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Stub Dieback of Carnation Caused by Fusarium graminearum

  • Han, Kyoung-Suk;Park, Jong-Han;Park, Yong-Mun
    • The Plant Pathology Journal
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    • v.17 no.2
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    • pp.101-105
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    • 2001
  • A disease survey on the carnation (Dianthus caryophyllus L.) wilt was conducted during the high temperature period (June through August) and the low temperature period (February through May) in 58 greenhouses of its major cultivation areas, including Pusan, Kimhae, and Changwon in Korea from 1998 to 1999. The disease incidence was averaged 5.4% and 11.9% in the low and high temperature periods, respectively. Severe damage was found in summer with high incidences of around 50% in some greenhouses. Close examination of the symptoms and isolation of the causal agent revealed that there was a new disease different from Fusarium wilt caused by Fusarium oxysporum f. sp. dianthi, which was determined as the stub dieback caused by F. was cetermined as the stub dieback caused by F. graminearum (teleomorph : Gibberella zeae). The stub dieback symptoms involved brown rot of stem that started usually from the portion of cutting without discoloration of inner vascular tissues. Seven out of 38 isolates from the wilted plants were identified as F. graminearum, while the others as F. oxysporum f. sp. dianthi. Mycological characteristics of the stub dieback pathogen including colony color, absence of microconidia, and the shape of macroconidia, were consistent with F. graminearum previously described. This is the first report of the carnation stub dieback in Korea.

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Cytological and Morphological Characterization of Anther Derived Plants from Sweet Pepper (Capsicum annuum L.) cv. 'Special'

  • Shrestha, Surendra Lal;Luitel, Binod Prasad;Lee, Taek Jong;Kang, Won Hee
    • Korean Journal of Breeding Science
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    • v.42 no.5
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    • pp.431-438
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    • 2010
  • Anthers of sweet pepper $F_1$ cultivar 'Special' were cultured on Dumas De Vaulx (C medium), supplemented with $0.1mgL^{-1}$ 2, 4-D and $0.1mg{\cdot}L^{-1}$ kinetin with 3% sucrose, and 0.32% phytagel. The calluses obtained were further sub-cultured on Murashige and Skoog (MS) medium without growth regulators for regeneration. Regenerated plantlets were grown in plastic pots under plastic house and characterized their cytological and morphological characters in spring, 2008. Twenty percent plantlets were identified as haploid plants after chromosome and ploidy analysis. Haploid plants contained 12 chromosomes, high stomatal density with small stomatal length as compared to diploid plants. Stomatal length in haploids was 23.3% smaller than diploids. Haploid plants were characterized as small leaf and petiole size, poor vigor, thin stem and short plant height, short internodes and small flower buds, fruit size and fruit weight as compared to diploid plants and most of the haploid fruits were seedless. SP55, SP62, SP68, SP72 and SP77 are found high yielding double haploids with high total soluble content (8.6, 8.7, 9.2, 9.1 and $9.8^{\circ}Brix$, respectively) and desirable fruit shape, and recommended them to exploit as inbred lines for heterosis breeding.

Clinical evaluation of autologous fat graft for facial deformity: a case series study

  • Khorasani, Mansour;Janbaz, Pejman
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.47 no.4
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    • pp.286-290
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    • 2021
  • Objectives: The use of fat grafts in maxillofacial sculpturing is currently a common technique. Unlike fillers, autologous fats unite with facial tissues, but long-term results may still be unsatisfactory. Sharing long-term follow-ups can be helpful in making outcomes more predictable. Materials and Methods: The data from patients who were admitted from 2014 to 2016 for fat augmentation were collected. In all cases, fat grafts were injected by blunt cannula using a tunneling technique in different planes. A fan shape order for the malar, periorbital, nasolabial fold, mandibular angle and body, and perioral area was established. Results: Autologous fat was used for different sites of the maxillofacial regions. Of 15 patients, two patients were not satisfied due to fat graft resorption. For this, further injections were performed six months after the first injection using preserved fat grafts. One patient continued to be dissatisfied. There were no other complications related to fat transplants. Conclusion: Fat transplantation is a safe, reliable, and non-invasive method for facial contour and facial soft tissue defect restoration. Additional methods such as mesenchymal stem cells along with fat injection increase the survival rate of transferred fat.

