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Identification of Genes Modulated by High Extracellular Calcium in Coculture of Mouse Osteoblasts and Bone Marrow Cells by Oligo Chip Assay

  • Kim, Hyung-Keun;Song, Mi-Na;Jun, Ji-Hae;Woo, Kyung-Mi;Kim, Gwan-Shik;Baek, Jeong-Hwa
    • International Journal of Oral Biology
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    • v.31 no.2
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    • pp.53-65
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    • 2006
  • Calcium concentration in the bone resorption lacunae is high and is in the mM concentration range. Both osteoblast and osteoclast have calcium sensing receptor in the cell surface, suggesting the regulatory role of high extracellular calcium in bone metabolism. In vitro, high extracellular calcium stimulated osteoclastogenesis in coculture of mouse osteoblasts and bone marrow cells. Therefore we examined the genes that were commonly regulated by both high extracellular calcium and $1,25(OH)_2vitaminD_3(VD3)$ by using mouse oligo 11 K gene chip. In the presence of 10 mM $[Ca^{2+}]e$ or 10 nM VD3, mouse calvarial osteoblasts and bone marrow cells were co-cultured for 4 days when tartrate resistant acid phosphatase-positive multinucleated cells start to appear. Of 11,000 genes examined, the genes commonly regulated both by high extracellular calcium and by VD3 were as follows; 1) the expression of genes which were osteoclast differentiation markers or were associated with osteoclastogenesis were up-regulated both by high extracellular calcium and by VD3; trap, mmp9, car2, ctsk, ckb, atp6b2, tm7sf4, rab7, 2) several chemokine and chemokine receptor genes such as sdf1, scya2, scyb5, scya6, scya8, scya9, and ccr1 were up-regulated both by high extracellular calcium and by VD3, 3) the genes such as mmp1b, mmp3 and c3 which possibly stimulate bone resorption by osteoclast, were commonly up-regulated, 4) the gene such as c1q and msr2 which were related with macrophage function, were commonly down-regulated, 5) the genes which possibly stimulate osteoblast differentiation and/or mineralization of extracellular matrix, were commonly down-regulated; slc8a1, admr, plod2, lox, fosb, 6) the genes which possibly suppress osteoblast differentiation and/or mineralization of extracellular matrix, were commonly up-regulated; s100a4, npr3, mme, 7) the genes such as calponin 1 and tgfbi which possibly suppress osteoblast differentiation and/or mineralization of extracellular matrix, were up-regulated by high extracellular calcium but were down-regulated by VD3. These results suggest that in coculture condition, both high extracellular calcium and VD3 commonly induce osteoclastogenesis but suppress osteoblast differentiation/mineralization by regulating the expression of related genes.

A Study on The Construction of Choryang-Waegwan (초량왜관의 조영활동에 관한 연구)

  • Chung Ye-Jung
    • Proceedings of the Korean Institute of Navigation and Port Research Conference
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    • 2005.10a
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    • pp.195-216
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    • 2005
  • This study concerns especially Choryang-Weagwan which was the largest Japanese House left in Pusan through Chosun Dynasty.Choryang-Waegwan was known to have been jointly constructed by Korean and Japanese carpenters. Therefore, Weagwan was a place for exchange of architectural tradition (special features such as sliding door and straw mat) between Korean and Japan. Judging from this point of view, It is certain that mutual influences helped to shape architecture of Choryang-Weagwan. After establishment Choryang-Weagwan was gradually extended, owing to the prosperity of trade with Japan. But since late 18th century government of Chosun did not give as much care to maintaining Choryang-Weagwan as a result of deteriorating condition of commercial and diplomatic relations with Japan. From the beginning of Choryang-Weagwan construction, Superintendents of the construction were called Hun-do and Byl-cha, who acted as official interpreters as well. And, during construction works, they were called Gamdong-gwan. At the start of construction, Weagwan was built partly in Japanese-style by the carpenters from Tokugawa Shogunate. But as time passed, the participation rate of Japanese carpenters diminished gradually. After 1831, Japanese technician vanished extremely and repairing construction was continued by the Korean

