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Effects of $GeO_2$ and Citric Acid on Germanium Content of Callus and Plant in Angelica koreana MAX (강활(羌活)의 캘러스 및 식물체(植物體) 중(中) Ge함량(含量)에 미치는 $GeO_2$와 Citric Acid의 영향(影響))

  • Park, Byoung-Woo;Lee, Joong-Ho;Kwon, Tae-Oh
    • Korean Journal of Medicinal Crop Science
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    • v.4 no.2
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    • pp.101-108
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    • 1996
  • This study was conducted to investigate the effect of growth regulators and $GeO_2$ on the induction and proliferation of callus and the effect of $GeO_2$ and citric acid on the Ge content of callus from explants and plant, Angelica koreana Max. The results obtained were summarized as follows. The callus induction was most effective on MS (Murashige and Skoog) medium containing 1. 0ppm 2, 4 - D with petiolule. The proliferation of callus was most effective at 2. 0ppm 2, 4 - D on the medium, at 2. 5ppm $GeO_2$ on the medium containing 2. 0ppm 2, 4 - D, and at $0.\;1{\sim}1mM$citric acid on the medium at pH6 containing 2. 0ppm 2, 4 - D and 2. 5ppm $GeO_2.$ The more $GeO_2$ in MS medium up to 20ppm, the more Ge content in callus. Ge content in callus was highest when the medium was supplemented with 0. 1mM citric acid and the pH of medium was low. The Ge content in plant was high in order of leaf > root > stem. Application $GeO_2$ to the soil increased Ge content in plant and application of 1mM citric acid with $GeO_2$ resulted in increasing Ge content highest in plant, but application more than l0mM citric acid resulted in Ge content decreased. Application of $GeO_2$ increased Ge content in callus and plant but had a tendency to decrease some mineral content, on the other hand application of $0.\;1{\sim}1mM$ citric acid with $GeO_2$ had a tendency to increase mineral content.

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Effect of Tillage System and Livestock Manures on the Silage corn Production and NO3-N Concentration in Leaching Water (경운방법과 가축분뇨 시용이 옥수수의 생산성 및 질소의 용탈에 미치는 영향)

  • Jung, Min-Woong;Jo, Nam-Chul;Kim, Jong-Geun;Lim, Young-Chul;Choi, Ki-Choon;Yoon, Sei-Hyung;Lee, Ki-Won;Yook, Wan-Bang
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.29 no.3
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    • pp.211-216
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    • 2009
  • Approximately 43 million tons of livestock manure (LM) are produced each year on Korean farms. LM can be utilized as a valuable resource and/or it can contaminate water by runoff and leaching through the soil, when LM has been thoughtlessly applied to the land and directly discharged into the water. This experiment was carried out to investigate the effect of no-till system and LM application on dry matter (DM) yield of silage corn and $NO_3$-N concentration in leaching water of lysimeter installed in the experimental field. The treatments were replicated three times in split plot design. Main plots consisted of tillage systems, such as conventional tillage (CT) and no-tillage (NT). Sub plots consisted of the type of LM, such as chemical fertilizer (CF), composted cattle manure (CCM) and composted swine manure (CSM). The control plots were fertilized as commercial chemical fertilizer. DM yields of corn increased significantly in order to CF > CCM > CSM (p<0.05). DM yield of corn in CT increased as comparing with that of corn in NT. Plant height, ear height and stem diameter also increased in order to CF > CCM > CSM. In addition, the root weight in CT was increased as comparing with that of corn in NT. However, there was no interaction effects of between type of LM and tillage system. $NO_3$-N concentration in leaching water of LM application was less than 10 ppm, but $NO_3$-N concentration in CF exceeded 10 ppm which is safety level of drinking water during summer time (rainfall season).

