• 제목/요약/키워드: strain 320

검색결과 85건 처리시간 0.036초

Effect of Cyclic AMP on the Two Promoters of Escherichia coli Thioredoxin Gene

  • Sa, Jae-Hoon;Fuchs, James A.;Lim, Chang-Jin
    • BMB Reports
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    • 제30권5호
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    • pp.320-325
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    • 1997
  • Thioredoxin is a multi-functional protein which is ubiquitous in microorganisms, animals and plants. Previously, expression of the E. coli thioredoxin gene was found to be negatively regulated by cAMP. In the present study, the effect of cAMP on two separate promoters of the E. coli thioredoxin gene was investigated. Cyclic AMP had a repressible effect on P1 and P1P2 promoter activity of the constructs. This effect was also observed in the cya strain. The P2 promoter construct gave very high -galactosidase activity, and its expression was not affected by exogenous cAMP. It was assumed that a cis-acting negative element, probably the cAMP-CRP binding site, might have been deleted in the P1 promoter construct. Repression of the thioredoxin gene expression by cAMP appeared to be independent of ppGpp.

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The Effects of Natto Mucilage on Serum Nitrogen Compounds in Rats (낫도(Natto) 점질물이 흰쥐의 혈청 질소화합물에 미치는 영향)

  • Kim, Song-Chon;Kim, Man-Soo
    • Journal of the Korean Applied Science and Technology
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    • 제19권4호
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    • pp.320-326
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    • 2002
  • Natto, one of Japanese traditional food is made from steamed whole soybeans fermented with Bacillus natto. In this study, the effects of Natto mucilage- feeding on griwth, organ weight and serum nitrogen compounds of rats were investigated. Male rats in Sprague-Dawley strain were fed on basal diets supplemented with aqueous Natto mucilage of several percentages for 10 weeks. Natto mucilage had no significant effects on the body and organ weights. The levels of GOT, GPT, LDH. and total bilirubin in serum of rats fed Natto mucilage diets were significantly lower than those in the control group ' The levels of total protein and albumin were higher than those in the control group. The level of calcium in serum indicates a growing tendency. but creatinine, uric acid, and BUN had declining. And the most component of free am1no acids in Natto mucilage were glutamin acid.

Estimation of displacement responses of a suspension bridge by using mode decomposition technique (모드분해기법을 이용한 현수교의 변위응답추정)

  • Chang, Sung-Jin;Kim, Nam-Sik;Kim, Ho-Kyung
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 한국소음진동공학회 2009년도 춘계학술대회 논문집
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    • pp.320-325
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    • 2009
  • In this study, a method to estimate the suspension bridge deflection is developed using mode decomposition technique. In order to examine the suspension bridge stability against these dynamic loadings, the prediction of displacement response is very important to evaluate bridge stability. However, it is recognized that any measurement of movement for suspension bridges may be difficult for the absence of proper methods to measure the displacement response on site. This study aims at suggesting a method to estimate the displacement response from the measured strain signals in an indirect way to predict the displacement response, not a direct way to measure the displacement response. Additionally, by applying the FBG sensors with multi-point measurements not influenced by electric noise, it can be expected that the technique would be applicable to infrastructures.

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Experimental Study on Failure Behavior of Plain Concrete - Biaxial Stress Test (콘크리트 파괴거동특성의 실험적 연구I-이축응력시험)

  • 이상근;이상민;박상순;한상훈;송영철
    • Proceedings of the Korea Concrete Institute Conference
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    • 한국콘크리트학회 2003년도 봄 학술발표회 논문집
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    • pp.315-320
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    • 2003
  • Two different strength types of plain concrete plate specimens (200$\times$200$\times$60mm) were tested under different biaxial load combinations. The specimens were subjected to biaxial combinations covering the three regions of compression-compression, compression-tension, and tension-tension. The loading platens with Teflon pads were used to reduce a confining effect in boundary surface between the concrete specimen and the solid platen. The principal deformations in the specimens were recorded, and the failure modes along with each stress ratio were examined. Based on the strength data, the failure envelops were developed for each type of plain concrete. The biaxial stress-strain responses of concrete plate specimens for three biaxial loading regions were also plotted. The test data indicated that the strength of concrete under biaxial compression ($f_2 / f_1$$_1$=-1/-1) is about 17 percent larger than under uniaxial compression.

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Development of Austempered Ductile Iron With High Strength and High Toughness for Automotive Parts (고강도 ADI 의 자동차 부품개발에 관한 연구)

  • Kim, Won-Yong;Lee, Young-Sang;Kim, Gwang-Bae;Kang, In-Chan
    • Journal of Korea Foundry Society
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    • 제10권5호
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    • pp.408-416
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    • 1990
  • The application of this new design approach called fracture mechanics allow one to determine the maximum allowable stress from the knowledge of the largest expected flow size and the plane strain fracture toughness of a material. In this study we examined the relation between retained austenite, mechanical property and fracture toughness accompanied by austempering heat treatment. Fracture toughness values and retained austenite volume were higher with the ADI(austempered ductile iron) which were austempered at $380^{\circ}C$ than austempered at $320^{\circ}C$. Additionally, fracture toughness values were increased for 1~2 hour austempering time but it was slowly decreased for 5 hour ADI maintaining the predominant fracture toughness($K_{IC}:83MPa{\sqrt{m}}$) is obtained following condition, namely, austempering temperature and time ($380^{\circ}C$ and 1 hour).

