• 한국미생물·생명공학회지
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• 제30권3호
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• pp.216-222
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• 2002

고농도의 ethanol을 갖는 청주를 생산하기 위하여 일본양조협회 Saccharomyces cerevisiae 10호 균주로부터 ethanol 내성 균주 Saccharemyces cerevisiae SE211균주를 분리하였다. 분리 균주는 10%(v/v) ethanol이 포함되어 있는 배지에서의 성장속도가 빨랐으며, 높은 ethanol 농도에서도 성장이 가능하였다 또한 ethanol내성, 내삼투압성, 내열성 등이 원균주에 비해 우수하였다. 선발균주의 세포막 성분을 분석한 결과 sterol, phospholipid의 함량은 원균주와 차이가 없었으나, 지방산의 조성비는 차이를 보였다 또한 분리균주는 외부자극에 대한 저항성을 나타내는 물질로 알려진 세포내 trehalose의 함량이 원균주에 비해 증가하였다 따라서 선발균주의 ethanol내성 및 ethanol배지에서의 높은 성장속도는 세포막의 fatty acid및 세포내 trehalose함량을 변화시키는 능력이 높은 것에 기인한다고 생각되었다.

• 한국지반공학회논문집
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• 제16권4호
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• pp.95-105
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• 2000

공중낙하법에 의해 만든 등방압밀 모래공시체를 미소변형률 측정장치를 사용한 평면변형률압축시험을 실시하여 미소변형률에서 파괴후까지의 전단강성계수에 대한 이방성을 연구하였다. 세계각국의 주요 연구기관에서 사용되고 있는 7종류의 연구용 표준사 공시체를 멤브레인의 관입에 의한 오차와 변위를 외부에서 측정함으로 하여 생기는 오차(bedding error)등의 영향을 제거하여 측정한 최대주응력방향의 변형률과 최소주응력방향의 변형률을 각각 0.0001%에서 10%까지 넓은 범위에 걸친 응력-변형률 관계를 얻었다. 그 결과 최대전단강성계수 Gmax는 퇴적면과 최대주응력 $\sigma$1이 이루는 각도 $\delta$에 관계없이 일정하였다. 그러나 정규화한 Gmax는 모래의 종류에 따라 달랐다. 또 전단강성계수의 변형률 수준과 응력 수준에 대한 의존성은 $\delta$가 감소함에 따라 증가하였다.

• 한국지반공학회지:지반
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• 제14권4호
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• pp.33-46
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• 1998

공중낙하법에 의해 만든 등방압밀 모래공시 체를 미소변형률 측정장치를 사용한 평면변형률압축 시험을 실시하여 미소변형률에서 파괴 후까지의 강성률에 대한 이방성을 연구하였다. 세계 각국의 주요 연구기관에서 사용되고 있는 7종류의 연구용 표준사 공시체를 멤브레인의 관입에 의한 오차와 변위를 외부에서 측정함으로 하여 생기는 오차(bedding error) 등의 영향을 제거하여 측정한 최대주응력방향의 변형률과 최소주응력방향의 변형률을 각각 0.0001%에서 10%까지 넓은 범위에 걸친 응력-변형률 관계를 얻었다. 그 결과 최대 영률 $E_{max}$은 퇴적면과 최대주응력 $\delta_1$이 이루는 각도에 관계없이 일정하였다. 그러나, 정규화한$E_{max}$은 모래의 종류에 따라 달랐다. 또, 강성률의 변형률 수준과 응력 수준에 대한 의존성은 $\delta$가 감소함에 따라 증가하였다.

• 대한금속재료학회지
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• 제56권11호
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• pp.835-843
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• 2018

In this work, we report a delta rosette strain sensor based on highly stretchable silver nanowire (AgNW) percolation piezoresistors. The proposed rosette strain sensors were easily prepared by a facile two-step fabrication route. First, three identical AgNW piezoresistive electrodes were patterned in a simple and precise manner on a donor film using a solution-processed drop-coating of the AgNWs in conjunction with a tape-type shadow mask. The patterned AgNW electrodes were then entirely transferred to an elastomeric substrate while embedding them in the polymer matrix. The fabricated stretchable AgNW piezoresistors could be operated at up to 20% strain without electrical or mechanical failure, showing a maximum gauge factor as high as 5.3, low hysteresis, and high linearity ($r^2{\approx}0.996$). Moreover, the sensor responses were also found to be highly stable and reversible even under repeated strain loading/unloading for up to 1000 cycles at a maximum tensile strain of 20%, mainly due to the mechanical stability of the AgNW/elastomer composites. In addition, both the magnitude and direction of the principal strain could be precisely characterized by configuring three identical AgNW piezoresistors in a delta rosette form, representing the potential for employing the devices as a multidimensional strain sensor in various practical applications.

