• The Plant Pathology Journal
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• 제27권2호
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• pp.187-190
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• 2011

Symptoms induced in the leaves of strawberry (Fragaria x ananassa Duch.), 'Seolhyang' and 'Eyeberry', were mosaic, distortion and black colored edge on leaves at Nonsan area, one of the important production areas in Korea. Electron microscopy by quick-dip revealed the flexuous rod-shape particles having about 550-600 nm length. Cytoplasmic inclusion bodies composed of aggregated virus particles were observed frequently in mesophyll parenchyma and epidermal cells for the leaves of strawberry. The specific primers amplifying products of 635 bp and 729 bp were developed for RT-PCR detection of Strawberry mild yellow edge virus (SMYEV). Nucleotide identity of the CP gene of SMYEV was 92.8-99.2% with those of other SMYEV isolates from Gen-Bank database.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 춘계학술대회
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• pp.80-80
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• 2019

This study describes an efficient and widely applicable droplet-vitrification following cryopreservation for shoot tips of (Fragaria x ananassa DUCH. cvs. 'Derunoka' and 'Jumbo pure berry'. The shoot tips of strawberry were precultured in Murashige and Skoog (MS) liquid medium supplemented with sucrose (0.3-0.7M). Precultured explants were treated with loading solution (LS, C4) containing glycerol 17.5% and sucrose 17.5% for 40 min and exposed to dehydration solution (B1) containing 50% of glycerol and 50% of sucrose for 60 min at $25^{\circ}C$, and then transferred onto droplets containing $2.5{\mu}l$ PVS3 on sterilized aluminum foils ($4cm{\times}0.5cm$) prior to direct immersion in liquid nitrogen (LN) for 1 h. The highest regeneration rate (%) was obtained when shoot tips were precultured with treatment-2 (exposing of shoot tips to MS + 0.3M Sucrose for 30 h and then treated with MS+0.5 M sucrose for 16 h) at $25^{\circ}C$ in both the cultivars. The viability of cooled samples, followed by culturing on MS medium for 4 weeks was 77.8% and 60.0% for 'Derunoka' and 'Jumbo pure berry', respectively. This result shows droplet-vitrification would be a promising method for cryobanking strawberry germplasm.

• Journal of Plant Biotechnology
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• 제2권3호
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• pp.115-121
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• 2000

A cDNA clone encoding chloroplast triosephosphate isomerase (TPI-cp) was isolated from strawberry fruit cDNA library. Sequence analyses indicated that the cDNA contains an open reading frame of 314 amino acids (33.5 kDa) composed of a transit peptide (59 amino acids) in amino terminal region and mature protein (255 amino acids). The existence of transit peptide in the deduced amino acid sequence implies that it encodes a chloroplast isoform. The protein sequence is more similar to other plant chloroplast isoforms than cytosolic isoforms. RNA blot analysis indicated that its expression is ubiquitous in examined five tissues, flowers, leaves, petioles, roots and fruits, and shows differential pattern according to fruit ripening. Genomic DNA blot analysis showed that TPI-cp is encoded by multiple genes in strawberry. Through sequence comparison and phylogenetic tree construction, TPI-cp is distinctively grouped into dicot and chloroplast isoforms.

• 원예과학기술지
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• 제28권5호
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• pp.777-782
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• 2010

'설향' 딸기의 영양생리를 구명하기 위한 연구의 일환으로 Hoagland 용액의 질소 수준을 변화시킨 용액으로 재배하면서 생장 및 무기원소 흡수에 미치는 영향을 구명하기 위해 본 연구를 수행하였다. 본 연구에서 적용한 관비용액의 질소 수준($meq{\cdot}L^{-1}$)은 0(${NO_3}^-$), 2.5(${NO_3}^-$), 5(${NO_3}^-$), 10($7.5{NO_3}^-$ + $2.5{NH_4}^+$), 그리고 15($10{NO_3}^-$ + $5{NH_4}^+$) 였다. 정식 120일 후의 지상부 생체중과 엽병추출액의 질산태질소 농도는 관비용액의 질소 시비수준이 높아짐에 따라 각각 2차곡선회귀적(y=7.10+2.668x-$0.115x^2$, $R^2=0.7983^{***}$) 또는 직선적(y=26.14+5.245x, $R^2=0.6083^{***}$)으로 증가하였다. 지상부 건물중과 건물내 질소 함량도 각각 2차곡선회귀적(y=2.140+0.492x-$0.022x^2$, $R^2=0.6110^{***}$) 또는 직선적(y=0.569+0.033x, $R^2=0.6952^{***}$)으로 증가하는 반응을 보였다. 식물체의 지상부 건물중과 생체중을 고려할 때 본 연구에서 적용한 관비용액의 질소농도 중 5meq.L-1 처리의 생장이 가장 우수하였지만 '설향' 딸기의 최적 생장을 위해 $5meq{\cdot}L^{-1}$ 처리 용액을 변화시켜야 하며 추후 관련 연구가 필요하다고 판단하였다.