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An Experimental Study on the Fundamental Characteristics of LPG Gas Injections System (LPG 가스분사시스템의 기초특성에 대한 실험적 연구)

  • Jang, Yeol-Sung;Woo, Sung-Dong;Kim, Hyeong-Sig;Park, Chan-Jun;Ohm, In-Yong
    • Journal of Energy Engineering
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    • v.15 no.4 s.48
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    • pp.277-283
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    • 2006
  • In this study, butane 100% was used as fuel to verify the real fuel effect such as vapor pressure variation due to temperature change. A MPI fuel injection system for V-6 engine, which has reverse 'L' type cross section to minimize the possibility of liquid phase injection, was composed and one bank was operated under sequential injection scheme. Flow rate were measured according to injection duration, interval, and pressure. Also occurring of liquid phase injection was monitored with varying vaporizer and fuel rail temperature. The result shows that basic characteristics of injection is a relatively difference between air and LPG injection. Under cold start condition, however, the occurrence of liquid injection becomes more severe as the pressure increases, and sufficiently high temperature both in vaporizer and fuel rail is very important to insure gaseous injection. In addition, the temperature of vaporizer plays more important role in keeping LPG vapor state and the reverse 'L' type cross section of the rail is available to prevent liquid injection.

DNA Watermarking Method based on Random Codon Circular Code (랜덤 코돈 원형 부호 기반의 DNA 워터마킹)

  • Lee, Suk-Hwan;Kwon, Seong-Geun;Kwon, Ki-Ryong
    • Journal of Korea Multimedia Society
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    • v.16 no.3
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    • pp.318-329
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    • 2013
  • This paper proposes a DNA watermarking method for the privacy protection and the prevention of illegal copy. The proposed method allocates codons to random circular angles by using random mapping table and selects triplet codons for embedding target with the help of the Lipschitz regularity value of local modulus maxima of codon circular angles. Then the watermark is embedded into circular angles of triplet codons without changing the codes of amino acids in a DNA. The length and location of target triplet codons depend on the random mapping table for 64 codons that includes start and stop codons. This table is used as the watermark key and can be applied on any codon sequence regardless of the length of sequence. If this table is unknown, it is very difficult to detect the length and location of them for extracting the watermark. We evaluated our method and DNA-crypt watermarking of Heider method on the condition of similar capacity. From evaluation results, we verified that our method has lower base changing rate than DNA-crypt and has lower bit error rate on point mutation and insertions/deletions than DNA-crypt. Furthermore, we verified that the entropy of random mapping table and the locaton of triplet codons is high, meaning that the watermark security has high level.

Monitoring Vegetation Phenology Using MODIS in Northern Plateau Region, North Korea (MODIS자료를 이용한 북한 개마고원 및 백무고원 식생의 생물계절 모니터링)

  • Cha, Su-Young;Seo, Dong-Jo;Park, Chong-Hwa
    • Korean Journal of Remote Sensing
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    • v.25 no.5
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    • pp.399-409
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    • 2009
  • Many researches have shown that NDVI provides a potential methods to derive meaningful metrics that describe ecosystem functions. In this paper we investigated the use of the MODIS NDVI (Normalized Difference Vegetation Index) to monitor vegetation phenology dynamics of Northern plateau region, North Korea, during last 9-years (2000~2008). The findings of this paper can be summarized as follows. First, the length of growing season ranged from a low of 128 days in 2003 to a high of 176 days in 2000 and 2005. On the average of the last 9 years, the highest NDVI of 0.86 was marked on 28 July. Greenup onset occurs at the start of May, while the senescence begins between late September and October. Second, these annual vegetation cycles were compared with Seorak and Jiri Mountain regions of South Korea which have similar vegetation condition. Greenup onsets in South Korea were observed earlier than those of North Korea and the average time lag between the South and North Korea in Greenup was about 16 days which is a time-resolution of remotely sensed data. Sub-alpine conifers of such areas may be severely affected by the large of phenological characteristics due to the global warming trend.