• Journal of Microbiology and Biotechnology
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• 제32권11호
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• pp.1485-1495
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• 2022

The development of a yeast strain capable of fermenting mixed sugars efficiently is crucial for producing biofuels and value-added materials from cellulosic biomass. Previously, a mutant Pichia stipitis YN14 strain capable of co-fermenting xylose and cellobiose was developed through evolutionary engineering of the wild-type P. stipitis CBS6054 strain, which was incapable of co-fermenting xylose and cellobiose. In this study, through genomic and transcriptomic analyses, we sought to investigate the reasons for the improved sugar metabolic performance of the mutant YN14 strain in comparison with the parental CBS6054 strain. Unfortunately, comparative whole-genome sequencing (WGS) showed no mutation in any of the genes involved in the cellobiose metabolism between the two strains. However, comparative RNA sequencing (RNA-seq) revealed that the YN14 strain had 101.2 times and 5.9 times higher expression levels of HXT2.3 and BGL2 genes involved in cellobiose metabolism, and 6.9 times and 75.9 times lower expression levels of COX17 and SOD2.2 genes involved in respiration, respectively, compared with the CBS6054 strain. This may explain how the YN14 strain enhanced cellobiose metabolic performance and shifted the direction of cellobiose metabolic flux from respiration to fermentation in the presence of cellobiose compared with the CBS6054 strain.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2008년도 추계학술대회 논문집
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• pp.95-98
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• 2008

In the present work, cross-roil rolling was rallied out using a rolling mill in which the roll axes are tilted by $5^{\circ},\;7.5^{\circ},\;10^{\circ}$ towards the transverse direction of the roiled sample. The evolution of strain states during cross-roll rolling was investigated by three-dimensional finite element method (FEM) simulation. Parallel to cross-roll rolling, normal-rolling using a conventional rolling mill was also carried out in the same rolling condition for clarifying the effect of cross-roll rolling. It turned out that three shear rate components were all introduced to the rolled sample by the cross-roll rolling process, while only one shear rate component operated during normal-rolling.

• 소성∙가공
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• 제12권1호
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• pp.43-48
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• 2003

The strain state during the continuous confined strip shearing (CCSS) based on ECAP was tackled by means of a two-dimensional FEM analysis. The deformation of AA 1100 sheet in the CCSS apparatus was composed of three distinct processes of rolling, bending and shearing. The pronounced difference in the friction conditions on the upper and lower roll surfaces led to the different variation of the strain component ${epsilon}_13$ throughout the thickness of the aluminum sheet. Strain accompanying bending was negligible because of a large radius of curvature. The shear deformation was concentrated at the corner of the CCSSchannel where the abrupt change in the direction of material flow occurred. The process variables involving the CCSS-die design and frictions between tools and strip influenced the evolution of shear strains during CCSS.

• 대한기계학회논문집A
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• 제32권3호
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• pp.250-257
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• 2008

We calculate the variation of the surface stresses according to uniaxial and biaxial strains in face-centered cubic (FCC) metals. In our study, three mainly observed free surfaces of seven representative FCC metals are considered. Employed method is molecular mechanics, in which the interaction of atoms is described by empirical interatomic potentials. As uniaxial strain increases to tensile direction, the surface stresses on {100} and {110} free surfaces decrease monotonously, while those on {111} surface increase. These tendencies are the same regardless of the species of metals and interatomic potentials employed. However, when the system is under biaxial strain, surface stresses change different according to the surface directions, the species of metals, and even interatomic potentials. On {100} and {111} surfaces, heavy metals (Pt, Au) show the opposite variation to light metals (Ni, Cu). In the cases of Pd and Ag, the surface stresses reveal the opposite tendency, depending on interatomic potentials used.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2007년도 춘계학술대회논문집
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• pp.934-937
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• 2007

This study focused on investigating the effect of aligned cellulose fibers to the performance of EAPap actuator. The performance of EAPap is dependant on the material direction of cellulose film. Electrospinning was used to improve material directionality of EAPap. DMAc cellulose solution which cotton pulp was resolved in DMAc solvent was used for electrospinning cellulose film. To increase directionality of nano fibers, the Electrospun film was stretched by 10 % strain during drying process. Induced in-plane strain of Electrospun EAPap was proportional to the applied voltage and larger than that of spincast EAPap. It is concluded that the performance of EAPap was improved by aligning cellulose fibers.