• 제목/요약/키워드: standard culture methods

검색결과 239건 처리시간 0.032초

Study on Hygiene Management of Bottled Water through Non-culture-based Total Cell Count (비배양식 총세포수 평가를 통한 병물 위생관리 연구)

  • Se Young Jeong;Min Seo Yang;Eun Su Lee;Sang Yeob Kim;Sung Kyu Maeng
    • Journal of Korean Society on Water Environment
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    • 제40권4호
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    • pp.161-167
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    • 2024
  • The demand for bottled water in South Korea is steadily increasing, but there are challenges regarding water sources and violations of water quality standards. Consumers struggle to identify products that do not meet these standards, highlighting the need for improved water management. This study aims to investigate the use of flow cytometry to identify microbial behavior in bottled water. Twelve different bottled water brands were selected for this study. A novel non-culture-based analysis method called total cell count via flow cytometry was utilized, which is not commonly used to assess drinking water quality. This method was compared to conventional culture-based methods for heterotrophic plate count and E. coli experiments, in order to introduce new indicators for hygiene management. Adenosine triphosphate analysis was also conducted to assess cell activity, and total organic carbon was measured to determine the presence of organic matter. The total cell counts varied among the different bottled water brands. The adenosine triphosphate levels ranged from 37.1ng/L to 221.7ng/L, while the total organic carbon ranged from 0.4 to 0.6 mg/L. Furthermore, E. coli was not detected in any of the bottled waters, and with the exception of two cases, the levels of heterotrophic bacteria did not exceed the drinking water standard of 100 CFU/mL. This study demonstrated a correlation between total cell count and heterotrophic plate count, suggesting that non-culture-based analysis could be valuable in promptly assessing microbial contamination, in contrast to the conventional methods that require approximately 48 hours for incubation.

Shrinkproof Effect and Property of Shrinkproof-Finished Wool Knit

  • Park Myung-Ja;Kwak Soo-Kyoung
    • The International Journal of Costume Culture
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    • 제7권2호
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    • pp.103-111
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    • 2004
  • The shrinkproof-finished wool fibers treated with resin coating and chlorination methods were used to find out an optimal shrinkproof finishing method keeping the quality properties of wool fabric to manufacturers. Shrinkage during repeated washing, electrostatic propensity, thermal resistance and pilling propensity of shrinkproof-finished wool knits, and analysis of finishing methods were measured. Upon the results from the surface examination of shrinkproof-finished wool fibers, the patterns of scale layer and degree of scale removal were subject to change according to the finishing processes. The shrink resistance was significantly enhanced on repeated washing of shrinkproof-finished knits, especially, chlorinated wool. Addition of strong physical force and alkali detergent applied in this washing experiment brought about superior effects with the low shrinkage rate although it was very severe washing conditions for wool fabrics. The results from the washing experiment implies that shrinkproof-finished knitted fabrics can be machine washed at individual households with other ordinary laundry. There was some changes and variation found in thermal resistance, electrostatic propensity, and pilling, however, it seems to be minor within standard limits. Therefore, shrinkproof-finished knitted fabrics did not bring serious changes to other physical properties comparing with original wool, which helps consumers handle wool knitted clothes more conveniently.

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Evaluation of Menu Management and Nutrition Supply in Industrial Foodservice by Food Cost (식품원가 분류에 따른 산업체급식의 식단관리 및 영양공급량평가)