Effect of Fertigation with Indigenous Microorganism and EM on Soil Chemical and Microbial Properties and Growth of Cherry Tomatoes (토착미생물과 EM 활용 액비 처리가 방울토마토의 토양 화학성과 미생물상 및 생장에 미치는 영향)

  • Choi, Hyun-Sug;Jung, Ji-Sik;Kuk, Yong-In;Choi, In-Young;Jung, Seok-Kyu
    • Journal of the Korea Organic Resources Recycling Association
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    • v.27 no.4
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    • pp.15-24
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    • 2019
  • The study was compared for soil chemical and microbial properties as well as growth of the cherry tomato (Lycopersicon esculentum var. cerasiforme) plants environmentally friendly gown for 3 years and 5 years, which had been fertigated with homemade liquid fertilizer (LF) with indigenous microorganism as an additional fertilizer. Treatment included LF with indigenous microorganism for 3 years (3-year IM-LF) and for 5 years (5-year IM-LF), with an effective microorganism for 10 years (EM-LF), which had been applied with 1,000 times of dilution in the farmhouse. IM-LF and EM-LF materials had increased pH pattern for 16 weeks, in particular for increase of 1.2 for EM-LF. IM-LF material contained slightly higher EC but similar level of 0.2 dS/m to EM-LF. For a pot experiment in the greenhouse, IM-LF treatment increased root dry weight of the cherry tomato plants. In the farmhouse experiment, IM-LF treatment increased to 7.5 of soil pH and 8.4 dS/m of EC, indicating high salt accumulation. EM-LF treatment increased to 62 g/kg of soil OM, which would have affected concentrations of macro essential nutrients, including T-N in the soil. However, the optimum soil chemical levels for growth of cherry tomato plants were observed on the IM-LF plots. EM-LF treatment increased number of bacteria and actinobacteria in the soil. EM-LF treatment increased concentrations of macro essential nutrients in the plants, except for P, with similar nutrient concentrations observed between 3-year IM-LF and 5-year IM-LF-treated plants. Leaf SPAD and PS II levels decreased in the plants treated with 3-year IM-LF. EM-LF treatment increased leaf width and length, number of leaves, canopy area, plant height, and stem diameter in the mid-term stage of growth, which were not significantly different between the treatments. EM-LF treated-plants had two times higher leaf dry weight than those of values observed on the IM-LF plants, which was the opposite result observed on the number of fruit.

Effect of Germanium Foliar Spray Application on Growth Characteristics and Germanium Absorption in Rice (게르마늄 엽면살포가 벼의 생육과 게르마늄 흡수에 미치는 영향)

  • Park, Jong-Hwan;Seo, Dong-Cheol;Kim, Seong-Heon;Lee, Choong-Heon;Lee, Seong-Tea;Choi, Jeong-Ho;Kim, Hong-Chul;Ha, Yeong-Rae;Cho, Ju-Sik;Heo, Jong-Soo
    • Korean Journal of Soil Science and Fertilizer
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    • v.45 no.4
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    • pp.649-656
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    • 2012
  • To obtain the basic information for agricultural utilization of germanium (Ge), the growth characteristics and Ge absorption in rice plant were investigated under different Ge concentrations by foliar spray application. The Ge concentrations were treated with 0 (control), 10, 20, 40 and $80mg\;L^{-1}$ in pot (1 5000$^{-1}$ a), respectively. The Ge absorption rate in rice by foliar spray application with $80mg\;L^{-1}$ in pot was higher in the order of leaf (5.75%) > stem (4.52%) > root (<0.01%). By foliar spray application, the Ge content in rice was higher in the order of $80mg\;L^{-1}$ > $40mg\;L^{-1}$ > $20mg\;L^{-1}$ > $10mg\;L^{-1}$. When rice was treated with $80mg\;L^{-1}$ of Ge, the Ge content in rice grain was higher in the order of rice bran ($0.21mg\;pot^{-1}$) $\gg$ brown rice ($0.04mg\;pot^{-1}$) ${\geq}$ polished rice ($0.03mg\;pot^{-1}$). By foliar spray application, the Ge uptake in rice bran was higher than that in other parts. Therefore, optimum Ge concentration by foliar spray application was $80mg\;L^{-1}$ in pot based on the results from the Ge treatments.

