• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2008년도 추계 학술발표회 제20권2호
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• pp.97-100
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• 2008

최근 들어 고강도 철근의 사용이 증가하고 있지만, 지진위험이 있는 지역의 내진구조물에 있어서는 고강도 철근의 취성적 성질로 인해 그 사용이 제한되어 왔다. 그러나, 철근의 연성의 변화가 기둥의 부재레벨의 연성도에 미치는 영향에 대해서는 많은 연구가 없는 실정이다. 특히 고강도 철근을 사용하는 경우 철근의 연성의 변화로 인해 부재의 소성힌지 길이가 달라질 것으로 예상되지만, 기준의 소성힌지길이 산정식은 철근 등 재료의 특성을 고려하지 못하고 있다. 지진하중을 받는 철근콘크리트 기둥의 소성힌지길이는 실험을 통해서 측정하기는 어려움이 많다. 따라서, 본 논문에서는 해석적인 방법을 통하여 재료레벨의 연성, 특히 철근의 연성이 소성힌지의 생성에 미치는 영향을 평가하고, 그 영향을 고려한 소성힌지길이 산정식을 제안하고자 한다.

• 대한기계학회논문집
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• 제16권4호
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• pp.684-695
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• 1992

본 연구에서는 3차원 형상의 금형에 의한 박판금속 성형 공정을 에너지법으로 해석함에 있어 스윕곡면(SWeep surface)을 통해 기하학적 형상을 효과적으로 표현하는 방법을 제안하고, 이를 직사각형 격막의 정수압벌징의 해석에 으용하여 이에 의한 결 과를 이미 보고된 자료와 비교함으로써 일반적인 박판 성형공정을 해석하는데 본 이론 의 적용 가능성에 대하여 논의하고자 한다.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2004년도 제5회 압연심포지엄 신 시장 개척을 위한 압연기술
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• pp.307-316
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• 2004

This study examined the cause of the wrinkle defect which is frequently encountered in wire rod rolling of low carbon steel$(C0.08\~0.13wt.\%)$. Even a small defect on the surface of rolled bars can easily develop into fatal cracks during cold heading process of low carbon steel, and it is therefore necessary to minimize inherent defects on the surface of hot rolled bars. Hot rolling process of low carbon steel was analyzed to identify the cause of the wrinkle defect in conjunction with FE analysis. The integrated analysis revealed that the wrinkle defect initiated in the first stage of rolling, and it was at the billet edge where severe deformation and drastic temperature drop were present. To elucidate the micro-mechanical mechanism of the wrinkle defect, hot compression tests were carried out at various temperatures and strain rates using Gleeble-3800. The surface profile of the each other compressed specimens was compared, and rough surface lines were observed at relatively low temperatures. Those surface defects can develop into wrinkles during multi-pass rolling. To control the wrinkle defect in rolling, it is necessary to design an adequate caliber which can minimize the loss of ductility, and thereby prevent flow localization. To use the result of this study fur other steels, the quantitative measure of the wrinkle defect and flow localization parameter should be proposed.

• 한국강구조학회 논문집
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• 제14권1호
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• pp.79-85
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• 2002

본 연구에서는 기존에 사용된 H-Beam 교량을 편향부와 외부 긴장력을 도입하여 보강효과에 의하여 지간을 증가시킬 수 있고 단면을 축소할 수 있다는 근거를 제시하였고, 유연도법을 이용하여 편향부의 효과에 의한 추가 긴장량을 산정하였다. 또한, 실험변수(긴장량, Tendon의 개수, 편향부의 유 무)에 의해서 표준실험체(기존 H-Beam)와 외부 긴장된 H-Beam 등 5개의 실험체를 제작하여 휨 실험을 실시하여 외부 긴장된 H-Beam의 역학적 거동을 연구하였다. 특히, 보강효과 및 편향부의 효과를 실험적으로 검증하였다.

