• 한국지반공학회논문집
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• 제27권2호
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• pp.43-52
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• 2011

조립토로 구성된 지반구조물의 안정성은 전단변형에 따른 전단영역의 특성변화에 의해 영향을받는다. 본 연구의 목적은 전단파와 전기비저항 및 콘 선단저항력을 이용하여 직접전단실험시 발생하는 전단영역의 특성을 파악하는 것이다. 전단영역의 특성을 파악하기 위하여, 아크릴 재질로 직접전단상자를 제작하였으며, 직접전단상자의 벽면에는 전단파와 전기비저항의 측정을 위하여 벤더엘리먼트와 전기비저항 프로브를 설치하였다. 벤더엘리먼트와 전기비저항 프로브는 전단영역과 비전단영역에 각각 설치되어, 전단변형에 따른 조립토의 거동을 비교할 수 있도록 하였다. 또한, 콘 선단저항력을 측정할 수 있도록 개발한 마이크로 콘을 첨두강도상태와 잔류강도상태에서 관입하여 깊이에 따른 시료의 강도분포를 관찰하였다. 실험결과, 바닥부근과 하부전단영역에서는 전단변형에 따른 전단파 속도는 일정하였지만, 상부전단영역에서의 전단파 속도는 증가하였다. 또한, 바닥부근의 전기비저항은 변화가 없는 반면, 하부전단영역에서 전기비저항은 상대밀도에 따라 수직변형률과 반비례관계로 나타났다. 콘 선단저항력의 변화도 전단파 속도의 변화와 유사하게 상부전단영역에서 큰 변화가 관찰되었다. 본 논문에서 제시한 직접전단실험시 전단파와 전기비저항을 관찰하는 것과 실험완료 후 콘 관입실험은 조립토의 전단영역 특성을 파악하기 위한 매우 효과적인 방법이 될 수 있음을 보여준다.

• 한국지반공학회논문집
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• 제27권1호
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• pp.77-86
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• 2011

건설 프로젝트의 대형화와 시공장소의 다양화에 따라 지반 조사 내용이 다양해지고 있다. 더욱이 날로 엄격해지는 시공조건으로 인해 보다 높은 수준의 지반정보가 요구되고 있다. 그러나 우리나라에서는 최근까지도 비교적 결과의 신뢰도가 낮은 표준관입실험 등의 원위치 실험법에 의존하고 있는 현실이다. 본 논문에서는 복합적인 지반의 거동 특성 평가를 위하여 연약지반은 물론 암반에서도 에너지 변화를 측정할 수 있는 새로운 콘관입 실험기를 개발하고자 하였다. 본 연구에서는 롯드의 체결 방식에 따른 에너지 감쇠를 평가하기 위해 롯드를 인위적으로 불완전하게 체결하거나, 전체 길이는 일정하게 유지시키면서 체결된 롯드의 수를 변화시키며 실험을 수행하였다. 또한, 체결 조건이 다른 롯드에서 탄성파의 전달율과 반사율을 적용하여 에너지 손실을 평가 하였다. 그리고 정동적 콘관입 실험 장비를 제작하여 두부와 선단부에 변형률계와 가속도계를 부착함으로써 에너지 손실을 실내 실험을 통하여 평가하고자 하였다. 실험 결과 롯드의 체결 상태가 완벽하지 않으면 에너지 전달률이 급격히 감소하는 것을 실험과 시뮬레이션을 통하여 알 수 있었다. 또한 실내 실험결과 새로 개발된 정동적 콘 관입 실험기의 두부와 선단부의 전달에너지가 다름을 알 수 있었다. 본 연구에서는 두부는 물론 선단부에서의 에너지 평가가 필요함을 알 수 있었다.

• 콘크리트학회지
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• 제6권2호
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• pp.97-107
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• 1994

폴리머 침투콘크리트는 경화된 보통 콘크리트에 폴리머 침투제를 침투시켜 제조되는 신소재의 폴리머-콘크리트 복합체이다. 본 연구는 아크릴계 열가소성수지를 이용한 폴리머 침투콘크리트를 개발하고 폴리머 침투콘크리트 휨부재의 거동평가를 위한 재료모델, 구조해석 과정과 구조해석 프로그램을 개발하는데 목적이 있다. 본 연구는 크게 두 부분으로 구성된다. 첫 번째 단계에서는 결정성 고분자모노머인 methyl methacrylate(MMA)를 대상으로 침투성, 반응성, 열적 안정성 및 물성개선 효과를 종합적으로 분석하여 폴리머 침투제의 구성비와 제조공정을 정립하고, 본 연구의 실험자료로부터 폴리머 침투콘크리트의 제 강도특성, 파괴인성, 파괴에너지, 응력-변형률 관계 및 인장연화 관계를 보통 콘크리트의 압툭강도와 휨강도, 폴리머 함유율, 부재깊이, 초기 인공균열깊이 등의 함수로 각각 실험공식을 도출한다. 두 번째 단계에서는 MMA계 폴리머 침투콘크리트 구조부재의 하중단계별 탄성거동, 극한거동 및 인장연화거동을 해석하기 위한 구조해석 프로그램을 개발하고, 연구결과의 타당성과 적용성을 입증하기 위하여 폴리머 침투콘크리트의 제조공정, 제 실험공식 및 구조해석 프로그램은 실측거동을 잘 반영하고 있으므로 제한된 범위내에서 MMA계 폴리머 침투콘크리트 구조부재의 제조, 물성평가 및 거동해석에 적용 가능한 것으로 사료된다.

