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Proteomic Analysis of Differentially Expressed Proteins in Bovine Endometrium with Endometritis

  • Choe, Chang-Yong;Park, Jeong-Won;Kim, Eun-Suk;Lee, Sung-Gyu;Park, Sun-Young;Lee, Jeong-Soon;Cho, Myung-Je;Kang, Kee-Ryeon;Han, Jae-Hee;Kang, Da-Won
    • The Korean Journal of Physiology and Pharmacology
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    • v.14 no.4
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    • pp.205-212
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    • 2010
  • Endometritis is one of the primary reasons for reproductive failure. In order to investigate endometritis-associated marker proteins, proteomic analysis was performed on bovine endometrium with endometritis. In bovine endometritis, desmin, $\alpha$-actin-2, heat-shock protein (HSP) 27, peroxiredoxin-6, luteinizing hormone receptor isoform 1, collectin-43 precursor, deoxyribonuclease-I (DNase-I), and MHC class I heavy chain (MHC-Ih) were up-regulated. In contrast, transferrin, interleukin-2 precursor, hemoglobin $\beta$ subunit, and potassium channel tetramerisation domaincontaining 11 (KCTD11) were down-regulated in comparison to normal endometrium. The proteomic results were validated by semiquantitative-PCR and immunoblot analysis. The mRNA levels of desmin, transferrin, $\alpha$-actin-2, HSP27, KCTD11, and MHC-Ih were up-regulated by over 1.5-fold, and showed a pattern similar to their proteomic profiles. Desmin and $\alpha$-actin-2 protein showed positive correlations between proteomic analysis and immunoblot analysis. These results suggest that desmin and $\alpha$-actin-2 may play important roles in endometritis-related function, and could be useful markers for the diagnosis of bovine endometritis.

Feasibility of Interference Alignment for Reverse Duplex in MIMO Cellular Networks with One-side Base Cooperation (다중 안테나를 사용하는 단방향 기지국 협력 역 듀플렉스 셀룰러 네트워크의 간섭 정렬 타당성)

  • Kim, Kiyeon;Jeon, Sang-Woon;Yang, Janghoon;Kim, Dong Ku
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.40 no.2
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    • pp.273-284
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    • 2015
  • The feasibility conditions of interference alignment (IA) are analyzed for reverse duplex systems, in which one cell operates as downlink (DL) while the other cell operates as uplink (UL) assuming one-sid base station (BS) cooperation. Under general multiple-input and multiple-output (MIMO) antenna configurations, a necessary condition and a sufficient condition for one-shot linear IA are established, i.e., linear IA without symbol or time extension. In several example networks, optimal sum degrees of freedom (DoF) is characterized by the derived necessary condition and sufficient condition. For some special class of networks, a sufficient condition is established in a more compact expression, which also yields the necessary and sufficient condition. Simulation results demonstrate that the proposed IA does not only achieve larger DoF but also significantly improves the sum rate in the practical signal-to-noise ratio (SNR) regime.

A Study on the Factors Changing the Number of Students according to the Urban Developments - Focusing on Incheon Metropolitan City - (도시개발(都市開發)에 따른 학교신설 요인(要因)에 관한 연구(硏究) - 인천광역시를 중심으로 -)

  • Lee, Hwa-Ryong;Dong, Jea-Uk;Cho, Chang-Hee
    • Journal of the Korean Institute of Educational Facilities
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    • v.16 no.6
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    • pp.3-12
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    • 2009
  • Reports on population movement(2000-2030) by the National Statistical Office show that the number of elementary school age population will be decreased by 1.4million from 2005 to 2020. It will effect both school size and the policies for school facilities, which have focused on downsizing class as the high standard of OECD nations. As the unfolding evidence is emerging that a number of schools and classes will be emptied out by 2030. This study aims at developing a objective and scientific device for estimate the number of students at future and evaluating the validity of school establishment. In doing so, it explores the relationship between the changing factors and the number of students. It proposes four factors such as the changes of population, the fertility rate, the number of apartment occupant and movement of population. As the result of the statistic analysis on the correlation coefficient, it finds out that the change of apartment occupant has a close correlation with the change in the number of student and has a deep effect on the establishment of school. Finally, it shows the construction of school according to urban developments during 1999-2008 in Incheon Metro-City. This study would help the authorities to expect the future number of student and to restrict overbuilding of surplus classes, finally the reasonable expenditure for school facilities.

