• Applied Biological Chemistry
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• 제46권4호
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• pp.376-379
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• 2003

쑥갓의 지상부를 MeOH로 추출하고, 추출물을 EtOAc, n-BuOH 및 물로 분배, 추출하였다. EtOAc 분획으로부터 silica gel column chromatography를 반복하여 3종의 sterol을 분리하였다. 각각의 화학구조는, NMR및 MS등의 스펙트럼을 해석하여, $stigmast-5-en-3{\beta}-ol\;(1,\;{\beta}-sitosterol),\;stigmast-4-en-6{\beta}-ol-3-one$ (2), $stigmast-4-en-6{\alpha}-3-one$ (3)으로 동정하였다. 화합물 2와 3은 육상식물에서는 처음으로 분리되었다.

• 생약학회지
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• 제31권1호
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• pp.109-111
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• 2000

Two compounds were isolated from the root of Astragalus membranaceus(Bunge). On the basis of spectroscopic data their structures were identified as stigmast-4-en-3-one and a mixture of 2'-angeloyloxy-1',2'-dihydroxanthyletin and 2'-senecioyloxy-1',2'-dihydroxanthyletin. They were first isolated from the genus of Astragalus.

• Journal of the Korean Wood Science and Technology
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• 제30권4호
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• pp.51-57
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• 2002

항산화활성이 우수한 아까시나무 심재 에탄올 조추출물로부터 항산화물질을 탐색할 목적으로 4종의 화합물을 단리하였으며, MS, NMR 등의 분광학적 방법에 의해 그 화학적 구조를 3-𝛽-stigmast-5-en-3-ol(daucosterol)를 비롯하여 스틸베노이드 화합물인 3,3',4',5-tetrahydroxystilbene과 후라보노이드인 3,3',4',5',7-pentahydroxyflavone(robinetin) 및 3,3',4',7-tetrahydroxyflavanone(fustin)으로 각각 동정하였다. 단리된 화합물의 프리라디칼 소거능을 측정하여 항산화활성을 검정한 결과, 페놀성 수산기가 5개인 3,3',4',5',7-pentahydroxyflavone은 천연항산화제 및 합성항산화제보다 높은 항산화활성을 나타내어 단리화합물 중 가장 항산화활성이 높은 것으로 나타났다. 또한, 페놀성 수산기가 4개인 3,3',4',5-tetrahydroxystilbene과 3,3',4',7-tetrahtdroxyflavanone도 천연항산화제인 𝛼-tocopherol보다 높은 항산화활성을 나타내었으나 3-𝛽-stigmast-5-en-3-ol은 항산화활성이 없는 것으로 나타나 프리라디칼 소거능에 의한 항산화활성은 화합물의 페놀성 수산기 수에 크게 좌우되는 것으로 판단되었다. 이상의 결과 아까시나무의 높은 항산화활성은 3,3',4',5',7-pentahydroxyflavone, 3,3',4',5-tetrahydroxystilbene 및 3,3',4',7-tetrahydroxyflavanone에 기인하는 것으로 사료되었다.

• Archives of Pharmacal Research
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• 제28권10호
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• pp.1147-1151
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• 2005

A new naturally occurring sterol, compound 5, and six known stigmasterols were isolated from fruits of Ailanthus altissima Swingle by repeated column chromatography and RP-HPLC. Their structures were identified as, 5${\alpha}$-stigmastane-3,6-dione (1), 3${\beta}$-hydroxystigmast-5-en-7-one (2), stigmast-5-ene-3${\beta}$, 7${\alpha}$-diol (3), 6${\alpha}$-hydroxystigmast-4-en-3-one (4), 5${\alpha}$-stigmastane-3${\beta}$, 6${\beta}$-diol (5), stigmast-4-ene-3${\beta}$, 6${\alpha}$-diol (6), stigmast-5-ene-3${\beta}$, 7${\alpha}$, 20$\xi$-triol (7) by spectral analysis and comparison with the published data. These compounds have not been reported from genus Ailanthus, whereas compound 7 was identified by NMR for the first time. In addition, the $95\%$ ethanol extract and compounds from the fruits of Ailanthus altissima SWINGLE were assayed for in vitro antimicrobial activity. The extract was potent active against the assayed bacteria while compounds 3 and 7 exhibited moderate activity.

