• Title/Summary/Keyword: step by step

Search Result 16,141, Processing Time 0.044 seconds

Cryopreservation of Zona-intact/-free Hamster Oocytes;Effect of 1-Step Equilibration and 2-Step Thawing (투명대 존재/부재 햄스터 난자의 동결보존;1-단계 평형과 2-단계 융해의 효과)

  • Chung, K.M.;Pang, M.G.;Kim, S.H.;Shin, C.J.;Kim, J.G.;Moon, S.Y.;Lee, J.Y.;Chang, Y.S.
    • Clinical and Experimental Reproductive Medicine
    • /
    • v.19 no.2
    • /
    • pp.143-152
    • /
    • 1992
  • The present experiments were focussed to modify a short slow-cooling protocol used for freezing of early stage embryo(Testart et al., 1986) and also to apply the modified method for the cryopreservation of hamster oocytes with Zona or without. The protocol was modified by changing the 4-step equilibration into 1-step and the 1-step thawing into 2-step. The oocytes were added in 1.5M PROH and 0.1M Sucrose, seeded at $-7^{\circ}C$, slow cooled($0.3^{\circ}C$/min) to $-30^{\circ}C$ before plunging to $-196^{\circ}C$. The oocytes were thawed at $23-25^{\circ}C$ air(20sec/150sec) and/or 33-35 water(10sec). The survival of the frozen-thawed oocytes was determined by morphologic criteria and their fertilizing ability was also estimated by Sperm Penetration Assay(SPA) system(Chang et al, 1990) using fertile men semen sample. One-step equilibration showed slightly higher survival rate(83.9% vs. 71.0%) and fertilization rate(83.9% vs. 71.0%) compared with four-step(p>0.05). And two-step thawing(air & water exposing) of oocytes frozen after 1-step equilibration showed significantly higher survival rate(96.3%) than one-step thawing at air(85.2%) or water(65.0%) only(p<0.05). Therefore, by the modified method(l-step equilibration & 2-step thawing), Zona-intact(ZI) and Zona-free(ZF) oocytes were frozen and thawed. ZI-oocytes showed significantly higher survival rate(95.4%, 308/323 vs. 67.6%, 240/355) than ZF-oocytes(P<0.01). But the survival of ZF-oocytes was as high as ZI-oocytes in fourteen of twenty-four replicates. ZI-oocytes was also significantly higher fertilization rate($92.4{\pm}8.9%$ vs. $63.7{\pm}18.5%$) and higher mean number of penetrated sperm($6.2{\pm}4.2$ vs. $3.9{\\pm}3.3$) than ZF-oocytes, but not higher than control(fresh oocytes;$99.3{\pm}2.4%$, $8.4{\pm}4.2$)(P<0.001). Conclusively, this modified method will contribute to freeze effectively the hamster oocytes for simplifing of the logical consideration of performing SPA and also to freeze the human and other animal oocytes.

  • PDF

Step by Step Analysis of Root Canal Instrumentation with ProTaper® (ProTaper를 이용한 근관 형성의 단계별 분석)

  • Kim, Mi-Hee;Huh, Bock;Kim, Hyeon-Cheol;Park, Jeong-Kil
    • Restorative Dentistry and Endodontics
    • /
    • v.31 no.1
    • /
    • pp.50-57
    • /
    • 2006
  • The purpose of this study was to investigate influence of each file step of $ProTaper^{(R)}$ system on canal transportation. Twenty simulated canals were prepared with either engine-driven $ProTaper^{(R)}$ or manual $ProTaper^{(R)}$, Group R-resin blocks were instrumented with rotary $ProTaper^{(R)}$ and group M-resin blocks were instrumented with manual $ProTaper^{(R)}$. Pre-operative resin blocks and post-operative resin blocks after each file step preparation were scanned. Original canal image and the image after using each file step were superimposed for calculation of centering ratio The image after using each file step alld image after using previous file step were superimposed for calculation of the amount of deviation. Measurements were taken horizontally at five different levels (1 2, 3, 4 and 5 mm) from the level of apical foramen. In rotary $ProTaper^{(R)}$ instrumentation group, centering ratio and the amount of deviation of each step at all levels were not significantly different (p>0.05). In manual $ProTaper^{(R)}$ instrumentation group, centering ratio and the amount of deviation of each step at all levels except of 1 mm were not significantly different (p>0.05). At the level of 1 mn, F2 file step had significantly large centering ratio and the amount of deviation (p<0.05). Under the condition of this study, F2 file step of manual ProTaper tended to transport the apical part of the canals than that of rotary $ProTaper^{(R)}$.