A Studay on the Rainfall and Drought Days in Kyupgpook Area (경북지방(慶北地方)의 강수(降水) 및 무강수(無降水) 현상(現象) 조사(調査) 분석(分析))

  • Suh, Seung Duk;Jeon, Kuk Jin
    • Current Research on Agriculture and Life Sciences
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    • v.5
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    • pp.143-157
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    • 1987
  • In order to determine the design precipitation, the most probable daily precipitation and annual precipitation at every spot are calculated and iso - precipitation line are drawn. Probability of precipitation and drought phenomena of each gage station are analyzied by the method of frequency analysis from the statistical conceptions. The results summarized in this study are as the follows. 1. Annual mean precipitation in kyungpook area are 1044 mm, about 115 mm less than annual mean precipitation of Korea amounts to l1S9mm, and found to regionally unequal. 2. Monthly mean rainfall of July is 242.2mm, 23.2%, August 174.2mm, 16.7%, June 115mm, 11% and September 114.2mm, 10.9% and Rainfall depth of July-August are more than 40% of annual precipition. This shows notable summer rainy weather by typoon and low pressure storm and seasonal unbalance of water supply. 3. The relation among the maximum precipi.tation per day, per two continuous days and per three contnous days are caculated and the latter is found 31.0% increased rate of the first and the last 48.2% increased rate of first. 4. Probability precipitation in Kyungpook area are shown as 9.0%(5 year), 13.3%(10 year), 17.7%(20 year), 23.1%(50 year), 27.0%(100 year) and 31.1%(200 year) increased rate of each recurrence year compared with observed average annual precipitation. 5. From annual precipitation and maximum daily rainfall data probability of precipitation and precipitation isohyetal line are derived which shown as Table 11 and Fig. 8. 6. Drought days are divided 6 class and analysed results are shown on table 12. Average occurrence time of 10-14 continuous drought days are 2.3 time per year, 15-19 days are 0.9 time per year, 20-24 days are one per six years, 30-34 days are once per nine years and over than 35days are once per 25 years.

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Comparison of Endotracheal Intubation in Difficult Postures by Using the SALT(Supraglottic Airway Laryngopharyneal Tube)

  • Yun, Seong-Woo
    • Journal of the Korea Society of Computer and Information
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    • v.25 no.12
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    • pp.227-233
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    • 2020
  • The purpose of this study is to compare the speed, confidence, and ease of endotracheal intubation in a difficult posture by using the SALT(Supraglottic Airway Laryngopharyngeal Tube), and a direct laryngoscope to improve the ability to implement professional airway management. The subject of the study was an experimental study by a randomized crossover design, targeting 30 first-class emergency medical technicians working in J-do fire station, and the SPSS 20.0 version was used for data analysis. The endotracheal intubation by using SALT showed a significant difference in speed compared to endotracheal intubation by using a direct laryngoscope(p<.001), and also showed a significant difference in confidence and ease(p<.001). If it is difficult to endotracheal intubation by direct laryngoscope, or in the case of trauma patients, if SALT is used, safe and rapid intubation will be possible. In addition, to improve the efficiency of professional airway management, it is believed that the introduction of SALT in domestic sites is necessary.

Analysis of patient transfer status of private emergency ambulance services in Busan (부산 지역 민간 응급 이송업체의 환자 이송 현황 분석)

  • Han, Sung-Min;Park, Joung-Je;Lee, Jeong-Hyeok;Kook, Jong Won
    • The Korean Journal of Emergency Medical Services
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    • v.25 no.1
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    • pp.147-158
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    • 2021
  • Purpose: This study was conducted as a direct investigation of the data in the dispatch logbooks and status of patient transportation provided by private emergency transport companies in Busan. Methods: This study was conducted using SPSS 23.0 version for a total of 1,000 processed records of private emergency ambulance services in Busan from September 23, 2017 to November 5, 2019. Results: First, 100% of the emergency patient transfers by private emergency ambulances were carried out between medical institutions; 76.4% of all transfer patients had emergency conditions, and 86.0% had serious diseases. Second, 59.3% of the emergency patients were located at distances less than 10 km and 43.2%, at more than 10 km from the medical institutions. Third, 63.5% of the passengers were accompanied by first-class emergency rescuers according to the severity of the condition. Fourth, 92.7% of the reasons for the selection of medical institutions were transferred to places where professional care was available, accounting for most of the reasons for the selection. Finally, the medical institutions were selected according to the severity of the patient's condition; 76.5% patients were transported to institutions with a large number of doctors, and 42.9% of those were transported to specialized care institutions. Conclusion: This study collected data from 1,000 dispatch records of private emergency transport companies in Busan; these records reflect the government's policies to improve the emergency patient transfer system. The current status of emergency patient transfer offered by private transport companies was analyzed. All of the emergency patient transfers were carried out between medical institutions, and 76% of the transferred patients had emergency conditions.

