• Title/Summary/Keyword: sterilize

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Sterilization of Bacteria and Fungi in Cultural Heritages using Atmospheric Pressure Plasma Jet System (대기압 플라즈마 제트 시스템을 이용한 문화재 내 세균류 및 진균류의 살균)

  • Jo, Sung-Il;Park, Dong-Min;Lee, Byeong Hoon;So, Myoung-Gi;Ha, Suk-Jin;Jeong, Goo-Hwan
    • Journal of the Korean institute of surface engineering
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    • v.53 no.6
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    • pp.285-292
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    • 2020
  • Ancient cultural heritage made up of wood and organic fibers have been easily disintegrated or decomposed by various microorganisms like bacteria and fungi. Here, we demonstrate the effectiveness of an atmospheric pressure plasma jet (APPJ) system to sterilize the microorganisms in tangible cultural heritage. We collected several specimens from the surface of ancient documents and wooden artifacts. Finally, two bacteria and two fungi were prepared and sterilized using the APPJ treatment. The APPJ system is beneficial to its simple apparatus, quick operation time, and cost-effectiveness. Bacteria were almost sterilized within only 1 min treatment using 15 % O2 and applied bias voltage of 100 V. In case of the fungi, sterilization rate reached over 83 % but difficult to reach over 90 % even 10 min treatment. According to the plasma diagnostics using optical emission spectroscopy, it was found that the reactive oxygen species such as OH groups are critical for sterilization of microorganisms. Although further efforts should be performed, we believe that efficient sterilization could be realized by the simple, quick, and portable APPJ treatment system.

Leslie Marmon Silko's Decolonizing Efforts and Syncretic Vision in Gardens in the Dunes (『모래언덕 위의 정원』에 나타난 레슬리 마몬 실코의 탈식민화 작업과 혼합주의적 비전)

  • Kang, Ja Mo
    • Journal of English Language & Literature
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    • v.55 no.4
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    • pp.597-618
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    • 2009
  • Leslie Marmon Silko, in her novel Gardens in the Dunes, primarily focuses on revealing the white colonialists' plan to exterminate and destroy American Indians and their culture. In this regard, this novel is clearly an Indian counter narrative to interrogate and abrogate the authority of the oppressive and destructive discourse of the whites who are full of colonialist impulses to sterilize Indians and their culture. However, it should be noted that Silko is very careful not to insist on cultural exclusivism and reverse ethnocentrism, since these only mean a return to the violent colonialists' discourse based on dualism and cultural authenticity which, she believes, has led to the marginalization and eventual deterioration of Indians and their culture. White values and culture are something to recognize and tolerate as long as they are not the products of witchery, also known as the destroyer or evil for Silko, which promotes disruption and antagonism between races and classes. As she reveals in her interview, her major concern in the novel is to dismantle political and/or racial distinctions like Native Americans versus EuroAmericans and thus to enhance the idea of the reconciliation and coexistence of whites and Indians. Silko's Gardens in the Dunes can be regarded as an experiment in the possibility of the universal and homogeneous (at least in its roots) global culture which tolerates all forms of culture. Global culture does not mean a uniform totalitarian culture but a vision of a harmonious world characterized by hybridity and heterogeneity, in which different cultures associate freely without the notion of inferiority or superiority of any one culture. Silko's belief in syncretism emphasizes the spirit of tolerance and exchange between different cultures, dismantling the authority of exclusive ethnocentrism. The ultimate message implied in Gardens in the Dunes is that the syncretic spirit is not only an effective means to correct the white colonialists' hegemonic desire aimed at the extermination of Indians and their culture but also a source of energy for the life and prosperity of modern Indians and their societies.

Microbial Growth in Dried Fishes During Preservation (건어물 저장 중 미생물 증식상태에 관한 연구)

