• Journal of Bio-Environment Control
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• v.30 no.1
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• pp.10-18
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• 2021

Acclimatization after grafting and graft-take that in order to produce plug seedlings of high-quality are important plug seedling stage which not reduce the plug seedlings quality before shipment. Appropriate acclimatization environment can not only increase seedling quality before secondary growth period, but also effective in promoting the growth of plug seedlings. This study was conducted to determine the optimal the shading period and shading level for acclimatization of tomato and pepper grafted plug seedlings after graft-take. The tomato and pepper seedlings were used in this experiment, and after graft-take, a tunnel was installed on a bed in glasshouse of venlo type to started shading treatment. The shading levels were 35, 55, 75 and 95%, and the shading periods were 1 and 2 weeks, and non-treatment was set as the control. In the case of tomato grafted plug seedling, plant height, stem diameter, dry weight of root, leaf area were significantly higher at the shading period of 1 week and the shading level was 55%. In the case of pepper grafted plug seedling, plant height, stem diameter, and leaf area were the highest in the shading period of 2 weeks and the shading level was 35%. However, dry weight of root, compactness, and T/R ratio, which seedling quality indicators, were lower than in the shading period of 1 week and the shading level 55%. Therefore, considering the quality of seedlings, it is suggested that shading period of 1 week with shading level of 55% treatment can be suitable to produce high quality grafted plug seedlings of tomato and pepper.

• Journal of Bio-Environment Control
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• v.32 no.1
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• pp.40-47
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• 2023

There are many different types of cultivation in tomatoes for year-round production. One of them, semi-forcing cultivation is characterized by growing seedlings in winter season. If grafted seedlings are used in winter season that energy cost can be reduced, because they have tolerance to cold stress. This study was conducted to analyze the rootstock performance by measuring the growth, yield, and leaf-macronutrient content of cherry tomatoes grown in semi-forcing hydroponics. Three domestic rootstocks 'HSF4', '21LM', '21A701', and a control cultivar 'B-blocking' were grafted onto jujube-shaped cherry tomato (Lycopersicon esculentum L.) commercial cultivar 'Nonari'. The total yield per plant with grafted cherry tomato '21A701' was 3,387g, which was 11%, 22% and 24% higher than the yield with 'B-blocking', non-grafted one and 'HSF4'. The stem diameter of '21A701' was thick with 8.26mm, whereas non-grafted one was thin with 7.23mm at 160 days after transplanting. The flowering position of '21LM' was 34% and 47% higher than the flowering position of 'B-blocking' and non-grafted one at 153 days after transplanting. The NO₃-N concentration in petiole sap of '21LM' was the highest with 1,746mg·L^-1 and non-grafted one and 'HSF4' were the lowest with 1,252mg·L^-1 and 1,245mg·L^-1 at 167 days after transplanting. The results indicated that rootstock/scion combinations in cherry tomatoes can affect the plant growth, yield, and the concentration of different NO₃-N in leaves at the late growth stage. Both '21A701' and '21LM' have vigorous root system, which influence the growth and yield increased.

• Korean Journal of Weed Science
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• v.11 no.3
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• pp.174-177
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• 1991

This study was conducted to determine the influence of dithiopyr on growth of transplanted rice with different transplanting depths and the amount of $^{14}C$-dithiopyr adsorpted in the root and shoot of rice plants under paddy soil conditions. The growth rate of transplanted rice was lower in 0 and 0.25cm of transplanting depths with exposed basal stem than in 1 and 2cm of the depths in control plot. In the growth of transplanted rice treated with dithiopyr, plant hight and dry weight were significantly inhibited in 0 and 0.25cm depth plots but not affected.in 0.5, 1, 2 and 4cm depth plots, and roop length were influenced in 0, 0.25 and 4cm depth plots but not in 0.5, 1 and 2cm depth plots. The amount of ratioactivity in shoot and root of rice plants as affected by $^{14}C$-dithiopyr were the highest in 0 and 0.25cm depth plots and decreased in over 0.5cm depth plots. However the extent in amount of distributed radioactivity in the plants among the different transplanting depths was narrow gradually with the growth of plants. Therefore, injury of transplanted rice by dithiopyr is little in over 0.5cm transplanting depth with burried basal stem and the inhibition on rice plants with extreme shallow transplanting such as 0 and 0.25cm depths should be due to more adsorption of dithiopyr.