A New Early Flowering, Spray Chrysanthemum Cultivar for Cut Flower, "Pinky" with Single Type and Pink Petals (조기개화성의 절화용 핑크색 흩꽃 스프레이국화 "핑키" 육성)

  • Hwang, Ju-Chean;Chin, Young-Don;Chung, Yong-Mo;Kim, Su-Gyeong
    • Korean Journal of Breeding Science
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    • v.40 no.2
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    • pp.196-200
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    • 2008
  • A new spary chrysanthemum (Dendranthema grandiflorum) cultivar 'Pinky' was bred from a cross between 'Rima' and 'Yen'. Progenies were investigated from 2004 to 2006 under shading culture in spring and retarding culture in autumn and the promising line was selected based on the performance of year-round production, white rust resistance and vase life. Pinky flowered around 20 October, and year-round production was possible by shading and lighting. Its flower was 3.1 cm in diameter, and had 38.4 petals and 26.8 flowers per stem in autumn. Its petal was narrow (0.4 cm) and pink with green in color at the flower center. Days to flower from short-day start for 'Pinky' was about 47 in spring. 'Pinky' showed a vase life of 20.9 days in autumn. This cultivar was commercially registered in 2007. 'Pinky' would be suitable for culture under the greenhouse and rain shelter condition in Korea.

A New Spray Chrysanthemum Cultivar, "Water Fog" with Single Type and White Petals for Cut Flower (절화용 백색 흩꽃 스프레이국화 "워트포그" 육성)

  • Hwang, Ju-Chean;Chin, Young-Don;Chung, Yong-Mo;Kim, Su-Gyeong
    • Korean Journal of Breeding Science
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    • v.40 no.2
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    • pp.159-163
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    • 2008
  • A New spary chrysanthemum (Dendranthema grandiflorum) cultivar 'Water Fog' was bred by open pollination of 'Angaesoguk'. Its characteristics were investigated three times from 2004 to 2006 under forcing culture in spring and retarding culture in autumn. The natural flowering time of 'Water Fog' was 24 October, but year-round production was possible by shading and lighting. It had flower diameter of 2.3 cm, 46.5 petals and 34.4 flowers per stem in autumn. The petal was narrow(0.4cm) and the color of petal was white with presence of green at flower center. Days to flower from short-day start for 'Water Fog' was about 48 in spring. 'Water Fog' showed the vase life of 22.6 days in autumn. This cultivar was registered for a commercialization in 2007. 'Water Fog' would be cultured under greenhouse and rain shelter condition in Korea.

The Ways to Develop the Arbitration Industry in Korea (한국 중재산업 발전 방안)

