 본 실험에서 측정한 NE 대사의 세 지표 중에서 in vivo TH 활성이 STZ-유발 당뇨쥐의 시상하부 NE 대사의 변화를 가장 민감하게 반영하였다.

• 운동과학
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• 제21권3호
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• pp.289-298
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• 2012

본 연구는 당뇨를 유발한 흰쥐에서 트레드밀 운동이 망막 신경세포의 사멸에 억제 효과가 있는지를 실험 하였다. 본 연구에서 Sprague-Dawley계 흰쥐 28마리를 대조군, 운동군, 당뇨군, 당뇨운동군으로 분류하여 각 군당 7마리씩 배정하였다. 당뇨는 streptozotocin을 복강에 주사하여 유발하였다. 운동군은 분당 8 m의 속도로 하루 30분씩 주 5회, 총 12주 동안 트레드밀 운동을 실시하였다. 본 연구의 결과, 당뇨 유발 흰쥐의 망막에서 세포사멸 인자인 terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL)-양성 세포수 그리고 caspase-3, cytochrome c, Bax의 발현이 증가되었으며, 항 세포사멸 인자인 Bcl-2의 발현은 감소되었다. 트레드밀 운동은 TUNEL-양성 세포수 그리고 caspase-3, cytochrome c, Bax의 발현을 감소시켰으며, Bcl-2의 발현은 증가시켰다. 본 실험의 결과, 당뇨에 의한 망막의 세포사멸 증가에 트레드밀 운동이 억제 작용을 나타내었으며, 따라서 트레드밀 운동은 당뇨 환자들에서 후유증을 경감시키는데 효과적인 치료법임을 알 수 있었다.