Comparison of Endotracheal Intubation to Transfer - A Study of Simulation Using the SALT - (이송 중 기관내 삽관의 효율성 비교 - SALT를 이용한 시뮬레이션 연구 -)

  • Yun, Seong-Woo;Jung, Jun-Ho;Lee, Hyo-Ju;Choi, Mi-Young
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2022.10a
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    • pp.300-302
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    • 2022
  • The purpose of this study is to compare the speed, confidence, and ease of endotracheal intubation in a transfer posture by using the SALT(Supraglottic Airway Laryngopharyngeal Tube), and a direct laryngoscope to improve the ability to implement professional airway management. The subject of the study was an experimental study by a randomized crossover design, targeting 28 first-class emergency medical technicians working in J-do fire station, and the SPSS 20.0 version was used for data analysis. The endotracheal intubation by using SALT showed a significant difference in speed compared to endotracheal intubation by using a direct laryngoscope(p<.001), and also showed a significant difference in confidence and ease(p<.001). If it is transfer to endotracheal intubation by direct laryngoscope, or in the case of transfer patients, if SALT is used, safe and rapid intubation will be possible.

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Studies on the Interpretative Classification of Paddy Soils in Korea I : A Study on the Classification of Sandy Paddy Soils (우리나라 답토양(畓土壌)의 실용적분류(実用的分類)에 관(関)한 연구(硏究) -제1보(第一報) 사질답(砂質畓) 분류(分類)에 관(関)하여)

  • Jung, Yeun-Tae;Yang, Euy-Seog;Park, Rae-Kyung
    • Korean Journal of Soil Science and Fertilizer
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    • v.15 no.2
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    • pp.128-140
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    • 1982
  • The distribution and practical classification of sandy paddy soils, which have the most extensive acreage among low productive paddy soils in Korea and have distinctive improvement effects, were studied to propose a tentative new classification system of sandy textured paddy soils as a means of improving the "Paddy Soil Type Classification" scheme used. The results are summarized as follows; 1. The potential productivity of sandy textured paddy soils was about 86% of normal paddy and the coefficient of variation was relatively high indicating that the properties of soils included were not sufficiently homogeneous. 2. As the poorly drained and halomorphic (> 16 mmhos/cm of E.C. at $25^{\circ}C$) sandy soils are not included in the "Sandy Soil" type according to the criteria of "Soil Type Classification", the recommendation of "adding clay earth" become complicated, and the soil type have to change when the salts washed away or due to ground water table fluctuations. 3. Coarse textured soils were entirely included in the "Sandy Soils" in the tentative criteria of sandy soil classification proposed, and the sandy soils were subdivided into 4 subtypes that is "Oxidized leaching sandy paddy", Red-ox. intergrading sandy paddy", "Reduced accumulating sandy paddy" and "Reduced halomorphic sandy paddy". The system of sandy soil classification proposed were consisted of following categories; Type (Sandy paddy)-Sub-type (4)-Texture family (5)-Soil series (48). 4. The variation of productivities according to the proposed scheme was more homogenized than that of the present device. 5. The total extent of sandy paddy soils was 409, 902 ha (32.3% of total paddy) according to the present classification system, but the extent reached 492,983 ha (38.9%) by the proposed system. The provinces of Gyeong-gi (88.923ha), Jeon-bug (69.717 ha), Gyeong-bug (55.390 ha) have extensive acreage of sandy paddy soils, and the provinces that had high ratio of sandy paddy soils were Gang-weon (58.9%), Gyeong-gi (50.5%), Chung-bug (48.5%), Jeon-bug (41.0%) etc. The ratio was increased by the proposed scheme, e.g. 71.4% in the case of Gang-weon prov. 6. According to the suitability group of paddy soils, the sandy soils mostly belong to 3 class (69.1%) and 4 class (29.2%). Coarse loamy textural family (59.2%) and coarse silty (16.1 %) soils were dominantly distributed. 7. The "Red-ox. intergrading subtype" of sandy paddy pertinent to 49.6% (245,012 ha) while the "Oxidized leaching sub-type" reaches to 33.5% (64,890 ha) and the remained 16.9% (83,081ha) belong to "Reduced accumulating sub-type (14.0%) and "Reduced halomorphic sub-type (2.9%)" according to the proposed scheme.