Enhanced Two-Step Search Scheme for Rapid and Reliable UWB Signal Acquisition (고속 고신뢰의 UWB 신호 동기획득을 위한 향상된 두 단계 탐색 기법)

  • Kim, Jae-Woon;Yang, Suck-Chel;Shin, Yo-An
    • The Journal of Korean Institute of Communications and Information Sciences
    • /
    • v.30 no.12C
    • /
    • pp.1133-1143
    • /
    • 2005
  • In this paper, we propose an enhanced two-step search scheme for rapid and reliable signal acquisition in UWB systems under multipath channels. The proposed TSS-LS (Two-Step Search scheme with the Linear search based Second step) achieves rapid acquisition performance comparable to the conventional TSS-BS (Two-Step Search scheme with the Bit reversal search based Second step) already proposed by the authors, based on the single-dwell search with two-step thresholds and search windows. However, unlike the TSS-BS which employs the bit reversal search in the second step, the proposed TSS-LS utilizes the linear search in the second step to improve the reliability of signal acquisition. Simulation results with multipath channel models by IEEE 802.15.3a show that the two-step search schemes for the UWB signal acquisition can achieve sig nificant reduction of the required mean acquisition time as compared to general search schemes. In addition, we observe that the proposed TSS-LS achieves quite good bit error rate performance for large signal-to-noise ratios, which is favorably comparable to the case of ideal perfect timing.

Design and Performance Evaluation of Micro Stepping Actuator with a Variable Step Size (가변 스텝 마이크로 액츄에이터의 설계 및 구동특성)

  • Lim, Y.M.;Kim, S.H.;Kwak, Y.K.
    • Journal of the Korean Society for Precision Engineering
    • /
    • v.12 no.7
    • /
    • pp.26-31
    • /
    • 1995
  • A new actuating mechanism suitable for a micro positioning device is developed using piezo-electric elements. The actuator can make a step movement of 0.5 .mu. m up to 3.5 .mu. m. The step size can be adjusted on demand. By repeating this step action, long distance movement is achieved. Precise positioning can be obtained by combining the coarse motion with the maximum step size and fine motion. Two types of fine motion have been proposed for a driving method. Firstly, feedback control bases on PID is applied. The experimental results for the two method are presented.

  • PDF

X-ray Sensitometry by Bootstrap Method Using New Step Wedge (새로운 step wedge의 Bootstrap법에 의한 X선 sensitometry)

  • Ahn, Bong-Seon;Huh, Joon
    • Journal of radiological science and technology
    • /
    • v.14 no.1
    • /
    • pp.45-48
    • /
    • 1991
  • Recently developed new step wedge has a small size as $10{\times}10\;cm^2$. It can use central beam, and is affected little by scattered rays emmitted from the neighboring step wedges. It's characteristics are same as the intensity scale method, and are excellent compared with conventional boot strap methods. Moreover, the method using new step wedge is simple in operation, and the characteristics are excellent. Now, the above method using new step wedge can supercede the distance method.

  • PDF

Fault Detection for Extended Kalman Filter Using a Predictor and Its Application to SDINS (예측필터를 이용한 확장칼만필터 고장검출 및 SDINS에의 적용)

  • Yu, Jae-Jong
    • Journal of the Korea Institute of Military Science and Technology
    • /
    • v.9 no.3
    • /
    • pp.132-140
    • /
    • 2006
  • In this paper, a new fault detection method for the extended Kalman filter, which uses a N-step predictor, is proposed. The N-step predictor performs the only time propagations for N-step intervals without measurement updates and its output is used as a monitoring signal for the fault detection. A consistency between the extended Kalman filter and the N-step predictor is tested to detect a fault. A test statistic is defined by the difference between the extended Kalman filter and the N-step predictor. The proposed method is applied to strapdown inertial navigation system (SDINS). By computer simulation, it is shown that the proposed method detects a fault effectively.

