• Title/Summary/Keyword: state coupling

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Efficient Production of ε-Poly-L-Lysine by Streptomyces ahygroscopicus Using One-Stage pH Control Fed-Batch Fermentation Coupled with Nutrient Feeding

  • Liu, Sheng-Rong;Wu, Qing-Ping;Zhang, Ju-Mei;Mo, Shu-Ping
    • Journal of Microbiology and Biotechnology
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    • v.25 no.3
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    • pp.358-365
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    • 2015
  • ε-Poly-L-lysine (ε-PL) is a homopolymer of L-lysine molecules connected between the epsilon amino and alpha carboxyl groups. This polymer is currently used as a natural preservative in food. Insufficient biomass is a major problem in ε-PL fermentation. Here, to improve cell growth and ε-PL productivity, various nitrogen-rich nutrients were supplemented into flask cultures after 16 h cultivation, marking the onset of ε-PL biosynthesis. Yeast extract, soybean powder, corn powder, and beef extract significantly improved cell growth. In terms of ε-PL productivity, yeast extract at 0.5% (w/v) gave the maximum yield (2.24 g/l), 115.4% higher than the control (1.04 g/l), followed by soybean powder (1.86 g/l) at 1% (w/v) and corn powder (1.72 g/l) at 1% (w/v). However, supplementation with beef extract inhibited ε-PL production. The optimal time for supplementation for all nutrients examined was at 16 h cultivation. The kinetics of yeast-extract-supplemented cultures showed enhanced cell growth and production duration. Thus, the most commonly used two-stage pH control fed-batch fermentation method was modified by omitting the pH 5.0-controlled period, and coupling the procedure with nutrient feeding in the pH 3.9-controlled phase. Using this process, by continuously feeding 0.5 g/h of yeast extract, soybean powder, or corn powder into cultures in a 30 L fermenter, the final ε-PL titer reached 28.2 g/l, 23.7 g/l, and 21.4 g/l, respectively, 91.8%, 61.2%, and 45.6% higher than that of the control (14.7 g/l). This describes a promising option for the mass production of ε-PL.

Observability Analysis of INS/GNSS System for Vehicles Moving with a Large Pitch Angle Change (피치각 변화가 큰 궤적에서의 INS/GNSS 통합항법 시스템 가관측성 분석)

  • Kim, Hyun-seok;Baek, Seung-jun;Kim, Hyung-Soo;Jo, Min-Su
    • Journal of Advanced Navigation Technology
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    • v.22 no.3
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    • pp.220-227
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    • 2018
  • The most widely used method for constructing an inertial navigation system (INS)/global navigation satellite system (GNSS) coupling system is to construct an integrated navigation system using a Kalman filter. However, depending on the trajectory, non-observable state variables may be generated. In this case, the state variables are not estimated. To solve this problem, a integrated navigation system is constructed and then an observability analysis is performed. In this paper, a 24th order position-matched Kalman filter is defined to design an INS/GNSS integrated navigation system for vehicles moving with a large pitch angle change. To verify the appropriateness of the error state variables applied to the Kalman filter, an observability analysis was performed. The trajectory was divided into five segments, and the piece-wise constant system (PWCS) was assumed for each segment, and the results were analytically analyzed. The analytical results and the simulation results confirm that the error state parameters of the Kalman filter are well-designed to the estimation side.

Application of an Augmented Predator-Prey Model to the Population Dynamics of Roe Deer in Jeju (제주도 노루의 개체수 관리를 위한 확장적 피식-포식모형의 적용에 관한 연구)

  • Jeon, Dae-Uk;Kim, Doa-Hoon
    • Korean System Dynamics Review
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    • v.12 no.2
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    • pp.95-126
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    • 2011
  • This paper aims at developing a System Dynamics model with an augmented predator-prey interaction structure to deal with the population management of roe deer in Jeju, Korea. Although people still regard the creature as one of the important tourist attractions, there has been much debate on the issues of the appropriateness of the population size of roe deers because they have been stigmatized as crop damagers, and roadkill/poaching victims due to their natural habit to move around from the top mountain to the lowland of the island. The model is therefore to incorporate these migrating and grazing behaviors into an augmented Lotka-Volterra model coupling roe deer population in both parts of the island to that of predators and preys of the species. The authors also provide a comprehensive set of dynamic hypotheses and relevant CLD/SFD to understand the population dynamics of roe deer and co-evolving species and perform the steady-state analysis of the proposed equation system to verify the model behavior of the numerical example lastly presented in this paper.