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Analysis and Identification of Bacteria with Antifungal Activity

  • Kim, Seon-A;Chio, Hey-Jung;Lee, Young-Guen;Choi, Yong-Lark;Jeong, Yong-Kee;Lee, Hye-Rin;Kim, Dong-Wan;Moon, Ja-Young;Joo, Woo-Hong
    • Proceedings of the Korean Society of Life Science Conference
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    • 한국생명과학회 2007년도 제48회 학술심포지움 및 추계국제학술대회
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    • pp.74.2-74.2
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    • 2007
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Tension and impact behaviors of new type fiber reinforced concrete

  • Deng, Zongcai;Li, Jianhui
    • Computers and Concrete
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    • 제4권1호
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    • pp.19-32
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    • 2007
  • This paper is concentrated on the behaviors of five different types of fiber reinforced concrete (FRC) in uniaxial tension and flexural impact. The complete stress-strain responses in tension were acquired through a systematic experimental program. It was found that the tensile peak strains of concrete with micro polyethylene (PEF) fiber are about 18-31% higher than that of matrix concrete, those for composite with macro polypropylene fiber is 40-83% higher than that of steel fiber reinforced concrete (SFRC). The fracture energy of composites with micro-fiber is 23-67% higher than that of matrix concrete; this for macro polypropylene fiber and steel fiber FRCs are about 150-210% and 270-320% larger than that of plain concrete respectively. Micro-fiber is more effective than macro-fiber for initial crack impact resistance; however, the failure impact resistance of macro-fiber is significantly larger than that of microfiber, especially macro-polypropylene-fiber.

Threonine Production by A Newly Isolated and Characterized Methylotrophic Bacterium (새로운 메탄올자화세균에 의한 트레오닌의 생산)

  • Kim, Kyoung-Ja;Park, Kui-Lea
    • YAKHAK HOEJI
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    • 제36권4호
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    • pp.315-320
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    • 1992
  • The amino acid threonine was produced from glycine and ethanol in a reaction mixture using resting cells of a newly isolated gram-negative methylotrophic bacterium, capable of growth on methanol. The isolate could utilize $C_1$ compounds and a variety of multicarbon substrates as sole carbon and energy source. To obtain cells of isolate with high threonine producing activity, we investigated optimum cultural conditions. Optimal growth was at the initial concentration of 0.5%(v/v) methanol, at $30^{\circ}C$ and pH 7.0. The growth was not affected by antibiotics inhibiting cell wall synthesis, but was completely suppressed by those inhibiting protein synthesis. The optimum reaction conditions from threonine production by resting cells of this strain were found.

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Isolation of Brucella Suis from Aborted Fetus of a Dog (유산견(流産犬) 태아(胎兒)로부터 Brucella suis의 분리(分離))

  • Tak, Ryun-Bin;Chun, Do-Ki
    • The Journal of the Korean Society for Microbiology
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    • 제7권1호
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    • pp.17-20
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    • 1972
  • A strain of Brucella showing the characteristics of Br. suis was isolated from a 55 day-old aborated fetus of a German Shepherd and the cause of the abortion was considered due to brucellosis. A considerable number of dog sera collected at the slaughter house in Taegu city showed antibody titers of 1:80 or more to Br. suis, and serum from the aborted dog 1:320 to Br. suis in titer.

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Epidemiology of Soybean Mosaic Virus Diseases (콩모자이크바이러스병의 역학적 연구)

  • Cho Eui-Kyoo;Choi S.H.;Hwang C.Y.
    • Korean journal of applied entomology
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    • 제23권4호
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    • pp.197-202
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    • 1984
  • Two soybean cultivars Hanan and Kwanggyo were used to monitor SMV spread in the field. The Haman producing mosaic symptoms by infection with SMV strain G7 was planted in $2.2m^2$ at the coater of $320.7m^2$ field and Kwanggyo producing necrosis by infection with SMV-G7 was planted around the Haman one day after inoculation of Heman with SMV-G7. The most severe incidence of the necrotic disease in soybean cultivar Kwanggyo occurred on 43 days after planting(July 13) whereas aphid population reached peak around 22 days after planting(June 22). Total incidence of the necrotic disease was $20.4\%$, in the whole field and $56\%$, in eight $mall plots around the spreader of SMV. The spread of S MV outward from the spreader source was greater downwind than it was upwind. Spread also showed a signigicant gradieut pattern leeward from the infection focus.

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