• Journal of Navigation and Port Research
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• v.41 no.3
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• pp.119-126
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• 2017

As container vessels get larger, container terminals are also likely to grow. The problem that arises is that the growing volume should be handled in the same amount of time as before. Container terminals are introducing an automation system in order to overcome the limitations of existing manual methods and to continuously reduce operating expenses. Because, Manual handling of carrying containers gate in and out of terminals causes inaccurate data, which results in confusion. An alternative is for containers to be labeled with barcodes that can be scanned into a system with a scanner, but this takes quite a long time and is inconvenient. A RFID system, also known as a gate automation system, can solve these problems by reducing the time of gate management with a technology that detects number identification plates, helping operators more efficiently perform gate management work. Having said that, with this system, when container damage is detected, gate operators make and keep documents manually. These documents, which are insufficient evidence in proving container damage, result in customer claims. In addition, it is difficult for gate operators and other workers to manage containers, exposing them to danger and accidents. This study suggests that if an automation system is introduced at gates, containers can be managed by a video storage system in order to better document damage The video system maintains information on container damage, allowing operators the ability to search for videos they need upon customer request, also allowing them to be better prepared for customer claims. In addition, this system reduces necessary personnel and risk of accidents near gates by integrating a wide range of work.

• Toxicological Research
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• v.28 no.1
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• pp.39-49
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• 2012

We investigated the effect of zinc on the formation of colonic aberrant crypt foci induced by azoxymethane (AOM) followed by dextran sodium sulfate (DSS) in mice with high iron diet (HFe; 450 ppm iron). Sixweek old ICR mice were fed on high iron diets with combination of three different levels of zinc in diets, low-zinc (LZn; 0.01 ppm), medium-zinc (MZn; 0.1 ppm), and high-zinc (HZn; 1 ppm) for 12 weeks. Animals were received weekly intraperitoneal injections of AOM (10 mg/kg B.W. in saline) for 3 weeks followed by 2% DSS (molecular weight 36,000~50,000) in the drinking water for a week. To confirm the iron storage in the body, the hepatic iron concentration has been determine chemically and compared with histological assessment visualized by Prussian blue reaction. Aberrant crypt (AC) and aberrant crypt foci (ACF) were analyzed in the colonic mucosa of mouse fed high dietary iron. Superoxide dismutase (SOD) activity and thiobarbituric acid-reactive substances (TBARS) level were also investigated. Apoptosis in the preneoplastic lesion was determined by terminal deoxynucleotidyl transferase-mediated dUTP nickend labeling (TUNEL). In addition, immunohistochemistry of ${\beta}$-catenin was also performed on the mucous membrane of colon. The number of large ACF (${\geq}4$ AC/ACF), which possess greater tumorigenic potential, was significantly lower in MZn and HZn groups compared with LZn group. Cytosolic SOD activity in the liver was significantly higher in HZn group compared with LZn group. Hepatic MDA level was decreased significantly in HZn group compared with MZn and LZn groups. Apoptotic index was significantly higher in HZn group. Taken together, these findings indicate that dietary zinc might exert a protective effect against colonic preneoplastic lesion induced by AOM/DSS in ICR mice with high iron status, and suggest that dietary supplement of zinc might play a role in suppressing colon carcinogenesis in mice.

• Journal of Korea Port Economic Association
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• v.36 no.1
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• pp.41-58
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• 2020

This study aims to investigate the technical efficiency of major container ports in the Bay of Bengal area and to study how certain factors influence the efficiency of container ports and terminals. The research is conducted on the four main container ports in the Bay of Bengal area, namely, Colombo Port in Sri Lanka, Chennai Port in India, Chittagong Port in Bangladesh, and Yangon Port in Myanmar. There are three input variables (quay length, storage area, and the number of cranes) and two output variables (throughput twenty-foot equivalent units and vessel calls) chosen for the process in this study. This paper evaluates the efficiency score of the defined variables and suggests implications for further improvement of the core competitiveness of the four selected ports. The findings indicate that Colombo Port is the most efficient on a technical scale, followed by Chennai Port, Yangon Port, and Chittagong Port. However, the slack and radial movement calculation results show that the inputs and outputs of the four ports need to be adjusted to be efficient and to reduce the amount of resources that are wasted. The results validate the adaptability of the improved data envelopment analysis algorithm in port efficiency analysis. The research findings provide an overview of the efficiencies of the selected container ports and can potentially affect the port management decisions made by policymakers, terminal operators, and carriers.