  • Yoon, Jin-Ki
    • Journal of Arbitration Studies
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    • v.28 no.4
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    • pp.3-42
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    • 2018
  • This paper aims to explore ways to develop the arbitration industry in Korea. The prospects for the promotion of the arbitration industry in Korea are never dim. International arbitration competitiveness is somewhat lower than its competitors at present, but the international economic base to support it is solid, and the domestic arbitration environment seems to be sufficient to support the development possibility of arbitration. Since geographical and economic factors have already been defined, Korea must at least improve the arbitration act with passion and vision for the best one. The arbitration act that is the most accessible to arbitration consumers is the best arbitration act. The important thing is to have an arbitration act that makes people want to use more than litigation or other dispute resolution procedures. There is no hope of remaining as a "second mover" in the field of arbitration law. One should have a will and ambition to become a "first mover" even if it is risky. Considering the situation of the current arbitration law, it is necessary to start an arbitration appeal system in order to become a consumer-friendly arbitration law, and it is necessary to examine ways of integrating the grant of execution clause and enforcement application procedures. The abolition of the condition of Article 35 of the Arbitration Act, which rules the validity of the arbitration award, will help promote international arbitration. Exclusion agreements of setting aside against arbitration awards must also be fully recognized. It is also important to publish a widely cited international arbitration journal. In order to respond to the fourth industrial revolution era, it is necessary to support the establishment of a dispute resolution system that utilizes IT technology. In order to actively engage the arbitrators in the market, it is necessary to abolish the regulations that exist in the Attorneys-at-Law Act. There is also a need to allocate more budget to educate arbitration consumers and to establish arbitration training centers to strengthen domestic arbitration education. It is also necessary to evaluate and verify the Arbitration Promotion Act so that it can achieve results. In the international arbitration market, competition is fierce and competitors are already taking the initiative, so in order not to miss the timing, Korea needs to activate international arbitration first. In order to activate international arbitration, the arbitration body needs to be managed with the same mobility and strategy as the agency in the marketplace. In Korea, unlike in Singapore and Hong Kong, it is necessary to recognize that the size of the domestic arbitration market is very likely to increase sharply due to the economic size of the country and the large market potential it can bring from litigation. In order to promote the arbitration industry, what is most important is to make arbitration activities in accordance with the principles of the market and to establish an institutional basis to enable competition. It is urgently required to change the perception of the relevant government departments and arbitration officials.

Development Status of Technology Demonstration Model for Staged Combustion Cycle Engine (다단연소사이클 엔진 기술검증시제 개발 현황)

  • Kim, Chaehyoung;Lee, Jungho;Woo, Seongphil;So, Younseok;Yi, SeungJae;Lee, Kwang-Jin;Cho, Namkyung;Han, Yeoungmin;Kim, Jin-han
    • Journal of the Korean Society of Propulsion Engineers
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    • v.23 no.4
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    • pp.104-111
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    • 2019
  • Staged combustion cycle engines exhibit higher combustion performance compared with open cycle engines with a gas generator. An advanced research of the staged combustion cycle engine is going on for the next program following the KSLV-II program. Various experiments have been carried out for the technology demonstration model, TDM0A and TDM0B. The experiments on the combustion performance are aimed to understand the engine start condition and combustion characteristics. They also aim to develop the oxidizer-rich pre-burner and the combustor of the staged combustion cycle engine. The engine-shaped model, TDM1A is fabricated based on the experimental data. The combustion experiment of the TDM1A shows that the combustion pressure of the combustor is approximately 91 bar and the turbine rotation is approximately 28,00 rpm. The result is stable and satisfies the development requirements. The present paper reports on the development process and characteristics of engine models from TDM0A to TDM1A.

Analysis of dental hygiene assessment data of recall patients (mainly 20s age)

  • Choi, Hye-Jung;Woo, Hee-Sun
    • Journal of the Korea Society of Computer and Information
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    • v.27 no.6
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    • pp.131-137
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    • 2022
  • As the age increases, the oral cavity, that is, the teeth and periodontium, also begin to age, and accordingly, a preparation process is required. The preparation process is an important period for oral health management to start continuously with oral health education consisting of knowledge, attitude, and behavior from the 20s. Therefore, to design a clinical dental hygiene course for patients who visited a dental clinic in Gyeonggi-do and received continuous care in an oral health care room after treatment, we tried to analyze the data of the dental hygiene assessment. As a dental hygiene assessment tool, based on personal information and general medical history, dental visit experience, bleeding on probing(BOP), bad breath measurement, phase contrast microscopy, and O'Leary index were performed. The number of subjects who had dental visits was 75.4% and those without experience were 24.6%, and as a result of the periodontal examination, generally bleeding was found in 76.3%. In preventive oral care, the stage of dental hygiene assessment in the 20s is an important first step. From this point on, it is an important time to be systematically habituated so that you can take responsibility for your own oral condition. Therefore, in this study, the results of dental hygiene assessment through oral examinations of subjects in their 20s are derived and presented as basic data for the development of dental hygiene performance competency of dental hygienists during the clinical dental hygiene process in oral health education and oral health management.