• Journal of Crop Science and Biotechnology
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• 제11권2호
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• pp.107-110
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• 2008

A high-efficiency, reproducible somatic embryogenesis system for strawberry cultivar Chandler was developed. Thirty-one somatic embryos per explant(max no.) were recorded in leaf discs which were cultured on medium containing MS salts+$B_5$ vitamins+2% glucose+4.0 mg $1^{-1}$TDZ(Thidiazuron) and incubated at $10{\pm}1^{\circ}C$ under darkness for one week followed by three weeks under 16-h photoperiod. The scanning electron microscopic(SEM) ontogeny revealed the normal development of somatic embryos from globular to heart-shaped and dissection microscopy from torpedo-shaped to cotyledonary-stage embryos. The maximum germination percentage of 48% could be obtained on MS medium containing kinetin(1.0 mg $1^{-1}$) and the maximum survival percentage(79%) of plantlets after four weeks was found to be in the mixture of vermiculite, peatmoss, and soilrite(1:1:1).

• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.1617-1617
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• 2001

It is well known now that near infrared spectroscopy (NIRS) is a fast, no destructive, and inexpensive analytical technique that could be used to classify, identify, and authenticate a wide range of foods and food items. Therefore, the main aims of this study were to provide a new insight into the authentication of five strawberry (Fragaria x ananassa) varieties and to correlate them with geographical zones and the propagating methods used. Three weeks plants of five different strawberry varieties (F. x ananassa Duch. cv Camarosa, Seascape, Chandler, F. Chiloensis, and F. Virginiana) were cultivated in vitro first and then transferred to pots with special soil, and grown in a greenhouse at CINVESTAV, all varieties were acquired from California (USA). After 18 months, ten leaves from each variety were collected. Transmission spectra from each leave were recorded over a range of 10, 000-4, 000 cm$-^{1}$, 32 scans of each strawberry leave were collected using a resolution of 4 cm$-^{1}$ with a Paragon IdentiCheck FT-NIR System Spectrometer. Triplicates of each strawberry leave were used. All spectra were analyzed using principal component analysis (PCA) and soft independent modeling class analogy (SIMCA). The optimum number of components to be used in the regression was automatically determined by the software. Camarosa was the only variety grown from the same shoot but propagated by a different method (direct or in vitro). Five different classes (varieties) or clusters were observed among samples, however, larger inter class distances were presented by the two wildtype samples (F. Chiloensis and F. Virginiana). Camarosa direct and Camarosa in vitro displayed a small overlapping region between them. On the other hand, Seascape variety presented the smallest rejection percentage among all varieties (more similarities with the rest of the samples). Therefore, it can be concluded that the application of NIRS technique allowed the authentication of all strawberry varieties and geographical origin as well. It was also possible to form subclasses of the same materials. The results presented here demonstrate that NIRS is a very powerful and promising analytical tool since all materials were authenticated and classified based on their variety, origin, and treatment. This is of a tremendous relevance since the variety and origin of a plant material can be established even before it gives its typical fruit or flower.

• 생물환경조절학회지
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• 제28권4호
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• pp.429-437
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• 2019

본 연구는 '매향' 딸기 런너의 삽목번식시 차광 및 가습에 따른 효과를 확인하기 위하여 수행하였다. 런너 삽수를 2017년 11월 23일에 삽목한 후 대조구(무가습+무차광, C), 무가습+흰색 한랭사 차광 55%(W55), 무가습+검은색 차광막 55%(B55), 무가습+멀칭비닐 차광 100%(B100), 가습+무차광(CH), 가습+흰색 한랭사 차광 55%(W55H), 가습+검은색 차광막 55%(B55H) 및 가습+멀칭비닐 차광 100%(B100H)를 처리하였다. 대조구(무가습+무차광, Control), 무가습+멀칭비닐 100% 차광(Shading), 가습+무차광(Fogging)의 처리를 두고 육묘하였다. 지상부의 생육은 처리간 유의적인 차이가 없었다. 전반적인 지하부의 생육에 있어 가습 처리가 무가습 처리에 비해 우수하였다. 삽목 26일 후 지하부의 생체중과 건물중의 경우 가습+무차광 처리에서 가장 우수하였다. 삽목 26일 후 근장의 경우 가습+흰색 한랭사 차광 55% 처리구 및 가습+검은색 차광막 55% 처리구에서 가장 우수하였다. 이상의 결과를 종합하면 딸기 '매향'의 삽목 번식시 가습 및 차광 유무에 따라 삽목묘의 형태형성에 영향을 주는 것으로 판단된다. 또한 이러한 결과는 삽목번식 방법을 수행하는 다른 작물에도 응용될 수 있을 것으로 판단된다.