Carbon Storage and Uptake by Deciduous Tree Species for Urban Landscape (도시 낙엽성 조경수종의 탄소저장 및 흡수)

  • Jo, Hyun-Kil;Ahn, Tae-Won
    • Journal of the Korean Institute of Landscape Architecture
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    • v.40 no.5
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    • pp.160-168
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    • 2012
  • This study generated regression models to estimate the carbon storage and uptake from the urban deciduous landscape trees through a direct harvesting method, and established essential information to quantify carbon reduction from urban greenspace. Tree species for the study included Acer palmatum, Zelkova serrata, Prunus yedoensis, and Ginkgo biloba, which are usually planted as urban landscape trees. Tree individuals for each species were sampled reflecting various diameter sizes at a given interval. The study measured biomass for each part including the roots of sample trees to compute the total carbon storage per tree. Annual carbon uptake per tree was quantified by analyzing radial growth rates of stem samples at breast height. The study then derived a regression model easily applicable in estimating carbon storage and uptake per tree for the 4 species by using diameter at breast height(dbh) as an independent variable. All the regression models showed high fitness with $r^2$ values of 0.94~0.99. Carbon storage and uptake per tree and their differences between diameter classes increased as the diameter sizes got larger. The carbon storage and uptake tended to be greatest with Zelkova serrata in the same diameter sizes, followed by Prunus yedoensis and Ginkgo biloba in order. A Zelkova serrata tree with 15cm in dbh stored about 54kg of carbon and annually sequestered 7 kg, based on a regression model for the species. The study has broken new grounds to overcome limitations of the past studies which substituted, due to a difficulty in direct cutting and root digging of urban landscape trees, coefficients from the forest trees such as biomass expansion factors, ratios of below ground/above ground biomass, and diameter growth rates. Study results can be useful as a tool or skill to evaluate carbon reduction by landscape trees in urban greenspace projects of the government.

Changes in Growth Rate and Carbon Sequestration by Age of Landscape Trees (조경수목의 수령에 따른 생장율과 탄소흡수량 변화)

  • Jo, Hyun-Kil;Park, Hye-Mi
    • Journal of the Korean Institute of Landscape Architecture
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    • v.45 no.5
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    • pp.97-104
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    • 2017
  • Greenspace enlargement through proper landscape planting is essential to creating a low carbon society. This study analyzed changes in stem diameter growth rates(DGR), ratios of below ground/above ground biomass(B/A), and carbon sequestration by age of major landscape tree species. Landscape trees for study were 11 species and 112 individuals planted in middle region of Korea. The DGR and B/A were analyzed based on data measured through a direct harvesting method including root digging. The carbon sequestration by tree age was estimated applying the derived regression models. The annual DGR at breast height of trees over 30 years averaged 0.72 cm/yr for deciduous species and 0.83 cm/yr for evergreen species. The B/A of the trees over 30 years averaged 0.23 for evergreen species and 0.40 for deciduous species, about 1.7 times higher than evergreen species. The B/A by age in this study did not correspond to the existing result that it decreased as tree ages became older. Of the study tree species, cumulative carbon sequestration over 25 years was greatest with Zelkova serrata(198.3 kg), followed by Prunus yedoensis(121.7 kg), Pinus koraiensis(117.5 kg), and Pinus densiflora (77.4 kg) in that order. The cumulative carbon sequestration by Z. serrata offset about 5% of carbon emissions per capita from household electricity use for the same period. The growth rates and carbon sequestration for landscape trees were much greater than those for forest trees even for the same species. Based on these results, landscape planting and management strategies were explored to improve carbon sequestration, including tree species selection, planting density, and growth ground improvement. This study breaks new ground in discovering changes in growth and carbon sequestration by age of landscape trees and is expected to be useful in establishing urban greenspaces towards a low carbon society.

