• Title/Summary/Keyword: sticky

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Development of Genetic System for Isolation of SSU rRNA Mutants that Bypass SecM-Mediated Ribosome Stalling (SecM에서 유래한 접착펩타이드에 의한 라이보솜 정지를 우회하는 SSU rRNA 돌연변이체 발굴을 위한 유전학적 시스템 개발)

  • Ha, Hye-Jeong;Kim, Hong-Man;Yeom, Ji-Hyun;Lee, Kang-Seok
    • Korean Journal of Microbiology
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    • v.44 no.4
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    • pp.271-276
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    • 2008
  • Ribosome stalling by nascent sticky peptide has been reported in several organisms across the kingdom. To test whether small subunit (SSU) rRNA is involved in this phenomenon, we developed a genetic system that utilized the specialized ribosome system to isolate SSU rRNA mutants that enable ribosomes to bypass the SecM-derived sticky peptide in protein synthesis. In this system, CAT-SecM mRNA, which encodes CAT protein containing the sticky peptide derived from SecM, is only translated by specialized ribosomes. These ribosomes were shown to transiently stall on CAT-SecM mRNA followed by the synthesis of the sticky peptide. Expression of specialized ribosomes resulted in the decreased steady-state level of CAT-SecM mRNA, which is consistent with a notion that ribosome stalling induces mRNA degradation. Isolation and characterization of SSU rRNA mutations using this genetic system that are sufficient to circumvent ribosome stalling induced by the SecM-derived sticky peptide will provide evidence of SSU rRNA function in mRNA cleavage.

Limitation in Attraction Efficacy of Aggregation Pheromone or Plant Volatile Lures to Attract the Western Flower Thrips, Frankliniella occidentalis Infesting the Hot Pepper, Capsicum annuum, in Greenhouses (시설 고추재배지에서 꽃노랑총채벌레 집합페로몬과 식물 휘발성 유인제 효능의 한계성)

  • Kim, Chulyoung;Gwon, Gimyeon;Kim, Yonggyun
    • Korean journal of applied entomology
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    • v.60 no.4
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    • pp.369-377
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    • 2021
  • Mass trapping of the western flower thrips, Frankliniella occidentalis, has been considered as an option to control this pest. This study applied the commercial lures to the hot pepper-cultivating greenhouses and assessed the enhancement of the attracting efficiency by adding to sticky traps. There was no color difference in the attracting efficiency between blue and yellow sticky traps. However, the installation position of the traps was crucial in the greenhouses. The more thrips were captured within host cropping area than outside areas of the crop. In vertical trap position, it was the most optimal to install the traps at the crop crown. Using these installation parameters, the yellow sticky traps captured approximately 1% population of the thrips. To enhance the trapping efficiency, the commercial lures containing aggregation pheromone or 4-methoxybenzaldehyde were added to the yellow sticky traps. However, these commercial lures did not significantly enhance the trapping efficiency compared to the yellow sticky trap alone. In contrast, Y-tube olfactometry assays confirmed the high efficiency of the aggregation pheromone or another plant volatile (methyl isonicotinate) to attract the thrips. Interestingly, these lure components had lower attracting efficiencies compared to the hot pepper flowers. The high attractive efficiency of the flowers was supported by the observation that the commercial lure was effective to enhance the trapping efficiency of the yellow sticky trap against F. occidentalis in Welsh onion (Allium fistulosum) field without any flowers. This study indicates the limitation of the commercial lures in application to hot pepper fields for the mass trapping of F. occidentalis. It also suggests active volatile component(s) from hot pepper flowers to attract F. occidentalis.

Free molecule transmission probability of a conical tube with wall sorption

  • 인상렬
    • Journal of Korean Vacuum Science & Technology
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    • v.2 no.1
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    • pp.1-8
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    • 1998
  • The uniform distributed pumping model is used to derive analytic expressions of the pressure profile for the molecular flow regime in linearly tapered or flared(conical or pyramidal) tubes with wall sorption. The concept of transmission conductance for sticky tubes of arbitrary shape is newly introduced to calculate the transmission probability using the pressure profile. The transmission probability obtained analytically for a conical sticky tube is compared with that from the Monte Carlo simulation.