• Archives of Pharmacal Research
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• 제28권3호
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• pp.294-296
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• 2005

Repeated chromatography of the n-hexane-soluble fraction of the MeOH extract of the rhizomes of Zingiber zerumbet led to the isolation of two isomers of 6-methoxy-2E,9E-humula-dien-8-one (1 and 2) and stigmast-4-en-3-one. The structures of 1 and 2 were determined by spectroscopic methods including 10 and 2D-NMR elucidated by analysis of spectroscopic data as well as by comparison with published values. This is the first report on the isolation of compounds 1 and 2 from the nature. Stigmast-4-en-3-one was first isolated from this plant.

• 생약학회지
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• 제27권4호
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• pp.336-341
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• 1996

The roots of Korean Astragalus membranaceus Bunge. (Leguminosae), Astragali Radix, were extracted following general methods. During these extractions, first of all, 1,2-benzendicarboxylic acid diisononylester(compound A), ${\beta}-sitosterol(compound\;B)$, $3-0-{\beta}-D-galactopyranosyl-{\beta}-sitosterol(compound\;C)$ and $stigmast-4-en-6{\beta}-ol-3-one(compound\;D)$ were isolated. Three of these compounds were identified the first time isolation from the genus of Astragalus.

• Applied Biological Chemistry
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• 제49권1호
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• pp.82-85
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• 2006

산딸나무 열매를 100% MeOH로 추출하고, 얻어진 추출물을 EtOAc, n-BuOH 및 물로 용매 분획 하였다. 이 중 EtOAc 분획으로부터 silica gel과 octadecyl silica gel(ODS) column chromatography를 반복하여 3종의sterol 화합물을 분리, 정제하였다. 각 화합물의 화학구조는 NMR, MS 및 IR 등의 스펙트럼 데이터를 해석하여, ${\beta}-sitosterol$(1), $stigmast-4-en-6{\beta}-ol-3-one$(2), daucosterol(3)으로 동정하였다. 이 화합물들은 산딸나무에서 처음 분리되었다.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.204.3-205
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• 2003

Antimutagenic activity-guided fractionation of an extract prepared from the thorns of Gleditsia sinensis Lam. (Leguminosae), which is a perennial shrub widely distributed throughout China (called "Zao Jiao Ci" ) and in Gyeongju city area in Korea (called "Jo Gak Ja" ) and has been used in traditional medicine for the treatment of swelling, suppuration, carbuncle and skin diseases, led to the isolation of one triterpenoid and four steroids, which were identified as D:C-friedours-7-en-3-one (1), stigmastane-3,6-dione (2), ${\beta}$-sitosterol (3), sigmasterol (4), and stigmast-4-en-3,6-dione (5). (omitted)

• 생약학회지
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• 제27권1호
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• pp.75-79
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• 1996

Four steroids and one flavonol glycoside were isolated from the ethanol extract of the whole plant of Aconitum pseudolaeve var. erectum. Their structures were identified as ${\beta}-sitost-4-en-3-one$, 22-dihydro-stigmast-4-en-3,6-dione, ${\beta}-sitosterol$, ${\beta}-sitosterol-3-O-{\beta}-D-glucopyranoside$ and $kaempferol-3-O-{\beta}-D-glucopyranoside(astragalin)$ on the basis of spectral data.

• Natural Product Sciences
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• 제13권4호
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• pp.332-336
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• 2007

Five steroids and two norsesquiterpene glycosides were isolated from the methanol extract of H. japonicus. Their structures were determined by means of physio-chemical and spectral data to be friedelin (1), stigmast-5-en-3-${\beta}$-ol (${\beta}$-sitosterol) (2), 7-keto-${\beta}$-sitosterol (3), 6${\beta}$-hydroxy-4-stigmasten-3-one (4), 7${\alpha}$-hydroxy-${\beta}$-sitosterol (5), 3-hydroxy-4,4-dimethyl-4-butyrolactone (6), daucosterol (7), (6S, 9S)-roseoside (8), and (9S)-drummondol-9-O-${\beta}$-D-glucopyranoside (spinoside B) (9). The compounds 1, 3, 4, and 6 - 9 were first isolated from this plant source.