• BMB Reports
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• v.31 no.3
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• pp.254-257
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• 1998

The gshI gene from the Escherichia coli K-12 strain codes for ${\gamma}-glutamylcysteine$ synthetase which mediates the rate-limiting step of glutathione biosynthesis. The isolated gshI gene from E. coli K-12 has an unusual translation initiation codon, UUG. The 494th amino acid is Ala rather than Gly which was found in a mutant strain E. coli B. In order to improve the translational rate of the gshI gene of E. coli K-12, the initiation codon, UUG, was changed to the usual AUG codon by the site-specific mutagenesis. This change has resulted in a 53% increase of ${\gamma}-glutamylcysteine$ synthetase activity. The enzyme activity was also improved by replacing $Ala^{494}$ with Val (A494V) or Leu (A494L). The replacement of $Ser^{495}$ with Thr (S495T) also resulted in a 62% increase of the enzyme activity. Therefore, the specific activity of ${\gamma}-glutamylcysteine$ synthetase was increased with the increasing chain length of the aliphathic amino acid at the site of the 494th amino acid (Ala<$Val{\leq}Leu$).

• Journal of applied mathematics & informatics
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• v.8 no.1
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• pp.181-197
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• 2001

A heuristic method TABU-CSP using Tabu Search (TS) is described for solving Constraint Satisfaction Problems (CSPs). The method is started with a complete but inconsistent solution of a binary CSP and obtained in prespecified number of iterations either a consistent solution or a near optimal solution with an acceptable number of conflicts. The repair in the solution at each iterative step is done by using two heuristics alternatively. The first heuristic is a min-conflicts heuristic that chooses a variable with the maximum number of conflicts and reassigns it the value which leads to the minimum number of conflicts. If the acceptable solution is not reached after the search continued for a certain number of iterations, the min-conflict heuristic is changed and the variable selected least number of times is chosen for repair. If an acceptable solution is not reached, the method switches back to the min-conflict heuristic and proceeds further. This allowed the method to explore a different region of search space space for the solution as well as to prevent cycling. The demonstration of the method is shown on a toy problem [9]which has no solution. The method is then tested on various randomly generated CSPs with different starting solutions. The performance of the proposed method in terms of the average number of consistency is checked and the average number of conflicts is conflicts is compared with that of the Branch and Bound(BB) method used to obtain the same solution. In almost all cases, the proposed method moves faster to the acceptable solution than BB.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.19 no.9
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• pp.93-99
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• 2020

Recently, as the weight reduction of vehicles has been actively progressed, parts developed using aluminum 60XX series from existing steel materials are increasing. In this paper, the bushing used for the front frame rail, which is one of the parts for fixing engines and other parts in automobiles, was changed to an aluminum material of the Al60XX series, and it was intended to be produced by applying of cold forging method. The bushing is a part that secures the engine frame, and in order to produce it by cold forging, the molding limit is predicted through process design, and a multi-stage process is designed through finite element analysis. In addition, in order to verify the feasibility of the designed forging process, the limits of the multi-step process were verified based on the Cockcroft Latham theory, and the crack and overlap of the actual forging work were predicted and improved.

• Transactions of the Korean Society of Automotive Engineers
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• v.6 no.5
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• pp.211-221
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• 1998

Thermal behavior on the cylinder block of a 4-cylinder, 4-stroke 2.0L SOHC gasoline engine was numerically and experimentally analyzed. The numerical calculation was performed using the finite element method. The cylinder block was modelled as a three dimensional finite element by considering its geometry. The physical domain was devided into hexahedron elements. 16 thermocouples were installed at points of 2mm inside from cylinder wall near top ring of piston in cylinder block, which points have suffered major thermal loads and suggested as proper measurement points for engine design by industrial engineers. Under full load and 9$0^{\circ}C$ coolant temperature condition, temperature behavior of cylinder block according to engine speed were analyzed. The results showed that temperature rose gradually to conform to a function of 2nd~4th order of engine speed at intake side, exhaust and siamese side, respectively. As engine load was changed from 100 to 50% by 25% step, temperature curve also conformed to 2nd~7th order function of engine speed. Temperature differences by load condition were similar among 100, 75% and 50%. Under full load and coolant temperature of 11$0^{\circ}C$, temperature behavior were also analyzed and the result also showed conformance to 2n d~7th order function of engine speed. Temperature curve was transferred in parallel upwards corresponding coolant temperature rise.

• Korean Journal of Biological Psychiatry
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• v.4 no.1
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• pp.136-145
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• 1997

We are report on three cases of typical clinical characterstics and treatment response in neuroleptic maligant syndrome(NMS), and reviewed the literatures of NMS. NMS was first recognized as a life-threatening complication of dopamine receptor antagonists, and defined as a catatonic-like states associated with fever, obtundation, muscle rigidity, and unstable vital sign in patients taking neuroleptic agents. Concepts of NMS have changed because medications other than classic neuroleptic drugs have been implicated as triggering agents and syndromes identical to NMS have been observed in other conditions. The important neurochemical features are probably functional dopamine deficiency and ensuing hyperactivity of excitatory amino acid neurotransmission in the basal ganglia and hypothalamus. Recognition of NMS and early discontinuation of neuroleptics are the most important step in its management. Supportive care includes management of hyperthermia and fluid replacement. Controversial therapeutic measures include the application of dopamine receptor agonists, excitatory amino acid antagonists, or dantrolene. Psychiatric patients with a history on NMS and psychotic relapse necessitating antipsycotics do not commonly redevelop NMS.