Implementation and Verification of Linear Cohesive Viscoelastic Contact Model for Discrete Element Method (선형 부착성 점탄성 접촉모형의 DEM 적용 및 해석적 방법을 이용한 검증)

  • Yun, Tae Young;Yoo, Pyeong Jun
    • International Journal of Highway Engineering
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    • v.17 no.4
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    • pp.25-31
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    • 2015
  • PURPOSES: Implementation and verification of the simple linear cohesive viscoelastic contact model that can be used to simulate dynamic behavior of sticky aggregates. METHODS: The differential equations were derived and the initial conditions were determined to simulate a free falling ball with a sticky surface from a ground. To describe this behavior, a combination of linear contact model and a cohesive contact model was used. The general solution for the differential equation was used to verify the implemented linear cohesive viscoelastic API model in the DEM. Sensitivity analysis was also performed using the derived analytical solutions for several combinations of damping coefficients and cohesive coefficients. RESULTS : The numerical solution obtained using the DEM showed good agreement with the analytical solution for two extreme conditions. It was observed that the linear cohesive model can be successfully implemented with a linear spring in the DEM API for dynamic analysis of the aggregates. CONCLUSIONS: It can be concluded that the derived closed form solutions are applicable for the analysis of the rebounding behavior of sticky particles, and for verification of the implemented API model in the DEM. The assumption of underdamped condition for the viscous behavior of the particles seems to be reasonable. Several factors have to be additionally identified in order to develop an enhanced contact model for an asphalt mixture.

Removal Performance of Sticky Paint Aerosol Control System Generated from Small Scale Car Paint Overspray Booth (소형 자동차 페인트 도장부스에서 발생하는 점착성 paint aerosol 처리장치에서 제거성능)

  • Lee, Jae-Rang;Hasolli, Naim;Jeon, Seong-Min;Lee, Kang-San;Sohn, Jong-Ryeul;Park, Young-Ok
    • Journal of Korean Society for Atmospheric Environment
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    • v.31 no.1
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    • pp.54-62
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    • 2015
  • Small scale paint overspray booths are being operated nationwidely, for repair of passenger car body parts. paint aerosols are emitted from the paint overspray booth in operations. In paint overspray booth operations without ventilation system and air pollutants collection unit, it may land on nearby equipment. In this study a removal of sticky paint aerosol for application of the small-scale overspray paint booth. it's cause the surface of filter bag from generated sticky paint aerosol. To remove adhesion of paint aerosol the agglomerating agents are injected and mixed with sticky paint aerosols prior to reach the filter bag. The paint spray rate was set as $10{\pm}5g/min$ from air-atomized spray guns in the spray booth, injection rate of agglomerating was $10{\pm}5g/min$ in the mixing chamber. The filtration velocity including air pollutants varied from 0.2 m/min to 0.4 m/min. Bag cleaning air pressure was set as $5.0kg_f/min$ for detaching dust cake from surface of filter bag. Bag cleaning interval at the filtration velocity of 0.2 m/min was around 3 times longer than that of the 0.4 m/min. The residual pressure drop maintained highest value at the highest filtration velocity. Fractional efficiency of 99.952%~99.971% was possible to maintain for the particle size of 2.5 microns. Total collection efficiency at the filtration velocity of 0.2 m/min was 99.42%. During this study we could confirm high collection efficiency and long cleaning intervals for the test with filtration velocity of 0.2 m/min indicating an optimal value for the given dimensions of the test unit and test operating conditions.

Environmentally-friendly Control Methods and Forecasting the Hatching Time Lycorma delicatula (Hemiptera: Fulgoridae) in Jeonnam Province (전남지역에서 꽃매미 부화시기 예측과 친환경 방제방법)

  • Choi, Duck-Soo;Kim, Do-Ik;Ko, Suk-Ju;Kang, Beom-Ryong;Park, Jong-Dae;Kim, Seon-Gon;Choi, Kyeong-Ju
    • Korean journal of applied entomology
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    • v.51 no.4
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    • pp.371-376
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    • 2012
  • This study was conducted to predict the hatching time of eggs of Lycorma delicatula, to select an effective environmentally-friendly agriculture material (EFAM) and to evaluate the attraction effect of brown sticky traps for controling of Lycorma delicatula nymph and adults. Eggs hatched 55.9, 26.8, 21.6 days after incubation at 15, 20, $25^{\circ}C$ with 14L:10D condition and the hatching rates of egg were 61.9, 57.8, 30.4%, respectively. At high temperature conditions, egg development periods were shorter and the hatching rate was lower. The relationship between temperature and developmental rate was expressed by the linear equation Y=0.0028X-0.0228, $R^2$=0.9561. The low temperature threshold of eggs was $8.14^{\circ}C$ and the thermal constant required to reach larva was 355.4 DD. According to this relationship, the mean estimated hatching date was $22^{nd}$ May. The effective EFAM was natural plant extract, sophora extract, derris extract to nymph and natural plant extract, pyrethrum extract, sophora extract to adult. Among three colors of sticky trap : brown, blue and yellow, the brown sticky trap was the most attractive to nymphs and adults of L. delicatula over a 2 weeks trial period. It suggested that the brown sticky trap could be a very useful and environment-friendly control method for nymphs and adults of L. delicatula.