  • 이현자;김종군;이수정;조한옥
    • Journal of Food Hygiene and Safety
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    • v.8 no.3
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    • pp.135-140
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    • 1993
  • Dried fishes such as dried pollack, dried sliced squid, dried white bait, dried anchoy) and dried cod used to cook Korean traditional foods were airpacked in polyethylene tube and irradiated with 7 kGy of gamma-ray source. The effect of gamma irradiation on microbial growth in dried fishes was investigated during storage at ambient temperature for 12 months. The total aerobic bacteria in the control group were contaminated by $3.9{\times}10^{3}\;cells/g$ in dried pollack, $5.6{\times}10^{5}\;cells/g$ in dried sliced squid, $1.2{\times}10^{5}\;cells/g$ in dried white bait, $1.2{\times}10^{4}\;cells/g$ in dried anchovy and $1.2{\times}10^{4}\;cells/g$ in dried cod and 7 kGy irradiation could eliminate the bacteria and also reduce aerobic bacterial load to 1~3 log cycle and no apparent growth of microorganisms occurred during storage. Molds in the control group were contaminated by $4.0{\times}10^{2}\;cells/g$ in dried pollack, $$1.3{\times}10^{2}\;cells/g$ in dried sliced squid, $2.5{\times}10^{2}\;cells/g$ in dried white bait, 90 cells/g in dried anchovy and $2.0{\times}10^{2}\;cells/g$ in dried cod, respectively. 7 kGy irradiation could sterilize the molds and the growth of molds of nonirradiated samples were slightly decreased during storage. Yeasts in the control group were contaminated by $1.4{\times}10^{3}\;cells/g$ in dried poUack, 75 cells/g in dried sliced squid, $1.1{\times}10^{3}\;cells/g$ in dried white bait, 50 cells/g in dried anchovy, $1.6{\times}10^{2}\;cells/g$ in dried cod, respectively and irradiation could sterilize the yeasts and growth of yeasts was slightly decreased in dried pollack and dried white bait but increased in order of dried anchovy, dried cod and dried sliced squid. Coliforms in all sample were sterilized by irradiation and its growth was decreased during storage except dried anchovy.

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Effect of the Nematode Sterilization of Nursery Medium using QRD Microwave in the Plant Factory (QRD 마이크로파를 이용한 식물공장용 배지의 멸균 효과)

  • Kim, Jin Hyun;Kim, Tae Wook;Lee, Keun Woo;Ha, Yu Shin;Lee, Jae Hyun;Kim, Kyung Min
    • Journal of Bio-Environment Control
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    • v.22 no.2
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    • pp.100-107
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    • 2013
  • Unlike general microwave, QRD (Quadratic Residue Diffusor) Microwave used in this study is known as a new technology that enhances the sterilization effect with low power because it is possible to induce the average sterilization by changing wavelength phase difference. Therefore, basic research was conducted on the function that could sterilize culture media for plant factory by using environmentally friendly and low energy consuming QRD Microwave. The results are as follows: It was confirmed that there was no external deformation in the polyurethane foam and rock wool medium when changing the microwave level between 2 and 8 kW in different water content of culture media. However, PDA solid media at 2 kW were not dissolved in 60 and 180 seconds. All of the media were dissolved in other processing. There was little difference in the microwave irradiation level and surface temperature of the strain according to the processing time between Bacillus sp. and Burkholderia sp. In the sterility test according to the microwave irradiation level and processing time, it was confirmed that both Bacillus sp. and Burkholderia sp. grew in the microwave level 2 kW regardless of time. In the microwave level 6 kW, all experimental groups except the processing of Burkholderia sp. for 60 seconds were sterilized, and all of Bacillus sp. was killed in the all experimental groups. In the microwave level 8 kW, it was confirmed that both Bacillus sp. and Burkholderia sp. were sterilized regardless of time. The temperature in microwave-processed media after contaminating strains to each medium was maintained at more than 100 in polyurethane foam and rock wool medium after 60 seconds. In general, it was shown that it was possible to sterilize after 60 seconds. Therefore, it is considered that Bacillus sp. and Burkholderia sp. which are the biggest problems in the plant factory can be adequately sterilized by QRD Microwave used in this study.

Development of a Pre-treating Equipment and the Carcass Disposal System for Infected Poultry (감염가금 전처리 및 폐사가축 처리시스템 개발)

  • Hong, J.T.;Kim, H.J.;Yu, B.K.;Lee, S.H.;Hyun, C.S.;Ryu, I.S.;Oh, K.Y.;Kim, S.;Kwon, J.H.;Tack, D.S.
    • Journal of Animal Environmental Science
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    • v.17 no.2
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    • pp.81-92
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    • 2011
  • When we bury the infected poultry into the ground, we have many problems such as the difficulty of making sufficient area for burying, environmental contamination by the leachate, unpleasant ordor. Also, in case of burning the carcass of the infected poultry, there are some problems such as high cost, dust, unpleasant odor, etc. It could cause environmental contamination which many peoples and environmental organization complains about. In this study, we develop a treating system which treats the infected poultry carcass in a environmental method preventing the environment contamination. This system is composed of many processes. The euthanasia system uses rigid vinyl to trap and to do a euthanasia the infected poultry with lethal gas, carbon dioxide. And then, with the tractor attached grappler infected poultry carcass could be put into the carcass treating system. The euthanasia system uses rigid vinyl to trap the infected birds and to confine lethal gas, carbon dioxide. Infected poultry carcass are moved to carcass disposal system by collecting device which is attached at tractor. The carcass treatment system (capacity of disposal : 6.3 $m^3$) is installed on a truck and do one pass work, which is input, crush, stir, sterilize, and discharge treated carcass. 1,000 chickens was killed within 9.7min by $CO_2$ (300L/min) in the tent (10 $m^3$). The collecting device could carry 142 chickens at a time, and the movable carcass treatment system could sterilize 2 tons carcass per hour (at one time). This treatment systems was eco-friendly because it reduced the volume of carcass by 31.9% with no wastewater generation.