• 대한기계학회논문집A
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• 제38권8호
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• pp.899-904
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• 2014

자유벌징에 있어서 성형 높이를 최대화하기 위하여 블랭크의 두께 분포를 최적화 하였으며, 등가정하중을 이용한 구조최적화법을 사용하였다. 두께형상은 부드러운 곡선으로 나타내기 위하여 베지어곡선을 사용하였고 제어점의 위치가 설계변수이며, 최대 변형률을 일정 값으로 제한하였다. 사용된 소재는 인코넬 718 이며 최적화된 두께분포로 가공된 블랭크를 이용한 자유벌징 시험을 수행하여 평판형 블랭크를 사용한 결과보다 22% 더 높은 성형 높이를 얻었다. 최적화결과에서 예측된 변형형상, 정점에서의 변형 경향, 두께분포가 실험에서 얻은 결과와 유사하여 최적화 과정의 유효성을 입증하였고, 최적화 결과가 실제 구현될 수 있음을 검증하였다.

• 한국응용과학기술학회지
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• 제28권3호
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• pp.273-283
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• 2011

The objective of this study was to determine the feeding effect of a diet containing Cordyceps with fly pupa on the changes in blood lipid profile from broiler chickens, fatty acids and thiobarbituric acid reactive substances (TBARS) in chicken meat. A total of 360 chicks (Ross strain 308) were divided on hatch day into four treated groups with respect to a 35-day feeding regimen: T1 (control group), T2 (2.0% Cordyceps with fly pupa), T3 (3.5% Corceps with fly pupa) and T3 (5.0% Cordyceps with fly pupa). Blood triacylglyceride and total cholesterol level was significantly decreased by 5.32-10.63% and 9.23-12.62%, respectively, in groups T2, T3 and T4 when compared to T1 (p<0.05). Water holding capacity was significantly highest in T2 (p<0.05), while there were no significant differences among groups T2, T3 and T. In chicken meat, the ratio of saturated fatty acid to unsaturated fatty acid was high in the T3 and T4 groups, the ratio of n-6 to n-3 fatty acid was low in the T2, T3 and T4 groups and oleic acid (18:1n-9) was high in the T2, T3 and T4 groups, when compared to T1 (p<0.05). TBARS tended to increase according to the storage time (in days), and was significantly lower in the chicken thigh muscles with skin in groups T2, T3 and T4 as compared to T1 (p<0.05). These results suggest that a diet containing 2.0-3.5% of Cordyceps with fly pupa may decrease blood lipid and improve both the shelf-life and quality of broiler chicken meat.

• Asian-Australasian Journal of Animal Sciences
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• 제15권8호
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• pp.1121-1127
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• 2002

Sixteen crossbred (Bos indicus${\times}$Bos taurus) calves were randomly distributed in two groups (NP and LP) of eight calves each to study the effect of restricted (75%) protein supply on growth and immuno-biochemical response as an indicator of production and health of under-nourished animals during 3 to 9 months of age. The normal requirement of protein was provided to group NP and a less of 25% to group LP through calculated amount of concentrate and roughage in their daily ration. Assessment was made for weekly change in live weight, periodic alteration in blood metabolites and immunological status at six months of age in calves. An initial (during 3 to 6 months of age) depression (p<0.05) in growth was seen in low protein fed group (LP) compared to NP, which became non-significant in the later period of life (6 to 9 months of age). There was no significant effect on haemoglobin, total protein, albumin and globulin concentration except that of urea, which was decreased significantly (p<0.05) in animals fed on low protein diet ($19.83{\pm}1.25$ vs $25.93{\pm}1.29mg/dl$). The treatment effect that was seen in different periods of life was not uniform for other parameters except for urea, which showed a regular depression in LP compared to NP. The assessment of immunological status by indirect haemagglutination (IHA) test against Pasteurella multocida (P52 strain) was considerably (p<0.05) reduced in animals on LP ration compared to those on NP. It is thus argued that with poor nutrition (low protein) and state of compromised immunological response the production and health of the animals will be adversely affected.