Platen Weight Reduction Design of Extruder Using Topology Optimization Design (위상최적설계를 활용한 압출기의 플라텐 경량화 설계)

  • Kim, D.Y.;Kim, J.W.;Lee, J.I.;Jo, A.R.;Lee, S.Y.;Jeong, M.S.;Ko, D.C.;Jang, J.S.
    • Transactions of Materials Processing
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    • v.31 no.5
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    • pp.302-308
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    • 2022
  • In this study, the weight of the platen was reduced using the structural strength analysis and topology optimization design of the extruder by finite element analysis. The main components of the extruder such as the stem and billet, were modeled, and the maximum stress and safety factor were verified through structural strength analysis. Based on the results of the structural strength analysis, the optimal phase that satisfies the limitation given to the design area of the structure and maximizes or minimizes the objective function was obtained through a numerical method. The platen was redesigned with a phase-optimal shape, the weight was reduced by 40% (from the initial weight of 11.1 tons to 6.6 tons), and the maximum stress was 147.49 MPa safety factor of 1.86.

Morphometric analysis of the Daphne kiusiana complex (Thymelaeaceae) using digitized herbarium specimens

  • KIM, Yoon-Su;OH, Sang-Hun
    • Korean Journal of Plant Taxonomy
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    • v.52 no.3
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    • pp.144-155
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    • 2022
  • Daphne kiusiana is an evergreen shrub with dense head-like umbels of white flowers distributed in southern Korea, Japan, China, and Taiwan. Plants in China and Taiwan are recognized as var. atrocaulis by having a dark purple stem, elliptic leaves, and persistent bracts. Recently, plants on Jejudo Island were segregated as a separate species, D. jejudoensis, given their elliptic leaves with an acuminate apex, a long hypanthium and sepals, and a glabrous hypanthium. Morphological variations of three closely related taxa, the D. kiusiana complex, were investigated across the distributional range to clarify the taxonomic delimitation of members of the complex. Twelve characters of the leaf and flower were measured from digitized herbarium specimens using the image analysis program ImageJ and were included in a morphometric analysis, the results of which indicate that the level of variation in the characters is very high. The results of a principal component analysis weakly separated D. jejudoensis from D. kiusiana according to their floral characteristics, such as a longer, glabrous hypanthium, and larger sepals. However, some individuals of D. kiusiana, particularly those from Bigeumdo Island, were included in D. jejudoensis. Recognition of D. kiusiana var. atrocaulis based on the leaf shape was not supported in the analysis, and D. jejudoensis may be recognized as a variety of D. kiusiana. Our morphometric analysis shows that digitized images of herbarium specimens could be useful and an additional method by which to investigate more diverse specimens.

An Outbreak of Gray Mold Caused by Botrytis cinerea on Kenaf(Hibiscus cannabinus L.)

