• Title/Summary/Keyword: strain concentration

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Experimental Studies of The Effects of Kyungok-go against Mycobacteria tuberculosis (경옥고(瓊玉膏)가 결핵균(結核菌)에 미치는 영향(影響)에 관(關)한 실험적(實驗的) 연구(硏究))

  • Jung, Hee-Jae;Jung, Seung-Ki;Rhee, Hyung-Koo;Jeon, Sang-Bok
    • The Journal of Internal Korean Medicine
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    • v.21 no.4
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    • pp.555-563
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    • 2000
  • Objective : In order to know the antibacterial effects of Kyungok-go against Mycobacteria tuberculosis, Methods : In this study, I inverstigated these effects in terms of combination of other antibiotics with and without Kyungok-go on several different media conditions. Results: On Ogawa medium, Kyungok-go of the $10{\mu}/ml$ concentration showed the anti-Mycobacteria tuberculosis activity against antibiotic drug-sensitive strain. On Middle-blue medium, Kyungok-go of the $10{\mu}/ml$ concentration showed the anti-Mycobacteria tuberculosis activity against antibiotic drug-sensitive strain. Kyungok-go showed the anti mycobacteria tuberculosis activity with the meaningful result above a certain concentration. The resistance against M, tuberculosis as the concentration of Kyungok-go was decreased significantlly on the high concentration($500{\mu}/ml$) When rifampicin and Kyungok-go were used together, the resistance was decreased with the statistical significance as to the persistant antibacterial effect against M. tuberculosis, When ciprofloxacin and Kyungok-go were used together, the resistance was decreased with the statistical significance as to the persistant antibacterial effect against M. tuberculosis, The combination of treatment, Kyungok-go with both rifampicin and ciprofloxacin, showed much better antibacterial effect against M, tuberculosis than antibiotics alone. Conclusions : This study shows that Kyungok-go has antibacterial effect against M. tuberculosis and in the combination of treatment, Kyungok-go with antibiotics, showed much better antibacterial effect against M. tuberculosis than antibiotics alone,.

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Extinguishing Concentration of Inert Gases in Heptane Pool Fires (헵탄 풀화재에서 불활성기체 소화농도)

  • Jung, Tae-Hee;Lee, Eui-Ju
    • Journal of the Korean Society of Safety
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    • v.27 no.3
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    • pp.71-76
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    • 2012
  • The coflow velocity effect on the minimum extinguishing concentration(MEC) was investigated experimentally in heptane cup-burner flames. Various inert gases($N_2$, Ar, $CO_2$, He) were added into the oxidizer to find the critical concentration and the effectiveness of the agents on flame extinction. The experimental results showed that the MECs were increased with increasing coflow velocity for most inert gases except helium, but the higher coflow velocity induced the lower burning rates of heptane. This indicated that the increase of coflow velocity resulted in the decrease of fuel velocity evaporated from fuel surface, and hence the stain rate on the reaction zone was also decreased. In the case of helium as a additive, the extinguishing concentration was independent of the coflow velocity because the heat conductivity was ten times larger than the other inert gases and flow effect by a strain rate might be compensated for heat loss to the surroundings.

Characterization of a potential probiotic strain Lactobacillus plantarum MY4

  • Jeong, Hwang-Yeong;Jeong, Min-Yong;Kim, Gi-Tae;Kim, Cheon-Je;Baek, Hyeon-Dong
    • 한국생물공학회:학술대회논문집
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    • 2001.11a
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    • pp.882-885
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    • 2001
  • The cells of Lactobacillus plantarum MY4 isolated from the human feces were treated for 24 h in artificial bile after incubation for 2 h in artificial gastric juice and final number of the strain was reached to around $3.1{\times}10^8$ CFU/ml. In test of API ZYM kit, ${\beta}$-glucuronidase or ${\beta}$-glucosidase were not produced by L. plantarum MY4. However, ${\beta}$-galactosidase were weakly produced by it, which they would be alleviated the lactose intolerance. L. plantarum MY4 were resistant to antibiotics such as nisin, tetracycline, streptomycin, rifamycin, doxycycline, roxithromycin, chloramphenicol, nystatin, erythromycin, ciprofloxacin and gentamycin. L. plantarum MY4 was affected by alcohol concentration up to 8%, but more than 16%, their growth was not affected significantly. L. plantarum MY4 was shown to inhibit the growth of Listeria monocytogenes ATCC 19111 completely within 24 h of incubation, which indicates its bactericidal nature. Thus, L plantarum MY4 show promise as a probiotic strain because of its characteristics.