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Synthesis and Properties of Poly(1,1-diethynyl-1-silacyclopent-3-enes and -1-silacyclobutane)

  • Kim, Eun-Joo;Park, Jung-Woo;Kim, Yong-Rok;Park, Young-Tae
    • Bulletin of the Korean Chemical Society
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    • v.24 no.4
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    • pp.484-488
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    • 2003
  • Polycarbosilanes containing diacetylene and organosilacyclic groups, such as poly(1,1-diethynyl-1-silacyclopent-3-ene), poly(1,1-diethynyl-3-triethylsilyl-1-silacyclopent-3-ene), and poly(1,1-diethynyl-1- silacyclobutane), were synthesized by the Glaser oxidative coupling polymerization reactions of 1,1-diethynyl-1-silacyclopent-3-ene, 1,1-diethynyl-3-triethylsilyl-1-silacyclopent-3-ene, and 1,1-diethynyl-1-silacyclobutane, respectively. These materials are almost insoluble in usual organic solvents such as $CHCl_3$ and THF. The polymers were characterized by using several spectroscopic methods in solid state. FTIR spectra of all the polymeric materials show that the characteristic C≡C stretching frequencies appear at 2146-2170 $cm^{-1}$, in particular. The polymers in the solid state show that the strong maximum excitation peaks appear at 255-257 nm and the strong maximum fluorescence emission bands at 401-402 nm. About 71-87% of the initial polymer weights remain at 400 ℃ in nitrogen according to thermogravimetric analysis.

Nonlinear wind-induced instability of orthotropic plane membrane structures

  • Liu, Changjiang;Ji, Feng;Zheng, Zhoulian;Wu, Yuyou;Guo, Jianjun
    • Wind and Structures
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    • v.25 no.5
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    • pp.415-432
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    • 2017
  • The nonlinear aerodynamic instability of a tensioned plane orthotropic membrane structure is theoretically investigated in this paper. The interaction governing equation of wind-structure coupling is established by the Von $K\acute{a}rm\acute{a}n's$ large amplitude theory and the D'Alembert's principle. The aerodynamic force is determined by the potential flow theory of fluid mechanics and the thin airfoil theory of aerodynamics. Then the interaction governing equation is transformed into a second order nonlinear differential equation with constant coefficients by the Bubnov-Galerkin method. The critical wind velocity is obtained by judging the stability of the second order nonlinear differential equation. From the analysis of examples, we can conclude that it's of great significance to consider the orthotropy and geometrical nonlinearity to prevent the aerodynamic instability of plane membrane structures; we should comprehensively consider the effects of various factors on the design of plane membrane structures; and the formula of critical wind velocity obtained in this paper provides a more accurate theoretical solution for the aerodynamic stability of the plane membrane structures than the previous studies.

Vibrational Structure and Predissociation of Ar-CO2 by CO2 Symmetric Stretching Mode Coupled with Ar Motion

  • Jung, Jae-Hoon;Sun, Ho-Sung
    • Bulletin of the Korean Chemical Society
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    • v.23 no.2
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    • pp.245-252
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    • 2002
  • The computationally simple quantum mechanical method (VSCF-DWB-IOS) has been applied to studying the Ar-$CO_2$ vibrational predissociation phenomenon. The new methodology utilizes the vibrational self-consistent field method to determine the vibrational structure of the van der Waals complex, the distorted-wave Born approximation for dissociating process, and the infinite-order sudden approximation for the continuum dissociating product of $CO_2$. The dissociation due to the coupling of the symmetric stretching vibrational motion of $CO_2$ with the motion of the Ar van der Waals mode has been extensively investigated. The lifetimes of transient excited vibrational states, linewidths of absorption peak, and the rotational state distributions of the product, $CO_2$ have been computed. It has been found that the lifetime of the Ar-$CO_2$ in excited vibrational state is very long compared with that of triatomic van der Waals complexes and the product $CO_2$ carries a major portion of dissociation energy as a rotational energy.