  • Park, Myung-Hee;Choi, Bong-Soon
    • Journal of the Korean Society of Food Culture
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    • 제8권2호
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    • pp.91-101
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    • 1993
  • Unit price of meal provision in industrial foodservice differs with each business. It is believed that menu management work, contents of menu, and nutrition supply performed by dietitian are different with unit price of meal provision. In this sense, purposes of this study are to examine meal management work and contents of menu, by food cost, at industrial foodservice in Taegu, Kyungbuk area and to evaluate variety of menu and nutrition supply. Results of the study are as follows: 1. Average number of side dishes provided at meal, including Kimchi, was 2 at low unit price, 3.0 at middle unit price and 3.1 at high unit price. 2. Possession ratio of account books using for menu management was low. 3. With regard to kind of menu, high unit price was most various and showed more various, compared to low unit price, especially in cooking oil-used cook methods such as broil, pan-fried food, fry, and roast. 4. High unit price was found as the highest in kind of used food and supply volume. 5. Sugar, Mineral, Vitamine, and Protein were satisfied with standard food content, but fat and calcium were not. 6. Supply volume of food was positively correlated to supply volume of nutrient, but kinds of cook methods and food type were not correlated to that of nutrient.

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Safety of Temporary Use of Recycled Autoclaved Femoral Components in Infected Total Knee Arthroplasty: Confirming Sterility Using a Sonication Method

  • Park, Hyung-Jin;Kim, Hee-June;Kim, Shukho;Kim, Seong-Min;Mun, Jong-Uk;Kim, Jungmin;Kyung, Hee-Soo
    • Clinics in Orthopedic Surgery
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    • 제10권4호
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    • pp.427-432
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    • 2018
  • Background: The purpose of this study was to evaluate the usefulness of sonication technique for microbiological diagnosis and the sterility of the recycled autoclaved femoral components from infected total knee arthroplasty (TKA) using a sonication method. Methods: Nineteen femoral implants explanted from patients with infected TKA were sterilized with a standard autoclave method. Standard culture of the fluid before and after sonication of the sterilized implants was performed to detect pathogenic microorganisms. Additional experiments were performed to evaluate the sterility of the recycled implant by inducing artificial biofilm formation. Methicillin-resistant Staphylococcus aureus (MRSA) was inoculated into 10 implants and sterilization in a standard autoclave was performed, and then the fluid was cultured before and after sonication. Results: Two of the 19 sterilized implants were positive for growth of bacteria after sonication, whereas no growth was detected in the cultured fluid from the sterilized implants before sonication. The bacteria were Staphylococcus species in all two cases. In one of 10 implants inoculated with MRSA, the culture was positive for growth of bacteria both before and after sonication. However, Staphylococcus epidermidis was cultured from both occasions and thus this implant was thought to be contaminated. Conclusions: We found sonication for identification of pathogens could be helpful, but this finding should be interpreted carefully because of the possibility of contamination. Sterilization of an infected femoral implant with an autoclave method could be a good method for using the temporary articulating antibiotic spacer in two-stage revision arthroplasty.

A Study on the Service Facilities Plan for APT Community Culture Activation (공동체 문화 활성화를 위한 APT단지 부대복리시설 계획에 관한 연구)

  • Lee, Gwang-Young;Cho, Jin-Man
    • Journal of the Korean housing association
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    • 제19권2호
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    • pp.55-62
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    • 2008
  • This study is researched to provide design guidelines of service facilities and programs that apartment residents want along with the increased quality of life. This study is signifiant because it sought effective methods to activate the com-munity culture through service facilities in apartment complexes and the design standard and planning direction for residents. The study results are as follows. In the result of the correlations analysis and multiple regression analysis for the importancy of service facilities in apartment complexes, the following orders were considered as important factor; man-ager office, open space, resident community facility, elderly people meeting and activity facility, walking paths, resident athletic facility, a children's playground, library, and rest areas in the complex. and result of the correlations analysis and multiple regression analysis is the following: service facilities whole importance (y) = 1.413$+0.262^*{\times}1$ (manager office importance)$+0.271^*{\times}2$ (open space importance) $+0.185^*{\times}3$ (resident community facility importance) $+0.136{\times}4$ (elderly people meeting and activity facility importance)$+0.036^*{\times}5$ (walking paths importance) $+0.031^*{\times}6$ (resident athletic facility importance) + $(-0.042)^*{\times}7$ (a children's playground importance) + $(-0.045)^*{\times}8$ (library importance) + $(-0.0097)^*{\times}9$ (rest areas importance).