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Pure Isolation, Identification and Culture Characteristics of Ethylene Glycol Degrading Bacteria (Ethylene Glycol 분해균주의 순수분리.동전 및 배양특성)

  • 류원률;최장승;주무환
    • KSBB Journal
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    • v.13 no.5
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    • pp.518-523
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    • 1998
  • A large quantity of ethylene glycol(EG) is remained in the effluent after pretreating polyester weight-loss wastewater physicochemically in the fist stage and must be treated biologically in the second stage. Therefore, an excellent EG-utilizing bacteria strain was isolated from the natural system and the optimal culture conditions of the strain were investigated. The optimal culture conditions of temperature, pH, and nitrogen source were found to be 35$^{\circ}C$, 7.5 and ammonium chloride, respectively, when CODCr removal efficiency was more than 90%. The growth of stains and EG removal efficiency was slightly improved by adding elements such as niacin and biotin. With increasing inoculation size in a batch culture, the removal efficiency of EG was conspicuously increased. Growth rate was inhibited when the initial concentration of EG was more then 30g/L. The strain was identified as Pseudomonas sp. based on morphological and biological characteristics and named as Pseudomonas sp. EG1.

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Mode Sensing of a Composite Beam Using Fiber Bragg Grating Sensor (광섬유 브래그 격자 센서를 이용한 구조물의 모드 형상 측정)

  • 구본용;류치영;홍창선
    • Proceedings of the Korean Society For Composite Materials Conference
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    • 2000.04a
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    • pp.163-166
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    • 2000
  • Fiber Bra99 grating (FBG) sensor, one of the fiber optic sensor (FOS) offers lots of advantages for structural health monitoring due to its multiplexing capability. Also, it is proper to measure the structural vibration with no mass concentration effect. In this paper, we constructed two sensor arrays composed of 9 FBG sensors for the vibration and mode sensing of a composites beam. For an accurate measurement of wavelength shift, a signal processing board with an electric circuit based on time-interval counting was developed. This sensor system showed a good resolution of dynamic strain (<10${\mu}{\varepsilon}$). Using this sensor system, dynamic strains at 9 points of composite beam was measured and strain measured mode shape of the beam was calculated from the acquired strains and compared with numerical results by ABAQUS.

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Use of High Order Vibration Modes for Design of Piezo Energy Harvester (압전 발전기의 설계를 위한 고차 모드의 활용)

  • Hwang, Woo-Seok
    • Transactions of the Korean Society for Noise and Vibration Engineering
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    • v.22 no.4
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    • pp.372-376
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    • 2012
  • The most common type of the piezoelectric energy harvester is the cantilevered beam since it is attached to the host structure and tuned to the frequency of the base excitation easily. However, the excessive strain at the fixed end of cantilevered beam causes some problems such as fatigue and durability. The use of higher vibration modes of the cantilevered beam may reduce the concentration of the strain at the fixed end since the strains of higher modes are distributed along the span. The results show that the use of high vibration mode is not efficient for power generation, but it reduces the excessive strain lever at typical region to prevent the failure by fatigue.

Effects of Environmental Factors on Degradation of Aroclors by Gram-negative Bacteria (Gram 음성세균에 의한 Aroclor 분해에 미치는 환경요소의 영향)

  • 김치경;김문식
    • Korean Journal of Microbiology
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    • v.28 no.2
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    • pp.145-150
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    • 1990
  • The effects of environmental factors on degradation of Aroclor 1242 were investigated with four Gram-negative bacterial isolates. Their biodegradabilities of the Aroclor were well correlated to their growth rates on the Aroclor added as a sole carbon and energy source. The optimum concentration of the Aroclor for biodegradation of the substrate in MM2 medium was 0.5mg/ml in HK-100, HK-123, and MS-1003 strains, but 1 mg/ml in DJ-26 strain. The optimum temperature and pH were $30^{\circ}C$ and 7.0, respectively, for all the strains. On the basis of the results which the strain of DJ-26 showed the highest degradability of the Aroclor as well as the highest growth rate under the optimum environmental conditions, the bacterial isolate identified as Pseudomonas sp. was found to be a strain usable for treatment of the toxic and recalcitrant chemical pollutants, such as polychlorinated aromatic hydrocarbons.