Spray Combustion Analysis for Unsteady State in Combustion Chamber of Liquid Rocket Engine Considering Droplet Fluctuation (액적변동을 고려한 액체로켓의 연소실 내 비정상 분무연소 해석)

  • Jeong, Dae-Kwon;Roh, Tae-Seong
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • 2006.11a
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    • pp.175-178
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    • 2006
  • A numerical study for spray combustion of fluctuated fuel and oxidizer droplets injected into combustion chamber has been conducted for the analysis of spray combustion considering characteristics of injector. The 2 dimensional unsteady state flow fields have been calculated by using QUICK Scheme and SIMPLER Algorithm. As the spray model, DSF model and Euler-Lagrange Scheme have been used. The sine Auction has been used for droplet fluctuation model of fuel and oxidizer, while the coupling effects of the droplets between gas phase and evaporated vapor have been calculated by using PSIC model.

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Improvement of Colored Thread Algorithm for Loop Prevention (루프 방지를 위한 칼라 스레드 알고리즘의 개선)

  • 김한경
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.26 no.12C
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    • pp.167-173
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    • 2001
  • Colored thread algorithm suggested by Ohba is understood to be effective to prevent from establishing path in loop. 3 kinds of thread state, such as null, colored, transparent, defined in colored thread algorithm which makes difficult and complex to design actions of the thread events, is reconfigured and improved to a 4 states to make the finite state machines simple. The original colored thread algorithm fonds loops by receiving thread of a same color that was generated by the initiator itself and confirms the loops by hop count of unknown thread traversed again along the looped path. This method is modified as to use the same color instead of generating new colored thread for the looped path, leading to save the traversing time. And also redefined the events to make easy coupling with existing protocols.

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Steady- and Transient-State Analyses of Fully Ceramic Microencapsulated Fuel with Randomly Dispersed Tristructural Isotropic Particles via Two-Temperature Homogenized Model-II: Applications by Coupling with COREDAX

  • Lee, Yoonhee;Cho, Bumhee;Cho, Nam Zin
    • Nuclear Engineering and Technology
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    • v.48 no.3
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    • pp.660-672
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    • 2016
  • In Part I of this paper, the two-temperature homogenized model for the fully ceramic microencapsulated fuel, in which tristructural isotropic particles are randomly dispersed in a fine lattice stochastic structure, was discussed. In this model, the fuel-kernel and silicon carbide matrix temperatures are distinguished. Moreover, the obtained temperature profiles are more realistic than those obtained using other models. Using the temperature-dependent thermal conductivities of uranium nitride and the silicon carbide matrix, temperature-dependent homogenized parameters were obtained. In Part II of the paper, coupled with the COREDAX code, a reactor core loaded by fully ceramic microencapsulated fuel in which tristructural isotropic particles are randomly dispersed in the fine lattice stochastic structure is analyzed via a two-temperature homogenized model at steady and transient states. The results are compared with those from harmonic- and volumetric-average thermal conductivity models; i.e., we compare $k_{eff}$ eigenvalues, power distributions, and temperature profiles in the hottest single channel at a steady state. At transient states, we compare total power, average energy deposition, and maximum temperatures in the hottest single channel obtained by the different thermal analysis models. The different thermal analysis models and the availability of fuel-kernel temperatures in the two-temperature homogenized model for Doppler temperature feedback lead to significant differences.

Ultraviolet Photoelectron spectroscopy Study of Colossal Magnetoresistive $La_{0.7-x}P_rxCa_{0.3}MnO_3$

  • Lee, Chang-Won;Hoon Koh;Noh, Han-Jin;Park, Jong-Hyurk;Kim, Hyung-Do;Moonsup Han;Oh, Se-Jung;Eom, dai-Jin;Noh, Tae-Won
    • Proceedings of the Korean Vacuum Society Conference
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    • 1999.07a
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    • pp.172-172
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    • 1999
  • Perovskite Manganese Oxide has been intensively investigated since the discovery of the colossal magnetoresistive(CMR) effect. In this paper, we studied the effect of temperature dependence and various doping dependence of rare earth site ions of La0.7-xPrxCa0.3MnO3 series using Ultraviolet Photoelectron spectroscopy(UPS). They show unusual temperature dependent features and the doped rare earth ions seem to affect the electron-phonon coupling strongly. We found clear evidence of metal-insulator transition from the spectral density at the Fermi level. but the transition temperature is lower than that deduced from transport measurements. Also we found that the spectral features change as time goes on implying that the surface of these materials is somewhat unstable in the vacuum. We can conclude from these results that the surface oxygen atoms correlated to the hopping electrons can escape from the material into the vacuum and that the surface state of these material is different from the bulk state.

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