• The Journal of Natural Sciences
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• v.4
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• pp.103-142
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• 1991

These studies were carried out to select somatic hybrid using selectable marker genes of Nicotiana glauca transformed by NPTII gene and Solanum tuberosum transformed by T- DNA, and to study characteristics of transformant. The results are summarized as follows. 1. Crown gall tumors and hairy roots were formed on potato tuber disc infected by A. tumefaciens Ach5 and A. rhizogenes ATCC15834. These tumors and roots could be grown on the phytohormone free media. 2. Callus formation from hairy root was prompted on the medium containing 2, 4 D 2mg/I with casein hydrolysate lg/l. 3. The survival ratio of crown gall tumor callus derived from potato increased on the medium containing the activated charcoal 0. 5-2. 0mg/I because of the preventions on the other hand, hairy roots were necrosis on the same medium. 4. Callus derived from hairy root were excellently grown for a short time by suspension culture on liquid medium containing 2, 4-D 2mg/I and casein hydrolysate lg/l. 5. The binary vector pGA643 was mobilized from E. coli MC1000 into wild type Agrobacteriurn tumefaciens Ach5, A. tumefaciens $A_4T$ and disarmed A. tuniefaciens LBA4404 using a triparental mating method with E. ccli HB1O1/pRK2013. Transconjugants were obtained on the minimal media containing tetracycline and kanamycin. pGA643 vectors were confirmed by electrophoresis on 0.7% agarose gel. 6. Kanamycin resistant calli were selected on the media supplemented with 2, 4-D 0.5mg/1 and kanamycin $100\mug$/ml after co- cultivating with tobacco stem explants and A. tumefaciens LBA4404/pGA643, and selected calli propagated on the same medium. 7. The multiple shoots were regenerated from kanamycin resistant calli on the MS medium containing BA 2mg/l. 8. Leaf segments of transformed shoot were able to grow vigorusly on the medium supplemented with high concentration of kanamycin $1000\mug$/ml. 9. Kanamycin resistant shoots were rooting and elongated on medium containing kanamycin $100\mug$/ml, but normal shoot were not. 10. For the production of protoplast from potato calli transformed by T-DNA and mesophyll tissue transformed by NPTII gene, the former was isolated in the enzyme mixture of 2.0% celluase Onozuka R-10, 1.0% dricelase, 1.0% macerozyme. and 0.5M mannitol, the latter was isolated in the enzyme mixture 1.0% Celluase Onozuka R-10, 0.3% macerozyme, and 0.7M mannitol. 11. The optimal concentrationn of mannitol in the enzyme mixture for high protoplast yield was 0.8M at both transformed tobacco mesophyll and potato callus. The viabilities of protoplast were shown above 90%, respectively. 12. Both tobacco mesophyll and potato callus protoplasts were fused by using PEG solution. Cell walls were regenerated on hormone free media supplemented with kanamycin after 5 days, and colonies were observed after 4 weeks culture.

• Applied Biological Chemistry
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• v.49 no.4
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• pp.328-333
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• 2006

Many plant extracts are known to have antioxidative effects. However, their activities can be reduced or disappeared during mass production process. The purpose of this study is to compare antioxidative effects of edible plant extracts distributed in Korea. forty three kinds of edible plant extracts commercially available in Korea were selected and investigated for their total phenolics contents and antioxidative potentials(DPPH radical and superoxide anion radical scavenging activities). In contents of total phenolics, the commercial plant extracts from Artemisia annua(whole plant), Ilex paraguariensis(leaf, Silybum marianum(fruit and leaf, Ulmus pumila(bark), Coliolus versicolor(fruit), and Curcuma longa(root and stem) contained over 70 mg/g of powder, DPPH radical scavenging activities($SC_{50}$, 50% scavenging concentration) of A. annua, I. paraguariensis, Pinus densiflora(leaf),S. marianum, U. pumila, and C. longa were $53.96{\pm}0.81\;ppm,\;24.61{\pm}2.12\;ppm,\;35.96{\pm}1.11\;ppm,\;57.46{\pm}2.13\;ppm,\;55.25{\pm}1.65\;ppm\;and\;12.99{\pm}1.67ppm$, respectively, while that of positive control(vitamin C) was $3.86{\pm}0.81\;ppm$. $SC_{50}$ values against superoxide anion radical of A. annua, Cinnamomum zeylanicum(bark), I. paraguariensis, Rubus coreanus(fruit and leaf), Morus alba(leaf), P. densiflora, S. marianum, U. pumila, C. versicolor, C. longa, Perilla frutescens var. acuta(leaf), and H. sabdariffa(leaf and newer) were $53.21{\pm}1.83ppm,\;50.12{\pm}2.12ppm,\;5.59{\pm}0.84ppm,\;41.60{\pm}8.93ppm,\;20.19{\pm}0.97ppm,\;15.19{\pm}1.66ppm,\;21.20{\pm}1.88ppm,\;15.71{\pm}0.91ppm,\;55.48{\pm}2.42ppm,\;52.12{\pm}2.44ppm,\;23.80{\pm}1.98ppm\;and\;11.14{\pm}0.51ppm$, respectively($SC_{50}$ value of vitamin C: $9.61{\pm}0.93ppm$). In particular, both 1 paraguariensis and P. densiflora had high content of phenolics as well as high scavenging activities of DPPH radical and superoxide anion radical. Consequently, above two commercial extracts may be useful as a source of antioxidative nutraceutics.