• Journal of Advanced Marine Engineering and Technology
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• v.40 no.7
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• pp.635-641
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• 2016

In order to drive a hybrid propulsion device which combines an engine and an electric propulsion unit, battery packs that contain dozens of unit cells consisting of a lithium-based battery are used to maintain the power source. Therefore, it is necessary to more strictly manage a number of battery cells at any given time. In order to manage battery cells, generally voltage, current, and temperature data under load condition are monitored from a personal computer. Other important elements required to analyze the condition of the battery are the internal resistances that are used to judge its state-of-health (SOH) and the open-circuit voltage (OCV) that is used to check the battery charging state. However, in principle, the internal resistances cannot be measured during operation because the parallel equivalent circuit is composed of internal loss resistances and capacitance. In most energy storage systems, battery management system (BMS) operations are carried out by using data such as voltage, current, and temperature. However, during operation, in the case of unexpected battery cell failure, the output voltage of the power supply can be changed and propulsion of the hybrid vehicle and vessel can be difficult. This paper covers the implementation of a high safety battery management system (HSBMS) that can estimate the OCV while the device is being driven. If a battery cell fails unexpectedly, a DC power supply with lithium iron phosphate can keep providing the load with a constant output voltage using the remainder of the batteries, and it is also possible to estimate the internal resistance.

• Korean Journal of Environmental Agriculture
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• v.22 no.2
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• pp.130-136
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• 2003

An organophosphorus insecticide, chlorpyrifos and an arylnitrile fungicide, chlorothalonil commonly used far apple were subjected to a residual investigation under field conditions to ensure safety of terminal residues at harvest. After pesticides were applied at standard rate in apple tree fer 15 days for chlorpyrifos and 30 days far chlorothalonil, persistence of their residues in apple was investigated by several interval. At harvest, residual concentrations of chlorpyrifos and chlorothalonil in apple were 1.3 and 2.4mg/kg, respectively, and the residue levels were higher than MRL 1.0 mg/kg in Korea. As well fitted by the first-oder kinetics, biological half-lives of the pesticide residues in apple were 9.3 days for chlorpyrifos and 32.2 days for chlorothalonil. During the storage, half-lives of chlorpyrifos and chlorothalonil were 35.0 and 56.3days at room temperature, and 120.7 and 182.8 days at 412, respectively. Distribution of chlorpyrifos residue in flesh, fruit skin and stalk cavity of each apple corresponded to 0.1% 22.8% and 77.1%, respectively. In case of chlorothalonil, residue in flesh, fruit skin and stalk cavity was 4.4%, 10.4% and 85.2%, respectively.

• Animal cells and systems
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• v.4 no.2
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• pp.157-163
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• 2000

