• 한국건설순환자원학회논문집
• /
• 제10권4호
• /
• pp.395-401
• /
• 2022

이 연구에서는 프리텐션 기법으로 제작되는 중공 슬래브(hollow core slab, HCS) 부재 3종(H200, H320, H400)에 대하여 강연선의 전달길이를 평가하고 기존 설계식과 비교하였다. HCS 부재의 측면에 일정한 간격을 두어 변형률 게이지를 부착하였으며, 절단 과정에서 발생하는 변형률을 측정하였다. 변형률은 절단면에서 0이며, 부재의 중앙으로 갈수록 점차 증가하여 전달길이 위치에서 일정한 값을 보인다. 강연선이 한 가닥씩 배치되는 H200의 경우 대부분 설계식 범위 내(최대 762 mm)에 전달길이가 형성되나, 강연선이 세 가닥 배치되는 H320과 H400의 전달길이는 설계 범위보다 높은 수준(850 mm 이상)으로 나타났다.

• 한국소성가공학회:학술대회논문집
• /
• 한국소성가공학회 1999년도 제3회 압연심포지엄 논문집 압연기술의 미래개척 (Exploitation of Future Rolling Technologies)
• /
• pp.320-326
• /
• 1999

In the present investigation, recrystallization occurring during hot rolling of thick steel plate was predicted. The thermo-mechanical history of a material point was traced by the finite element method and the recrystallization was predicted by the Sellars equations. The investigation was performed for 4 different cases; two different pass schedules in conventional rolling and two different pass schedules in controller rolling. Variations of temperature, strain, strain rate and grain size were compared with each other. It was found out that the difference of grain size through thickness was more distinctive in the cases of controller rolling.

• 소성∙가공
• /
• 제7권2호
• /
• pp.158-167
• /
• 1998

Hot restoration mechanism flow stress and stain of the Al2024 composites reinforced with 1,8,15,36, and $44{\mu}m\;SiC_p$(10 vol. %) were studied by hot torsion tests. The hot restoration mechanism of all the composites was found to be dynamic recrystallization(DRX) at $320^{\circ}C$ while that of the composites reinforced with 1 and $8{\mu}m\;SiC_p$ was found to be dynamic recovery(DRX) at $480^{\circ}C$. It was found that the Al2024 composite with $15{\mu}m\;SiC_p$ showed the highest flow stress(${\sim}$223 MPa) at $320^{\circ}C$ under a strain rate of 1.0/sec. Also the highest flow strain of the composites was obtained at $430^{\circ}C$. The com-posites reinforced with 1 and $8{\mu}m\;SiC_p$ showed lower flow stress and higher flow strain at $480^{\circ}C$ than those of the composites reinforced with 15, 36, and $44\;{\mu}m\;SiC_p$ These result were discussed in relation to the transition of the hot restoration mechanism. $DRX{\leftrightarrow}DRV$. The dependence of flow stress on strain rate and temperature was attempted to fit with the hyperbolic sine equation ($\dot{\varepsilon}=A[sinh({\alpha}{\cdot}{\sigma}_p]^n$ exp(-Q/RT)and Zener-Hollomon parameter($Z=\;\dot{\varepsilon}\;exp(Q/RT))$.

• Mycobiology
• /
• 제37권4호
• /
• pp.267-271
• /
• 2009

A fungal strain producing a high level of cellulase was selected from 320 fungal isolates and identified as Aspergillus sp. This strain was further improved for cellulase production by sequential treatments by two repeated rounds of $\gamma$-irradiation of $Co^{60}$, ultraviolet treatment and four repeated rounds of treatment with N-methyl-N'-nitro-N-nitrosoguanidine. The best mutant strain, Aspergillus sp. XTG-4, was selected after screening and the activities of carboxymethyl cellulase, filter paper cellulase and $\beta$-glucosidase of the cellulase were improved by 2.03-, 3.20-, and 1.80-fold, respectively, when compared to the wild type strain. After being subcultured 19 times, the enzyme production of the mutant Aspergillus sp. XTG-4s was stable.

• Journal of Microbiology and Biotechnology
• /
• 제6권5호
• /
• pp.315-320
• /
• 1996

The role of glyoxylate bypass in a lysine-producing Corynebacterium lactofermentum strain was analyzed. Unlike the wild type, the strain expressed enzymes of glyoxylate bypass during growth in the fermentation broth containing glucose as the carbon source. To evaluate the importance of glyoxylate bypass in the strain, we disrupted chromosomal aceA by using a cloned fragment of the gene. Site-specific disruption of aceA which codes for the isocitrate lyase, the first enzyme of the bypass, was confirmed by Southern blot analysis. The aceA mutant strain completely lost isocitrate lyase activity and ability to grow in a minimal medium containing acetate as the sole carbon source. The mutant strain was similar to its parental strain in growth characteristics and produced comparable amounts of lysine in shake flasks containing glucose as the carbon source. The amount of oxaloacetate accumulated in the fermentation medium was similar for both strains, suggesting that expression of glyoxylate bypass does not necessarily lead to the increase in intracellular oxaloacetate. These data clearly demonstrate that glyoxylate bypass does not function as one of the routes of carbon supply for lysine production in the strain. It appears that the leakiness of the glyoxylate bypass in the strain might be the result of a secondary mutation which arose during previous strain development by random mutagenesis.

