• The Korean Journal of Ecology
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• v.4 no.3_4
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• pp.59-67
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• 1981

Stomatal resistances of the leaves in Heteromeres arbutifolia and of the stems in Ferocactus acanthodes were studied to estimate active and passive behaviors of the guard cells on a theoretical basis. Active and passive stomatal responses to light and water deficit were observed. When the change rate of existent water due to variation of osmotic potential in the guard cells and the loss rate of transpirational water from the guard cells are $\Delta$wi-$\Delta$wt and leaded to active behaviors for opening and closing stomata. However, when stems of F. acanthodes with stomata closecd under the solar irradiation were covered with black cloth and then taken off, behaviors of the guard cells occurred in the condition of $\Delta$wi<$\Delta$wt and were passive. Under the conditiion of $\Delta$wi<$\Delta$wt due to cutout from stems, passive behaviors of the guard cells in H. arbutifolia and F. acanthodes always occurred in spite of the solar irradiation and darkness, respectively. The transpirational resistance coefficients of the guard cells in stems of F. acanthodes (0.380) and Opuntia bigelovii (0.135) wer emuch higher than in leaves of H. arbutifolia (0.034). Moreover, stomatal opening in stems of F. acanthodes during the daytime could be induced by watering. Those results are interpreted as that since the guard cells in desert Crassulacean acid metabolism (CAM) plants always exist in the state of stomatal opening, nocturnal stomatal opening and daytime stomatal closing are exhibited by passive behaviors of the guard cells in the alternant conditioins of $\Delta$wi>$\Delta$wt and $\Delta$wi<$\Delta$wt, respectively.

• Korean Journal of Medicinal Crop Science
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• v.26 no.2
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• pp.181-187
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• 2018

Background: This study aimed to investigate out the influence of drought stress on the physiological responses of Dendropanax morbifera seedlings. Methods and Results: Drought stress was induced by discontinuing water supply for 30 days. Under drought stress, photosynthetic activity was significantly reduced with decreasing soil water content (SWC), as revealed by the parameters such as Fv/Fm, maximum photosynthetic rate ($P_{N\;max}$), stomatal conductance ($g_s$), stomatal transpiration rate (E), and intercellular $CO_2$ concentration (Ci). However, water use efficiency (WUE) was increased by 2.5 times because of the decrease in $g_s$ to reduce transpiration. Particularly, E and $g_s$ were remarkably decreased when water was withheld for 21 days at 6.2% of SWC. Dendropanax morbifera leaves showed osmotic adjustment of -0.30 MPa at full turgor and -0.13 MPa at zero turgor. In contrast, the maximum bulk modulus of elasticity ($E_{max}$) did not change significantly. Thus, Dendropanax morbifera seedlings could tolerate drought stress via osmotic adjustment. Conclusions: Drought avoidance mechanisms of D. morbifera involve reduction in water loss from plants, through the control of stomatal transpiration, and reduction in cellular osmotic potential. Notably photosynthetic activity was remarkably reduced, to approximately 6% of the SWC.

• Journal of Environmental Science International
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• v.23 no.5
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• pp.763-770
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• 2014

The effect of $CO_2$ on the opening of stomata in the intact leaf of Commelina communis has been investigated. Full opening of stomatal apertures(around $18{\mu}m$) was achieved in the intact leaf by addition of $CO_2$($900{\mu}mol\;mol^{-1}$). At 90 minutes, the stomatal apertures of leaves treated with $CO_2$ free air were reduced. In contrast, stomata opened most widely with the treatment of $CO_2$ air at 90 minutes. The effects of light, $CO_2$ air and $CO_2$ free air on the change of membrane potential difference(PD) were measured. Fast hyperpolarization of guard cell membrane PD was recorded reaching up to -12 mV in response to light. If $CO_2$ free air was given firstly, there was no response. When light was given after $CO_2$ free air, the light effect was very clear. At the onset of $CO_2$ air, the PD showed a dramatic hyperpolarization to about -25 mV. Changes in the pH of apoplast in intact leaves in response to $CO_2$ air were observed. $CO_2$ air caused a change of 0.4 pH unit. Therefore, it can be hypothesized that $CO_2$ flowing could stimulate proton efflux which is a necessary precursor of stomatal opening.