Studies on the Distribution of the Mononucleotides in Aspergillus niger during Differentiation (검정곰팡이의 분화에 따르는 모노뉴클레오티드 성분의 분포 변동에 관한 연구)

  • Kim, Boon-Lae;Kim, Jong-Hyup
    • The Korean Journal of Mycology
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    • 제13권2호
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    • pp.65-74
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    • 1985
  • Aspergillus niger IMI 41873 was cultured by the method of synchronous and submerged culture. Its sporulation occurred in the culture. Ribonucleic acids were extracted at each stage of life cycle. These RNAs were digested, separated and determined by P.E.I. cellulose TLC and HPLC methods. The levels of ribonucleic acids in sporulating mycelia were higher than those of conidiophore and phialide forming mycelia. Inosine 5-monophosphate and adenosine 5-monophosphate derivatives were found in HPLC separations. The levels of inosine 5-monophosphate and adenosine 5-monophosphate derivatives per ribonucleic acid were constant through differentiation. After the standard purine necleosides and boiling water extracts from A. bisporus, F. velutipes and L. edodes were added into the culture, their effects on sporulation were examined. Sporulation was greatly enhanced in each adding experiment.

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Comparison of the Standard Culture Method and Real-time PCR for the Detection of Vibrio parahaemolyticus in Seafoods and Vegetables (해산식품과 채소에서 Vibrio parahaemolyticus 검출을 위한 배지배양법과 real-time PCR의 비교검증)

  • Chon, Jung-Whan;Hyeon, Ji-Yeon;Hwang, In-Gyun;Kwak, Hyo-Sun;Han, Jeong-A;Chung, Yun-Hee;Song, Kwang-Young;Seo, Kun-Ho
    • Korean Journal of Food Science and Technology
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    • 제42권3호
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    • pp.355-360
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    • 2010
  • Vibrio parahaemolyticus (V. parahaemolyticus), which is commonly found in raw seafood, causes gastroenteritis in humans. Rapid and effective methods have been developed as culture methods require up to 5-7 days. In this study, real-time PCR was compared with the standard culture method for detecting V. parahaemolyticus in seafood and radish sprout samples. Five hundred grams of the samples were artificially contaminated with V. parahaemolyticus then divided into 20 samples. The samples were incubated in alkaline peptone water and then streaked onto thiosulfate-citrate-bile saltssucrose agar. Biochemical tests for suspicious colonies were performed using the API 20NE strip. In parallel, real-time PCR was performed targeting the toxR gene using the enrichment broth. The real-time PCR was sensitive in discriminating V. parahaemolyticus from other foodborne pathogens. The detection limit of the real-time PCR was $10^3\;CFU/mL$ in phosphate-buffered saline. Although the real-time PCR detected more positive samples (76 out of 180, 42%) than the culture method (66 out of 180, 37%), there was no significant statistical difference (p>0.05) between the two methods. In conclusion, real-time PCR assays could be an alternative to the standard culture method for detecting V. parahaemolyticus in seafood and radish sprouts, which has many advantages in terms of detection time, labor, and sensitivity.

Evaluation of Angle Optimization on Edge Test Device Setting in Modulation Transfer Function (변조전달함수 방법에서 엣지 장치 설정에 대한 각도 최적화 평가)