Ethnobotanical Study on the Traditional Knowledge of Vascular Plant Resources in South Korea (한국의 관속식물 전통지식에 대한 민속식물학적 연구)

  • Chung, Jae-Min;Kim, Hyun-Jun;Park, Gwang-Woo;Jeong, Hye-Ran;Choi, Kyung;Shin, Chang-Ho
    • Korean Journal of Plant Resources
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    • v.29 no.1
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    • pp.62-89
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    • 2016
  • The great part of the traditional knowledge on ethnobotanical plants and their uses is gradually vanishing due to industrialization, therefore ethnobotanical studies that explore and preserve the knowledge are in urgent needed before the knowledge are lost ever. This study was conducted to record and conserve the traditional knowledge of botanical taxa in Korea from 2006 to 2014. According to the survey results, derived from 17,328 sheets of 1,771 residents at 868 places, the ethnobotanical plants in Korea consisted of a total 924 taxa; 130 families, 493 genera, 813 species, 7 subspecies, 92 varieties and 12 form. Of them, herbs were 619 taxa (67.0%) and woody plants were 305 taxa (33.0%). 707 taxa (76.5%) out of 924 taxa grow wild, 44 taxa (4.8%) were cultivated wild species, 145 taxa (15.7%) were introduced for cultivation, and 28 taxa (3.0%) were naturalized plants. The analysis of usage for 924 taxa showed that the edible use was the highest with 58.9%, followed by medicinal with 26.9%, material with 3.7% and ornamental with 2.3%, respectively. The leaf of plant was the most useful part, followed by stem and root. Quantitative analysis of the ethnobotanical plants in Korea was performed by the basic values of FC, NU and UR, and indices of CI, RFC, RI and CV. As a result, The CI place Artemisia princeps in first position, followed by Aster scaber, Kalopanax septemlobus. The RFC place A. scaber in first position, followed by A. princeps, Aralia elata. The RI place A. princeps in first position, followed by Pinus densiflora, Morus alba. The CV place A. princeps in first position, followed by P. densiflora, K. septemlobus.

Effects of the Water Temperature Differences on Rice Growth in a Paddy Field (수온차이(水溫差異)가 수도생육(水稻生育)에 미치는 영향(影響))

  • Kim, Lee-Yul;Jo, In-Sang;Kim, Heung-Bae;Lee, Yong-Hwan;Cho, Byong-Ok
    • Korean Journal of Soil Science and Fertilizer
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    • v.18 no.4
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    • pp.359-365
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    • 1985
  • The four rice varieties, Kwanak, Nongbaek, Pungsan and Nampung-byo were cultivated to examine the growth conditions and grain yield in a Gyuam SiL paddy field irrigated with cold water around $17^{\circ}C$. Water temperatures for various distances from the inlet were measured. The results were summerized as follows. 1. Culm length, panicle exertion, diameter of the 3rd internode stem, heading date, fertilization rate, ripeness rate, no. of grains per panicle and grain yield were sensitive to water temperature. Panicle length, flag-leaf length, diameter of spike-neck and no. of panicles, however, were negligibly sensitive and there were no differences among varieties. 2. Elongations of the 2nd and 3rd internode steam were unsensitive to water temperature. 1st internode elongation and 4th internode development, however, were sensitive which was major factor in the culm length. 3. Ratios of partial dry weight to total dry weight were closely correlated with water temperature, Therefore, dry weight of grain was increased with water temperature while that of plant and root decreased. 4. Chlorophyll contents were decreased with the increment of water temperature and the highest at $20^{\circ}C$. 5. There was no grain yield at $17^{\circ}C$, Increases of grain yield to water temperature per unit were order at Pungsan > Kwanak > Nongbaek > Nampung. 6. The critical temperature in grain yield was $21^{\circ}C$. Optimum temperatures of Japonica ${\times}$ Indica types were higher than those of Japonica types.

