• 소성∙가공
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• 제4권1호
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• pp.9-16
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• 1995

Model material technique, which requires only the small space of experimental set-up and low cost for experiment, is used to estimate the deformed profile and the forging load in rotary forging. The materials and working conditions are determined to satisfy the similitude conditions between the model test and the prototype test. The model material of the so-called plasticine and the mild steel are chosen as specimens, and they represent almost the same value of strain gardening exponent in the stress-strain relationship. Lubricant in the model test is also carefully selected so that it gives the same frictional conditions at the tool-specimen interface. Experiments for two kinds of specimens are carried out in each testing equipment at room temperatue. From the experiments the deformed dimensions and the forging loads are measured and compared with each other by using the simulation coefficients. It is shown that there are good agreements between the model test and the prototype test. Finally, for verifying the availability of the model material technique this mathod is applied to forging of bevel gear product. the good result is obained which can demonstrate that the model material technique is very efficent for estimating or developing a new process.

• 대한기계학회논문집
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• 제9권6호
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• pp.725-731
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• 1985

본 논문에서는 Chen, Kobayashi에 의한 강소성 유한요소법 프로그램을 이용하 여 마찰조건 및 형상비(H$_{O}$/D$_{O}$)가 0.75, 1.0, 1.5에 따른 금형 접촉면상의 응력분포 및 반경방향 증분량 그리고 번형률 분포 등을 고찰하고 실제로 알루미늄과 .alpha.-황동의 두 종류 재료를 사용하여 이론과 동일한 마찰조건과 형상비에 대해 실험함 으로써 그 변형 특성을 F.E.M. 결과와도 비교 고찰하였다. 또한 시편 실험에서 파괴 시까지 실험하여 그 형상도 살펴보았으며 이전 연구와도 비교하여 보았다.

• Journal of Microbiology and Biotechnology
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• 제25권10호
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• pp.1680-1686
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• 2015

Inhibition of 4-nitroquinoline-1-oxide (4-NQO) genotoxicity by a probiotic strain of Lactobacillus rhamnosus (IMC501) was assessed by the prokaryotic short-term bioassay SOSChromotest, using Escherichia coli PQ37 as the target organism. Results showed the ability of strain IMC501 to rapidly and markedly counteract, in vitro, the DNA damage originated by the considered genotoxin. The inhibition was associated with a spectroscopic hypsochromic shift of the original 4-NQO profile and progressive absorbance increase of a new peak. IR-Raman and GC-MS analyses confirmed the disappearance of 4-NQO after contact with the microorganism, showing also the absence of any genotoxic molecule potentially available for metabolic activation (i.e., 4-hydroxyaminoquinoline-1-oxide and 4-nitrosoquinoline-1-oxide). Furthermore, we have shown the presence of the phenyl-quinoline and its isomers as major non-genotoxic conversion products, which led to the hypothesis of a possible pattern of molecular transformation. These findings increase knowledge on lactobacilli physiology and contribute to the further consideration of antigenotoxicity as a nonconventional functional property of particular probiotic strains.

• Journal of Microbiology and Biotechnology
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• 제24권10호
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• pp.1445-1454
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• 2014

Severe acute respiratory syndrome (SARS), a disease that spread widely in the world during late 2002 to 2004, severely threatened public health. Although there have been no reported infections since 2004, the extremely pathogenic SARS coronavirus (SARS-CoV), as the causative agent of SARS, has recently been identified in animals, showing the potential for the re-emergence of this disease. Previous studies showed that 27 single nucleotide polymorphism (SNP) mutations among the spike (S) gene of this virus are correlated closely with the SARS pathogenicity and epidemicity. We have developed a SNP DNA microarray in order to detect and genotype these SNPs, and to obtain related information on the pathogenicity and epidemicity of a given strain. The microarray was hybridized with PCR products amplified from cDNAs obtained from different SARS-CoV strains. We were able to detect 24 SNPs and determine the type of a given strain. The hybridization profile showed that 19 samples were detected and genotyped correctly by using our microarray, with 100% accuracy. Our microarray provides a novel method for the detection and epidemiological surveillance of SARS-CoV.

• 접착 및 계면
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• 제4권2호
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• pp.1-9
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• 2003

Epoxy-amine liquid prepolymers are extensively applied onto metallic substrates and cured to obtain painted materials or bonded joint structures. Overall performances of such systems depend on the created interphase between the organic layer and the substrate. When epoxy-amine liquid mixtures are applied onto more or less hydrated metallic oxide layer, concomitant amine chemical sorption and hydroxide dissolution appear lending to the chelate formation. As soon as the chelate concentration is higher than the solubility product, these species crystallize as sharp needles. Moreover, intrinsic and thermal residual stresses are developed within painted or bonded systems. When residual stresses are higher than the organic layer/substrate adhesion, buckling, blistering, debonding may occur leading to a catastrophic drop of system performances. Practical adhesion can be evaluated with either ultimate parameters (Fmax or Dmax) or the critical strain energy release rate, using the three point flexure test (ISO 14679-1997). We observe that, for the same system, the ultimate load decreases while residual stresses increase when the liquid/solid time increases. Ultimate loads and residual stresses depend on the metallic surface treatment. For these systems, the critical strain energy release rate which takes into account the residual stress profile and the Young's modulus gradient remains quite constant whatever the metallic surface treatment was. These variations will be discussed and correlate to the formation mechanisms of the interphase.