Step Length Estimation on a Slope Using Accelerometers and a Barometer (가속도계와 기압계를 이용한 경사면에서의 보행 거리 추정)

  • Hung, Tran Nhat;Suh, Young Soo
    • Journal of Institute of Control, Robotics and Systems
    • /
    • v.19 no.4
    • /
    • pp.334-340
    • /
    • 2013
  • Using a relationship between step length and accelerometer output, step length can be estimated. In this paper, we propose a new step length estimation algorithm, which can be used both for the plane and the slope by compensating the slope angle. The slope angle is estimated using a barometer and the slope angle is compensated by observing how the slope affects the step length estimation. The proposed algorithm is verified using five adult man walking data, where the average length error is about 3% regardless of the slope.

A study of backward-facing step flow in a rectangular duct (후향계단이 있는 사각덕트 내부의 유동특성 연구)

  • Kim, Sung-Joon;Choi, Byung-Dae
    • Journal of Industrial Technology
    • /
    • v.19
    • /
    • pp.59-65
    • /
    • 1999
  • This study is to analyze turbulent flow over a backward-facing step in a rectangular duct. The side wall effects on the internal flow were determined by varying the aspect ratio(defined as the step span-to-height ratio) from 1 to 20. In the flow behind a backward-facing step, separation, recirculation and redeveloping is occurred frequently. These phenomena appear in a particular variation by varying the aspect ratio. The results show that the aspect ratio has an influence on the velocity and reattachment length. When the AR is increased, the reattachment length is increased. For 6 over aspect ration, the rate of increase is decreased. The length of recirculation in the upper corner is increased, as the increase of aspect ration. It's width is not changed in the variation of aspect ration. The transverse, streamwise and spanwise velocities were decreased along the flow down stream of the step.

  • PDF

A study for the XML schema of Plant Equipments based on Plant STEP for the Electronic Commerce (플랜트기자재 전자상거래를 위한 STEP기반의 기자재제품정보 XML 스키마 연구)

  • Han, Soon-Hung;Choi, Kwang-Hyun;Ahn, Ho-Jun;Park, Chan-Kook
    • Proceedings of the SAREK Conference
    • /
    • 2005.11a
    • /
    • pp.526-532
    • /
    • 2005
  • Representation of product data for exchanging and sharing a plant information was established the International Standard by ISO 10303 (STEP) and ISO 15926, but is not used widely in industrial circles by massiveness of standard and complexity of plant itself Specially, they are embodied to STEP data file(ISO 10303 Parts 21), but STEP file is uncomfortable that a person recognizes, and needs specification creation tool. In this paper, we made the integration schema for control valve through related standards, Also, we suggest the practical use framework for electronic commerce to utilize the XML schema that can represents integrate schema of control valve to use XML that is becoming the latest issue.

  • PDF

Manipulation of Microstructures of in-situ Phosphorus-Doped Poly Silicon Films deposited on Silicon Substrate Using Two Step Growth of Reduced Pressure Chemical Vapor Deposition (감압화학증착의 이단계 성장으로 실리콘 기판 위에 증착한 in-situ 인 도핑 다결정 실리콘 박막의 미세구조 조절)

  • 김홍승;심규환;이승윤;이정용;강진영
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
    • /
    • v.13 no.2
    • /
    • pp.95-100
    • /
    • 2000
  • For the well-controlled growing in-situ heavily phosphorus doped polycrystalline Si films directly on Si wafer by reduced pressure chemical vapor deposition, a study is made of the two step growth. When in-situ heavily phosphorus doped Si films were deposited directly on Si (100) wafer, crystal structure in the film is not unique, that is, the single crystal to polycrystalline phase transition occurs at a certain thickness. However, the well-controlled polycrtstalline Si films deposited by two step growth grew directly on Si wafers. Moreover, the two step growth, which employs crystallization of grew directly on Si wafers. Moreover, the two step growth which employs crystallization of amorphous silicon layer grown at low temperature, reveals crucial advantages in manipulating polycrystal structures of in-situ phosphorous doped silicon.

  • PDF