Occurrence and Damage of Bradysia agrestis Sasakawa (Diptera: Sciaridae) in Propagation House (육묘장내 작은뿌리파리의 발생과 피해)

  • 김형환;추호렬;이홍수;조성래;신현열;박정규;추영무
    • Korean journal of applied entomology
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    • v.39 no.2
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    • pp.89-97
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    • 2000
  • Insect pests of eggplant, pepper, Chinese cabbage, watermelon, cucumber, melon, and tomato seedlings were surveyed in eleven propagation houses from 1998 to 1999. Ten species of insect pests of nine genera in eight families, two species of mites of one genus in one family, and three species of slugsor snails in three genera belonging to two families were found from the observed seedlings but Bradysia agrestis was the most serious pest out of them. Thus, occurrence and damage of B. agrestis were investigated in two propagation houses all the year round. In the observation of seedling mortalities of seven crops in May, July, October and the following February, only watermelon seedlings were withered and dead by the larva of B. agrestis with different mortality depending on time representing 81.9%, 41.3%, 54.9%, and 79.1%, respectively. All the developmental stages of B. agrestis were found every month throughout the year. Larval density and adult density were different according to season with the highest numbers in May showing 34.9 and 407.4, respectively. Mortality of watermelon seedlings was higher in April and May than the other months showing 83.3% in April and 82.4% in May. The number of adults attracted to sticky trap was also different depending on card colour. The number of adults was much higher on yellow sticky card (326.2) than blue sticky card (20.2). In the investigation of the number of adults on yellow sticky card at 25 cm and 120 cm above the bench and 10 cm under the bench, more numbers of adults were caught at 25 cm above the bench (273.7) and 10 cm under the bench (320.1) than 120 cm above the bench (27.9). Mortalities of cucumber, pepper, and watermelon seedlings after transplanting in greenhouses were not significantly different depending on culture method.

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Scanning Electronic Microscopy Examination for the Egg of Skin Parasite, Entobdella hippoglossi on the Commercially Important Culture Fish, the Atlantic Halibut, Hippoglossus hippoglossi

  • Yoon Gil Ha
    • Fisheries and Aquatic Sciences
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    • v.1 no.1
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    • pp.68-71
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    • 1998
  • The egg structure and shape of the skin parasite, Entobdella hippoglossi from the Atlantic halibut, Hippoglossus hippoglossus was examined using scanning electronic microscope. The newly produced eggs of E. hippoglossi were yellowish in color and tetrahedral in shape. The eggs were entwined together in a complicated chain-like fashion by a long filament. The appendage showed the presence of buoy-like structures. The buoy-like structures on the appendage of the eggs of E. hippoglossi in the present study were totally different from other sticky droplets. It is suggested that these structures are not sticky droplets but buoys for floating eggs which have entwined together on the bottom of the sea. It seems that these buoy-like structures may be needed for preventing the eggs from being covered by mud or particles and thus maintaining the eggs in an oxygenated environment.

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Quantification Method of Macro Stickies for Recycled Pulps (재생펄프의 점착성 이물질 정량 분석 방법)

  • 윤병태;오세균
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.31 no.2
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    • pp.86-91
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    • 1999
  • A new test method has been developed for a quantitative evaluation of macro-stickies in recycled pulps. The method uses a fine slot screen to separate a sticky particles form fiber in the pulp samples. The obtained sticky particles were spread on filter paper and covered with silicon carbide powder in order to make the stickies area with sufficient contrast between stickies and background. The stickies are then counted by a scanner-based image analysis system. The validity of this evaluation method of stickies of KOCC, ONP and white ledger wastepaper recycling plants respectively. It is shown that this method can be applied for a quantitative determination of stickies content in various recycled pulp samples. With this testing method, reproducible and reliable data were obtained for the actual mill pulp samples.

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