• Nuclear Engineering and Technology
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• 제53권9호
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• pp.3068-3084
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• 2021

Investigations of large commercial aircraft impact effect on nuclear power plant (NPP) buildings have been drawing extensive attentions, particularly after the 9/11 event, and this paper aims to experimentally assess the damage and vibrations of NPP buildings subjected to aircraft crash. In present Part I, two shots of reduce-scaled model test of aircraft impacting on NPP building were carried out. Firstly, the 1:15 aircraft model (weighs 135 kg) and RC NPP model (weighs about 70 t) are designed and prepared. Then, based on the large rocket sled loading test platform, the aircraft models were accelerated to impact perpendicularly on the two sides of NPP model, i.e., containment and auxiliary buildings, with a velocity of about 170 m/s. The strain-time histories of rebars within the impact area and acceleration-time histories of each floor of NPP model are derived from the pre-arranged twenty-one strain gauges and twenty tri-axial accelerometers, and the whole impact processes were recorded by three high-speed cameras. The local penetration and perforation failure modes occurred respectively in the collision scenarios of containment and auxiliary buildings, and some suggestions for the NPP design are given. The maximum acceleration in the 1:15 scaled tests is 1785.73 g, and thus the corresponding maximum resultant acceleration in a prototype impact might be about 119 g, which poses a potential threat to the nuclear equipment. Furthermore, it was found that the nonlinear decrease of vibrations along the height was well reflected by the variations of both the maximum resultant vibrations and Cumulative Absolute Velocity (CAV). The present experimental work on the damage and dynamic responses of NPP structure under aircraft impact is firstly presented, which could provide a benchmark basis for further safety assessments of prototype NPP structure as well as inner systems and components against aircraft crash.

• 한국지반공학회논문집
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• 제25권11호
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• pp.105-115
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• 2009

본 연구에서는 제주특별자치도 강정항 인근 해역에서 채취한 해사의 기본특성을 분석하고, 실내 챔버시험으로부터 미고결 제주해사의 콘선단저항-상대밀도-연직구속압의 관계를 결정하였다. 또한 현장 표준관입시험, 콘관입시험, Suspension-PS 검층을 수행하여 제주인근 해역 퇴적층의 고결가능성을 평가하였다. 시험결과, 제주해사는 입자의 각진 정도가 크고 입자표면과 내부에 공극이 발달하여 최대, 최소간극비가 큰 탄산염 모래의 특성을 가지고 있었으며, 큰 압축성을 가진 규산염 모래와 유사한 콘선단저항 분포를 나타내었다. 그러나 미고결 제주해사의 $q_c-D_R-{\sigma}_v'$ 관계는 제주인근 해역 퇴적층의 상대밀도를 과대평가하였다. 관입시험(CPT, SPT) 결과와 Suspension-PS 시험에 의한 미소변형전단탄성계수를 비교한 결과, 제주인근 해역의 퇴적층은 고결 가능성이 매우 높은 것으로 확인되었다.

• 한국축산식품학회지
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• 제35권2호
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• pp.211-215
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• 2015

From the previous study, Enterococcus faecium SH01 was isolated from mukeunji, an over-ripened kimchi, and it produced bacteriocin SH01. Bacteriocin SH01 showed an inhibitory effect against Listeria monocytogenes ATCC 19111, a bacterial strain causing human listeriosis. Crude bacteriocin SH01 was purified by ammonium sulfate precipitation and its inhibitory activity at two concentrations (500 and 1,000 AU/g) against Listeria monocytogenes ATCC 19111 was investigated in ground beef at increasing temperatures (5, 10, 15, and 20℃) for 8 d. The number of Listeria monocytogenes ATCC 19111 significantly decreased (p<0.05) as the concentration of bacteriocin increased from 500 to 1,000 AU/g. Intrinsic crude protease activities in ground beef were examined and increased as the temperature increased. Experiments varying both the concentrations of added bacteriocin SH01 and temperature demonstrated a maximum inhibition (2.33 log reduction of bacteria) in samples containing 1,000 AU/g of bacteriocin SH01 incubated at 20℃. When the crude bacteriocin SH01 solution (1,280 AU/mL) was incubated with crude protease solutions at different temperatures, its activity decreased by only half (640 AU/mL), as assessed in an agar well diffusion assay. The finding that the antilisterial activity of bacteriocin SH01 increased with temperature can be explained by the fact that higher temperatures increase bacterial membrane fluidity, thereby promoting the cellular penetration of bacteriocin SH01 into L. monocytogenes. Bacteriocin SH01 may be an excellent candidate as a biopreservative for controlling L. monocytogenes growth in ground beef.