  • Kwon, Jin-Hyeuk;Kang, Dong-Wan;Lee, Seong-Tae;Shim, Chang-Ki;Kim, Min-Jeong;Kim, Jinwoo
    • Journal of agriculture & life science
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    • v.50 no.2
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    • pp.25-32
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    • 2016
  • A severe outbreak of gray mold on kenaf (Hibiscus cannabinus L.) was observed on kenaf grown in the research field of Gyeongsangnam-do Agricultural Research and Extension Services, Jinju, Korea in 2014. Gray mold appeared on young plants as gray-brown velvety mold covering stems and leaves. Infections that girdled the stem caused wilting above the infected area and developed a canker. The casual fungus formed grayish brown colonies on potato dextrose agar. The conidia were one celled, mostly ellipsoid or ovoid in shape, colorless or pale brown in color, and 6-18 × 4-10 ㎛ in size. The conidiophores were 15-32 ㎛ in length. These measurements and taxonomic characteristics were most similar to those of Botrytis. DNA sequencing and phylogenetic analysis of the complete internal transcribed spacer rRNA gene region confirmed that the fungal isolates were indeed Borytis cinerea. Koch's postulates were supported by pathogenicity tests conducted on healthy plants. On the basis of mycological characteristics and pathogenicity test on host plants, the fungus was identified as Botrytis cinerea. To the best of our knowledge, this is the first report of a gray mold caused by B. cinerea on kenaf in Korea.

Phylogeny and Conservation of the Genus Bupleurum in Northeast Asia with Special Reference to B. latissimum, Endemic to Ulleung Island in Korea (울릉도 고유종인 섬시호를 중심으로 동북아시아 시호속 식물의 계통과 보전생물학)

  • Ahn, Jin-Kab;Lee, Hee-Cheon;Kim, Chul-Hwan;Lim, Dong-Ok;Sun, Byooog-Yoon
    • Korean Journal of Environment and Ecology
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    • v.22 no.1
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    • pp.18-34
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    • 2008
  • Based on external morphology, each of five species can be classified into three groups: 1) B. falcatum group (B. falcatum, B. scorzonerifolium), 2) B. euphorbioides group (B. euphorbioides) and 3) B. longiradiatum group (B. longiradiatum, B. latissimum). B. falcatum group has cauline leaves linear or lanceolate in shape and attenuate at base and not surrounding the stem. In contrast, B. longiradiatum group and B. euphorbioides group have cauline leaves ovate, lanceolate or panduriform in shape and auriculate or cordate at base and completely surrounding the stem. The inflorescence is basically compound umbels terminated at the apex of stem. But B. euphorbioides group is small in size and pedicels are rather short and the number of the pedicel is ca. 20. On the other hand, B. longiradiatum and B. falcatum groups are large in size and their pedicels are long and the number of the pedicel is around 10. The pore of pollen aperture of B. longiradiatum and B. latissimum is partially projected or not while those of B. falcatum group and B. euphorbioides is usually remarkably projected. The number of somatic chromosomes was counted as 2n=20 in B. falcatum, 2n=12 in B. scorzonerifolium and B. longiradiatum, and 2n=16 in B. euphorbioides and B. latissimum. Although chromosome numbers of B. euphorbioides and B. latissimum are the same, the two species are not likely to relate because the karyotypes of the two species are different from each other. Based on these observations, it can be concluded that B. latissimum is most closely related to B. longiradiatum. However, molecular data indicated that the species is probably related to B. bicaule distributed in central Siberia. In terms of conservation of B. latissimum, overexploitation by human and grazing by goat are most threatened factors.

Study on X bodies in epidermal cells of Carina generalis infected with a mosaic virus (칸나 모자이크병의 X체에 관한 연구)

  • Lee Chang Un
    • Korean journal of applied entomology
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    • v.15 no.4 s.29
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    • pp.199-204
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    • 1976
  • Canna nosaic X bodies, which do not exist in tissues of the healthy plant and are originating in cells of virus infected Canna (Canna generalis BAILEY) with mosaic symptom, are easily observed under microscope through application of vital staining for 2-3 minutes with $1\%$ eosin of $H_2O$ solution added with slight amount of $CH_3COOH$ and distinguishing with N/5HCl followed by washing to inspect. The result of this experiment is summarized as following: 1) The X bodies are observed not only in epiermal cells of leaf of the mosaic virus infected Canna but in those of leaf sheath, stem, and root also, and it is expected that the X bodies are to exist in the flower cells of the disease infected Canna which were missed in this experiment. 2) Shape and nature of X bodies are not constant; in early stage of the disease development, the X bodies have equal contents and vague contour with their small size and round shape, but along with progress of the disease development they attain granular contents and clear contour with their increasing sige and defining shape in cytoplasm. In case of same individual pant, fully developed X. bodies. are increasing in cytoplasm in propoition to severity of mosaic and nettling of the diseased leaf. 3) The staining character of X bodies to eosin is more dense than that of nuclei; Xbodies are stained light red or red while nuclei are stained yellowish brown or light red. 1) It is assumed to be a result of cytoplasmic concentration around nucleus that X bodies are usunlly developed adjacent to nucleus and they are considered to be a cytoplasmic prodct. 5) Thus, I confirm that X bodies originsting in canna plant cells infected with mosaic virus aye multipling in the alive cells.

