• Development and Reproduction
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• v.12 no.3
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• pp.283-288
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• 2008

The Pseudogobio esocinus were caught at Wyuleo-ri, Gyeombaek-myeon, Boseong-gun, Jeollanamdo from April to May 2003. The fishes were incubated in transparent aquarium located at the laboratory of Chonnam National University, and their embryonic and larval development were observed. The fertilized eggs were spherical, semitransparent, and adhesive, and were $1.98{\pm}0.19mm$ (n=50) in diameter. The embryo, including 31$\sim$32 myotomes, hatched through egg membrane at 164 hrs after fertilization. The newly-hatched larvae were $4.61{\pm}0.83mm$ (n=10) in total length (TL). At that moment, yolk was not absorbed, and mouth and anus were not open. Star and spot shaped melanophores were distributed on the lens, and dorsal, ventral, and caudal parts. At 42 days after hatching, larva was $16.22{\pm}0.65mm$ (n=10) in TL. Melanophores were scaterred at head, back, and side parts. Morphological features of the embryo were transferred to juvenile stage showing similar features with those of the adult fish.

• Development and Reproduction
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• v.13 no.4
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• pp.281-289
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• 2009

The slime flounder Microstomus achne were caught at Geomun Island, Yosu-si, Jeollanamdo from January to March in 2006. The fertilized eggs were observed for morphological development of egg, embryo and larva. Eggs were colorless transparent, separative pelagic, absent of oil globule, and the diameter was 1.64${\pm}$0.03 mm (n=50). The eggs were hatched at 168 hours 40 minutes after fertilization in the range of $9.8\sim13.0^{\circ}C$ (mean $11.4{\pm}1.6^{\circ}C$). Total length of newly hatched larva was 4.05${\pm}$0.18 mm (n=20). The larva had developed membranous fin showing waterdrop-shaped structure, and their mouth and anus were not open. The myotomes were 14~15+33~34=47~49. The egg yolks were 1.64${\pm}$0.12 mm in major axis, and 1.23${\pm}$0.19 mm in minor axis. At 12 days after hatching, the total length was 7.32${\pm}$0.42 mm(n=20). The egg yolk was completely absorbed and transferred to post larval stage. Star-shaped melanophores and branch-shaped xanthophores in the edge of membranous fin were more densed. Chrysanthemum-shaped melanophores in the notochord were densed and formed 4~5 melanophore bands. At 90~93 days after hatching, morphological features of the larva, 19.91${\pm}$1.63 mm TL(n=20), were transferred to juvenile stage showing similar features with those of the adult fish.

• Journal of Animal Environmental Science
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• v.13 no.1
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• pp.45-52
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• 2007

To evaluate the feed value of starfish, antimicrobial effects of its extract, nutrients contents, concentration of amino acids and its bioavailability were tested. Steaming and ether processes were applied to obtain the extract from starfish for antimicrobial effects examination. The starfish was dried at $60^{\circ}C$ for 3 days before grinding for processing and fermentation. Ground starfish(GS), extruded starfish(ES), fermented starfish(EFS) were added with enzyme and without enzyme(Non enzyme fermented starfish : NEFS). Then the nutrient composition and bioavailability of those were analyzed. The extract from starfish showed no inhibition of the growth of lactobacillus and pathogenic bacteria. Protein content showed significantly higher 62.86% and 52.82%, respectively in EFS and NEFS than GS and EGS(p<0.05). The Ca content of GS, EGS, EFS and NEFS was 17.26%, 18.26%, 5.37% and 8.55%, respectively. It was low in EFS and NEFS due to measure the Ca content after fermentation. Total amino acid was 17.17 mg/g, 20.28 mg/g, 36.30 mg/g and 29.96 mg/g in GS, EGS, EFS and NEFS, respectively. The ratio of total amino acid to protein tended to show the similar tendency as total amino acid. Both total amino acid and its ratio to protein were increased by the fermentation. Bioavailability of the protein and Ca showed more 80% in EFS and NEFS. The nutrients availability of EFS were significantly higher in laying hens than other treatments. The results of these experiments indicate that starfish would be applied as a feed ingredients if it was properly treated.