• 한국농공학회지
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• 제38권5호
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• pp.140-154
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• 1996

In this study, displacement, deformation, and stability according to change of cohesion and internal friction angle were investigated through elasto-plastic method, finite-element method, and in-site experiment when excavating soft ground using sheet pile. The results of the study were as follows : 1. The horizontal displacement was 5.5% of the excavation depth by the elasto-plastic method and 3.9% of the excavation depth by the on-site experiment at the final excavation depth(GL-8.Om) on the condition of double stair strut after excavating GL-6.Om. 2. Relationships between cohesion(c) and internal friction angle $({\varphi})$ when safety factor to the penetration depth was 1.2 is shown in the following equations : (a) c= -O.0086$({\varphi})$+ O.3(D=3m) and (b) c=-0.00933$({\varphi})$+0.14(D=4m). 3. The results of elasto-plastic method and the experiment show that possible excavation depth was GL-6.Om after setting single stair strut in a short period in terms of possibility of carrying out on the condition of experimental site on the contrary general reinforcement method, setting double stair strut after excavating GL-4.0m. 4. After setting the strut, distribution of the horizontal displacement had concentrated on the excavation base and possible local failure which the shear strain caused decreased by the strut reinforced. 5. After setting strut, displacement of sheet pile was decreased by half, the limit of stable excavation depth of ground was GL-8.Om, and the maximum horizontal displacement at the GL-8.Om was 1.6% of excavation depth by the elasto-plastic method, 0.7% of excavation depth by the finite-element method.

• The Plant Pathology Journal
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• 제36권4호
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• pp.314-322
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• 2020

Interplay between histone acetylation and deacetylation is one of the key components in epigenetic regulation of transcription. Here we report the requirement of MoHDA1-mediated histone deacetylation during asexual development and pathogenesis for the rice blast fungus, Magnaporthe oryzae. Structural similarity and phylogenetic analysis suggested that MoHDA1 is an ortholog of Saccharomyces cerevisiae Hda1, which is a representative member of class II histone deacetylases. Targeted deletion of MoHDA1 caused a little decrease in radial growth and large reduction in asexual sporulation. Comparison of acetylation levels for H3K9 and H3K14 showed that lack of MoHDA1 gene led to significant increase in H3K9 and H3K14 acetylation level, compared to the wild-type and complementation strain, confirming that it is a bona fide histone deacetylase. Expression analysis on some of the key genes involved in asexual reproduction under sporulation-promoting condition showed almost no differences among strains, except for MoCON6 gene, which was up-regulated more than 6-fold in the mutant than wild-type. Although the deletion mutant displayed little defects in germination and subsequent appressorium formation, the mutant was compromised in its ability to cause disease. Wound-inoculation showed that the mutant is impaired in invasive growth as well. We found that the mutant was defective in appressorium-mediated penetration of host, but did not lose the ability to grow on the media containing H₂O₂. Taken together, our data suggest that MoHDA1-dependent histone deacetylation is important for efficient asexual development and infection of host plants in M. oryzae.

• 미생물학회지
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• 제38권4호
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• pp.270-270
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• 2002

Pathogenic Salmonella typhimurium can invade the intestinal epithelium and cause a wide range of diseases including gastroenteritis and bacteremia in human and animals. To identify the genes involved in the infection, the invasion determinant was obtained from S. typhimurium 82/6915 and was subcloned into pGEM-7Z. A subclone DHl (pSV6235) invaded HEp-2 and Chinese hamster ovary epithelial cells and contained a 4.4 kb fragment of S. typhimurium genomic region. Compared with the host strain E. coli DHl, the subclone DHl (pSV6235) invaded cultured HEp-2 and Chinese hamster ovary cells at least 75- and 68-fold higher, respectively. The invasion rate of E. coli DHl for the cells significantly increased by harbouring the genomic region derived from pathogenic S. typhimurium 82/6915.

• Journal of Ginseng Research
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• 제38권3호
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• pp.215-219
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• 2014

Background: Biological control of plant pathogens using benign or beneficial microorganisms as antagonistic agents is currently considered to be an important component of integrated pest management in agricultural crops. In this study, we evaluated the potential of Bacillus subtilis strain HK-CSM-1 as a biological control agent against Colletotrichum panacicola. Methods: The potential of B. subtilis HK-CSM-1 as a biological control agent for ginseng anthracnose was assessed. C. panacicola was inoculated to ginseng plants and the incidence and severity of disease was assessed to examine the efficacy of the bacterium as a biological control against C. panacicola. Results: Inoculation of Panax ginseng plants with B. subtilis significantly suppressed the number of disease lesions of C. panacicola and was as effective as the chemical fungicide iminoctadine tris(albesilate). The antifungal activity of B. subtilis against C. panacicola was observed on a co-culture medium. Interestingly, treatment with B. subtilis did not significantly affect the diameter of the lesions, suggesting that the mechanism of protection was through the reduction in the incidence of infection related to the initial events of the infection cycle, including penetration and infection via spore germination and appressorium formation rather than by the inhibition of invasive growth after infection. Conclusion: Our results suggest that B. subtilis HK-CSM-1 can be used as an effective and ecologically friendly biological control agent for anthracnose in P. ginseng.