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Uptake and Content of Ions on Several Organs of Mulberry Tree(Morus alba L.) in Relation to Different Nitrogen Sources in Water Culture (수경액중(水耕液中)의 공급질소형태(供給窒素形態)에 따른 상수(桑樹)의 이온 흡수와 기관별(器官別) 분포(分布)에 관한 연구)

  • Lee, Won-Chu;Ryu, Keun-Sup
    • Korean Journal of Soil Science and Fertilizer
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    • v.15 no.4
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    • pp.270-276
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    • 1982
  • Water culture experiment with mulberry (Morus alba L.)was carried out to investigate the ionic composition in the exudate and the ionic content in the organs (leaves, petioles, stem cortex, and roots) of mulberry tree with three different nitrogen sources ($NO_3-N$, $NH_4-N$, and $NH_4NO_3$). 1. Amount of exudate was much lower for $NH_4-N$ than for $NO_3-N$. 2. Total nitrogen content in the exudate was the highest in the NH4-N, the order of 2.2 times than in the $NO_3-N$. However, total nitrogen amount absorbed by tree per hour was proportional with the exudate amount. As the result, total nitrogen amount was higher 1.6 times in $NO_3-N$ ($289.6\;me\;plant^{-1}\;hr^{-1}$ than in $NH_4-N$. 3. Ammonium nitrogen depressed $Ca^{2+}$ uptake critically and even all of the anions, whereas $Mg^{2+}$, $K^+$ and $Na^+$ affected little. 4. Reduction of $NO_3$ may occured both in root and in leaves. 5. Content of cations and anions was highest in petioles, except $Ca^{2+}$ which was highest in leaves. As the result, petioles may be the storage organ of nutrients. 6. By increasing $NH_4-N$, ionic balance (C-A) decreased proportionally. 7. Nutrients amount in the exudate were approximatelly proportional with the amount in the roots. This suggested that roots may be the part diagnosed for nutrients. Being the sum of anions (${\Sigma}A$) higher than that of cations (${\Sigma}C$) in the roots, the ionic balance showed negative value.

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Effect of Ortet Age and Types of Cuttings on Rooting, Cyclophysis and Topophysis of Rooted Cuttings in Taxus cuspidata S. et Z (주목삽수(揷穗)의 모수령(母樹齡) 및 아조형태(芽條形態)가 발근(發根)과 묘형(苗型)에 미치는 영향(影響))

  • Goo, Gwan Hyo;Lee, Kang Young;Youn, Ki Sik;Kwon, Yeong Han
    • Journal of Korean Society of Forest Science
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    • v.79 no.4
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    • pp.359-366
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    • 1990
  • This study was carried out to investigate rooting rate, plagiotrophic growth and root-promoting effect of IBA in Taxus cuspidata S, et Z. cuttings. The results obtained were summarized as follows ; 1. As the ortet ages of cuttings were increased in 5, 10, to 20 years, the rooting rates were decreased in order of 85.7%, 81.7% and 62.4%, and the number of primary roots were also decreased in 5.3, 3.7 and 2.9, respectively. 2. Rooting rate by cutting types of lateral shoot were higher than those of main shoot type, significant at the 1% level. 3. IBA(l00ppm) treatment promoted the rooting rates and the number of primary roots. 4. As the ortet ages were increased in 5, 10 to 20 years, angles of central shoot of rooted cuttings which mesured cyclophysis were decreased in order of $75.9^{\circ}$, $68.5^{\circ}$ and $59.6^{\circ}$, respectively, significant at the 1% level. 5. Average angles showed $77.0^{\circ}$ for main shoot cutting, $65.9^{\circ}$ for upper lateral shoot and $61.7^{\circ}$ for lower lateral, which was significant at 1% level, and lateral shoot cutting showed more topophysis than main shoot cutting. 6. Shoot length of rooted cuttings from main shoot cutting was longer than that from lateral shoot significant at the 1% level. 7. Relationship between some characteristics showed highly positive correlation.

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