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Water Uptake, Cotyledon Damage after Imbibition and Hypocotyl Elongation in Soybean with Different Seed Size and Color (콩 종실크기 및 종피색에 따른 침종후 수분흡수특성, 자엽손상 및 배축 신장력의 차이)

  • Park, Keum-Yong;Kim, Seok-Dong;Ryu, Yong-Hwan
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.39 no.4
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    • pp.331-338
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    • 1994
  • The experiment was conducted to determine if seed size and seed coat color of soybean might be effective in water uptake and cotyledon damage after imbibition, and hypocotyl elongation. Eight soybean cultivars were separated into two classes of large and small seed based on seed weight, and each class included two cultivars with yellow and black seed color, respectively. Small seed size group was superior in water uptake by seed for 24 hour in imbibition at $25^{\circ}C$ , but its differences decreased as soaking time increased. Small seed cultivars germinated faster and had better germination rate than large ones. However, cultivars with black seed coat showed more slow water uptake at initial time and faster germination than yellow seed, but in 24 hour after imbibition, cultivars with black seed coat had higher water uptake rate than yellow seeds. Small seed cultivar group showed no cotyledon damage in imbibition for 24 hour while large seed cultivars were damaged 78% of cotyledon, and black seed showed low cotyledon damage compared to yellow seed. Hypocotyl length was shorter in large seed rather than in small seed, but hypocotyl thickness in large seed was more thick than in small seed. In correlation coefficients, seed coat rate, embryo rate exhibited significantly negative association with seed weight, and the correlation of seed weight with water uptake in 3 hour after soaking was significantly negative, but in 24 hour showed positive correlation.

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Genetic Analysis of Ultrasound and Carcass Measurement Traits in a Regional Hanwoo Steer Population

  • Hwang, Jeong Mi;Cheong, Jae Kyoung;Kim, Sam Su;Jung, Bong Hwan;Koh, Myung Jae;Kim, Hyeong Cheol;Choy, Yun Ho
    • Asian-Australasian Journal of Animal Sciences
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    • v.27 no.4
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    • pp.457-463
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    • 2014
  • Ultrasound measurements of backfat thickness (UBF), longissimus muscle area (ULMA) and marbling score (UMS) and carcass measurements of carcass weight (CW), backfat thickness (BF), longissimus muscle area (LMA), and marbling score (MS) on 7,044 Hanwoo steers were analyzed to estimate genetic parameters. Data from Hanwoo steers that were raised, finished in Hoengseong-gun, Gangwon-do (province) and shipped to slaughter houses during the period from October 2010 to April 2013 were evaluated. Ultrasound measurements were taken at approximately three months before slaughter by an experienced operator using a B-mode real-time ultrasound device (HS-2000, FHK Co. Ltd., Tokyo, Japan) with a 3.5 MHz linear probe. Ultrasound scanning was on the left side between 13th rib and the first lumbar vertebrae. All slaughtering processes and carcass evaluations were performed in accordance with the guidelines of beef grading system of Korea. To estimate genetic parameters, multiple trait animal models were applied. Fixed effects included in the models were: the effects of farm, contemporary group effects (year-season at the time of ultrasound scanning in the models for UBF, ULMA, and UMS, and year-season at slaughter in the models for CW, BF, LMA, and MS), the effects of ultrasound technicians as class variables and the effects of the age in days at ultrasound scanning or at slaughtering as linear covariates, respectively for ultrasound and carcass measures. Heritability estimates obtained from our analyses were 0.37 for UBF, 0.13 for ULMA, 0.27 for UMS, 0.44 for CW, 0.33 for BF, 0.36 for LMA and 0.54 MS, respectively. Genetic correlations were strongly positive between corresponding traits of ultrasound and carcass measures. Genetic correlation coefficient between UBF and BF estimate was 0.938, between ULMA and LMA was 0.767 and between UMS and MS was 0.925. These results suggest that ultrasound measurement traits are genetically similar to carcass measurement traits.