Yeast pheromone a-factor is a 13-amino acid peptide hormone that is synthesized as a part of a larger precursor, prepro-$\alpha$-factor, consisting of a signal peptide and a proregion of 64 amino acids. The carboxy-terminal half of the precursor contains four tandem copies of mature $\alpha$-factor. To investigate the molecular basis of intracellular sorting, proteolytic processing, and storage of the peptide hormone, yeast prepro-$\alpha$-factor precursors were heterologously expressed in rat pituitary $GH_3 cells. When cells harboring the precursor were metabolically labeled, a species of approximately 27 kD appeared inside the cells. Digestion with peptide: N-glycosidase F (PNG-F) shifted the molecular mass to a 19 kD, suggesting that the 27 kD protein was the glycosylated form as in yeast cells. The nascent polypeptide is efficiently targeted to the ER in the $GH_3 cells, where it undergoes cleavage of its signal peptide and core glycosylation to generate glycosylated pro-a-factor. To look at the post ER intracellular processing, the pulse-labelled cells were chased up to 2 hrs. The nascent propeptides disappeared from the cells at a half life of 30 min and only 10-25% of the newly synthesized, unprocessed precursors were stored intracellularly after the 2 h chase. However, about 20% of the pulse-labeled pro-$\alpha$-factor precursors were secreted into the medium in the pro-hormone form. With increasing chase time, the intracellular level of propeptide decreased, but the amount of secreted propeptide could not account for the disappearance of intracellular propeptide completely. This disappearance was insensitive to lysosomotropic agents, but was inhibited at $16^{circ}C or 20^{\circ}C$, suggesting that the turnover of the precursors was not occurring in the secretory pathway to trans Golgi network (TGN) or dependent on acidic compartments. From these results, it is concluded that a pan of these heterologous precursors may be processed at its paired dibasic sites by prohormone processing enzymes located in TGN/secretpry vesicles producing small peptides, and that the residual unprocessed precursors may be secreted into the medium rather than degraded intracellularly.

• Korean Journal of Microbiology
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• v.52 no.2
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• pp.230-235
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• 2016

This study was carried out to analyze the differences in enzyme activity and stability between the native Fe superoxide dismutase (FeSOD) and the 6xHis-tagged superoxide dismutase (6xHis-FeSOD) of Streptomyces subrutilus P5. The optimum pHs for both native FeSOD and 6xHis-FeSOD were 7, while the pH range of the activity was narrower for the 6xHis-FeSOD. The native FeSOD was stable at pH 4-9, but the 6xHis-FeSOD lost its stability at pH > 9. The temperatures of the optimum activities were same for both types of enzymes. However, the heat stability of the 6xHis-FeSOD was clearly decreased; even at $20^{\circ}C$ the enzyme lost the activity after 360 min. In contrast, the native FeSOD was stable after 720 min at below $40^{\circ}C$. $H_2O_2$ inhibition was occurred already at 0.5 mM for the 6xHis-tagged enzyme. Therefore, from the results that the 6xHis-FeSOD retained the enzyme activity at pH 6-7 and $20-40^{\circ}C$, it can be assumed that the protein structure became destabilized under different storage conditions and sensitive to the enzyme inhibitor.

• Tunnel and Underground Space
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• v.31 no.1
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• pp.52-65
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• 2021

In this study, authors analyzed the vapor cloud explosion induced by propane leak at the PEMIX Terminal, which is the propane storage facility outside of Mexico City. TNT equivalence mass for the leaked 4750 kg propane was estimated to be 9398 kg. Blast parameters such as peak overpressure, positive phase duration, and impact at 40-400 (m) away from the center of the explosion were calculated by applying TNT Equivalency Method and Multi-Energy Method. The probability of damage due to lung damage, eardrum rupture, head impact, and whole-body displacement impact by applying the probit function obtained using blast parameters was evaluated. The peak overpressure obtained using Multi-Energy Method was found to be greater than the peak overpressure obtained by applying the TNT Equivalency Method at all distances considered, but it was evaluated that there was no significant difference from the points above 200 m. The peak overpressure obtained by Multi-Energy Method was computed to assess the extent of damage to the structure, and it was shown that structures within 100 m of the explosion center would collapse completely, and that the glasses of the structures 400 m away would be almost broken. The probability of death due to lung damage was shown to vary depending on a human body's position located in the propagating direction of shock wave, and if there is a reflecting surface in the immediate surroundings of a human body, the probability of death was estimated to be the greatest. The impact of shock wave on lung damage, eardrum rupture, head impact, and whole-body displacement impact was evaluated and found to affect whole-body impact < lung damage < eardrum rupture