• 한국추진공학회:학술대회논문집
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• 한국추진공학회 2001년도 제16회 학술발표회 논문초록집
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• pp.91-94
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• 2001

A structural test of the wind turbine rotor blade must be required to evaluate the uncertainty in design assessment due to use of material, design concepts, production processes and so on, and the possible impact on the structural integrity. In the full-scale static strength test, the measuring parameters are strain, displacements, loads, weight and the center of gravity. There are test equipments, measuring sensors, a test rig and fixtures to obtain measuring parameters. In order to simulate the aerodynamics load, the three-point loading method instead of the one-point loading method is applied. There is slightly some difference between the measured results and the predicted results with the reference fiber volume fraction of 60%. However, the agreement between the measured results and the predicted results with the actual fiber volume fraction of 52.5% is good. Even though a slightly non-linearity from 80% loading to 100% loading, a linear static solution is sufficient for the design purpose as the amount of the non-linearity is relatively small. Comparison between measured and predicted strain results at the maximum thickness positions of the blade profile for 0.236R(5.56m), 0.493R(11.59m) and 0.574R(13.43m), under 20%, 40%, 60%, 80% and 100% loadings for the upper part of the blade. The predicted values are in good agreement with the measured values.

• 한국버섯학회지
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• 제16권3호
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• pp.140-146
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• 2018

Pleurotus eryngii is one of the most commercially important mushrooms cultivated in Korea. However, the shelf-life of the fruiting body is short, limiting its export. A new hybrid strain H17 of P. eryngii was developed to extend the shelf-life by mono-mono crossing between monokaryotic strains derived from DanBi and KNR2774. Although the cultivation period of H17 was slightly longer than that of the reference cultivar Kenneutari No.2, the quality did not change and remained normal after a period of 65.0 days at $4^{\circ}C$. This result was significantly different from that of the reference cultivar Kenneutari No.2. Analysis of the genetic characteristics of the new hybrid strain H17 revealed a different profile from that of the parental and reference cultivars when random amplification of polymorphic DNA (RAPD) primers was used. These results demonstrate that H17 is a new cultivar with improved storability after harvesting.

• 대한수의학회지
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• 제49권2호
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• pp.127-133
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• 2009

Salmonella enterica subsp. enterica serovar gallinarum (S. gallinarum) is the agent of fowl typhoid, and the 9R vaccine is a commercial live vaccine for the prevention of fowl typhoid. The aim of this study was to assess the safety and immunogenicity of different brands of S. gallinarum 9R vaccine used in commercial laying chickens in Korea. All 9R strains originated from three different brands showed the same pattern in the biochemical and serological properties, and pulsed field gel electrophoresis (PFGE) profile. But, there was a difference in rhamnose fermentaion, agglutination with Salmonella group $D_1$ antiserum and PFGE pattern between 9R vaccine strain and field S. gallinarum isolates. In laboratory and field trials for assesment of safety and immunogenicity of 9R vaccine, all of the three 9R vaccines showed the same safety in commercial laying chickens. In addition, there was a significant difference between the vaccinated and unvaccinated control groups in mortality and the re-isolation rate of the challenge strain from the tissues (p < 0.05), and no difference by the brands of 9R vaccine. The results from this study indicated that all three different brands of S. gallinarum 9R vaccine showed highly protection against mortality and organ invasion in commercial laying chickens exposed to virulent strains of S. gallinarum.

• Biotechnology and Bioprocess Engineering:BBE
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• 제7권2호
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• pp.105-111
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• 2002

The biological removal of 2,4,6-trinitrotoluene (TNT) was studied in a bench-scale bioreactor using a bacterial culture of strain OK-5 originally Isolated from soil samples contaminated with TNT. The TNT was completely removed within 4 days of incubation in a 2.5 L bench-scale bioreactor containing a newly developed medium. The TNT was catabolized in the presence of different supplemented carbons. Only minimal growth was observed in the killed controls and cultures that only received TNT during the incubation period. This catabolism was affected by the concentration ratio of the substrate to the biomass. The addition of various nitrogen sources produced a delayed effect for the TNT degradation. Tween 80 enhanced the degradation of TNT under these conditions. Two metabolic intermediates were detected and identified as 2-amino-4, 6-dinitrotoluene and 4-amino-2, 6-dinitrotoluene based on HPLC and GC-MS analyses, respectively. Strain OK-5 was characterized using the BIOLOG system and fatty acid profile produced by a microbial identification system equipped with a Hewlett Packard HP 5890 II gas chromatograph. As such, the bacterium was identified as a Stenotrophomonas species and designated as Stenotrophomonas sp. OK-5.

• 한국구조물진단유지관리공학회 논문집
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• 제5권2호
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• pp.111-120
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• 2001

This paper aims at estimating instantaneous prestress losses by measuring the actual prestress forces in prestressed concrete (PSC) box girder bridges. Measurement were taken to study initial prestress losses such as friction losses and slip losses. A new strain gauge system was developed to measure strains in internal tendons. The system was installed on a total of 20 tendons in a PSC box girder bridges. The variation of prestress forces were monitored during prestressing tendon and after prestress transfer. The prestress losses are also calculated including friction losses and slip losses. The measured data were compared with the theoretical values. The result shows that the measured prestress forces agree well with the theoretical values. It is shown that prestress force of each strand in the same tendon is a bit different. This study also shows that prestress losses of continuity tendons during prestress transfer are significantly different each other, which results from the variety of buttress location and tendon profile. The present study provides realistic information on the estimation of actual prestress forces and losses in PSC box girder bridges.