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A Taxonomic Review of Phytolacca insularis (Phytolaccaceae) (섬자리공(Phytolacca insularis, 자리공과)의 분류학적 재검토)

  • Chae, Seung-Hee;So, Soonku;Han, Kyeongsuk;Kim, Muyeol;Park, Sang-Hong;Lee, Joongku
    • Korean Journal of Plant Taxonomy
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    • v.37 no.4
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    • pp.431-446
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    • 2007
  • The purpose of this study is to review the taxonomical position of Phytolacca insularis Nakai based on morphological characters and ITS sequences. Phytolacca insularis was similar to P. acinosa in the stem shape, inflorescence, apocarpous, eight pistils, eight stamens, pink anther, and eight fruits. But the unique difference between P. insularis and P. acinosa was leaf size and pistil color. Phytolaccri insularis and P. acinosa have a similar sculpturing patterns as an foveolate and microscabrate in pollen, but they differed from P. americana in having a bigger foveolae size in sculpture pattern. Phytolacca insularis and P. acinosa were similar in the seed size but different from P. americana by having a small size of seed. The seed surface of P. insularis and P. acinosa was similar with a irregularly undulate shape, but its feature of P. americana differed in having the reticulate shape. Phytolacc a insularis had identical ITS sequences with P. acinosa. Phytolacca insularis and P. acinosa have a close relationship in the morphological characters and ITS sequences. The examinations of morphology and ITS sequences suggest that P. insularis be a form of P. acinosa although it has been formerly treated as a distinct species.

Approaching Method for Detecting Vessels in the Korean Waters using the Panchromatic Imagery of IRS-1C Satellite (Panchromatic 위성 자료를 이용한 선박 확인의 접근 기법)

  • Suh, Young-Sang;Choi, Chul-Uong;Lee, Na-Kyung;Kim, Bok-Kee;Jang, Lee-Hyun
    • Journal of the Korean Association of Geographic Information Studies
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    • v.5 no.4
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    • pp.86-92
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    • 2002
  • The feasibility of counting number of small vessels and position in Korean waters using the panchromatic imageries derived from the IRS-1C was tested. The parameters for interpretation of satellite's imageries of small vessels were location(position), size, shape, shadow, tone, texture and pattern, height and depth, situation and association with other vessels. The position of small vessels in the sea without GCP(ground control point) was considered to be inclusive in the satellite imagery with 35 km semi-diameter, denoting rough geographical position of the vessel. The size of vessel was measured by length from stem to stern of the vessel, distinguished by following wave on the surface water. Offshore fishing vessels were separated from merchant ships by their length smaller than 100 m. The shape of vessels on panchromatic imagery of IRS-1C appeared just streamline. In case of clouds which were similar to the shape of small vessels, we were able to distinguish between vessel and cloud by shadow of cloud in the water surface. The tone of sea surface was dark black while small vessel appeared bright white. Small vessel was distinguished from the rough texture of the sea surface and the regular pattern of the waves with white capes when weather was not so good. The situation of the fishing activity was estimated by information of fishing method related to the fishing boat such as the pair trawl in the Yellow Sea.

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