• Journal of Korean Society on Water Environment
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• v.26 no.5
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• pp.810-815
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• 2010

Ultrasonic sludge pre-treatment has been studied to enhance the performance of anaerobic digestion by increasing sludge hydrolysis which is regarded as the rate-limiting-step of anaerobic digestion. In this study, the effect of ultrasonic pre-treatment on sludge hydrolysis (solubilization) and methane production was investigated. Sludge solubilization efficiency increased with ultrasonic energy input. However, it is uneconomical to apply more than 720 kJ/L as the solubilization efficiency per energy input declines afterwards. Volatile fatty acids concentration increased after the ultrasonic sludge hydrolysis. Anaerobic batch digestion showed that methane volume reached 64.7 and 84.5 mL after 18 days of incubation with the control sludge and ultrasonically hydrolyzed sludge, respectively. Methane production potential, maximum methane production rate, and the lag time of modified Gompertz equation were changed from 70 mL, 6.4 mL/day, and 1.2 days to 89 mL, 9.6 mL/day, and 0.5 day, respectively, after the ultrasonic sludge treatment. The results proved that ultrasonic pre-treatment contributed significantly not only for the methane production but also for the reduction of anaerobic digestion time which is critical for the performance of anaerobic sludge digestion.

• Korean Journal of Materials Research
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• v.15 no.5
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• pp.348-352
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• 2005

Gallium oxyhydroxide (GaOOH) powders were heat-treated in a flowing ammonia gas to form GaN, and the reaction kinetics of the oxide to nitride was quantitatively determined by X-ray diffraction analysis. GaOOH turned into intermediate mixed phases of $\alpha-\;and\;\beta-Ga_2O_3$, and then single phase of GaN. The reaction time for full conversion $(t_c)$ decreased as the temperature increased. There were two-types of rapid reaction processes with the reaction temperature in the initial stage of nitridation at below $t_c$, and a relatively slow processes followed over $t_c$ does not depends on temperatures. The nitridation process was found to be limited by the rate of an interfacial reaction with the reaction order n value of 1 at $800^{\circ}C$ and by the diffusion-limited reaction with the n of 2 at above $1000^{\circ}C$, respectively, at below $t_c$. The activation energy for the reaction was calculated to be 1.84 eV in the temperature of below $830^{\circ}C$, and decreased to 0.38 eV above $830^{\circ}C$. From the comparative analysis of data, it strongly suggest the rate-controlling step changed from chemical reaction to mass transport above $830^{\circ}C$.

• Korean Institute of Interior Design Journal
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• v.20 no.2
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• pp.72-84
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• 2011

In accordance with an active development, we now enjoy web 2.0 generation centering bilateral participation through the web 1.0 environment mainly providing the offering information and take a step web 3.0 understanding information by itself. The same time of digital technic development, consumption market arrived at market3.0 generation, also passed by market 1.0 and went via market 2.0 based on bilateral participation and understanding. This change have the marketing implement index changed as well. Nowadays, 7C is generalized of making up sensibility, emotion, communication, relationship, customization and interactive built as a major strategy from the day of 3p focused on customer environment, service process and 4P focused on physical quality of product. It isn't that brand and merchandise create life and experience like as past. It is focused on embodiment of a way of life, culture and creative experience from consumers' own. This treatise, therefore, fixes the object as flagship store positively reflecting the change and examine the change of a marketing code element and social factor stimulating customer behavior change. As a condition of this study, It fixes Maslow Motivation Theory as the main index of customer behavior, and 7C as the marketing code. This treatise ultimately studies the index of flagship store discussion based on domestic outdoor brand cases.

• Journal of the Korean Society for Precision Engineering
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• v.20 no.1
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• pp.151-158
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• 2003

Design changes frequently occur while design activities are performed. If the impact of design changes is estimated, design efficiency can be improved. But, the types of design changes are various and they can affect other design parts. Hence, it is difficult to deal with design changes directly. The purpose of this research is to develop systematic algorithms for change propagation tracing and change impact analysis, and then to implement a change impact analysis system. We have selected a process-based design and a design environment which is composed of design parameters and constraints. The algorithm for change propagation tracing tracks the change propagation of design parameters and finds design parameters, constraints and tasks which are probably changed. In the algorithm for change impact analysis, a change impact value is calculated from the list of changeable tasks. These two algorithms have been implemented into change impact analysis system (CIAS). CIAS has been applied to the redesign of 2 stage gear drives. CIAS can improve the efficiency of design activities. If there are many alternatives for a design change at the redesign step, designers can calculate the change impact value of each alternative and perform design change activities in the direction of minimizing design change impact.

• The Journal of Information Technology
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• v.4 no.1
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• pp.9-22
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• 2001

In this paper, We control remote robot using web browser. This system used independent four PC and each PC is used to operator PC, database server, web server and robot control PC. Remote control can escape the operator from constraint the time and workspace. If operator inputting control data in web browse then the control data is saved the data base server. The robot control PC is inputted control data from database at access intervals of 1ms. Also, if the control data is changed then robot is operated. It made simple robot using step-motor and the robot is operated by web browser. Finally, Robot system is exactly operated using control data and certificate of possibility the remote robot control using web browser.