• 생물환경조절학회지
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• 제17권3호
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• pp.238-245
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• 2008

마그네슘 시비농도를 인위적으로 조절하여 '매향' 딸기를 관비재배하면서 Mg의 시비수준이 생장과 결핍증상 발현에 미치는 영향을 구명하고, 건전생육을 유지할 수 있는 식물체 및 토양의 한계농도를 밝히기 위하여 본 연구를 수행하였다. 마그네슘 결핍증상은 하위엽에서 발생하였으며, 초기에 하위엽의 엽맥 사이에서 반점 형태의 황화현상이 나타난 후 점차 반점 부위가 확산되어 엽맥간 황화현상으로 발전하였다 또한 증상이 심해지면서 엽맥 사이가 검게 변하고, 하위엽 선단의 갈변 및 괴사하는 증상이 발생하였다. Mg시비농도를 조절하여 관비하고 정식 120일 후에 지상부 생육을 조사한 결과 Mg 1.0 또는 2mM의 처리에서 생육이 우수하였으며, 0.5 이하나 4mM 이상으로 Mg 시비농도를 조절한 처리의 생장이 저조해지는 경향이었다. 건물중은 마그네슘 시비농도에 대하여 3차 곡선회귀적인 반응을 보였으며 식물체당 약 8.2g의 건물중을 생산할 때 정점이 형성되었다. 최대 생장량의 90%를 최저 한계점으로 간주하면 식물체당 7.4g 이상의 건물중을 생산하기 위해서는 Mg 함량이 $0.30\sim0.65%$의 범위에 포함되도록 시비해야 하며, 최적 시비농도는 약 2mM 이라고 판단하였다. 또한, 최대 생산량인 식물체당 생체중 36.2g의 90%를 최저 및 치고 한계점으로 간주 할 경우 엽병 추출액의 Mg농도가 $19\sim40mg{\cdot}kg^{-1}$의 범위에 포함되도록 시비해야 한다고 판단하였다.

• 생물환경조절학회지
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• 제17권3호
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• pp.231-237
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• 2008

국내육성 딸기 신품종인 '매향'을 Ca 농도를 조절한 관비용액으로 재배하면서 결핍증상의 특징과 결핍증상을 유발하는 건물중 및 생체즙액내 한계농도를 구명하기 위하여 본 연구를 수행하였다. Ca 결핍증상은 신엽에서 발생하였고, 신엽의 엽맥 부분이 갈변하는 증상과 함께 신엽이 기형화되면서 선단부가 괴사하는 증상이었다. 정식 후 120일에 지상부의 생육을 조사한 결과 엽수, 엽장, 엽병장, 생체중 및 건물중은 4.5mM과 6mM 시비구에서 생육이 우수하였고, 3mM 이하나 9mM의 칼슘 농도에서 생장량이 적어 2차곡선회귀가 성립하였고 경향이 뚜렷하였다. 식물체당 건물중 4.9g에서 2차 곡선회귀의 정점이 형성되었으며(y=2.4026+1.0209x-$0.0985x^2$, $R^2=0.3546^{***}$), 최대 생장량의 90% 이상 생장량을 최저 한계점으로 설정하면 식물체당 약 4.4g이상의 건물중을 생산해야 하며 건물중에 기초한 Ca함량이 $1.6\sim2.25%$의 범위에 포함 되도록 시비량을 조절해야 할 것으로 판단하였다. 생체중도 Ca 시비농도에 대하여 3차 곡선회귀적인 반응을 보였으며(y=9.273+4.882x-$0.4245x^2$, $R^2=0.4935^{***}$), 식물체당 239에서 정점이 형성되었다. 최대 생장량의 90% 이상을 확보하려면 엽병 추출액의 Ca 농도가 $63\sim79mg{\cdot}kg^{-1}$의 범위에 포함되도록 Ca 시비농도를 조절해야 한다고 판단하였다.

• 한국자원식물학회지
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• 제35권6호
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• pp.825-830
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• 2022

"Doha" is a new strawberry (Fragaria x ananassa Duch.) variety, which was released by the Highland Agriculture Research Institute in 2021. The "Doha" variety originates from a 2014 cross between "Saebong No. 3" and "Yeolha," both of which exhibited excellent ever-bearing characteristics, including continuous flowering and large fruits under long-day and high temperature conditions. This new cultivar was initially named "Saebong No. 13" after examining its characteristics and productivity during summer cultivation between 2015 and 2018. After regional adaptability tests, "Doha" was selected from "Saebong No. 13" as an elite cultivar. The general characteristics of "Doha" include spreading, elliptic leaves, and strong growth. The fruits are long and conical and of a red color. The plant height of "Doha" was similar to that of "Goha," but the number of leaves was lower. The number of flower clusters of "Doha" was 8.6, which was 2.8 fewer than that of the control variety, "Goha," with 11.4. The average fruit weight of "Doha" was 13.9 g, which was 4.9 g heavier than that of "Goha." The fruit hardness of "Doha" was 35.5 g·mm^-2, which was 9.4 g·mm^-2 harder than that of "Goha." The marketable yield of "Doha" was 26,971 kg·ha^-1, 125% more than that of "Goha" with 21,479 kg·ha^-1. The findings of this study suggest that "Doha" is a hard fruit and high-yielding variety of ever-bearing strawberries that could increase farming income when distributing to farmers.