• Journal of Biosystems Engineering
• /
• v.28 no.5
• /
• pp.449-456
• /
• 2003

A robot system was developed to pick desired colony. This robot system consisted of an image acquisition process which acquires the image information of colony, an illumination device which irradiates the object once when it gets the image of it, a picking head, a replicating head, a bed for fixing well-plates, and a sterilization device of sterilizing pin stained with colony. Picking pins were washed in an ultrasonic wave washing for takes ten seconds. Picking pins were dipped for sterilizing in alcohol for ten seconds. The time for resterilizing picking pins in a heater was five seconds. This performance test resulted 100% success rate for both the colony picking and the colony replication process. Considering the procedure in which 8 pins were smeared by colony, picked on a well plate, and sterilized as one cycle, the system could carry out one cycle in 110 seconds. It took about 138 minutes to pick around 600 colonies.

• Asian Oncology Nursing
• /
• v.12 no.4
• /
• pp.331-338
• /
• 2012

Purpose: This descriptive study aimed to compare the perception of the acceptable foods for the neutropenic diet between nurses and patients by food type. Methods: The participants were 225 nurses working at chemotherapy wards and 71 patients in chemotherapy treatment. Data were collected with a self-administered questionnaire from January 2 to February 24, 2012, and analyzed with SPSS 12.0 program using descriptive statistics and the ${\chi}^2$-test. Results: Eighty-eight point eight percent of nurses and 76.1% of cancer patients thought the patients needed the neutropenic diets. The most important decisional criteria to determine dietary restriction was neutrophil count for nurses and food type for patients. The two groups showed significantly different allowance to raw fruits and raw vegetables, sterilized canned juice, fried vegetables, yogurt, uncooked tofu, salted fish, cooked fish, cooked shellfish, uncooked grain powder, jellified food, home-made bread/cookies, nuts including peanuts, instant coffee or tea and tea brewed. In general, patients were more permissive about the neutropenic diet than nurses. Conclusion: It is recommended to consider patients' preference as well as nurses' professional knowledge and publish standardized clinical diet guidelines for neutropenic patients with collaboration between nurses and patient representatives.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
• /
• 2004.04a
• /
• pp.227-232
• /
• 2004

Ballast water from ship harmful microorganism sterilized use of electrolytic sterilization system. The experimental methods were use of peristaltic flow pump upward on electrode pole. Due to reaction time, HRT were unlike microorganism on flow rate. In electrolysis, dioxide iridium coated titanium(Ti／IrO$_2$) and stainless steel plate were used for anode and cathode respectively. Current density controls make use of D.C Power supply on 250V 100Amper. Experimental use of current density between 0.1 and 0.5A was able to disinfect microorganism at 5 seconds by the reaction time. This study shows that the electrolyzed water has a potential for the sterilization of ballast water.

• Journal of Nutrition and Health
• /
• v.29 no.9
• /
• pp.1042-1048
• /
• 1996

In order to investigate heating rate of commercial milk products in korean market, lactulose content and availability of calcium and ascorbic acid were measured. The pH range for raw and commercial milk was 6.5-6.75, and protein content was 2.5-3.13%. The acid degree value (。SH)showed between 5.3 and 7.1. There were no differences in pH, protein and acid degree among 3 types of milk. The portion ionic Ca content in UHT-milk(32.4%) and pasteurized milk (27%) increased significantly in comparison with raw milk (4.6%). Pasteurization led to loss of 20% ascorbic acid, and losses of UHT-treatment are approximately twice as high. Significant differences of lactulose content between UHT milk and pasteurized milk were observed. Some of UHT milk products showed very high value of lactulose content like sterilized milk. It is well known that the stress caused by indirect UHT treatment is slightly higher compared with the direct process. The results in this experiment suggested that most of UHT milk in korean market may be treated by indirect UHT method. In oder to keep the adventages of milk component, the heating methods of milk have to be reconsidered.

• The Korean Journal of Mycology
• /
• v.44 no.1
• /
• pp.51-56
• /
• 2016

Diverse endophytic fungi were isolated from surface-sterilized leaves of three species of conifers inhabiting various sites in Korea: Abies nephrolepis, Pinus koraiensis and Taxus cuspidate. The isolates were identified based on morphological characteristics and sequences analysis of both internal transcribed spacer and large subunit regions of rDNA. In this paper, we report on five previously unreported species of endophytic fungi isolated from conifers: Biscogniauxia maritime, Nemania diffusa, Pezicula carpinea, Phomopsis juglandina and Sydowia polyspora.