Effect on Corrosion Characteristics of SS 400 Steel by Alkali Water pH from Electrolysis of City Water (수돗물의 전기분해에 의해서 생성된 알카리수의 pH가 SS 400강의 부식특성에 미치는 영향)

  • Moon, Kyung-Man;Ryoo, Hae-Jeon;Kim, Yun-Hae;Jeong, Jae-Hyun;Baek, Tae-Sil
    • Journal of Ocean Engineering and Technology
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    • v.31 no.3
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    • pp.248-255
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    • 2017
  • Many rivers and seas have been affected by environmental contamination. Therefore, city water supplies often require a high-degree purification treatment to provide safe drinking water. However, in order to achieve a high-degree purification treatment, a large amount of chlorine has to be added to sterilize city drinking water. The added chlorine reacts chemically with water and forms hypochlorous and chlorine ions. The hypochlorous ionizes with hypochlorous ions and hydrogen ions. As a result, the city water contains a large amount of chlorine ion. As such, when city water is used with domestic boilers, many kinds of heat exchangers, and the engines of vehicle and ships, there are often corrosion problems. In this study, alkali water was electrochemically made by electrolysis of city water, and corrosion properties between alkali and city water were investigated with an electrochemical method. Most of the chlorine ions are thought to not be contained in the alkali water because the alkali water is created in the cathodic chamber with an electrolysis process. In other words, the chlorine ion can be mostly removed by its migration from a cathodic chamber to an anodic chamber. Moreover, the alkali water also contains a large amount of hydroxide ion. The alkali water indicated relatively good corrosion resistance compared to the city water and the city water exhibited a local corrosion pattern due to the chlorine ion created by a high-degree purification treatment. In contrast, the alkali water showed a general corrosion pattern. Consequently, alkali water can be used with cooling water to inhibit local corrosion by chlorine ions in domestic boilers, various heat exchangers and the engine of ships and for structural steel in a marine structure.

Design of a shoe rack for effective sterilization and deodorization of the shoes contaminated by various bacteria (오염된 신발의 효율적인 살균과 탈취를 위한 신발장의 설계)

  • Lee, Sam-Cheol;Jang, Yong-Seok
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.6
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    • pp.199-206
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    • 2016
  • The aim of this study was to establish the optimal conditions to design a shoe rack that could effectively sterilize and deodorize shoes contaminated by various bacteria. The manufacture and evaluation of a shoe rock was conducted for one year from October 2014 to September 2015. The antibacterial efficacies were evaluated using Escherichia coli and Staphylococcus aureus by JIS Z 2801:2010. The deodorization efficiency was identified by the standard method for deodorization efficiency evaluation of an indoor air cleaner established by Korea Air Cleaning Association, SPS-KACA002-132:2006. The results showed that antibacterial evaluation showed more than 99.9% of the sterilization effects on Escherichia coli and Staphylococcus aureus after exposure to ultraviolet (UV) light for 30 seconds. The average deodorization efficiency for the test gases was 42.5%. The temperature in the shoes and in the shoe rack was lower than $40^{\circ}C$ and $25^{\circ}C$, respectively, when the UV lamp was turned on for 5 minutes and off for 25 minutes repeatedly. This was controlled by turning the UV lamp on and off to maintain the appropriate temperature in the shoes. In conclusion, the real deodorization efficiency is expected to be higher than the measured value because the internal volume of the shoe rack was smaller than the test chamber used to measure the deodorization efficiency. The deodorization effect could be increased further by the sterilization of bacteria causing bad odors in shoes. Further studies will be needed to determine the temperature deviations within the shoe rack to provide constant conditions.