• Journal of the Korean Society of Food Science and Nutrition
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• v.38 no.11
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• pp.1571-1579
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• 2009

Antioxidant activities of ethanol and water extracts from Orostachys japonicus leaf, stem, and root were determined by rancimat method, DPPH radical scavenging effect, chelating effect, and reducing power analysis. The highest total phenolic compound (TPC) as 14.6 mg/g of dry sample and the strongest antioxidant activity in rancimat method (value of AI 1.98), DPPH radical scavenging effect (96% in 4 mg/mL), and reducing power (1.50 in 4 mg/mL) were observed in ethanol extracts from Orostachys japonicus leaf. Heat and pH stabilities on antioxidant activity of Orostachys japonicus leaf extract were studied through TPC and DPPH radical scavenging effect. As a result, the extracts from Orostachys japonicus leaf showed high stability. These results suggest that extracts from Orostachys japonicus leaf can be potentially used as proper natural antioxidant in the food industry.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.64 no.2
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• pp.144-151
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• 2019

The establishment rate of foxtail millet seeds is greatly affected by soil environment conditions. To enhance germination viability and stable production of foxtail millet seeds, it is important to select seeds with a high density. Therefore, this study tested the selection of high-quality seeds using salt solution (specific gravity: 1.000, 1.005, 1.010, 1.015, 1.020, 1.025, 1.030, 1.035, and $1.040g\;L^{-1}$) and investigated their germination rates, percentages of emergence, and seeding quality. In this study, three varieties were tested: 'Hwanggeum', 'Samdachal', and 'Kyeongkwan1'. The thousand seed weight of all three varieties increased proportionally with specific gravity. The highest thousand seed weight was observed at the selection with $1.040g\;L^{-1}$ specific gravity, which also had the highest germination rate of 88.3%, 86.7%, and 90.6%, for 'Hwanggeum'; 'Samdachal'; and 'Kyeongkwan1', respectively. The results of seeding quality indicated that higher the specific gravity during selection, the higher were the values of plant growth (plant height, leaf length, stem diameter, root length, root weight, and stem weight). All the three varieties were found to be longer or heavier for seeds selected at the specific gravity of $1.040g\;L^{-1}$. The packing germination viability investigation found that higher the specific gravity for selection of seeds, the higher was the percentage of emergence (PE) and the emergence rate index (ERI). The PE was the highest for seeds selected at $1.040g\;L^{-1}$ specific gravity (85.3, 83.0, and 87.0%), and ERI was also as high as $2.82d^{-1}\;m^{-1}$ or more at $1.040g\;L^{-1}$. Selection of seeds with salt solution resulted in high germination viability of foxtail millet. Therefore, sowing seeds selected at $1.040g\;L^{-1}$ specific gravity is expected to help substantially in increased productivity.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.34 no.1
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• pp.23-29
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• 1989

In order to investigate developmental habit and morphology of maize tillers, time and location of tiller development. number of tillers per plant, tiller angle, height and diameter of tillers and root systems of tillers were examined under field condition for maize with tillers. Materials used were mostly from Korean local lines and a few lines from other countries were also included for comparison. The time of the first tiller development was about 18 to 20 days after emergence when planted on May in Yusong. The second tiller appeared about 4 to 5 days after the first tiller appeared. The tiller number per plant varied with lines and hybrids and ranged from two to ten. The location of tiller development was usually basal nodes of the main stem. Each tiller appeared to have its own root system. The angle between tillers and main stem was variable depending upon the maizes and the tiller angle could be classified into three categories. The height of tillers was also variable and seemed to be under genetic control. The most productive tillers were found among the Korean local derivatives.

• Journal of Korean Society of Forest Science
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• v.54 no.1
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• pp.76-79
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• 1981

This thesis emphasize on diurnal changes of leaf water potential in relation to differences of cutting arrangement. 1. The more is leaves attached to the cutting, the higher is water stress in the cutting compared with less leaves. 2. The less is diameter of cut-part, the more are shown changes in increased and decrease in diurnal leaf water potential. 3. The more is length of stem, the more are shown water stress in diurnal leaf water potential 4. There is no differences between earthen-ball cutting and non-earthen ball cutting in diurnal leaf water potential. 5. Cuttings with two year's slip, compared with one year's leaf water potential increases slowly, dry weigh of root and numbers of rooted cutting are most likely dependent upon other factors rather than the water stress.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.57 no.4
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• pp.389-396
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• 2012

Melon (Cucumis melo) is an annual herbaceous plant of the family Cucurbitaceae. Phytophthora rot, caused by Phytophthora capsici is a serious threat to cucurbits crops production as it directly infects the host plant, and it is difficult to control because of variable pathogenicity. This study investigated the resistance of 450 accessions of melon germplasm against Phytophthora rot by inoculating the seedlings with sporangial suspension ($10^{5\;or\;6}$ zoosporangia/ml) of P. capsici. Disease incidence of Phytophthora rot was observed on the melon germplasm at 7-day intervals for 35 days after inoculation. Susceptible melon germplasm showed either severe symptoms of stem and root rot or death of the whole plant. Twenty out of 450 tested accessions showed less than 20% disease incidence, of which five accessions showed a high level of resistance against Phytopthtora rot. Five resistant accessions, namely IT119813, IT138016, IT174911, IT174927, and IT906998, scored 0% disease incidence under high inoculum density of P. capsici ($10^6$ zoosporangia/mL). We recommend that these candidate melon germplasm may be used as genetic resources in the breeding of melon varieties resistant to Phytophthora rot.