  • Min, Jung-Whan;Jeong, Hoi-Woun
    • Journal of radiological science and technology
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    • 제43권1호
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    • pp.15-21
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    • 2020
  • This study was purpose to evaluation of Modulation Transfer Function in Measurements by using the International electrotechnical commission standard(IEC 62220-1) which were edge device each angle by using edge method. In this study was Aero(Konica, Japan) image receptor which is a indirect Flat panel detector(FPD) was used. The size of matrix 1994 × 2430 (14"× 17" inch) which performed 12 bit processing and pixel pitch is 175 ㎛. The results of shown as MTF measurements at IEC standard. The amount of data seemed reasonable and at an MTF value of 0.1 the spatial frequencies were 2.56 cycles/mm at an angle of 2.4°. MTF value of 0.5 the spatial frequencies were 1.32 cycles/mm at an angle of 2.4°. This study were to evaluate MTF by setting each angle 2.0°~2.8° degrees the most effective optimal edge angle and to suggest the quantitative methods of measuring by using IEC.

Difference of Microbiology according to Tissue Sampling in Diabetic Ulcers (만성 당뇨발에서 표재조직 및 심부조직 세균배양검사의 비교)

  • Rhee, Sung-Mi;Han, Seung-Kyu;Kim, Woo-Kyung
    • Archives of Plastic Surgery
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    • 제37권1호
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    • pp.1-6
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    • 2010
  • Purpose: Diabetic foot infection is one of the most common and severe complications of diabetes mellitus that delays healing of the wound. Deep tissue biopsy is considered to be the gold standard method for antibiotic selection in treating infected chronic diabetic ulcers. However, swab culture or superficial tissue biopsy is often performed for a microbiologic test since deep tissue biopsy has limitations in application. The purpose of this study is to find out whether microbiologic results of swab culture or superficial tissue biopsy could be used for selection of antibiotics in treating diabetic ulcers. Methods: This study involved 42 patients with diabetic foot ulcers with negative results in bone probing test. Tissue samples for microbiologic tests were collected from all the patients by using superficial cotton swab, superficial tissue, and deep tissue. The microbiologic results of deep tissue biopsy were compared with swab culture and superficial tissue biopsy statistically. Results: Microbiology of the deep tissue showed the same results with those of the swab culture and superficial tissue in 67% and 71%, respectively. Statistical analysis demonstrated that the microbiology of the swab culture and superficial tissue does not coincide with that of the deep tissue. Conclusion: These results suggest that the microbiology of the swab culture and superficial tissue is not concordant with that of the deep tissue in infected chronic diabetic ulcers. To select appropriate antibiotic regimen, the speci specimen for the microbiologic test should be obtained from deep tissue.

A Study on the Origin, Spread, and Universalization of the Name 'Chonggak kimchi': In Connection with the Food Culture Content Point of View ('총각김치' 명칭의 시작과 확산, 그리고 보편화 과정 고찰: 음식문화 콘텐츠 관점을 연계하여)

  • Kim, Hong Ryul
    • Journal of the Korean Society of Food Culture
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    • 제37권5호
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    • pp.418-428
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    • 2022
  • Research on the birth (起源) and names (語源) of foods such as kimchi is important to understand traditional food culture. kimchi, an 'add flavored, fermented, pickled, vegetable food' was initially prepared with the simple purpose of increasing storage capabilities, but later, through a complex process of change, morphological diversification occurred. In addition to the basic name of 'kimchi', each variety has its unique name and history. This study was conducted through qualitative research using various research methods, such as oral records and interviews, as well as investigation of data from literature, including ancient literature, modern cookbooks, newspapers, magazines, papers, and videos. The study sought to investigate the context and the meaning of the name Chonggak kimchi. In addition, it is a compilation of how the name spread through the ages and evolved to its current name. The name Chonggak kimchi did not exist during the Joseon Dynasty and Japanese occupation and first appeared in the records in the late 1950s. Nevertheless, the original name of 'Altarimu kimchi' evolved and finally became a part of the standard Korean language (標準語) in 1988. In the process of the name spreading and becoming popular, the movie "Chonggak kimchi (1964)," starring Shin, S.I., and Eom, A.R. played a significant role. It was also confirmed that this was a meaningful and valuable case of contentization of traditional food culture, regardless of the intention behind the same.