• Proceedings of the KWS Conference
• /
• 2002.10a
• /
• pp.133-138
• /
• 2002

Microbiologically Influenced Corrosion (MIC) was suspected in a corrosion failure of cooling system of stainless piping welded joint, carrying marine water. Marine water which is used for cooling water in a plant was transferred to the laboratory and used for experiment. In the first experiment, weld metal samples were exposed to the test solution for 56 days (marine water and sterilized marine water (control)). Surface condition of experimental coupons was observed using a Scanning Electron Microscope (SEM). In another experiment, free corrosion potential of these material was monitored for 56 days. Pitting corrosion was found in the coupons exposed to marine water. Free corrosion potential ennoblement was found to be significant compared to control. It was suspected that this corrosion case was MIC. In the second experiment, coupons were exposed to diluted nutrient medium containing single culture of microbes isolated from the MIC causing marine water sample used for the first experiment. After exposure test, surface condition of experimental coupon was observed using SEM. Pitting corrosion was found in coupons exposed to some of the isolates. The results indicate that they contribute to the corrosive effect of the SUS316L welds.

• Journal of Korean Society on Water Environment
• /
• v.20 no.1
• /
• pp.32-36
• /
• 2004

In this study, uptake and translocation of 2,4,6-trinitrotoluene(TNT) by plant in a hydroponic culture was quantified with two indigenous plant species, Aeschynomene indica and Abutilon avicennae on various initial concentrations of TNT ranging from 20 to 80 mgTNT/L. Experiments were sterilized to exclude the activity of microorganisms and conducted in duplicate. Weight loss of two plant species in added TNT culture media was higher than in control. At over 2OmgTNTIL, there appeared to be phytotoxicity from TNT as indicated by severe yellow-chlorosis and increase of falling leaves. TNT removal rate normalized(K) to the plant fresh weight of Abutilon avicennae and Aeschynomene indica was that the higher TNT concentrations resulted in lower TNT removal rate normalized(K) to the plant fresh weight. Approximately 96% of the TNT in viable microflora-hydroponic culture was removed after 96h of the experiments.

• Food Science and Preservation
• /
• v.7 no.2
• /
• pp.222-227
• /
• 2000

Antimutagenic activity of crushed kimchi fermented with starter under various conditions such as temperature(5-20$^{\circ}C$), salt concentration(2-10%), addition rate of starter (0-20%) was investigated. The kimchi was fermented with crushed Chinese cabbage without salting, red pepper powder, crushed garlic, crushed ginger, anchovy juice and starter. Well fermented kimchi juice(fermented at 10$^{\circ}C$ for 15 days) and sterilized radish juice was used as a source of lactic acid bacteria and starter medium, respectively. Antimutagenic activity showed the highest in the crushed kimchi fermented at 15$^{\circ}C$ for 15 days, 4% salt concentration, 5% starter added, respectively. The inhibition rate of mutagenic activity of the kimchi against S. typhimurium TA98 induced by NQO and S. typhimurium TA100 induced by MNNG was 56.41% and 60.11%, respectively. And the inhibition rate of the kimchi juice showed 56-60% per 100ul.

• Asian Pacific Journal of Cancer Prevention
• /
• v.16 no.2
• /
• pp.831-836
• /
• 2015

Tumor fluid accumulation occurs in both human cancer and experimental tumor models. Solid tumors show a tendency to tumor fluid accumulation because of their anatomical and physiological features and this may be influenced by molecular factors. Fluid accumulation in the peri-tumor area also occurs in the Dunning model of rat prostate cancer as the tumor grows. In this study, the effects of tumor fluids that were obtained from Dunning prostate tumor-bearing Copenhagen rats on the strongly metastatic MAT-LyLu cell line were investigatedby examining the cell's migration and tumor fluid's toxicity and the kinetic parameters such as cell proliferation, mitotic index, and labelling index. In this research, tumor fluids were obtained from rats injected with $2{\times}10^5$ MAT-LyLu cells and treated with saline solution, and 200 nM tetrodotoxin (TTX), highly specific sodium channel blocker was used. Sterilized tumor fluids were added to medium of MAT-LyLu cells with the proportion of 20% in vitro. Consequently, it was demonstrated that Dunning rat prostate tumor fluid significantly inhibited proliferation (up to 50%), mitotic index, and labeling index of MAT-LyLu cells (up to 75%) (p<0.05) but stimulated the motility of the cells in vitro.

• Journal of Forest and Environmental Science
• /
• v.34 no.5
• /
• pp.388-394
• /
• 2018

An efficient and reproducible protocol for in vitro germination of seeds of Couroupita guianensis has been developed in the present study. Couroupita guianensis is a medicinally important tree and categorized as threatened plant species by the IUCN. The seeds were surface sterilized using 0.1% $HgCl_2$ and inoculated on MS (Murashige and Skoog) and Woody Plants (Llyod and McCown) medium. The seeds with partially removed seed coat responded better (100%) on half strength MS medium as compared to the full (51%), one-fourth strength MS (79%) and the WP medium. Half strength MS medium conjunct with $2.0mg{\cdot}L^{-1}$ indole-3 butyric acid (IBA) was found best among the different types of auxins and cytokinins tested for seed germination, as all the seeds germinated on this medium combination. The seedlings were successfully hardened in the greenhouse and restored in the field with 83% survival rate. The protocol can be used as conservation measure for large scale multiplication of this threatened forest tree species.