• Journal of Bio-Environment Control
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• v.15 no.1
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• pp.100-106
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• 2006

A hypertonic solution of sorbitol was used to precondition Douglas-fir and Western hemlock plug seedlings to improve desiccation resistance. Seedlings were preconditioned by soaking their root balls in water, -0.75 or -1.50 MPa sorbitol solution for 22 hr, and then exposed to desiccation conditions for 8 days. During the desiccation period, a transpirational water loss was significantly reduced by the sorbitol preconditioning, with its effect positively depending on concentration. This preconditioning-induced reduction in water loss was mainly caused by the decline in needle stomatal conductance. Sorbitol-induced stomatal control was more closely associated with reduction in plant water potential, rather than increase in abscisic acid concentrations. After rehydration of stressed-plants, most of the preconditioned seedlings with sorbitol were survived, while only 35% of Douglas-fir and 28% of Western hemlock seedlings treated with water were alive. The post-growth was significantly greater in the preconditioned seedlings than only water-treated seedlings. These results suggested that the earlier stomatal control with sorbitol-facilitated preconditioning could play a role in improving desiccation resistance of evergreen woody plants at transplanting in the field where water supply is limited or dry conditions are prevailing.

• Korean Journal of Environmental Biology
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• v.20 no.1
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• pp.78-84
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• 2002

The effects of light and darkness on stomatal aperture and guard cell apoplastic pH in the intact leaf and in the isolated epidermal strips of Commelina communis have been investigated. Stomata in the intact leaf opened wide in the light. In contrast, stomata in the isolated epidermal strips did not respond clearly to light. To eucidate the relationship between the stomatal aperture and the guard cell apoplastic pH, apoplastic pH was measured. In the light the guard cell wall of intact leaf was acidified by pH 1.9 units, falling from pH 7.3 to pH 5.4 in the first 10 minutes. On the contrary, apoplastic pH of isolated epidermal strips changed slowly from pH 7.3 to pH 6.9 at 20 min. Stomata in the intact leaf closed rapidly in the dark. On the other hand, stomata in the isolated epidermal strips failed to close in dark. There was a slow increase in apoplastic pH on transfer to the dark after incubation for 1.5 h in the light and the level observed before the experiment was regained after around 40 min. When the isolated epidermal strips were transferred to the dark, apoplastic pH maintained a uniform level of around pH 7.2-7.4. These results indicate that the mechanism of stomatal opening and closing from isolated epidermal strips and intact leaves could be different.

• International Journal of Industrial Entomology and Biomaterials
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• v.7 no.2
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• pp.175-180
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• 2003

Changes in biochemical constituents and physiological alteration were studied in various intensities (1-5%, 6-15%, 16-30%, 31-50% and ＞ 50%) of leaf spot (Myrothecium roridum) on mulberry leaves and compared with healthy leaves. Chlorophyll, total soluble sugar and total protein were decreased (P ＜ 0.01), but total phenol increased due to pathogen infection. Changes in biochemical constituents showed significant correlation with intensity of disease. Chlorophyll ($r^2$= 0.92), and protein (($r^2$= 0.83) possessed negative while phenol (($r^2$= 0.61) possessed positive correlation. Photosynthetic rate, transpiration rate, stomatal conductance, moisture content (%) and physiological water use efficiency (pWUE) were decreased, but stomatal resistance increased in the infected leaves. Physiological parameters also possessed significant (P ＜ 0.01) correlation with disease intensity. Photosynthetic rate (($r^2$= 0.96), transpiration rate ($r^2$=0.88), stomatal conductance (($r^2$= = 0.65), physiological water use efficiency (($r^2$= 0.88) and moisture content (r = 0.85) were negatively but stomatal resistance (($r^2$= 0.75) was positively correlated to disease intensities.

• Journal of Ginseng Research
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• v.4 no.1
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• pp.49-54
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• 1980

This investigation were carrion out to know the frequency, size and distribution of stomata in Korean ginseng, acanthopanax and codonopsis. The results are as follows; 1. Stomatal frequency in ginseng leaf was remarkably less than those of acanthopanax and codonopsis leaf, but size of stomata in ginseng leaf was larger than those of acanthopanax and codonopsis leaf. 2. Stomatal frequency of one year old ginseng plant was higher than those of the older. Two to five years old ginseng plants were not differed in frequency and size of stomata. 3. Frequency and size of stomata were higher and larger in red-berry variant in compare to yellow-berry variant. 4. Stomatal frequency in different leaf Position was not significantly different among those of middle leaf, first side leaf and second side leaf, but in decreasing order of middle Part, upper, lower part and edge in the same ginseng leaf. 5. Stomata was not seen in adaxial surface and petiole of leaf ginseng, acanthopanax and codonopsis. 6. Stomatal frequency was higher in ginseng plant grown under no$.$shading compared to shading, and that of ginseng plant in rear line was less than that of front line under the same shade roof.