• Journal of Microbiology and Biotechnology
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• 제17권9호
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• pp.1498-1503
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• 2007

To identify novel crystal proteins, Bacillus thuringiensis 2385-1 was isolated from Korean soil samples and characterized. The H-serotype of 2385-1 was identical to that of subsp. kenyae (H4a4c), and its crystal toxin was bipyramidal-shaped. However, 2385-1 showed a much higher toxicity towards Plutella xylostella and Spodoptera exigua larvae than subsp. kenyae. In addition, the crystal protein profile and plasmid DNA pattern of 2385-1 differed from those of subsp. kenyae. To verify the crystal protein gene types of 2385-1, a PCR-RFLP analysis was performed, and the results revealed that 2385-1 contained two novel cry1-type crystal protein genes, cryl-5 and cry1-12, in addition to the crylJal gene. The deduced amino acid sequences of cryl-5 and cry1-12 showed a 97.9% and 75.7% sequence similarity with the CrylAb and CrylJa crystal proteins, respectively. Among the novel crystal proteins, Cry1-5 showed a high toxicity towards P. xylostella and S. exigua larvae. In conclusion, B. thuringiensis 2385-1 is a new isolate in terms of its gene types, and should be a promising source for an insecticide to control lepidopteran larvae.

• Journal of Microbiology and Biotechnology
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• 제20권11호
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• pp.1555-1562
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• 2010

Development of the strain and the fermentation process of Hansenula polymorpha was implemented for the production of ${\gamma}$-linolenic acid ($GLA,\;C18:3{\Delta}^{6,9,12}$), an n-6 polyunsaturated fatty acid (PUFA) that has been reported to possess a number of health benefits. The mutated ${\Delta}^6$-desaturase (S213A) gene of Mucor rouxii was expressed in H. polymorpha under the control of the methanol oxidase (MOX) promoter. Without the utilization of methanol, a high-cell-density culture of the yeast recombinant carrying the ${\Delta}^6$-desaturase gene was then achieved by fed-batch fermentation under glycerol-limited conditions. As a result, high levels of the ${\Delta}^6$-desaturated products, octadecadienoic acid ($C18:2{\Delta}^{6,9}$), GLA, and stearidonic acid ($C18:4{\Delta}^{6,9,12,15}$), were accumulated under the derepression conditions. The GLA production was also optimized by adjusting the specific growth rate. The results show that the specific growth rate affected both the lipid content and the fatty acid composition of the GLA-producing recombinant. Among the various specific growth rates tested, the highest GLA concentration of 697 mg/l was obtained in the culture with a specific growth rate of 0.08 /h. Interestingly, the fatty acid profile of the yeast recombinant bearing the Mucor ${\Delta}^6$-desaturase gene was similar to that of blackcurrant oil, with both containing similar proportions of n-3 and n-6 essential fatty acids.

• The Plant Pathology Journal
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• 제34권4호
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• pp.269-285
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• 2018

Bacterial leaf blight is a major disease of eucalypt, especially under nursery conditions. Different bacterial species have been associated with the disease in several countries, and despite its importance worldwide, it is not clear to date whether similar disease symptoms are caused by the same or by different etiological agents. In this study, 43 bacterial strains were isolated from blighted eucalypt leaves collected in different geographic areas of Brazil and inoculated onto a susceptible eucalypt clone. Polyphasic taxonomy, including morphological, physiological, biochemical, molecular, and pathogenicity tests showed that only certain strains of Xanthomonas axonopodis caused symptoms of the disease. Strains varied in their aggressiveness, but no correlation with geographic origin was observed. MLSA-based phylogenetic analysis using concatenated dnaK, fyuA, gyrB and rpoD gene sequences allocated the strains in a well-defined clade, corresponding to Rademarker's group RG 9.6. Inoculation of nineteen plant species belonging to seven botanical families with representative strain LPF 602 showed it to be pathogenic only on Eucalyptus spp, and Corymbia spp. Based on distinct biochemical and pathogenic characteristics that differentiate the eucalypt strains from other pathovars of the X. axonopodis species, here we propose their allocation into the new pathovar X. axonopodis pv. eucalyptorum pv. nov.