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Enhanced Alcohol Production from Synthesis Gas Using a CO-resistant Mutant of Clostridium sp. AWRP (CO 내성을 갖는 Clostridium sp. AWRP 돌연변이주의 분리 및 이를 이용한 합성가스로부터의 에탄올 생산 연구)

  • Kwon, Soo Jae;Lee, Joungmin;Lee, Hyun Sook
    • Microbiology and Biotechnology Letters
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    • v.47 no.4
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    • pp.581-584
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    • 2019
  • In this study, the carbon monoxide (CO)-fermenting acetogen, Clostridium sp. AWRP was subjected to chemical mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine (NTG) to generate a CO-resistant mutant. Among the 26 colonies obtained, the highest alcohol production was observed in one isolate, named C1. Compared to the wild-type strain, the C1 strain exhibited 1.5- and 3.4-fold higher CO consumption rate and alcohol selectivity, respectively. The total CO consumption of strain C1 could be further enhanced by increasing the content of metal ions, such as nickel and iron. The highest ethanol titer (5.7 g/l) was achieved by 5-fold increase in the iron concentration.

Separation of ε-poly-L-lysine from the fermentation broth of Streptomyces albulus (Streptomyces albulus 배양액으로부터 ε-poly-L-lysine의 분리)

  • Sun, Heung-Suk;Park, Chan-Young
    • Journal of the Korean Society of Industry Convergence
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    • v.2 no.1
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    • pp.77-83
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    • 1999
  • Grown in the secondary broth of production media, the strain Streptomyces albulus has increased more the production of its metabolite ${\varepsilon}$-poly-L-lysine, one of poly(amino acid)s used as disinfecting food additives, than the strain in the primary culture of growth nutrients. Having the strain removed, the large concentrate obtained by ultrafiltrating the secondary culture broth. The concentrated production broth exchanged into followed by detecting in UV flowcell at 220nm the peptide bond of the components eluting the adsorbed proteins and polylysine with NaCl salt of gradient concentration, and has separated into five components. Among them the component in the fourth peak fraction has proved to be the pure ${\varepsilon}$-poly-L-lysine after the portion being hydrolyzed the fraction with HCl into amino acid followed by being the composing amino acid analysis.

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Isolation and Characterization of a Cephalosporin C Resistant and 7-Aminocephalosporanic Acid Sensitive Strain (Cephalosporin C 내성과 7-Aminocephalosporanic Acid 감수성을 지닌 균주의 선발 및 특성)

  • Kim, Ook-Hyun;Park, Yong-Chjun;Lim, Jai-Yun;Kim, Young-Chang
    • Microbiology and Biotechnology Letters
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    • v.23 no.5
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    • pp.556-558
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    • 1995
  • A strain which showed cephalosporin C resistance and 7-aminocephalosporanic acid sensitivity was isolated from nature. Among the isolates, SS5 was sensitive to cephalosporin C, penicillin G, ampicillin, 7-aminocephalosporanic acid, 6-aminopenicillanic acid, and 7-aminodeacetoxy cephatosporanic acid at concentrations of 1,000 $\mu $g/ml, 2,000 $\mu $g/ml, 3,000 $\mu $g/ml, 30 $\mu $g/ml 100 $\mu $g/ml and 100 $\mu $g/ml, respectively. But SS5 was sensitive at very low concentration of chloramphenicol, kanamycin, neomycin, streptomycin and tetracycline. Since SS5 was sensitive to 7-ACA (30 $\mu $g/ml) and didn't have $\beta $-lactamase activity on the cephalosporin C, SS5 could be useful as an indicator strain for the production of 7-ACA, which is an important precursor for the synthesis of many semisynthetic cephalosporins.

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