• The Korean Journal of Ecology
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• v.28 no.2
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• pp.105-112
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• 2005

Environmental factors such as light and low $CO_2$ concentrations trigger events which may result in stomatal opening. Stomatal aperature is largely controlled by the solute contents of guard cells, but not exclusively, by through changes in their content of potassium salts, with $K^+$ balanced either by $Cl^-$ or malate, depending on the species and conditions. However, how these signals are sensed and how they are transduced into driving the ion fluxes that control stomatal movements is not still fully understood. The basic role of stomata is regulating transpiration and photosynthesis. Photosynthesis plays a central role in the physiology of plants and an understanding of its response to light is, therefore, critical to any discussion of how plants sense and respond to light. It had been proposed that the evidences pointed three possible mechanisms for the light response. Firstly, there is a direct response of stomata to light. Secondly. there is an indirect response of stomata to light through the effect of $CO_2$. Lastly, there are some evidences for a third effect of light on stomata. However, attempts to investigate how these three possible mechanisms explained in detail in response to light have not been made. Therefore, this study is examined the differences among these three possible mechanisms.

• The Korean Journal of Ecology
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• v.17 no.3
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• pp.241-249
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• 1994

Leaf diffusive resistance (LDR), stomatal density, length of guard cell and hair density of leaves of 6 oak species were determined under withdrawal of water, and their strategies of drought stress were analyzed by principal component analysis. LDR of Quercus acutissima, Q. aliena and Q. serrata increased earlier than those of the other species at high leaf water potential $({\Psi}_{leaf})$ or low water saturation deficit (WSD), which was an avoidance mechanism reducing damage by water stress. Q. variabilis with low stomatal density, small stomatal size and high hair density had avoidance mechanisms increasing LDR at high $({\Psi}_{leaf})$ However, Q. mongolica and Q. dentata increased LDR at low $({\Psi}_{leaf})$ as xeric species do. Results from principal component analysis on the 15 variables related to strategies of drought stress indicated that the 6 oak species were divided into 2 groups: (1) Q. acutissima, Q. aliena and Q. serrata as mesic habitat species and (2) Q. variabilis, Q. mongolica and Q. dentata as xeric habitat species. Among three xeric species Q. acutissima differed from the other two species in the drought strategies such as high hair density, low stornatal density, high leaf area ratio, stomatal closing at low $({\Psi}_{leaf})$ and small cell wall elasticity. The results could reasonably explain their drought strategies in natural habitat.

• Korean Journal of Agricultural and Forest Meteorology
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• v.1 no.1
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• pp.52-59
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• 1999

Vegetation canopy plays an important role in $CO_2$/$H_2$O exchange between the biosphere and the atmosphere by controlling leaf stomata. In this study, rice (Oryza sativa L.), a staple crop in Asia was investigated to formulate its single leaf model of photosynthesis and stomatal conductance. Photosynthesis and stomatal conductance were measured with a portable infrared gas analyzer system. Other plant and meteorological variables were also measured. To evaluate empirical constants in this biochemical leaf model, nonlinear least squares technique was used. The maximum catalytic activity of enzyme and the maximum rate of electron transport were $ 100\mu$㏖ $m^{-2}$ $s^{-1}$ and $140 \mu$㏖ m$^{-2}$ s$^{-1}$ (@ 35$^{\circ}C$), respectively. The empirical constants, m and b, associated with stomatal conductance model were 9.7 and $0.06 m^{-2}$ $s^{-1}$ , respectively. On a leaf scale, agreements between the modeled and the measured values of photosynthesis and stomatal conductance were on average within 20%, and the simulation of diurnal variation was also satisfactory On a canopy scale, the Simple Biosphere model(SiB2) was tested using the derived parameters. The modeled energy fluxes were compared against the micrometeorologically measured fluxes over a rice canopy. Agreements between the modeled and the measured values of net radiation, sensible heat and latent heat fluxes, and $CO_2$ flux (i.e., net canopy photosynthesis) were on average within 25%.