• 한국소성가공학회:학술대회논문집
• /
• 한국소성가공학회 1997년도 춘계학술대회논문집
• /
• pp.81-84
• /
• 1997

The hot deformation behavior of SiCp/AA2024 composites reinforced with different sizes of SiCp reinforcements (1, 8, 15, 36, and 44${\mu}{\textrm}{m}$) was investigated by hot torsion tests. The hot restoration of the composites depending on the SiCp reinforcements particle size was studied from the effective stress - strain curves. Dynamic recrystallization (DRX) was occurred in the SiCp/AA2024 composites during the hot deformation at 320 - 43$0^{\circ}C$ under a strain rate of 1.0/sec. Also, the critical strain for DRX decreased with decreasing the reinforcement size of SiCp from 44 to 8${\mu}{\textrm}{m}$. The composite reinforced with SiCp of 8${\mu}{\textrm}{m}$ showed the highest flow stress (265 MPa) and the work hardening rate at 32$0^{\circ}C$ under a strain rate of 1.0/sec.

• 센서학회지
• /
• 제22권5호
• /
• pp.315-320
• /
• 2013

In this paper, the properties of strain sensors made of spin-capable multi-walled carbon nanotubes (MWCNTs) were characterized and their sensing mechanisms analyzed. The key contribution of this paper is a new fabrication technique that introduces a simpler transfer method compared to spin-coating or dispersion CNT. Resistance of the MWCNT sheet strain sensor increased linearly with higher strain. To investigate the effect of CNT concentration on sensitivity, two strain sensors with different layer numbers of MWCNT sheets (one and three layers) were fabricated. According to the results, the sensor with a three-layer sheet showed higher sensitivity than that with one layer. In addition, experiments were conducted to examine the effects of environmental factors, temperature, and gas on sensor sensitivity. An increase in temperature resulted in a reduction in sensor sensitivity. It was also observed that ambient gas influenced the properties of the MWCNT sheet due to charge transfer. Experimental results showed that there was a linear change in resistance in response to strain, and the resistance of the sensor fully recovered to its unstressed state and exhibited stable electromechanical properties.

• 한국자동차공학회논문집
• /
• 제12권3호
• /
• pp.139-144
• /
• 2004

Dynamic crack initiation in ductile steel is investigated by means of impact loaded 3 point bend(PB) specimens. Results from non-viscoplastic and viscoplastic materials are compared. Their materials are applied with various impact velocities and static strain rates. The specimen has the size 320${\times}$750 mm with a thickness of 10 mm. A modified 3PB specimen design with reduced width at the ends has been developed in order to avoid the initial compressive load of the crack tip and also to avoid the uncertain boundary conditions at the impact heads. Numerical simulations are made by using the FEM code ABAQUS. Therefore, their results are plotted by shapes of the von Mises plastic stress and equivalent plastic strain of the specimens applied by various impact velocities.

• 한국미생물·생명공학회지
• /
• 제39권3호
• /
• pp.218-223
• /
• 2011

Eicosapentaenoic acid 생산 세균을 얻기 위해 1999~2000년 하계연구 기간 중에 남극 생물 및 침적토를 사용하여 600주의 균주를 분리하였고 TLC와 GC를 사용하여 오메가-3 고도불포화 지방산 EPA를 생산하는 미생물 7 주를 성공적으로 분리하였으며, 이중 EPA 생산이 가장 높은 L93 균주를 선발하였다. 16S rDNA의 염기서열 분석을 통하여 Shewanella 속으로 조사되었으며, 이에 분리된 균주를 Shewanella sp. L93라 명명하였다. EPA를 생산 최적 배양온도 $4^{\circ}C$이며, 초기 pH 7에서 최적 EPA 함량을 보였다. 아울러 염 농도는 50 %(w/v)에서 생산이 최대였다. EPA 최적 생산 조건을 이용하여 리터당 320 mg 생산할 수 있는 생산 시스템을 확립하였다. Urea 침전법과 HPLC을 이용하여 수율 72% 이상의 97% 순도를 가진 EPA를 정제할 수 있는 분리 정제 시스템 또한 본 연구를 통하여 확립하였다.

• 미생물학회지
• /
• 제24권1호
• /
• pp.73-78
• /
• 1986

N. gonorrhoeae 81균주를 320개의 가검물에서 분리동정한 후에 증식에 대한 VCN의 영향과 lsovitalex의 양에 따른 영향, 당분해능에 관한 실험과 ${\beta}-lactamase$생산균의 검출 및 plasmid분리에 대하여 연구하였다. 전체 320개의 가검물중에서 81균주의 N.gonorrhoeae가 분리동정되었고, chocolate agar에서는 81균주 중 72균주가,1% Isovitalex와 1 % VCN을 함유한 Thayer- Martin배지에서는 80균주가, 2 % Isovitalex와 1 % VCN이 든 Thayer-Martin 배지는 55균주가 N. gonorrhoeae의 특성을 나타냈다. Coagglutination시험에 양성인 81균주 중에서 67균주는 48시간 배양후에,10균주는 72시간 배양후에 glucose 배지에서 산을 생산하였으나, 나머지 4균주는 96시간 배양후에도 산을 생산하지 않았다. 인천지역 특수직업여성에서 분리된 81균주의 N.gonorrhoeae 중에서 41 균주가 ${\beta}-lactamase$를 생산하였고, ${\beta}-lactamase$ 생산 균주인 N. gonorrhoeae PL-118은 2.6. 4.5 및 24.5Mdal의 plasmid DNA를 포함하였다.