Effect of Drying Methods of Rice Flour on Growth Properties of Bacillus cereus and Enterobacter sakazakii (쌀가루의 건조방법에 따른 Bacillus cereus와 Enterobacter sakazakii 생육 억제 특성)

  • Choi, Bong-Kyu;Park, Shin-Young;Ha, Sang-Do;Kum, Jun-Seok;Lee, Hyun-Yu;Park, Jong-Dae
    • Korean Journal of Food Science and Technology
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    • v.39 no.3
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    • pp.295-298
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    • 2007
  • In order to sterilize Bacillus cereus and Enterobacter sakazakii in rice flour, hot-air drying ($65^{\circ}C$/15 min, HT) and microwave drying (700 watt/30 sec, MT) treatments were evaluated and a storage study performed. Color changes (${\Delta}E$) appeared to be less in the MT rice flour than in HT treated rice flour. The effectiveness of the MT treatment showed reduced growth rates for B. cereus (0.54 log CFU/g) and E. sakazakii (1.45 log CFU/g). The populations of B. cereus in the control (NT) rice flour greatly increased during storage at 4, 10 and $20^{\circ}C$ as storage times increased. However, the growth of B. cereus was minimized in the MT rice flour. In conclusion, MT treatment is considered to be a good drying method when substituted for HT treatment to assure microbial safety in rice flour.

Oral Health Awareness According to Actual Condition of Oral Hygiene Products Management of College Students (대학생의 구강위생용품 관리실태에 따른 구강건강인식도)

  • Oh, Na-Rae;Jeong, Mi-Ae
    • The Journal of the Korea Contents Association
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    • v.18 no.7
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    • pp.313-321
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    • 2018
  • This study was conducted to investigate the oral health perception according to the actual condition of oral hygiene products management in 222 college students in Daegu and Kyungbuk province (p<0.05). The subjects in the study were 43 men (19.4%) and 50 women (22.5%) with two toothbrushes (p<0.05), and the major subjects were 2 tooth brushes The subjects were 18 (8.1%) and 75 subjects (33.8%) were subjects of science major (p<0.001). Among the subjects who did not carry a toothbrush, 72(32.4%) were male and 46 (20.7%) were female. The number of subjects who did not carry the male was higher (p<0.05) The subjects were as follows: 83 (37.4%), and 21. Oral health awareness was 3.031 times higher than that of male (p<0.001), and the major group was 2.57 times higher than that of the general population (p<0.001). Subjects who did not sterilize the toothbrush had 0.459 times lower oral health perception (p<0.05). In order to establish and evaluate the oral health policy of university students in Korea, only the objective oral health status is used as an index. However, it is necessary to study and reflect the variables affecting oral health awareness as much as the oral health awareness reflects not only oral health condition but also socioeconomic variables and the effect of variables on oral health.

Effects of High Hydrostatic Pressure and Gamma Irradiation on Quality and Microbiological Changes of Kochujang-Gulbi. (고추장굴비의 품질과 저장성에 미치는 초고압처리와 감마선 조사 효과)

  • Kang, Seong-Gook;Park, Nan-Hee;Ko, Do-Ock;Li, Jing-Lei;Kim, Bo-Sub;Park, Yang-Kyun
    • Food Science and Preservation
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    • v.18 no.1
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    • pp.1-6
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    • 2011
  • Kochujang-gulbi, a Korean traditional food, was prepared with kochujang and freeze-dried gulbi slices. Kochujang-gulbi was treated with high hydrostatic pressure (200, 400 and 600 MPa) and gamma-irradiation (7, 10, 20 and 30 KGy) to improve its quality and shelf-life. The pH of high hydrostatic pressure and gamma-irradiation treated kochujang-gulbi samples did not significantly different compared to that of control. However, Hunter L value slightly increased, and Hunter a and b values decreased by high hydrostatic pressure and gamma-irradiation treatment. During storage, the pH and color of high hydrostatic pressure and gamma-irradiation treated kochujang-gulbi samples did not significantly changed. The VBN and TBA level decreased by the increase treatment of high hydrostatic pressure and then slowely increased during storage. The VBN and TBA level of kochujang-gulbi samples treated by the strong gamma ray emission slowely increased during storage. In particular, gamma-irradiation treatment was very effective to sterilize microorganisms when compared to that of high hydrostatic pressure in kochujang-gulbi products. In addition, the high hydrostatic pressure and gamma-irradiation treated kochujang-gulbi samples shown significantly lower total viable cell number than control for the 20 days of storage at room temperature with retort pouch packaging.